pycom14g20390

Belongs to the group II decarboxylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0028329
Physical Location & Seq
Reverse (-)
21438198 .. 21440920
2723 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 933 bp
ATGGTGATCTCAACGGCATCCGGTGAGCGTCGTGGAGAGCAGGTGAACAGTACCTTTGCTTCCAGATATGTGCGTAACGTCCTCCCTAAGTTTCAGATGCCGGAGACGTCGATGCCAAAGGACGTGGCGTATCAGATAATAAACGACGAGCTGATGCTGGACGGGAATCCGCGGCTGAACTTGGCGTCGTTCGTGACGACGTGGATGGAGCCCGAATGCGATCGGCTGATGACGGCTGCCATGAACAAAAACTACGTCGACATGGATGAGTACCCCGTTACTACGGAACTCCAGAATCGTTGTGTGAATATTATCGCACATCTGTTTAATGCTCCCATTGGAGACGGCGAAACTGCTGTTGGTGTGTCAACAGTAGGTTCTTCAGAGGCAATTATGCTGGCAGGCCTAGCTTTCAAAAGGAAGTGGCAGAACAAGAGAAAAGGAGAGGGAAAGCCTTTTGATAAGCCCAACATTGTCACCGGAGCTAATGTGCAGGAGGTGAAGCTGTCGGAGGGATACTACGTGATGGACCCTGCGAAAGCAGTTGAGATGGTTGATGAGAACACTATCTGTGTTGCTGCTATTCTAGGCTCAACCCTAACAGGAGAGTTCGAAGATGTGAAGCTCCTTCATGATCTCCTTGTTGAAAAGAATAAGCAGACGGGCTGGGACACTCCCATTCATGTCGACGCTGCCAGCGGAGGCTTTATTGCTCCGTTTCTGTATCCTGAGCTCGAGTGGGATTTCCGTTTGCCGTTAGTCAAGAGCATCAATGTGAGTGGTCACAAGTATGGCCTTGTATACGCCGGTGTTGGGTGGGTTGTGTGGAGGAGTAAGGAGGACTTGCCGGACGAGCTTATATTTCACATCAATTACCTTGGATCGATCAGCCCACTTTCACCCTCAACTTTTCTAAAGGTTCTAGCCAGATAA

Protein Analysis

311

Amino Acids

34.48

Weight (kDa)

5.18

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 34 - 295 6.9e-69 Pyridoxal-dependent decarboxylase conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028329)

Species Orthologous Gene IDs
malus_domestica MD14G1242700.v1.1
pyrus_communis pycom14g20390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
AatII GACGTC 1 cut(s) 110
Acc36I ACCTGC 1 cut(s) 31
AccI GTMKAC 3 cut(s) 258, 687, 801
AccII CGCG 1 cut(s) 172
AciI CCGC 3 cut(s) 170, 172, 699
AclWI GGATC 1 cut(s) 889
AcuI CTGAAG 1 cut(s) 366
AcyI GRCGYC 2 cut(s) 107, 185
AfaI GTAC 2 cut(s) 52, 272
AfiI CCNNNNNNNGG 1 cut(s) 813
AgsI TTSAA 2 cut(s) 415, 647
AjiI CACGTC 2 cut(s) 124, 201
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AluBI AGCT 7 cut(s) 151, 410, 485, 505, 625, 733, 856
AluI AGCT 7 cut(s) 151, 410, 485, 505, 625, 733, 856
Alw21I GWGCWC 1 cut(s) 735
Alw26I GTCTC 2 cut(s) 98, 336
AlwI GGATC 1 cut(s) 889
Ama87I CYCGRG 1 cut(s) 734
AoxI GGCC 2 cut(s) 403, 793
ApeKI GCWGC 3 cut(s) 236, 578, 692
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
AspS9I GGNCC 1 cut(s) 529
AsuHPI GGTGA 6 cut(s) 16, 35, 55, 469, 511, 891
AsuII TTCGAA 1 cut(s) 612
AvaI CYCGRG 1 cut(s) 734
AvaII GGWCC 1 cut(s) 529
BanII GRGCYC 2 cut(s) 213, 735
BarI GAAGNNNNNNTAC 2 cut(s) 43, 75
Bbv12I GWGCWC 1 cut(s) 735
BbvI GCAGC 3 cut(s) 223, 565, 679
BccI CCATC 3 cut(s) 199, 520, 544
BceAI ACGGC 4 cut(s) 30, 249, 361, 739
BciVI GTATCC 2 cut(s) 509, 735
BcoDI GTCTC 2 cut(s) 98, 336
BfaI CTAG 3 cut(s) 407, 587, 923
BfuAI ACCTGC 1 cut(s) 31
BfuI GTATCC 2 cut(s) 509, 735
BisI GCNGC 4 cut(s) 173, 237, 579, 693
BlsI GCNGC 4 cut(s) 174, 238, 580, 694
Bme18I GGWCC 1 cut(s) 529
BmeT110I CYCGRG 1 cut(s) 734
BmgBI CACGTC 2 cut(s) 124, 201
BmgT120I GGNCC 1 cut(s) 529
BmiI GGNNCC 2 cut(s) 210, 531
BmsI GCATC 5 cut(s) 26, 87, 102, 144, 777
BpmI CTGGAG 1 cut(s) 275
Bpu10I CCTNAGC 1 cut(s) 729
Bpu14I TTCGAA 1 cut(s) 612
Bsa29I ATCGAT 1 cut(s) 884
BsaAI YACGTR 1 cut(s) 523
BsaHI GRCGYC 2 cut(s) 107, 185
BsaJI CCNNGG 2 cut(s) 170, 877
BsaWI WCCGGW 2 cut(s) 20, 479
BsaXI ACNNNNNCTCC 4 cut(s) 27, 57, 474, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 813
Bse118I RCCGGY 1 cut(s) 806
BseCI ATCGAT 1 cut(s) 884
BseDI CCNNGG 2 cut(s) 170, 877
BseGI GGATG 3 cut(s) 17, 210, 271
BseLI CCNNNNNNNGG 1 cut(s) 813
BseMII CTCAG 1 cut(s) 720
BseRI GAGGAG 1 cut(s) 844
BseXI GCAGC 3 cut(s) 223, 565, 679
BseYI CCCAGC 1 cut(s) 666
BsgI GTGCAG 1 cut(s) 512
Bsh1236I CGCG 1 cut(s) 172
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 2 cut(s) 405, 795
BshVI ATCGAT 1 cut(s) 884
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 735
BsiHKCI CYCGRG 1 cut(s) 734
BsiSI CCGG 5 cut(s) 21, 101, 480, 807, 848
BslFI GGGAC 1 cut(s) 683
BslI CCNNNNNNNGG 1 cut(s) 813
BsmAI GTCTC 2 cut(s) 98, 336
BsmBI CGTCTC 2 cut(s) 98, 336
BsmFI GGGAC 1 cut(s) 683
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 2 cut(s) 405, 795
BsoBI CYCGRG 1 cut(s) 734
Bsp119I TTCGAA 1 cut(s) 612
Bsp1286I GDGCHC 2 cut(s) 213, 735
Bsp143I GATC 5 cut(s) 6, 220, 634, 881, 885
BspACI CCGC 3 cut(s) 170, 172, 699
BspANI GGCC 2 cut(s) 405, 795
BspCNI CTCAG 1 cut(s) 721
BspDI ATCGAT 1 cut(s) 884
BspFNI CGCG 1 cut(s) 172
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 2 cut(s) 210, 531
BspMI ACCTGC 1 cut(s) 31
BspPI GGATC 1 cut(s) 889
BspT104I TTCGAA 1 cut(s) 612
BsrFI RCCGGY 1 cut(s) 806
BssAI RCCGGY 1 cut(s) 806
BssECI CCNNGG 2 cut(s) 170, 877
BssMI GATC 5 cut(s) 6, 220, 634, 881, 885
BssNAI GTATAC 1 cut(s) 802
BssNI GRCGYC 2 cut(s) 107, 185
BssT1I CCWWGG 1 cut(s) 877
Bst1107I GTATAC 1 cut(s) 802
Bst4CI ACNGT 2 cut(s) 50, 373
BstACI GRCGYC 2 cut(s) 107, 185
BstBAI YACGTR 1 cut(s) 523
BstBI TTCGAA 1 cut(s) 612
BstC8I GCNNGC 3 cut(s) 399, 403, 697
BstDEI CTNAG 2 cut(s) 87, 729
BstDSI CCRYGG 1 cut(s) 170
BstF5I GGATG 3 cut(s) 17, 210, 271
BstFNI CGCG 1 cut(s) 172
BstKTI GATC 5 cut(s) 9, 223, 637, 884, 888
BstMAI GTCTC 2 cut(s) 98, 336
BstMBI GATC 5 cut(s) 6, 220, 634, 881, 885
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 2 cut(s) 407, 853
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 3 cut(s) 223, 565, 679
BstZ17I GTATAC 1 cut(s) 802
Bsu15I ATCGAT 1 cut(s) 884
BsuI GTATCC 2 cut(s) 509, 735
BsuRI GGCC 2 cut(s) 405, 795
BsuTUI ATCGAT 1 cut(s) 884
BtgI CCRYGG 1 cut(s) 170
BtrI CACGTC 2 cut(s) 124, 201
BtsCI GGATG 3 cut(s) 17, 210, 271
BveI ACCTGC 1 cut(s) 31
Cac8I GCNNGC 3 cut(s) 399, 403, 697
CciI TCATGA 1 cut(s) 631
Cfr10I RCCGGY 1 cut(s) 806
Cfr13I GGNCC 1 cut(s) 529
Cfr42I CCGCGG 1 cut(s) 173
ClaI ATCGAT 1 cut(s) 884
CseI GACGC 3 cut(s) 17, 174, 698
Csp6I GTAC 2 cut(s) 51, 271
CspCI CAANNNNNGTGG 4 cut(s) 105, 140, 760, 795
CviAII CATG 4 cut(s) 241, 262, 632, 683
CviQI GTAC 2 cut(s) 51, 271
DdeI CTNAG 2 cut(s) 87, 729
DpnI GATC 5 cut(s) 8, 222, 636, 883, 887
DpnII GATC 5 cut(s) 6, 220, 634, 881, 885
Ecl136II GAGCTC 1 cut(s) 733
Eco130I CCWWGG 1 cut(s) 877
Eco147I AGGCCT 1 cut(s) 405
Eco24I GRGCYC 2 cut(s) 213, 735
Eco47I GGWCC 1 cut(s) 529
Eco53kI GAGCTC 1 cut(s) 733
Eco57I CTGAAG 1 cut(s) 366
Eco88I CYCGRG 1 cut(s) 734
EcoICRI GAGCTC 1 cut(s) 733
EcoT14I CCWWGG 1 cut(s) 877
EcoT38I GRGCYC 2 cut(s) 213, 735
ErhI CCWWGG 1 cut(s) 877
Esp3I CGTCTC 2 cut(s) 98, 336
FaeI CATG 4 cut(s) 244, 265, 635, 686
FaiI YATR 9 cut(s) 69, 242, 263, 395, 633, 684, 792, 802, 860
FalI AAGNNNNNCTT 2 cut(s) 827, 859
FaqI GGGAC 1 cut(s) 683
FatI CATG 4 cut(s) 240, 261, 631, 682
FblI GTMKAC 3 cut(s) 258, 687, 801
Fnu4HI GCNGC 4 cut(s) 173, 237, 579, 693
FokI GGATG 3 cut(s) 4, 217, 278
FriOI GRGCYC 2 cut(s) 213, 735
Fsp4HI GCNGC 4 cut(s) 173, 237, 579, 693
FspBI CTAG 3 cut(s) 407, 587, 923
GluI GCNGC 4 cut(s) 173, 237, 579, 693
GsaI CCCAGC 1 cut(s) 670
GsuI CTGGAG 1 cut(s) 275
HaeIII GGCC 2 cut(s) 405, 795
HapII CCGG 5 cut(s) 21, 101, 480, 807, 848
HgaI GACGC 3 cut(s) 17, 174, 698
Hin1I GRCGYC 2 cut(s) 107, 185
Hin1II CATG 4 cut(s) 244, 265, 635, 686
HincII GTYRAC 3 cut(s) 259, 369, 688
HindII GTYRAC 3 cut(s) 259, 369, 688
HinfI GANTC 2 cut(s) 166, 295
HpaII CCGG 5 cut(s) 21, 101, 480, 807, 848
HphI GGTGA 6 cut(s) 16, 35, 55, 469, 511, 891
Hpy166II GTNNAC 5 cut(s) 46, 259, 369, 688, 802
Hpy188I TCNGA 4 cut(s) 96, 135, 385, 511
Hpy188III TCNNGA 6 cut(s) 63, 193, 292, 632, 728, 763
Hpy8I GTNNAC 5 cut(s) 46, 259, 369, 688, 802
Hpy99I CGWCG 7 cut(s) 33, 112, 149, 190, 202, 260, 692
HpyAV CCTTC 1 cut(s) 638
HpyCH4III ACNGT 2 cut(s) 50, 373
HpyCH4IV ACGT 6 cut(s) 78, 107, 123, 200, 255, 522
HpyCH4V TGCA 1 cut(s) 493
HpyF10VI GCNNNNNNNGC 2 cut(s) 407, 853
HpyF3I CTNAG 2 cut(s) 87, 729
HpySE526I ACGT 6 cut(s) 78, 107, 123, 200, 255, 522
Hsp92I GRCGYC 2 cut(s) 107, 185
Hsp92II CATG 4 cut(s) 244, 265, 635, 686
KspI CCGCGG 1 cut(s) 173
Kzo9I GATC 5 cut(s) 6, 220, 634, 881, 885
LmnI GCTCC 5 cut(s) 208, 337, 482, 630, 718
Lsp1109I GCAGC 3 cut(s) 223, 565, 679
LweI GCATC 5 cut(s) 26, 87, 102, 144, 777
MaeI CTAG 3 cut(s) 407, 587, 923
MaeII ACGT 6 cut(s) 78, 107, 123, 200, 255, 522
MaeIII GTNAC 5 cut(s) 74, 193, 277, 475, 782
MalI GATC 5 cut(s) 8, 222, 636, 883, 887
MboI GATC 5 cut(s) 6, 220, 634, 881, 885
MboII GAAGA 2 cut(s) 372, 626
MhlI GDGCHC 2 cut(s) 213, 735
MluCI AATT 2 cut(s) 390, 871
MmeI TCCRAC 1 cut(s) 489
MnlI CCTC 9 cut(s) 92, 379, 439, 490, 505, 695, 822, 832, 913
MseI TTAA 1 cut(s) 327
MslI CAYNNNNRTG 2 cut(s) 773, 789
MspA1I CMGCKG 2 cut(s) 172, 699
MspI CCGG 5 cut(s) 21, 101, 480, 807, 848
Mva1269I GAATGC 1 cut(s) 221
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 2 cut(s) 407, 853
NdeII GATC 5 cut(s) 6, 220, 634, 881, 885
NlaIII CATG 4 cut(s) 244, 265, 635, 686
NlaIV GGNNCC 2 cut(s) 210, 531
NmuCI GTSAC 3 cut(s) 193, 475, 782
NspV TTCGAA 1 cut(s) 612
PaeR7I CTCGAG 1 cut(s) 734
PagI TCATGA 1 cut(s) 631
PaqCI CACCTGC 1 cut(s) 31
PceI AGGCCT 1 cut(s) 405
PcsI WCGNNNNNNNCGW 1 cut(s) 194
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 2 cut(s) 166, 295
PkrI GCNGC 4 cut(s) 174, 238, 580, 694
Ple19I CGATCG 1 cut(s) 223
Ppu21I YACGTR 1 cut(s) 523
Psp124BI GAGCTC 1 cut(s) 735
PspFI CCCAGC 1 cut(s) 666
PspN4I GGNNCC 2 cut(s) 210, 531
PspPI GGNCC 1 cut(s) 529
PspXI VCTCGAGB 1 cut(s) 734
PsrI GAACNNNNNNTAC 2 cut(s) 236, 268
PvuI CGATCG 1 cut(s) 223
RsaI GTAC 2 cut(s) 52, 272
RsaNI GTAC 2 cut(s) 51, 271
RseI CAYNNNNRTG 2 cut(s) 773, 789
SacI GAGCTC 1 cut(s) 735
SacII CCGCGG 1 cut(s) 173
SalI GTCGAC 2 cut(s) 257, 686
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 4 cut(s) 173, 237, 579, 693
Sau3AI GATC 5 cut(s) 6, 220, 634, 881, 885
Sau96I GGNCC 1 cut(s) 529
SduI GDGCHC 2 cut(s) 213, 735
SfaNI GCATC 5 cut(s) 26, 87, 102, 144, 777
Sfr274I CTCGAG 1 cut(s) 734
Sfr303I CCGCGG 1 cut(s) 173
SfuI TTCGAA 1 cut(s) 612
SgrAI CRCCGGYG 1 cut(s) 806
SgrBI CCGCGG 1 cut(s) 173
SinI GGWCC 1 cut(s) 529
SlaI CTCGAG 1 cut(s) 734
SmiMI CAYNNNNRTG 2 cut(s) 773, 789
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
Sse9I AATT 2 cut(s) 390, 871
SseBI AGGCCT 1 cut(s) 405
SsiI CCGC 3 cut(s) 170, 172, 699
SspI AATATT 1 cut(s) 310
SspMI CTAG 3 cut(s) 407, 587, 923
SstI GAGCTC 1 cut(s) 735
StuI AGGCCT 1 cut(s) 405
StyI CCWWGG 1 cut(s) 877
TaaI ACNGT 2 cut(s) 50, 373
TaiI ACGT 6 cut(s) 81, 110, 126, 203, 258, 525
TaqI TCGA 6 cut(s) 110, 258, 612, 687, 735, 884
TasI AATT 2 cut(s) 390, 871
TauI GCSGC 1 cut(s) 175
TfiI GAWTC 2 cut(s) 166, 295
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TseFI GTSAC 3 cut(s) 193, 475, 782
TseI GCWGC 3 cut(s) 236, 578, 692
Tsp45I GTSAC 3 cut(s) 193, 475, 782
TspDTI ATGAA 3 cut(s) 257, 620, 671
TspGWI ACGGA 3 cut(s) 299, 705, 737
VpaK11BI GGWCC 1 cut(s) 529
XhoI CTCGAG 1 cut(s) 734
XmiI GTMKAC 3 cut(s) 258, 687, 801
XspI CTAG 3 cut(s) 407, 587, 923
ZraI GACGTC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.