pycom14g08770

histone-lysine N-methyltransferase, H3 lysine-9 specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0024693
Physical Location & Seq
Forward (+)
10021157 .. 10022266
1110 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1065 bp
ATGGAACAAAGTTTAGGTCAAGAGTCTGTTCCTGGTTTTGGGTCACTTGACAAGTCTAAGGTTTTAGATGTAAGGCCTTTGAGACGTCTTGTTCCGTTGTTCCCGTCAGCTTCTAATAATACTTCATTTTCAACTCCACAAGGTGCTGCTCCTTTCGTTTGTGTTTCTCCTGCTGGCCCTTTCCCACCTGGGGTTGCCCCATTTTTTCCGTTCTTCATTTCACCGGAGTCGCAAAGGCCACTTGAACAAAATCTGCAAACACCAATCCCAATTAATTCATTTAGGACGCCTCCATCCGCAGACGCTAATGGAGATACGAGGACAGCAAGAAGGCTTGCTCGAAGTCGTGGTCAGCCACAATCCCAAAGTGGGAATGCAGAGGAAGATGGTTTTGATGGAGAAAATACAAGGAAGGTAGGGAGCTCAAGGAGTAAGTTCCGGTCCCAAAAGAGGACAAGGGGTGGCCAGGACATTAATGTTGCTTTGCCTGATGTTGACATTGATGCAATAATTGATAATGTTCTTACAACATATACCAATACATTCCAACAAACTGATAGTGACAGGGAATCGGTTGTATATGCACTCACGTCCTATGACTTGGTGCGAAGAAGGATTACACAAATTGAAGAGATGAAGGAAAGAATACCAGGAGCCCCAAGAACAGGACGCCCTGATCTAAGGACAGGAACACTCTTCATGAATAAGGGAATTAGGACAAATGCCAAGAAGAGGACTGGTGCAGTGCCTGGTGTTGAAGTCGGGGATATTTTCTTTTTCCGAATGGAACTGTGCTTGGTTGGATTACATGCTCCAACTATGGGTGGAATTGATTACATGGGAGTCAAGAACAGTACAGAGGAAGAGCCTTTAGCATTGAGCATTGTTTCTTCTGGAGGATATGAGGATTCTGTGGAGGATTCGAATGTGTTGATATACAGTGGCCAAGGTGGTAATGCTAGCAATGATTTCAGGAGAGATAAGGAAATGATGATGATCAGAAGCTTGAAAGGGGTAATCTTGCTTTGGAGAAAAGTTTGCATCGGGGTAATGATGTCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00310 ko01100 ko04146 ko04211 map00260 map00310 map01100 map04146 map04211
Pfam Domains
Protein Families
AT_hook Pre-SET SAD_SRA SET

Protein Analysis

355

Amino Acids

38.85

Weight (kDa)

8.39

Isoelectric Point (pI)

61.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 246 - 323 6.5e-24 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024693)

Species Orthologous Gene IDs
pyrus_communis pycom14g08770
rosa_chinensis RchiOBHm_Chr3g0494331
rosa_samantha Rh3AG314900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 88
AciI CCGC 1 cut(s) 297
AcoI YGGCCR 2 cut(s) 463, 943
AcyI GRCGYC 3 cut(s) 85, 287, 670
AdeI CACNNNGTG 1 cut(s) 143
AfaI GTAC 1 cut(s) 856
AfiI CCNNNNNNNGG 7 cut(s) 38, 190, 369, 450, 665, 732, 821
AgsI TTSAA 5 cut(s) 132, 245, 629, 758, 1009
AjiI CACGTC 1 cut(s) 591
AjnI CCWGG 5 cut(s) 31, 187, 465, 649, 748
AluBI AGCT 3 cut(s) 110, 423, 1005
AluI AGCT 3 cut(s) 110, 423, 1005
Alw21I GWGCWC 1 cut(s) 425
Alw26I GTCTC 1 cut(s) 76
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 5 cut(s) 74, 175, 236, 463, 943
ApeKI GCWGC 1 cut(s) 146
ArsI GACNNNNNNTTYG 3 cut(s) 33, 334, 366
AseI ATTAAT 2 cut(s) 273, 474
AspS9I GGNCC 2 cut(s) 176, 441
AsuHPI GGTGA 1 cut(s) 213
AsuII TTCGAA 1 cut(s) 923
AsuNHI GCTAGC 1 cut(s) 959
AvaII GGWCC 1 cut(s) 441
BalI TGGCCA 2 cut(s) 465, 945
BanII GRGCYC 2 cut(s) 425, 658
BarI GAAGNNNNNNTAC 2 cut(s) 601, 633
Bbv12I GWGCWC 1 cut(s) 425
BbvI GCAGC 1 cut(s) 133
BccI CCATC 3 cut(s) 301, 380, 389
BciT130I CCWGG 5 cut(s) 33, 189, 467, 651, 750
BclI TGATCA 1 cut(s) 996
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 1 cut(s) 960
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 5 cut(s) 33, 189, 467, 651, 750
Bme18I GGWCC 1 cut(s) 441
BmgBI CACGTC 1 cut(s) 591
BmgT120I GGNCC 2 cut(s) 176, 441
BmiI GGNNCC 2 cut(s) 443, 655
BmrFI CCNGG 5 cut(s) 33, 189, 467, 651, 750
BmsI GCATC 2 cut(s) 493, 1050
BmtI GCTAGC 1 cut(s) 963
BpmI CTGGAG 1 cut(s) 915
Bpu14I TTCGAA 1 cut(s) 923
BpuEI CTTGAG 1 cut(s) 409
BsaBI GATNNNNATC 1 cut(s) 995
BsaHI GRCGYC 3 cut(s) 85, 287, 670
BsaJI CCNNGG 2 cut(s) 188, 946
BsaWI WCCGGW 2 cut(s) 223, 438
Bsc4I CCNNNNNNNGG 7 cut(s) 38, 190, 369, 450, 665, 732, 821
Bse1I ACTGG 1 cut(s) 742
Bse3DI GCAATG 1 cut(s) 970
Bse8I GATNNNNATC 1 cut(s) 995
BseBI CCWGG 5 cut(s) 33, 189, 467, 651, 750
BseDI CCNNGG 2 cut(s) 188, 946
BseGI GGATG 1 cut(s) 293
BseJI GATNNNNATC 1 cut(s) 995
BseLI CCNNNNNNNGG 7 cut(s) 38, 190, 369, 450, 665, 732, 821
BseMI GCAATG 1 cut(s) 970
BseNI ACTGG 1 cut(s) 742
BseXI GCAGC 1 cut(s) 133
BsgI GTGCAG 1 cut(s) 762
BshFI GGCC 5 cut(s) 76, 177, 238, 465, 945
BsiHKAI GWGCWC 1 cut(s) 425
BsiSI CCGG 2 cut(s) 224, 439
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 7 cut(s) 38, 190, 369, 450, 665, 732, 821
BsmAI GTCTC 1 cut(s) 76
BsmBI CGTCTC 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 427
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 5 cut(s) 76, 177, 238, 465, 945
Bsp119I TTCGAA 1 cut(s) 923
Bsp1286I GDGCHC 2 cut(s) 425, 658
Bsp143I GATC 2 cut(s) 676, 996
BspACI CCGC 1 cut(s) 297
BspANI GGCC 5 cut(s) 76, 177, 238, 465, 945
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 2 cut(s) 443, 655
BspOI GCTAGC 1 cut(s) 963
BspQI GCTCTTC 1 cut(s) 858
BspT104I TTCGAA 1 cut(s) 923
BsrDI GCAATG 1 cut(s) 970
BsrI ACTGG 1 cut(s) 742
BssECI CCNNGG 2 cut(s) 188, 946
BssMI GATC 2 cut(s) 676, 996
BssNI GRCGYC 3 cut(s) 85, 287, 670
BssT1I CCWWGG 1 cut(s) 946
Bst2UI CCWGG 5 cut(s) 33, 189, 467, 651, 750
Bst4CI ACNGT 3 cut(s) 792, 854, 941
Bst6I CTCTTC 4 cut(s) 624, 701, 725, 858
BstACI GRCGYC 3 cut(s) 85, 287, 670
BstBI TTCGAA 1 cut(s) 923
BstC8I GCNNGC 3 cut(s) 175, 336, 961
BstDEI CTNAG 2 cut(s) 57, 680
BstF5I GGATG 1 cut(s) 293
BstKTI GATC 2 cut(s) 679, 999
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 2 cut(s) 676, 996
BstNI CCWGG 5 cut(s) 33, 189, 467, 651, 750
BstNSI RCATGY 1 cut(s) 812
BstSCI CCNGG 5 cut(s) 31, 187, 465, 649, 748
BstV1I GCAGC 1 cut(s) 133
BsuRI GGCC 5 cut(s) 76, 177, 238, 465, 945
BtrI CACGTC 1 cut(s) 591
BtsCI GGATG 1 cut(s) 293
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 2 cut(s) 750, 946
Cac8I GCNNGC 3 cut(s) 175, 336, 961
CaiI CAGNNNCTG 1 cut(s) 749
CciI TCATGA 1 cut(s) 699
Cfr13I GGNCC 2 cut(s) 176, 441
CseI GACGC 3 cut(s) 295, 311, 678
Csp6I GTAC 1 cut(s) 855
CviAII CATG 3 cut(s) 700, 809, 838
CviQI GTAC 1 cut(s) 855
DdeI CTNAG 2 cut(s) 57, 680
DpnI GATC 2 cut(s) 678, 998
DpnII GATC 2 cut(s) 676, 996
DraIII CACNNNGTG 1 cut(s) 143
EaeI YGGCCR 2 cut(s) 463, 943
Eam1104I CTCTTC 4 cut(s) 624, 701, 725, 858
EarI CTCTTC 4 cut(s) 624, 701, 725, 858
Ecl136II GAGCTC 1 cut(s) 423
Eco130I CCWWGG 1 cut(s) 946
Eco147I AGGCCT 1 cut(s) 76
Eco24I GRGCYC 2 cut(s) 425, 658
Eco47I GGWCC 1 cut(s) 441
Eco53kI GAGCTC 1 cut(s) 423
EcoICRI GAGCTC 1 cut(s) 423
EcoRII CCWGG 5 cut(s) 31, 187, 465, 649, 748
EcoT14I CCWWGG 1 cut(s) 946
EcoT38I GRGCYC 2 cut(s) 425, 658
ErhI CCWWGG 1 cut(s) 946
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 3 cut(s) 703, 812, 841
FaqI GGGAC 1 cut(s) 427
FatI CATG 3 cut(s) 699, 808, 837
FbaI TGATCA 1 cut(s) 996
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 1 cut(s) 280
FriOI GRGCYC 2 cut(s) 425, 658
Fsp4HI GCNGC 1 cut(s) 147
FspBI CTAG 1 cut(s) 960
GluI GCNGC 1 cut(s) 147
GsuI CTGGAG 1 cut(s) 915
HaeIII GGCC 5 cut(s) 76, 177, 238, 465, 945
HapII CCGG 2 cut(s) 224, 439
HgaI GACGC 3 cut(s) 295, 311, 678
Hin1I GRCGYC 3 cut(s) 85, 287, 670
Hin1II CATG 3 cut(s) 703, 812, 841
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HindIII AAGCTT 1 cut(s) 1003
HinfI GANTC 6 cut(s) 23, 227, 569, 843, 908, 920
HpaII CCGG 2 cut(s) 224, 439
HphI GGTGA 1 cut(s) 213
Hpy166II GTNNAC 1 cut(s) 496
Hpy188I TCNGA 3 cut(s) 782, 1001, 1060
Hpy188III TCNNGA 5 cut(s) 20, 700, 847, 894, 973
Hpy8I GTNNAC 1 cut(s) 496
HpyAV CCTTC 4 cut(s) 324, 406, 606, 631
HpyCH4III ACNGT 3 cut(s) 792, 854, 941
HpyCH4IV ACGT 2 cut(s) 85, 590
HpyCH4V TGCA 6 cut(s) 256, 377, 506, 584, 743, 1041
HpyF3I CTNAG 2 cut(s) 57, 680
HpySE526I ACGT 2 cut(s) 85, 590
Hsp92I GRCGYC 3 cut(s) 85, 287, 670
Hsp92II CATG 3 cut(s) 703, 812, 841
Ksp22I TGATCA 1 cut(s) 996
Kzo9I GATC 2 cut(s) 676, 996
LguI GCTCTTC 1 cut(s) 858
LmnI GCTCC 4 cut(s) 154, 420, 653, 817
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 2 cut(s) 493, 1050
MaeI CTAG 1 cut(s) 960
MaeII ACGT 2 cut(s) 85, 590
MaeIII GTNAC 2 cut(s) 42, 560
MalI GATC 2 cut(s) 678, 998
MboI GATC 2 cut(s) 676, 996
MboII GAAGA 8 cut(s) 205, 395, 621, 641, 688, 742, 875, 882
MhlI GDGCHC 2 cut(s) 425, 658
MlsI TGGCCA 2 cut(s) 465, 945
MluCI AATT 6 cut(s) 270, 274, 510, 624, 711, 828
MluNI TGGCCA 2 cut(s) 465, 945
MlyI GAGTC 3 cut(s) 32, 236, 852
MmeI TCCRAC 3 cut(s) 571, 781, 839
MnlI CCTC 9 cut(s) 300, 312, 373, 444, 726, 853, 890, 898, 910
Mox20I TGGCCA 2 cut(s) 465, 945
MscI TGGCCA 2 cut(s) 465, 945
MseI TTAA 2 cut(s) 273, 474
Msp20I TGGCCA 2 cut(s) 465, 945
MspI CCGG 2 cut(s) 224, 439
MspR9I CCNGG 5 cut(s) 33, 189, 467, 651, 750
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 5 cut(s) 33, 189, 467, 651, 750
NdeII GATC 2 cut(s) 676, 996
NheI GCTAGC 1 cut(s) 959
NlaIII CATG 3 cut(s) 703, 812, 841
NlaIV GGNNCC 2 cut(s) 443, 655
NmuCI GTSAC 2 cut(s) 42, 560
NspI RCATGY 1 cut(s) 812
NspV TTCGAA 1 cut(s) 923
PagI TCATGA 1 cut(s) 699
PceI AGGCCT 1 cut(s) 76
PciSI GCTCTTC 1 cut(s) 858
PctI GAATGC 1 cut(s) 379
PfeI GAWTC 3 cut(s) 569, 908, 920
PkrI GCNGC 1 cut(s) 148
PleI GAGTC 3 cut(s) 31, 235, 851
PpsI GAGTC 3 cut(s) 31, 235, 851
PshBI ATTAAT 2 cut(s) 273, 474
Psp124BI GAGCTC 1 cut(s) 425
Psp6I CCWGG 5 cut(s) 31, 187, 465, 649, 748
PspGI CCWGG 5 cut(s) 31, 187, 465, 649, 748
PspN4I GGNNCC 2 cut(s) 443, 655
PspPI GGNCC 2 cut(s) 176, 441
PstNI CAGNNNCTG 1 cut(s) 749
RsaI GTAC 1 cut(s) 856
RsaNI GTAC 1 cut(s) 855
SacI GAGCTC 1 cut(s) 425
SapI GCTCTTC 1 cut(s) 858
SaqAI TTAA 2 cut(s) 273, 474
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 2 cut(s) 676, 996
Sau96I GGNCC 2 cut(s) 176, 441
SchI GAGTC 3 cut(s) 32, 236, 852
ScrFI CCNGG 5 cut(s) 33, 189, 467, 651, 750
SduI GDGCHC 2 cut(s) 425, 658
SfaNI GCATC 2 cut(s) 493, 1050
SfuI TTCGAA 1 cut(s) 923
SinI GGWCC 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
Sse9I AATT 6 cut(s) 270, 274, 510, 624, 711, 828
SseBI AGGCCT 1 cut(s) 76
SsiI CCGC 1 cut(s) 297
SspMI CTAG 1 cut(s) 960
SstI GAGCTC 1 cut(s) 425
StuI AGGCCT 1 cut(s) 76
StyD4I CCNGG 5 cut(s) 31, 187, 465, 649, 748
StyI CCWWGG 1 cut(s) 946
TaaI ACNGT 3 cut(s) 792, 854, 941
TaiI ACGT 2 cut(s) 88, 593
TaqI TCGA 2 cut(s) 340, 923
TasI AATT 6 cut(s) 270, 274, 510, 624, 711, 828
TatI WGTACW 1 cut(s) 854
TfiI GAWTC 3 cut(s) 569, 908, 920
Tru1I TTAA 2 cut(s) 273, 474
Tru9I TTAA 2 cut(s) 273, 474
TscAI CASTG 2 cut(s) 750, 946
TseFI GTSAC 2 cut(s) 42, 560
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 2 cut(s) 42, 560
TspDTI ATGAA 6 cut(s) 114, 205, 267, 650, 688, 716
TspGWI ACGGA 2 cut(s) 84, 198
TspRI CASTG 2 cut(s) 750, 946
VpaK11BI GGWCC 1 cut(s) 441
VspI ATTAAT 2 cut(s) 273, 474
XceI RCATGY 1 cut(s) 812
XspI CTAG 1 cut(s) 960
ZraI GACGTC 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.