pycom14g04400

Colon cancer-associated protein Mic1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012938
Physical Location & Seq
Reverse (-)
3906649 .. 3911313
4665 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1749 bp
ATGGTTCTTCTTGCTTCAGGAATGCAGTGTAAAACCTTCAATGGTTTTCAGCTCTCGTCTGCAGGCATTATTCGTCTGCCAAAGTTTGAGATGGCAATGGCTAAATCCGAAGCTAACAATAAACCTGTCCTTGCTGCTGAAGATGTTTTTATAGTCACTGTCTATGGCAGAATATACTGCCTGCAAGTTGATAGGATTGCAATGCTCCTCCATTCTTACAGGTTTTACCGTGATGTGGTTGTACAACAGGGTTCTTTGCCAATTTATTCAAGCAAGGTTGCTGTGAGTGTGGTTGATAATGTACTGCTTGTGCATCAAGTTGATGCGAAGGTTGTTATACTGTATGACATATTTGCAGATTCCCAAGCACCCATATCCGCCCCACTTCCCCTATTATTCAGGGGTTTCCCCAGGTCTAATTCTTCTTCCCAGAGATCCAATAGGGACGATAACGAAAGTTCAGAAGTTAATGTTTTAAGTGATCATGAAGCAATTGTCTATGGAGATAATTGGACTTTTCTTGTTCCTGACCTCATCTGTGATGTTGCTAATCAACTTTTGTGGAAGATTCATTTAGATTTGGAGGCAATTTCTGCCAGTAGCTCAGAAGTACCATCAGTACTTGAATTCTTGCAACGGCGGAAGTTAGAAGCGAATAAGGCTAAACAACTGTGCTTATCAATAGCACGCACTGTTATTCTAGAACGGAGACCTGTGTCTACAGTTTCCTGGGCATTAGATGTTTTAGTTTCCTCGTACTCCCACTCTGTTAAGACAGGCACTTATCTTAAGGGAATAAATTCTGCAAAAACTTCTGGTGAGCCACAGACGAGTGTCCCTGGATCTAGTGCTGATGTATCTTCTAGAAGAGTAGACACAGTTGGGAAATCCGTTAAATATGAATCTGCTGCTGGATTGGACAGTGAATCGCCTAATAGATTTTTAACCTATTCAAATTCCGACTCTGAAGATAATGCAAGCTTTGAAGCACCAAATGCCACTTCAAATAACTCTCAGTTGTTCGATGGTAAAGTGGACATGGGGAAGTTAACCGGTGCTGAAACTTCTAGCGGTGAGGTTCGGTCTTCATCGTTGCAATATCAGGTTCTTAGATCGGGCAACAGCTCATTGGATGCTAATGGTTCAGAGCAGCAAGAGTCCCAACCGACCTCTCCAGCAATTTCACCTGATGAAATGTACAGTTTTGTGTTTGCACCTGTTGAAGAAGAGATTATTGGAGAACCTTCGTACTTGGTTGCCATCATTGTTGAGTTTCTTCGCAGTGCTAATCTGGAAAAGGTTGAAGTCCATCCTAATCTCTATGTCCTGACCGTACAATTGCTGTCACACAGTGAGCGATATGCAGAACTTGGGCAGTTTGTCATAAACAAGATTCTTGAACCTTCTAAAGAAGTAGCAATGCAACTCCTAGAGTCTGGCCGTCAGAACTCTCGAACAAGGAAGTTGGGTTTAGATATGCTGAGGCAGCTTTCTTTACATCATGACTATGTACTGTTACTAGTGCAAGATGGATATTACCTTGAAGCATTACGCTATGCACGGAAGTACAAGGTCAGTACCATCCGACCTTCGCTGTTTCTTGAGTCAGCATTTACCTACAACGATTCGCAACATTTAGCTGCAGTCCTCAGATTCTTTTCGGATTTCATTCCTGGATTCAGAGACACATCAGATCACGACACATATTTCCGCATTCTCAACGAGAGGAACTCATCCATTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mic1

Protein Analysis

583

Amino Acids

64.62

Weight (kDa)

5.45

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RMC1_C PF07035 374 - 555 2.6e-46 Regulator of MON1-CCZ1 complex, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012938)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12010
fragaria_vesca FvH4_6g21770
malus_domestica MD12G1054100.v1.1 MD14G1053000.v1.1
prunus_persica Prupe.7G082700_v2.0.a1
pyrus_communis pycom12g04720 pycom14g04400
rosa_chinensis RchiOBHm_Chr3g0479121
rosa_laevigata RLG00000023605
rosa_multiflora Rmu_sc0000038.1_g000003
rosa_roxburghii Rroxscaffold_6G00402710
rosa_rugosa Rorug03G0171200 Rorug03G0171300
rosa_samantha Rh3AG222300 Rh3BG254800 Rh3CG250500 Rh3DG246800
rosa_wichuraiana Rw3G019930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 719, 873
AciI CCGC 4 cut(s) 378, 640, 1071, 1711
AclWI GGATC 2 cut(s) 429, 850
AcoI YGGCCR 1 cut(s) 1438
AcsI RAATTY 3 cut(s) 626, 799, 955
AcuI CTGAAG 2 cut(s) 159, 987
AdeI CACNNNGTG 1 cut(s) 1352
AfiI CCNNNNNNNGG 1 cut(s) 235
AflII CTTAAG 1 cut(s) 788
AgeI ACCGGT 1 cut(s) 1052
AhlI ACTAGT 1 cut(s) 1519
AjnI CCWGG 4 cut(s) 410, 728, 838, 1672
AjuI GAANNNNNNNTTGG 4 cut(s) 985, 1017, 1218, 1250
AluBI AGCT 7 cut(s) 52, 113, 603, 981, 1125, 1489, 1640
AluI AGCT 7 cut(s) 52, 113, 603, 981, 1125, 1489, 1640
Alw26I GTCTC 2 cut(s) 703, 1677
AlwI GGATC 2 cut(s) 429, 850
AoxI GGCC 1 cut(s) 1438
ApeKI GCWGC 6 cut(s) 134, 908, 1150, 1486, 1640, 1742
ApoI RAATTY 3 cut(s) 626, 799, 955
AsiGI ACCGGT 1 cut(s) 1052
Asp700I GAANNNNTTC 1 cut(s) 799
AsuHPI GGTGA 3 cut(s) 830, 1085, 1176
BbsI GAAGAC 1 cut(s) 1077
BbvCI CCTCAGC 1 cut(s) 1481
BbvI GCAGC 6 cut(s) 121, 895, 1162, 1498, 1627, 1729
BccI CCATC 7 cut(s) 85, 622, 1019, 1268, 1317, 1523, 1589
BceAI ACGGC 2 cut(s) 653, 1425
BciT130I CCWGG 4 cut(s) 412, 730, 840, 1674
BclI TGATCA 1 cut(s) 481
BcoDI GTCTC 2 cut(s) 703, 1677
BcuI ACTAGT 1 cut(s) 1519
BfaI CTAG 6 cut(s) 701, 846, 864, 1068, 1430, 1520
BfmI CTRYAG 3 cut(s) 60, 720, 1641
BfrI CTTAAG 1 cut(s) 788
BisI GCNGC 6 cut(s) 135, 909, 1151, 1487, 1641, 1743
BlsI GCNGC 6 cut(s) 136, 910, 1152, 1488, 1642, 1744
BmcAI AGTACT 1 cut(s) 621
Bme1390I CCNGG 4 cut(s) 412, 730, 840, 1674
BmrFI CCNGG 4 cut(s) 412, 730, 840, 1674
BmsI GCATC 3 cut(s) 313, 322, 1123
BoxI GACNNNNGTC 2 cut(s) 715, 833
BpiI GAAGAC 1 cut(s) 1077
BplI GAGNNNNNCTC 2 cut(s) 1715, 1747
BpmI CTGGAG 1 cut(s) 1158
Bpu10I CCTNAGC 1 cut(s) 1481
BpuEI CTTGAG 1 cut(s) 1622
BsaI GGTCTC 1 cut(s) 703
BsaJI CCNNGG 3 cut(s) 410, 729, 838
BsaWI WCCGGW 1 cut(s) 1052
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse118I RCCGGY 1 cut(s) 1052
Bse1I ACTGG 1 cut(s) 597
Bse3DI GCAATG 4 cut(s) 102, 207, 1425, 1737
BseBI CCWGG 4 cut(s) 412, 730, 840, 1674
BseDI CCNNGG 3 cut(s) 410, 729, 838
BseGI GGATG 4 cut(s) 1138, 1309, 1581, 1733
BseLI CCNNNNNNNGG 1 cut(s) 235
BseMI GCAATG 4 cut(s) 102, 207, 1425, 1737
BseMII CTCAG 4 cut(s) 618, 1028, 1472, 1663
BseNI ACTGG 1 cut(s) 597
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 6 cut(s) 121, 895, 1162, 1498, 1627, 1729
BshFI GGCC 1 cut(s) 1440
BshTI ACCGGT 1 cut(s) 1052
BsiSI CCGG 1 cut(s) 1053
BslFI GGGAC 3 cut(s) 458, 821, 1144
BslI CCNNNNNNNGG 1 cut(s) 235
BsmAI GTCTC 2 cut(s) 703, 1677
BsmFI GGGAC 3 cut(s) 458, 821, 1144
BsmI GAATGC 2 cut(s) 27, 1713
BsnI GGCC 1 cut(s) 1440
Bso31I GGTCTC 1 cut(s) 703
Bsp1407I TGTACA 2 cut(s) 241, 1197
Bsp143I GATC 5 cut(s) 434, 481, 842, 1112, 1693
BspACI CCGC 4 cut(s) 378, 640, 1071, 1711
BspANI GGCC 1 cut(s) 1440
BspCNI CTCAG 4 cut(s) 617, 1027, 1473, 1662
BspHI TCATGA 2 cut(s) 484, 1501
BspMAI CTGCAG 2 cut(s) 64, 1645
BspPI GGATC 2 cut(s) 429, 850
BspTI CTTAAG 1 cut(s) 788
BspTNI GGTCTC 1 cut(s) 703
BsrDI GCAATG 4 cut(s) 102, 207, 1425, 1737
BsrFI RCCGGY 1 cut(s) 1052
BsrGI TGTACA 2 cut(s) 241, 1197
BsrI ACTGG 1 cut(s) 597
BssAI RCCGGY 1 cut(s) 1052
BssECI CCNNGG 3 cut(s) 410, 729, 838
BssMI GATC 5 cut(s) 434, 481, 842, 1112, 1693
Bst2UI CCWGG 4 cut(s) 412, 730, 840, 1674
Bst6I CTCTTC 2 cut(s) 862, 1221
BstAFI CTTAAG 1 cut(s) 788
BstAPI GCANNNNNTGC 2 cut(s) 593, 995
BstAUI TGTACA 2 cut(s) 241, 1197
BstC8I GCNNGC 4 cut(s) 64, 182, 688, 979
BstDEI CTNAG 5 cut(s) 604, 1014, 1109, 1481, 1649
BstF5I GGATG 4 cut(s) 1138, 1309, 1581, 1733
BstKTI GATC 5 cut(s) 437, 484, 845, 1115, 1696
BstMAI GTCTC 2 cut(s) 703, 1677
BstMBI GATC 5 cut(s) 434, 481, 842, 1112, 1693
BstMWI GCNNNNNNNGC 4 cut(s) 593, 659, 995, 1486
BstNI CCWGG 4 cut(s) 412, 730, 840, 1674
BstPAI GACNNNNGTC 2 cut(s) 715, 833
BstSCI CCNGG 4 cut(s) 410, 728, 838, 1672
BstSFI CTRYAG 3 cut(s) 60, 720, 1641
BstV1I GCAGC 6 cut(s) 121, 895, 1162, 1498, 1627, 1729
BstV2I GAAGAC 1 cut(s) 1077
BstX2I RGATCY 2 cut(s) 434, 842
BstYI RGATCY 2 cut(s) 434, 842
BsuRI GGCC 1 cut(s) 1440
BtsCI GGATG 4 cut(s) 1138, 1309, 1581, 1733
BtsI GCAGTG 2 cut(s) 32, 1288
BtsIMutI CAGTG 6 cut(s) 32, 156, 690, 928, 1288, 1357
Cac8I GCNNGC 4 cut(s) 64, 182, 688, 979
CciI TCATGA 2 cut(s) 484, 1501
Cfr10I RCCGGY 1 cut(s) 1052
CspAI ACCGGT 1 cut(s) 1052
CspCI CAANNNNNGTGG 2 cut(s) 542, 577
CviAII CATG 3 cut(s) 485, 1039, 1502
DdeI CTNAG 5 cut(s) 604, 1014, 1109, 1481, 1649
DpnI GATC 5 cut(s) 436, 483, 844, 1114, 1695
DpnII GATC 5 cut(s) 434, 481, 842, 1112, 1693
DraIII CACNNNGTG 1 cut(s) 1352
EaeI YGGCCR 1 cut(s) 1438
Eam1104I CTCTTC 2 cut(s) 862, 1221
EarI CTCTTC 2 cut(s) 862, 1221
EciI GGCGGA 2 cut(s) 367, 655
Eco31I GGTCTC 1 cut(s) 703
Eco57I CTGAAG 2 cut(s) 159, 987
EcoRI GAATTC 1 cut(s) 626
EcoRII CCWGG 4 cut(s) 410, 728, 838, 1672
FaeI CATG 3 cut(s) 488, 1042, 1505
FaqI GGGAC 3 cut(s) 458, 821, 1144
FatI CATG 3 cut(s) 484, 1038, 1501
FbaI TGATCA 1 cut(s) 481
FblI GTMKAC 2 cut(s) 719, 873
Fnu4HI GCNGC 6 cut(s) 135, 909, 1151, 1487, 1641, 1743
FokI GGATG 4 cut(s) 1145, 1296, 1568, 1720
Fsp4HI GCNGC 6 cut(s) 135, 909, 1151, 1487, 1641, 1743
FspBI CTAG 6 cut(s) 701, 846, 864, 1068, 1430, 1520
GluI GCNGC 6 cut(s) 135, 909, 1151, 1487, 1641, 1743
GsuI CTGGAG 1 cut(s) 1158
HaeIII GGCC 1 cut(s) 1440
HapII CCGG 1 cut(s) 1053
Hin1II CATG 3 cut(s) 488, 1042, 1505
HincII GTYRAC 1 cut(s) 1050
HindII GTYRAC 1 cut(s) 1050
HindIII AAGCTT 1 cut(s) 979
HpaI GTTAAC 1 cut(s) 1050
HpaII CCGG 1 cut(s) 1053
HphI GGTGA 3 cut(s) 830, 1085, 1176
Hpy166II GTNNAC 4 cut(s) 720, 874, 1036, 1050
Hpy8I GTNNAC 4 cut(s) 720, 874, 1036, 1050
HpyAV CCTTC 5 cut(s) 46, 322, 1254, 1413, 1599
HpyF10VI GCNNNNNNNGC 4 cut(s) 593, 659, 995, 1486
HpyF3I CTNAG 5 cut(s) 604, 1014, 1109, 1481, 1649
Hsp92II CATG 3 cut(s) 488, 1042, 1505
Ksp22I TGATCA 1 cut(s) 481
KspAI GTTAAC 1 cut(s) 1050
Kzo9I GATC 5 cut(s) 434, 481, 842, 1112, 1693
LmnI GCTCC 1 cut(s) 210
Lsp1109I GCAGC 6 cut(s) 121, 895, 1162, 1498, 1627, 1729
LweI GCATC 3 cut(s) 313, 322, 1123
MaeI CTAG 6 cut(s) 701, 846, 864, 1068, 1430, 1520
MaeIII GTNAC 3 cut(s) 154, 1344, 1515
MalI GATC 5 cut(s) 436, 483, 844, 1114, 1695
MboI GATC 5 cut(s) 434, 481, 842, 1112, 1693
MfeI CAATTG 2 cut(s) 492, 1337
MflI RGATCY 2 cut(s) 434, 842
MlyI GAGTC 4 cut(s) 956, 1166, 1442, 1613
MmeI TCCRAC 2 cut(s) 984, 1609
MnlI CCTC 9 cut(s) 218, 542, 577, 763, 1069, 1180, 1476, 1658, 1719
MroXI GAANNNNTTC 1 cut(s) 799
MseI TTAA 7 cut(s) 468, 476, 771, 789, 894, 944, 1049
MslI CAYNNNNRTG 1 cut(s) 1506
MspCI CTTAAG 1 cut(s) 788
MspI CCGG 1 cut(s) 1053
MspR9I CCNGG 4 cut(s) 412, 730, 840, 1674
MunI CAATTG 2 cut(s) 492, 1337
Mva1269I GAATGC 2 cut(s) 27, 1713
MvaI CCWGG 4 cut(s) 412, 730, 840, 1674
MwoI GCNNNNNNNGC 4 cut(s) 593, 659, 995, 1486
NdeII GATC 5 cut(s) 434, 481, 842, 1112, 1693
NlaIII CATG 3 cut(s) 488, 1042, 1505
NmuCI GTSAC 2 cut(s) 154, 1344
PagI TCATGA 2 cut(s) 484, 1501
PctI GAATGC 2 cut(s) 27, 1713
PdmI GAANNNNTTC 1 cut(s) 799
PfeI GAWTC 8 cut(s) 359, 568, 902, 926, 1393, 1625, 1653, 1677
PfoI TCCNGGA 1 cut(s) 1672
PinAI ACCGGT 1 cut(s) 1052
PkrI GCNGC 6 cut(s) 136, 910, 1152, 1488, 1642, 1744
PleI GAGTC 4 cut(s) 956, 1165, 1441, 1612
PpsI GAGTC 4 cut(s) 956, 1165, 1441, 1612
PshAI GACNNNNGTC 2 cut(s) 715, 833
Psp6I CCWGG 4 cut(s) 410, 728, 838, 1672
PspGI CCWGG 4 cut(s) 410, 728, 838, 1672
PstI CTGCAG 2 cut(s) 64, 1645
PsuI RGATCY 2 cut(s) 434, 842
RseI CAYNNNNRTG 1 cut(s) 1506
SaqAI TTAA 7 cut(s) 468, 476, 771, 789, 894, 944, 1049
SatI GCNGC 6 cut(s) 135, 909, 1151, 1487, 1641, 1743
Sau3AI GATC 5 cut(s) 434, 481, 842, 1112, 1693
ScaI AGTACT 1 cut(s) 621
SchI GAGTC 4 cut(s) 956, 1166, 1442, 1613
ScrFI CCNGG 4 cut(s) 412, 730, 840, 1674
SfaNI GCATC 3 cut(s) 313, 322, 1123
SfcI CTRYAG 3 cut(s) 60, 720, 1641
SmiMI CAYNNNNRTG 1 cut(s) 1506
SmlI CTYRAG 2 cut(s) 788, 1601
SmoI CTYRAG 2 cut(s) 788, 1601
SpeI ACTAGT 1 cut(s) 1519
SsiI CCGC 4 cut(s) 378, 640, 1071, 1711
SspMI CTAG 6 cut(s) 701, 846, 864, 1068, 1430, 1520
StyD4I CCNGG 4 cut(s) 410, 728, 838, 1672
TaqI TCGA 2 cut(s) 1023, 1453
TaqII GACCGA 1 cut(s) 1071
TatI WGTACW 6 cut(s) 241, 301, 619, 1197, 1510, 1566
TfiI GAWTC 8 cut(s) 359, 568, 902, 926, 1393, 1625, 1653, 1677
Tru1I TTAA 7 cut(s) 468, 476, 771, 789, 894, 944, 1049
Tru9I TTAA 7 cut(s) 468, 476, 771, 789, 894, 944, 1049
TscAI CASTG 6 cut(s) 32, 163, 697, 928, 1288, 1357
TseFI GTSAC 2 cut(s) 154, 1344
TseI GCWGC 6 cut(s) 134, 908, 1150, 1486, 1640, 1742
Tsp45I GTSAC 2 cut(s) 154, 1344
TspDTI ATGAA 6 cut(s) 501, 560, 915, 1077, 1206, 1657
TspGWI ACGGA 3 cut(s) 721, 880, 1576
TspRI CASTG 6 cut(s) 32, 163, 697, 928, 1288, 1357
Vha464I CTTAAG 1 cut(s) 788
XapI RAATTY 3 cut(s) 626, 799, 955
XbaI TCTAGA 2 cut(s) 700, 863
XmiI GTMKAC 2 cut(s) 719, 873
XmnI GAANNNNTTC 1 cut(s) 799
XspI CTAG 6 cut(s) 701, 846, 864, 1068, 1430, 1520
ZrmI AGTACT 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.