Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0028324

Physical Location & Seq

Genomic Coordinates Reverse (-)

5264371 .. 5267028

Gene Structure 2658 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 249 bp

ATGACACTGAATACTTTCCATCTTGCCGGAAGAGGTGAAATGAATGTGACCCTTGGAATCCCTCATCTTCAAGAGATTTTGATGACTGCTGCAAATGAGATTAAGACTCCGGTTATGACATGTCCATTGCAAAAAGGGAGATCAAAGGAGGAAGCTCAGCATCTAGCTGATAAGCTGAAGAAGATCACTGTGGCAGACTTAATGGAGTATGGAAGTTACTTTGTTGCCATATGTCCTTCAGGGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005911 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

83

Amino Acids

8.99

Weight (kDa)

6.81

Isoelectric Point (pI)

31.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_5	PF04998	1 - 57	1e-10	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0028324)

Species	Orthologous Gene IDs
malus_domestica	MD14G1066800.v1.1
pyrus_communis	pycom14g05420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AcuI	CTGAAG	2 cut(s)	197, 222
AflIII	ACRYGT	1 cut(s)	119
AgsI	TTSAA	1 cut(s)	71
AluBI	AGCT	3 cut(s)	155, 167, 175
AluI	AGCT	3 cut(s)	155, 167, 175
ApeKI	GCWGC	1 cut(s)	89
Asp700I	GAANNNNTTC	1 cut(s)	14
AsuHPI	GGTGA	1 cut(s)	47
BbvI	GCAGC	1 cut(s)	76
BccI	CCATC	1 cut(s)	27
BfaI	CTAG	1 cut(s)	164
BisI	GCNGC	1 cut(s)	90
BlpI	GCTNAGC	1 cut(s)	156
BlsI	GCNGC	1 cut(s)	91
BmsI	GCATC	1 cut(s)	169
Bpu1102I	GCTNAGC	1 cut(s)	156
BsaJI	CCNNGG	1 cut(s)	52
BsaWI	WCCGGW	1 cut(s)	109
Bse3DI	GCAATG	1 cut(s)	125
BseDI	CCNNGG	1 cut(s)	52
BseMI	GCAATG	1 cut(s)	125
BseMII	CTCAG	1 cut(s)	170
BseXI	GCAGC	1 cut(s)	76
BsiSI	CCGG	2 cut(s)	27, 110
Bsp143I	GATC	2 cut(s)	140, 183
Bsp1720I	GCTNAGC	1 cut(s)	156
BspCNI	CTCAG	1 cut(s)	169
BsrDI	GCAATG	1 cut(s)	125
BssECI	CCNNGG	1 cut(s)	52
BssMI	GATC	2 cut(s)	140, 183
BssT1I	CCWWGG	1 cut(s)	52
Bst4CI	ACNGT	1 cut(s)	190
Bst6I	CTCTTC	1 cut(s)	25
BstDEI	CTNAG	1 cut(s)	156
BstKTI	GATC	2 cut(s)	143, 186
BstMBI	GATC	2 cut(s)	140, 183
BstNSI	RCATGY	1 cut(s)	123
BstV1I	GCAGC	1 cut(s)	76
BtsIMutI	CAGTG	2 cut(s)	5, 186
CviAII	CATG	1 cut(s)	120
CviJI	RGCY	3 cut(s)	155, 167, 175
CviKI_1	RGCY	3 cut(s)	155, 167, 175
DdeI	CTNAG	1 cut(s)	156
DpnI	GATC	2 cut(s)	142, 185
DpnII	GATC	2 cut(s)	140, 183
Eam1104I	CTCTTC	1 cut(s)	25
EarI	CTCTTC	1 cut(s)	25
Eco130I	CCWWGG	1 cut(s)	52
Eco57I	CTGAAG	2 cut(s)	197, 222
EcoT14I	CCWWGG	1 cut(s)	52
ErhI	CCWWGG	1 cut(s)	52
FaeI	CATG	1 cut(s)	123
FaiI	YATR	5 cut(s)	116, 121, 210, 230, 232
FatI	CATG	1 cut(s)	119
FauNDI	CATATG	1 cut(s)	230
Fnu4HI	GCNGC	1 cut(s)	90
Fsp4HI	GCNGC	1 cut(s)	90
FspBI	CTAG	1 cut(s)	164
GluI	GCNGC	1 cut(s)	90
HapII	CCGG	2 cut(s)	27, 110
Hin1II	CATG	1 cut(s)	123
HinfI	GANTC	2 cut(s)	57, 106
HpaII	CCGG	2 cut(s)	27, 110
HphI	GGTGA	1 cut(s)	47
Hpy188III	TCNNGA	2 cut(s)	71, 246
HpyAV	CCTTC	1 cut(s)	246
HpyCH4III	ACNGT	1 cut(s)	190
HpyCH4V	TGCA	2 cut(s)	92, 130
HpyF3I	CTNAG	1 cut(s)	156
Hsp92II	CATG	1 cut(s)	123
Kzo9I	GATC	2 cut(s)	140, 183
LpnPI	CCDG	3 cut(s)	40, 123, 225
Lsp1109I	GCAGC	1 cut(s)	76
LweI	GCATC	1 cut(s)	169
MaeI	CTAG	1 cut(s)	164
MaeIII	GTNAC	2 cut(s)	46, 215
MalI	GATC	2 cut(s)	142, 185
MboI	GATC	2 cut(s)	140, 183
MboII	GAAGA	4 cut(s)	42, 59, 190, 193
MlyI	GAGTC	1 cut(s)	100
MnlI	CCTC	3 cut(s)	26, 72, 142
MroXI	GAANNNNTTC	1 cut(s)	14
MseI	TTAA	2 cut(s)	102, 200
MspI	CCGG	2 cut(s)	27, 110
NdeI	CATATG	1 cut(s)	230
NdeII	GATC	2 cut(s)	140, 183
NlaIII	CATG	1 cut(s)	123
NmuCI	GTSAC	1 cut(s)	46
NspI	RCATGY	1 cut(s)	123
PciI	ACATGT	1 cut(s)	119
PdmI	GAANNNNTTC	1 cut(s)	14
PfeI	GAWTC	1 cut(s)	57
PkrI	GCNGC	1 cut(s)	91
PleI	GAGTC	1 cut(s)	100
PpsI	GAGTC	1 cut(s)	100
PscI	ACATGT	1 cut(s)	119
SaqAI	TTAA	2 cut(s)	102, 200
SatI	GCNGC	1 cut(s)	90
Sau3AI	GATC	2 cut(s)	140, 183
SchI	GAGTC	1 cut(s)	100
SetI	ASST	4 cut(s)	37, 157, 169, 177
SfaNI	GCATC	1 cut(s)	169
SgeI	CNNG	7 cut(s)	35, 39, 65, 83, 122, 132, 176
SspMI	CTAG	1 cut(s)	164
StyI	CCWWGG	1 cut(s)	52
TaaI	ACNGT	1 cut(s)	190
TfiI	GAWTC	1 cut(s)	57
Tru1I	TTAA	2 cut(s)	102, 200
Tru9I	TTAA	2 cut(s)	102, 200
TscAI	CASTG	2 cut(s)	12, 193
TseFI	GTSAC	1 cut(s)	46
TseI	GCWGC	1 cut(s)	89
Tsp45I	GTSAC	1 cut(s)	46
TspDTI	ATGAA	1 cut(s)	56
TspRI	CASTG	2 cut(s)	12, 193
XceI	RCATGY	1 cut(s)	123
XmnI	GAANNNNTTC	1 cut(s)	14
XspI	CTAG	1 cut(s)	164

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.