pycom14g03040

Bifunctional purine biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0029451
Physical Location & Seq
Reverse (-)
2356861 .. 2357342
482 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 402 bp
ATGATTTTAAAACCCGTAGCAACGAAGGGGCAGCAATCGCTAGTGTTTACCTGTTTTGTGGCTCTTGCTAAGGAAATCCGAGAGTTTAGAAGCCCGACAGATGGCGAAACCCGGATGTTTTATGAGATTGTGGTTGCACCCAAGTACACAAAGAAATGGCTTGAAATCCTCCGCGGAAAATCAAAGATTCTGAGGATCCTTGAGGCAAGAAACAATGAGAAAGGAAAGCTTTCACTTAGACAGGTTGGTGGTGGGTGGTTAGCTCAGGACTCGGACGATTTGACCCCTCTAGATATACAGTTTAAATTCGCATGGTTGTGCGTCAGGCATGTCAAAAGCAATGCAATTGTAATAACAGAGGTTGTCTTTCTTTGCTTCTCCTTTAAAACCTCGGCTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
AICARFT_IMPCHas MGS

Protein Analysis

134

Amino Acids

15.17

Weight (kDa)

9.54

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AICARFT_IMPCHas PF01808 17 - 120 5.5e-14 AICARFT/IMPCHase bienzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029451)

Species Orthologous Gene IDs
pyrus_communis pycom14g03040
rosa_multiflora Rmu_sc0007023.1_g000006

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 174
AciI CCGC 2 cut(s) 172, 174
AclWI GGATC 2 cut(s) 190, 203
AcsI RAATTY 1 cut(s) 305
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 1 cut(s) 164
AluBI AGCT 2 cut(s) 229, 263
AluI AGCT 2 cut(s) 229, 263
AlwI GGATC 2 cut(s) 190, 203
ApeKI GCWGC 1 cut(s) 31
ApoI RAATTY 1 cut(s) 305
Asp700I GAANNNNTTC 1 cut(s) 229
AsuC2I CCSGG 1 cut(s) 112
BamHI GGATCC 1 cut(s) 195
BbvI GCAGC 1 cut(s) 43
BccI CCATC 1 cut(s) 95
BcnI CCSGG 1 cut(s) 112
BfaI CTAG 2 cut(s) 41, 290
BisI GCNGC 1 cut(s) 32
BlsI GCNGC 1 cut(s) 33
Bme1390I CCNGG 1 cut(s) 112
BmiI GGNNCC 1 cut(s) 197
BmrFI CCNGG 1 cut(s) 112
Bpu10I CCTNAGC 2 cut(s) 69, 264
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 112
BsaJI CCNNGG 2 cut(s) 172, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse3DI GCAATG 1 cut(s) 346
BseDI CCNNGG 2 cut(s) 172, 390
BseGI GGATG 1 cut(s) 120
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 2 cut(s) 182, 278
BseXI GCAGC 1 cut(s) 43
Bsh1236I CGCG 1 cut(s) 174
BsiSI CCGG 1 cut(s) 112
BslI CCNNNNNNNGG 1 cut(s) 101
Bsp143I GATC 1 cut(s) 195
BspACI CCGC 2 cut(s) 172, 174
BspCNI CTCAG 2 cut(s) 183, 277
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 1 cut(s) 197
BspPI GGATC 2 cut(s) 190, 203
BsrDI GCAATG 1 cut(s) 346
BssECI CCNNGG 2 cut(s) 172, 390
BssMI GATC 1 cut(s) 195
Bst4CI ACNGT 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 4 cut(s) 69, 191, 236, 264
BstDSI CCRYGG 1 cut(s) 172
BstF5I GGATG 1 cut(s) 120
BstFNI CGCG 1 cut(s) 174
BstKTI GATC 1 cut(s) 198
BstMBI GATC 1 cut(s) 195
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNSI RCATGY 1 cut(s) 332
BstSCI CCNGG 1 cut(s) 110
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 1 cut(s) 43
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
BtgI CCRYGG 1 cut(s) 172
BtsCI GGATG 1 cut(s) 120
Cac8I GCNNGC 1 cut(s) 397
Cfr42I CCGCGG 1 cut(s) 175
CseI GACGC 1 cut(s) 310
Csp6I GTAC 1 cut(s) 145
CviAII CATG 2 cut(s) 312, 329
CviJI RGCY 6 cut(s) 62, 93, 160, 229, 263, 395
CviKI_1 RGCY 6 cut(s) 62, 93, 160, 229, 263, 395
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 4 cut(s) 69, 191, 236, 264
DpnI GATC 1 cut(s) 197
DpnII GATC 1 cut(s) 195
DraI TTTAAA 3 cut(s) 9, 304, 385
FaeI CATG 2 cut(s) 315, 332
FaiI YATR 4 cut(s) 123, 296, 313, 330
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FatI CATG 2 cut(s) 311, 328
Fnu4HI GCNGC 1 cut(s) 32
FokI GGATG 1 cut(s) 127
Fsp4HI GCNGC 1 cut(s) 32
FspBI CTAG 2 cut(s) 41, 290
GluI GCNGC 1 cut(s) 32
HapII CCGG 1 cut(s) 112
HgaI GACGC 1 cut(s) 310
Hin1II CATG 2 cut(s) 315, 332
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 2 cut(s) 187, 269
HpaII CCGG 1 cut(s) 112
Hpy166II GTNNAC 2 cut(s) 48, 147
Hpy188I TCNGA 3 cut(s) 80, 192, 274
Hpy188III TCNNGA 2 cut(s) 266, 290
Hpy8I GTNNAC 2 cut(s) 48, 147
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 1 cut(s) 300
HpyCH4V TGCA 2 cut(s) 137, 344
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 4 cut(s) 69, 191, 236, 264
Hsp92II CATG 2 cut(s) 315, 332
KspI CCGCGG 1 cut(s) 175
Kzo9I GATC 1 cut(s) 195
LpnPI CCDG 5 cut(s) 64, 125, 227, 251, 310
Lsp1109I GCAGC 1 cut(s) 43
MaeI CTAG 2 cut(s) 41, 290
MalI GATC 1 cut(s) 197
MboI GATC 1 cut(s) 195
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 1 cut(s) 195
MluCI AATT 2 cut(s) 305, 345
MlyI GAGTC 1 cut(s) 263
MnlI CCTC 6 cut(s) 179, 186, 196, 297, 352, 400
MroXI GAANNNNTTC 1 cut(s) 229
MseI TTAA 3 cut(s) 8, 303, 384
MslI CAYNNNNRTG 1 cut(s) 316
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 1 cut(s) 112
MspR9I CCNGG 1 cut(s) 112
MunI CAATTG 1 cut(s) 345
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 37
NciI CCSGG 1 cut(s) 112
NdeII GATC 1 cut(s) 195
NlaIII CATG 2 cut(s) 315, 332
NlaIV GGNNCC 1 cut(s) 197
NmeAIII GCCGAG 1 cut(s) 371
NspI RCATGY 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 229
PfeI GAWTC 1 cut(s) 187
PkrI GCNGC 1 cut(s) 33
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
PspN4I GGNNCC 1 cut(s) 197
PsuI RGATCY 1 cut(s) 195
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 316
SacII CCGCGG 1 cut(s) 175
SaqAI TTAA 3 cut(s) 8, 303, 384
SatI GCNGC 1 cut(s) 32
Sau3AI GATC 1 cut(s) 195
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 1 cut(s) 112
SetI ASST 6 cut(s) 53, 231, 246, 265, 363, 392
Sfr303I CCGCGG 1 cut(s) 175
SgrBI CCGCGG 1 cut(s) 175
SmiMI CAYNNNNRTG 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 2 cut(s) 305, 345
SsiI CCGC 2 cut(s) 172, 174
SspMI CTAG 2 cut(s) 41, 290
StyD4I CCNGG 1 cut(s) 110
TaaI ACNGT 1 cut(s) 300
TasI AATT 2 cut(s) 305, 345
TatI WGTACW 1 cut(s) 144
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 3 cut(s) 8, 303, 384
Tru9I TTAA 3 cut(s) 8, 303, 384
TseI GCWGC 1 cut(s) 31
XapI RAATTY 1 cut(s) 305
XbaI TCTAGA 1 cut(s) 289
XceI RCATGY 1 cut(s) 332
XmnI GAANNNNTTC 1 cut(s) 229
XspI CTAG 2 cut(s) 41, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.