pycom14g10910

gag-polypeptide of LTR copia-type

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0024629
Physical Location & Seq
Forward (+)
13718168 .. 13718677
510 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 510 bp
ATGCTTGTCTTGGCCCTGACCAATTTTCCACCTCTGCCATCCTTTAATGATCTGCGTACCCGTCTCCTAGTCTTTGAGTCCAAGCACGCCATGAAACAGGTTGCTGCATCATCCCATTCTGCTTTCTATTCTGCCCGTCATACGGTTGGTGGTTCGTCTAATGGACGCGGTGGTCACTTTTCTTCTCGCCCCAATGGTGCTTCTGGTGGCAATCAGTTTCCCCATACCCCTAGTGGTGGCCGATCGAATCAACATGGAGGTGTTGGGCGATATGGTCAACGTGGTGCTGTACAACAACGCCTTGGTATTCTTGGACCTGCTCCTGTCAATCGTGGTCCTCAGTGTTGGGCCTGCAACCAATTTGGCCACATTGCTGCATTGTGTCCTCAAGCTACTCGTTCAAATGATGATATCTCTCGTGCCTTTGCTGGTATGCATCTTGCTTCTCCCTCTGATCCAACATGGTATCCCGATACTGGGGCTACTCATCATATGACGAGTGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 Retrotran_gag_3

Protein Analysis

170

Amino Acids

17.89

Weight (kDa)

10.18

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0024629)

Species Orthologous Gene IDs
pyrus_communis pycom04g01430 pycom06g06150 pycom14g10910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 171
Acc36I ACCTGC 1 cut(s) 325
AccII CGCG 1 cut(s) 168
AciI CCGC 1 cut(s) 168
AclWI GGATC 1 cut(s) 449
AcoI YGGCCR 2 cut(s) 238, 364
AfaI GTAC 2 cut(s) 58, 291
AfiI CCNNNNNNNGG 4 cut(s) 142, 236, 476, 477
AgsI TTSAA 1 cut(s) 402
AluBI AGCT 1 cut(s) 392
AluI AGCT 1 cut(s) 392
Alw26I GTCTC 1 cut(s) 68
AlwI GGATC 1 cut(s) 449
AoxI GGCC 4 cut(s) 12, 238, 348, 364
ApeKI GCWGC 2 cut(s) 104, 374
AspS9I GGNCC 4 cut(s) 13, 314, 335, 348
AvaII GGWCC 2 cut(s) 314, 335
BalI TGGCCA 1 cut(s) 366
BauI CACGAG 1 cut(s) 417
BbvI GCAGC 2 cut(s) 91, 361
BccI CCATC 1 cut(s) 46
BciVI GTATCC 1 cut(s) 477
BcoDI GTCTC 1 cut(s) 68
BfaI CTAG 2 cut(s) 68, 231
BfuAI ACCTGC 1 cut(s) 325
BfuI GTATCC 1 cut(s) 477
BisI GCNGC 2 cut(s) 105, 375
BlsI GCNGC 2 cut(s) 106, 376
Bme18I GGWCC 2 cut(s) 314, 335
BmgT120I GGNCC 4 cut(s) 13, 314, 335, 348
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 3 cut(s) 116, 445, 493
BmuI ACTGGG 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 372
BsaJI CCNNGG 1 cut(s) 301
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 236, 476, 477
Bse1I ACTGG 1 cut(s) 481
Bse3DI GCAATG 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 2 cut(s) 38, 110
BseLI CCNNNNNNNGG 4 cut(s) 142, 236, 476, 477
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 481
BseXI GCAGC 2 cut(s) 91, 361
Bsh1236I CGCG 1 cut(s) 168
Bsh1285I CGRYCG 1 cut(s) 245
BshFI GGCC 4 cut(s) 14, 240, 350, 366
BsiEI CGRYCG 1 cut(s) 245
BslI CCNNNNNNNGG 4 cut(s) 142, 236, 476, 477
BsmAI GTCTC 1 cut(s) 68
BsmBI CGTCTC 1 cut(s) 68
BsnI GGCC 4 cut(s) 14, 240, 350, 366
Bsp1407I TGTACA 1 cut(s) 289
Bsp143I GATC 3 cut(s) 49, 242, 454
BspACI CCGC 1 cut(s) 168
BspANI GGCC 4 cut(s) 14, 240, 350, 366
BspCNI CTCAG 1 cut(s) 352
BspFNI CGCG 1 cut(s) 168
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 369
BsrGI TGTACA 1 cut(s) 289
BsrI ACTGG 1 cut(s) 481
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 3 cut(s) 49, 242, 454
BssSI CACGAG 1 cut(s) 417
BssT1I CCWWGG 1 cut(s) 301
Bst2BI CACGAG 1 cut(s) 417
Bst4CI ACNGT 1 cut(s) 145
BstAUI TGTACA 1 cut(s) 289
BstC8I GCNNGC 2 cut(s) 87, 352
BstDEI CTNAG 1 cut(s) 339
BstF5I GGATG 2 cut(s) 38, 110
BstFNI CGCG 1 cut(s) 168
BstKTI GATC 3 cut(s) 52, 245, 457
BstMAI GTCTC 1 cut(s) 68
BstMBI GATC 3 cut(s) 49, 242, 454
BstMCI CGRYCG 1 cut(s) 245
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 91, 361
BsuI GTATCC 1 cut(s) 477
BsuRI GGCC 4 cut(s) 14, 240, 350, 366
BtsCI GGATG 2 cut(s) 38, 110
BtsIMutI CAGTG 1 cut(s) 347
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 2 cut(s) 87, 352
Cfr13I GGNCC 4 cut(s) 13, 314, 335, 348
CseI GACGC 1 cut(s) 174
Csp6I GTAC 2 cut(s) 57, 290
CviAII CATG 3 cut(s) 91, 254, 462
CviJI RGCY 6 cut(s) 14, 240, 350, 366, 392, 482
CviKI_1 RGCY 6 cut(s) 14, 240, 350, 366, 392, 482
CviQI GTAC 2 cut(s) 57, 290
DdeI CTNAG 1 cut(s) 339
DpnI GATC 3 cut(s) 51, 244, 456
DpnII GATC 3 cut(s) 49, 242, 454
DrdI GACNNNNNNGTC 1 cut(s) 171
DseDI GACNNNNNNGTC 1 cut(s) 171
EaeI YGGCCR 2 cut(s) 238, 364
Eco130I CCWWGG 1 cut(s) 301
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 2 cut(s) 314, 335
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 301
EcoT22I ATGCAT 1 cut(s) 438
ErhI CCWWGG 1 cut(s) 301
Esp3I CGTCTC 1 cut(s) 68
FaeI CATG 3 cut(s) 94, 257, 465
FaiI YATR 9 cut(s) 92, 141, 225, 255, 273, 434, 463, 492, 494
FatI CATG 3 cut(s) 90, 253, 461
FauNDI CATATG 1 cut(s) 492
Fnu4HI GCNGC 2 cut(s) 105, 375
FokI GGATG 2 cut(s) 25, 97
Fsp4HI GCNGC 2 cut(s) 105, 375
FspBI CTAG 2 cut(s) 68, 231
GluI GCNGC 2 cut(s) 105, 375
HaeIII GGCC 4 cut(s) 14, 240, 350, 366
HgaI GACGC 1 cut(s) 174
Hin1II CATG 3 cut(s) 94, 257, 465
HincII GTYRAC 1 cut(s) 278
HindII GTYRAC 1 cut(s) 278
HinfI GANTC 2 cut(s) 77, 247
Hpy166II GTNNAC 1 cut(s) 278
Hpy188I TCNGA 1 cut(s) 454
Hpy188III TCNNGA 1 cut(s) 470
Hpy8I GTNNAC 1 cut(s) 278
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 280
HpyCH4V TGCA 4 cut(s) 107, 354, 377, 436
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 1 cut(s) 280
Hsp92II CATG 3 cut(s) 94, 257, 465
Kzo9I GATC 3 cut(s) 49, 242, 454
LmnI GCTCC 1 cut(s) 325
LpnPI CCDG 8 cut(s) 29, 83, 189, 330, 336, 364, 414, 462
Lsp1109I GCAGC 2 cut(s) 91, 361
LweI GCATC 3 cut(s) 116, 445, 493
MaeI CTAG 2 cut(s) 68, 231
MaeII ACGT 1 cut(s) 280
MaeIII GTNAC 1 cut(s) 173
MalI GATC 3 cut(s) 51, 244, 456
MboI GATC 3 cut(s) 49, 242, 454
MboII GAAGA 1 cut(s) 174
MlsI TGGCCA 1 cut(s) 366
MluCI AATT 2 cut(s) 22, 359
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 482
MnlI CCTC 5 cut(s) 42, 251, 348, 396, 460
Mox20I TGGCCA 1 cut(s) 366
Mph1103I ATGCAT 1 cut(s) 438
MscI TGGCCA 1 cut(s) 366
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 1 cut(s) 258
Msp20I TGGCCA 1 cut(s) 366
MvnI CGCG 1 cut(s) 168
NdeI CATATG 1 cut(s) 492
NdeII GATC 3 cut(s) 49, 242, 454
NlaIII CATG 3 cut(s) 94, 257, 465
NmuCI GTSAC 1 cut(s) 173
NsiI ATGCAT 1 cut(s) 438
PfeI GAWTC 1 cut(s) 247
PkrI GCNGC 2 cut(s) 106, 376
Ple19I CGATCG 1 cut(s) 245
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
PspPI GGNCC 4 cut(s) 13, 314, 335, 348
PvuI CGATCG 1 cut(s) 245
RsaI GTAC 2 cut(s) 58, 291
RsaNI GTAC 2 cut(s) 57, 290
RseI CAYNNNNRTG 1 cut(s) 258
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 2 cut(s) 105, 375
Sau3AI GATC 3 cut(s) 49, 242, 454
Sau96I GGNCC 4 cut(s) 13, 314, 335, 348
SchI GAGTC 1 cut(s) 86
SetI ASST 6 cut(s) 34, 102, 262, 283, 319, 394
SfaNI GCATC 3 cut(s) 116, 445, 493
SinI GGWCC 2 cut(s) 314, 335
SmiMI CAYNNNNRTG 1 cut(s) 258
SmlI CTYRAG 1 cut(s) 387
SmoI CTYRAG 1 cut(s) 387
Sse9I AATT 2 cut(s) 22, 359
SsiI CCGC 1 cut(s) 168
SspMI CTAG 2 cut(s) 68, 231
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 1 cut(s) 283
TaqI TCGA 1 cut(s) 245
TasI AATT 2 cut(s) 22, 359
TatI WGTACW 1 cut(s) 289
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 1 cut(s) 347
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 2 cut(s) 104, 374
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 1 cut(s) 107
TspRI CASTG 1 cut(s) 347
VpaK11BI GGWCC 2 cut(s) 314, 335
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XspI CTAG 2 cut(s) 68, 231
Zsp2I ATGCAT 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.