pycom14g20330

Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0029309
Physical Location & Seq
Reverse (-)
21384014 .. 21384607
594 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 423 bp
ATGGCAATGGCCGGTTTTCTGCCTTTCAGTGCTATATACATTGAACTGTATTACATATTTGCAAGTGTATGGGGTCACAGGATTTACACCATTTACAGCATCTTGTTTATTGTGTTTATCACTCTTCTGATCGTCACTGCTTTCATTACCGTGGCATTGACATATTTCCAACTTGCTGCTGAGGACCATGAGTGGTGGTGGAGGTCTTTCCTCTGTGGTGGGTCTACTGGACTATTTATCTATGCCTATTGTCTGTATTACTACTACGCACGCTCGGATATGTCGGGATTCATGCAAACATCATTCTTCTTTGGTTACATGGCATGCATCTGCTACGGTTTCTTCCTCATGCTTGGAACTGTAGGCTTCCGTGCAGCTTTGCTTTTTGTCCGTCACATATACCGGTCGATCAAGTGTGAGTAG

Protein Analysis

141

Amino Acids

16.44

Weight (kDa)

7.62

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 1 - 97 4.5e-39 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029309)

Species Orthologous Gene IDs
pyrus_communis pycom06g20950 pycom14g20330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 9
AgeI ACCGGT 1 cut(s) 402
AgsI TTSAA 1 cut(s) 44
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
AoxI GGCC 1 cut(s) 9
ApeKI GCWGC 2 cut(s) 176, 374
AsiGI ACCGGT 1 cut(s) 402
AspS9I GGNCC 1 cut(s) 184
AvaII GGWCC 1 cut(s) 184
BbvCI CCTCAGC 1 cut(s) 180
BbvI GCAGC 2 cut(s) 163, 386
BfmI CTRYAG 1 cut(s) 360
BisI GCNGC 2 cut(s) 177, 375
BlsI GCNGC 2 cut(s) 178, 376
Bme18I GGWCC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 184
BmsI GCATC 2 cut(s) 108, 336
Bpu10I CCTNAGC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 150
BsaWI WCCGGW 1 cut(s) 402
Bse118I RCCGGY 2 cut(s) 11, 402
Bse1I ACTGG 1 cut(s) 232
Bse3DI GCAATG 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 150
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 232
BseXI GCAGC 2 cut(s) 163, 386
BsgI GTGCAG 1 cut(s) 393
Bsh1285I CGRYCG 1 cut(s) 407
BshFI GGCC 1 cut(s) 11
BshTI ACCGGT 1 cut(s) 402
BsiEI CGRYCG 1 cut(s) 407
BsiSI CCGG 2 cut(s) 12, 403
BsnI GGCC 1 cut(s) 11
Bsp143I GATC 2 cut(s) 129, 408
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 2 cut(s) 11, 402
BsrI ACTGG 1 cut(s) 232
BssAI RCCGGY 2 cut(s) 11, 402
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 2 cut(s) 129, 408
Bst4CI ACNGT 4 cut(s) 48, 151, 338, 361
Bst6I CTCTTC 1 cut(s) 129
BstC8I GCNNGC 2 cut(s) 271, 325
BstDEI CTNAG 1 cut(s) 180
BstDSI CCRYGG 1 cut(s) 150
BstKTI GATC 2 cut(s) 132, 411
BstMBI GATC 2 cut(s) 129, 408
BstMCI CGRYCG 1 cut(s) 407
BstNSI RCATGY 1 cut(s) 327
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 163, 386
BsuRI GGCC 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 2 cut(s) 34, 135
Cac8I GCNNGC 2 cut(s) 271, 325
Cfr10I RCCGGY 2 cut(s) 11, 402
Cfr13I GGNCC 1 cut(s) 184
CspAI ACCGGT 1 cut(s) 402
CviAII CATG 5 cut(s) 188, 292, 319, 324, 349
CviJI RGCY 3 cut(s) 11, 366, 377
CviKI_1 RGCY 3 cut(s) 11, 366, 377
DdeI CTNAG 1 cut(s) 180
DpnI GATC 2 cut(s) 131, 410
DpnII GATC 2 cut(s) 129, 408
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 184
EcoT22I ATGCAT 1 cut(s) 329
FaeI CATG 5 cut(s) 191, 295, 322, 327, 352
FatI CATG 5 cut(s) 187, 291, 318, 323, 348
FblI GTMKAC 1 cut(s) 224
Fnu4HI GCNGC 2 cut(s) 177, 375
Fsp4HI GCNGC 2 cut(s) 177, 375
GluI GCNGC 2 cut(s) 177, 375
HaeIII GGCC 1 cut(s) 11
HapII CCGG 2 cut(s) 12, 403
Hin1II CATG 5 cut(s) 191, 295, 322, 327, 352
HinfI GANTC 1 cut(s) 288
HpaII CCGG 2 cut(s) 12, 403
Hpy166II GTNNAC 1 cut(s) 225
Hpy188I TCNGA 2 cut(s) 129, 277
Hpy188III TCNNGA 1 cut(s) 285
Hpy8I GTNNAC 1 cut(s) 225
HpyCH4III ACNGT 4 cut(s) 48, 151, 338, 361
HpyCH4V TGCA 4 cut(s) 62, 295, 327, 374
HpyF3I CTNAG 1 cut(s) 180
Hsp92II CATG 5 cut(s) 191, 295, 322, 327, 352
Kzo9I GATC 2 cut(s) 129, 408
LpnPI CCDG 4 cut(s) 25, 64, 213, 416
Lsp1109I GCAGC 2 cut(s) 163, 386
LweI GCATC 2 cut(s) 108, 336
MaeIII GTNAC 4 cut(s) 74, 133, 314, 392
MalI GATC 2 cut(s) 131, 410
MboI GATC 2 cut(s) 129, 408
MboII GAAGA 3 cut(s) 116, 298, 334
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 4 cut(s) 175, 195, 221, 356
Mph1103I ATGCAT 1 cut(s) 329
MslI CAYNNNNRTG 1 cut(s) 149
MspI CCGG 2 cut(s) 12, 403
NdeII GATC 2 cut(s) 129, 408
NlaIII CATG 5 cut(s) 191, 295, 322, 327, 352
NmuCI GTSAC 3 cut(s) 74, 133, 392
NsiI ATGCAT 1 cut(s) 329
NspI RCATGY 1 cut(s) 327
PaeI GCATGC 1 cut(s) 327
PfeI GAWTC 1 cut(s) 288
PinAI ACCGGT 1 cut(s) 402
PkrI GCNGC 2 cut(s) 178, 376
PspPI GGNCC 1 cut(s) 184
RseI CAYNNNNRTG 1 cut(s) 149
SatI GCNGC 2 cut(s) 177, 375
Sau3AI GATC 2 cut(s) 129, 408
Sau96I GGNCC 1 cut(s) 184
SetI ASST 2 cut(s) 206, 379
SfaNI GCATC 2 cut(s) 108, 336
SfcI CTRYAG 1 cut(s) 360
SinI GGWCC 1 cut(s) 184
SmiMI CAYNNNNRTG 1 cut(s) 149
SphI GCATGC 1 cut(s) 327
TaaI ACNGT 4 cut(s) 48, 151, 338, 361
TaqI TCGA 1 cut(s) 407
TfiI GAWTC 1 cut(s) 288
TscAI CASTG 2 cut(s) 34, 142
TseFI GTSAC 3 cut(s) 74, 133, 392
TseI GCWGC 2 cut(s) 176, 374
Tsp45I GTSAC 3 cut(s) 74, 133, 392
TspDTI ATGAA 2 cut(s) 133, 280
TspGWI ACGGA 2 cut(s) 359, 380
TspRI CASTG 2 cut(s) 34, 142
VpaK11BI GGWCC 1 cut(s) 184
XceI RCATGY 1 cut(s) 327
XmiI GTMKAC 1 cut(s) 224
Zsp2I ATGCAT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.