pycom15g08330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0022338
Physical Location & Seq
Reverse (-)
5223025 .. 5223859
835 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
ATGACAGGTCCGATCCGAATCAGAGTTTTCCAACTCCAGCTCGTGTCCCTCAGATTTTTGGCTAAAAGCAAGCAACCGTCTGATTTGACGACGCAGGCTTACTCCTTAGCCAGGGGCTCCTGGAAGACTCTTAGTCCTTCTCCTCCTACTGTTTCTATTCCTGGTTCCGATCTTCTGCATATAACGGTGCATCGTAAGTCTGTTTTCAATGATGCCGTGCAATGGTTTCTATGCCGCAATCTAAACTACAAAGAACTTGGCATTGTGTCGTTTGATATGGCCACTGAATCGTTCAGCCAGATCGAGTTACCGAAACCTTTGAGAAAAACGACAGACAGCCTTGTTCTTTCGGTACACGAGGATTCCCTCGCCTTGATCAAGATTTTCCAGCGCCGTTACTCAAGATCAAGAGTAATTACTCATTGTTATCTTAAGTTATGGGTGATGCAACAGTATGGTGCGGTGAAATCGTGGACTGAATCATACAGCGTGACGCTCTCGAAGCCAGTCTCTTCAGTCTACCATGTTGGTTTTATAAGTAACGGCGAACAAGTGTTCAAAAGCACAAAAGATATCAGACTTGTAATGGTGGATTTTAAGACCAAACAAGTTAGGTATTCTGGAGTTGGAGAGGGACGGAATTACGAGTCCATGGATTCTTTTGTAGAGAGCCTTCTGTTACTTGACCAATCCAATGCTTGTTCTTACCAAGTAGCAGGAACAAGCAAACCAACAAGTCTGGTGGATTGTAGTTGGCACTTTCTTTTCACTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

259

Amino Acids

29.3

Weight (kDa)

9.41

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 57 - 209 8.5e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022338)

Species Orthologous Gene IDs
malus_domestica MD08G1108400.v1.1 MD15G1089700.v1.1
pyrus_communis pycom08g09030 pycom15g08330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 536
AccI GTMKAC 1 cut(s) 519
AciI CCGC 2 cut(s) 235, 461
AclWI GGATC 1 cut(s) 7
AcoI YGGCCR 1 cut(s) 279
AcuI CTGAAG 1 cut(s) 498
AfaI GTAC 1 cut(s) 354
AfiI CCNNNNNNNGG 2 cut(s) 111, 222
AflII CTTAAG 1 cut(s) 431
AgsI TTSAA 2 cut(s) 208, 559
AhdI GACNNNNNGTC 1 cut(s) 132
AjnI CCWGG 3 cut(s) 110, 119, 160
AluBI AGCT 1 cut(s) 40
AluI AGCT 1 cut(s) 40
Alw26I GTCTC 1 cut(s) 514
AlwI GGATC 1 cut(s) 7
AoxI GGCC 1 cut(s) 279
AspLEI GCGC 1 cut(s) 393
AspS9I GGNCC 1 cut(s) 8
AsuHPI GGTGA 2 cut(s) 454, 475
AvaII GGWCC 1 cut(s) 8
BalI TGGCCA 1 cut(s) 281
BanII GRGCYC 1 cut(s) 119
BarI GAAGNNNNNNTAC 2 cut(s) 657, 689
BauI CACGAG 2 cut(s) 41, 356
BbsI GAAGAC 1 cut(s) 131
BceAI ACGGC 3 cut(s) 200, 378, 559
BciT130I CCWGG 3 cut(s) 112, 121, 162
BclI TGATCA 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 514
BfoI RGCGCY 1 cut(s) 394
BfrI CTTAAG 1 cut(s) 431
BisI GCNGC 1 cut(s) 235
BlsI GCNGC 1 cut(s) 236
Bme1390I CCNGG 3 cut(s) 112, 121, 162
Bme18I GGWCC 1 cut(s) 8
BmeRI GACNNNNNGTC 1 cut(s) 132
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 2 cut(s) 118, 166
BmrFI CCNGG 3 cut(s) 112, 121, 162
BmsI GCATC 3 cut(s) 199, 202, 435
BpiI GAAGAC 1 cut(s) 131
BpmI CTGGAG 2 cut(s) 20, 642
Bpu10I CCTNAGC 1 cut(s) 106
BpuEI CTTGAG 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 17
BsaJI CCNNGG 2 cut(s) 111, 651
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 222
Bse1I ACTGG 1 cut(s) 506
Bse3DI GCAATG 1 cut(s) 227
Bse8I GATNNNNATC 1 cut(s) 17
BseBI CCWGG 3 cut(s) 112, 121, 162
BseDI CCNNGG 2 cut(s) 111, 651
BseJI GATNNNNATC 1 cut(s) 17
BseLI CCNNNNNNNGG 2 cut(s) 111, 222
BseMI GCAATG 1 cut(s) 227
BseMII CTCAG 1 cut(s) 64
BseNI ACTGG 1 cut(s) 506
BseRI GAGGAG 1 cut(s) 132
BshFI GGCC 1 cut(s) 281
BslFI GGGAC 2 cut(s) 31, 648
BslI CCNNNNNNNGG 2 cut(s) 111, 222
BsmAI GTCTC 1 cut(s) 514
BsmFI GGGAC 2 cut(s) 31, 648
BsnI GGCC 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 119
Bsp143I GATC 5 cut(s) 12, 169, 300, 375, 404
Bsp19I CCATGG 1 cut(s) 651
BspACI CCGC 2 cut(s) 235, 461
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 1 cut(s) 63
BspLI GGNNCC 2 cut(s) 118, 166
BspPI GGATC 1 cut(s) 7
BspTI CTTAAG 1 cut(s) 431
BsrDI GCAATG 1 cut(s) 227
BsrI ACTGG 1 cut(s) 506
BssECI CCNNGG 2 cut(s) 111, 651
BssMI GATC 5 cut(s) 12, 169, 300, 375, 404
BssSI CACGAG 2 cut(s) 41, 356
BssT1I CCWWGG 1 cut(s) 651
Bst2BI CACGAG 2 cut(s) 41, 356
Bst2UI CCWGG 3 cut(s) 112, 121, 162
Bst4CI ACNGT 4 cut(s) 78, 151, 187, 453
Bst6I CTCTTC 1 cut(s) 517
BstAFI CTTAAG 1 cut(s) 431
BstC8I GCNNGC 2 cut(s) 71, 96
BstDEI CTNAG 3 cut(s) 50, 106, 131
BstDSI CCRYGG 1 cut(s) 651
BstENI CCTNNNNNAGG 1 cut(s) 109
BstH2I RGCGCY 1 cut(s) 394
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 5 cut(s) 15, 172, 303, 378, 407
BstMAI GTCTC 1 cut(s) 514
BstMBI GATC 5 cut(s) 12, 169, 300, 375, 404
BstMWI GCNNNNNNNGC 1 cut(s) 502
BstNI CCWGG 3 cut(s) 112, 121, 162
BstSCI CCNGG 3 cut(s) 110, 119, 160
BstV2I GAAGAC 1 cut(s) 131
BsuRI GGCC 1 cut(s) 281
BtgI CCRYGG 1 cut(s) 651
BtsIMutI CAGTG 1 cut(s) 282
Cac8I GCNNGC 2 cut(s) 71, 96
CfoI GCGC 1 cut(s) 393
Cfr13I GGNCC 1 cut(s) 8
CseI GACGC 2 cut(s) 100, 502
Csp6I GTAC 1 cut(s) 353
CspCI CAANNNNNGTGG 2 cut(s) 723, 758
CviAII CATG 2 cut(s) 524, 652
CviQI GTAC 1 cut(s) 353
DdeI CTNAG 3 cut(s) 50, 106, 131
DpnI GATC 5 cut(s) 14, 171, 302, 377, 406
DpnII GATC 5 cut(s) 12, 169, 300, 375, 404
DriI GACNNNNNGTC 1 cut(s) 132
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 517
Eam1105I GACNNNNNGTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 517
Eco130I CCWWGG 1 cut(s) 651
Eco24I GRGCYC 1 cut(s) 119
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 498
EcoNI CCTNNNNNAGG 1 cut(s) 109
EcoRII CCWGG 3 cut(s) 110, 119, 160
EcoRV GATATC 1 cut(s) 574
EcoT14I CCWWGG 1 cut(s) 651
EcoT38I GRGCYC 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 651
FaeI CATG 2 cut(s) 527, 655
FaqI GGGAC 2 cut(s) 31, 648
FatI CATG 2 cut(s) 523, 651
FbaI TGATCA 1 cut(s) 375
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 1 cut(s) 235
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 1 cut(s) 235
GlaI GCGC 1 cut(s) 392
GluI GCNGC 1 cut(s) 235
GsuI CTGGAG 2 cut(s) 20, 642
HaeII RGCGCY 1 cut(s) 394
HaeIII GGCC 1 cut(s) 281
HgaI GACGC 2 cut(s) 100, 502
HhaI GCGC 1 cut(s) 393
Hin1II CATG 2 cut(s) 527, 655
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HinfI GANTC 7 cut(s) 18, 127, 287, 362, 479, 647, 656
HphI GGTGA 2 cut(s) 454, 475
Hpy166II GTNNAC 3 cut(s) 355, 474, 520
Hpy188I TCNGA 7 cut(s) 12, 17, 23, 53, 82, 169, 578
Hpy188III TCNNGA 5 cut(s) 379, 402, 408, 499, 621
Hpy8I GTNNAC 3 cut(s) 355, 474, 520
Hpy99I CGWCG 1 cut(s) 94
HpyAV CCTTC 2 cut(s) 147, 683
HpyCH4III ACNGT 4 cut(s) 78, 151, 187, 453
HpyCH4V TGCA 4 cut(s) 178, 190, 220, 448
HpyF10VI GCNNNNNNNGC 1 cut(s) 502
HpyF3I CTNAG 3 cut(s) 50, 106, 131
Hsp92II CATG 2 cut(s) 527, 655
HspAI GCGC 1 cut(s) 391
Ksp22I TGATCA 1 cut(s) 375
Kzo9I GATC 5 cut(s) 12, 169, 300, 375, 404
LmnI GCTCC 1 cut(s) 122
LweI GCATC 3 cut(s) 199, 202, 435
MaeIII GTNAC 5 cut(s) 306, 395, 490, 539, 678
MalI GATC 5 cut(s) 14, 171, 302, 377, 406
MboI GATC 5 cut(s) 12, 169, 300, 375, 404
MboII GAAGA 3 cut(s) 136, 164, 504
MhlI GDGCHC 1 cut(s) 119
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 2 cut(s) 414, 640
MluNI TGGCCA 1 cut(s) 281
MlyI GAGTC 2 cut(s) 121, 656
MmeI TCCRAC 2 cut(s) 55, 607
MnlI CCTC 5 cut(s) 59, 153, 352, 377, 625
Mox20I TGGCCA 1 cut(s) 281
MscI TGGCCA 1 cut(s) 281
MseI TTAA 3 cut(s) 432, 597, 775
Msp20I TGGCCA 1 cut(s) 281
MspCI CTTAAG 1 cut(s) 431
MspR9I CCNGG 3 cut(s) 112, 121, 162
MvaI CCWGG 3 cut(s) 112, 121, 162
MwoI GCNNNNNNNGC 1 cut(s) 502
NcoI CCATGG 1 cut(s) 651
NdeII GATC 5 cut(s) 12, 169, 300, 375, 404
NlaIII CATG 2 cut(s) 527, 655
NlaIV GGNNCC 2 cut(s) 118, 166
NmuCI GTSAC 1 cut(s) 490
PfeI GAWTC 5 cut(s) 18, 287, 362, 479, 656
PfoI TCCNGGA 1 cut(s) 119
PkrI GCNGC 1 cut(s) 236
PleI GAGTC 2 cut(s) 121, 655
PpsI GAGTC 2 cut(s) 121, 655
PsiI TTATAA 1 cut(s) 536
Psp6I CCWGG 3 cut(s) 110, 119, 160
PspGI CCWGG 3 cut(s) 110, 119, 160
PspN4I GGNNCC 2 cut(s) 118, 166
PspPI GGNCC 1 cut(s) 8
RsaI GTAC 1 cut(s) 354
RsaNI GTAC 1 cut(s) 353
SaqAI TTAA 3 cut(s) 432, 597, 775
SatI GCNGC 1 cut(s) 235
Sau3AI GATC 5 cut(s) 12, 169, 300, 375, 404
Sau96I GGNCC 1 cut(s) 8
SchI GAGTC 2 cut(s) 121, 656
ScrFI CCNGG 3 cut(s) 112, 121, 162
SduI GDGCHC 1 cut(s) 119
SetI ASST 4 cut(s) 10, 42, 319, 617
SfaNI GCATC 3 cut(s) 199, 202, 435
SinI GGWCC 1 cut(s) 8
SmlI CTYRAG 2 cut(s) 400, 431
SmoI CTYRAG 2 cut(s) 400, 431
Sse9I AATT 2 cut(s) 414, 640
SsiI CCGC 2 cut(s) 235, 461
StyD4I CCNGG 3 cut(s) 110, 119, 160
StyI CCWWGG 1 cut(s) 651
TaaI ACNGT 4 cut(s) 78, 151, 187, 453
TaqI TCGA 2 cut(s) 303, 500
TasI AATT 2 cut(s) 414, 640
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 5 cut(s) 18, 287, 362, 479, 656
Tru1I TTAA 3 cut(s) 432, 597, 775
Tru9I TTAA 3 cut(s) 432, 597, 775
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 1 cut(s) 490
Tsp45I GTSAC 1 cut(s) 490
TspGWI ACGGA 1 cut(s) 652
TspRI CASTG 1 cut(s) 289
Vha464I CTTAAG 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 8
XagI CCTNNNNNAGG 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.