pycom15g11810

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0021140
Physical Location & Seq
Reverse (-)
7990667 .. 7992250
1584 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1584 bp
ATGAAATCTCAGGTCCCCAAATCCCTCCTAACTCTCATCGAGACCTGCACAGGCATTCAACAACTGAAGCAGATCCACGCCAAATCCCTAATCTCGGGCCTTTCCTACAACCAATTCATCCTCAGCCAGATCACCAATTCCTTCCTGCTCCCTCAATCTCTGAACTACGCCACCCGCTTATTGATTCGAACTCAAGAACCAAGCGTTTTCGTCTACAATTCCATGATCCGAGCACATTCCCAATCAGAAACCCCATTTGTTGCTCTGTCGATTTATAACATGATGAGGGTGCAAGAAAATGCTTTGGGCGACAGGTTTACTTATCCTTTTGTGTTGAAGGCTTGCGCTAGCGAATCGGATATGGACAAAGGGAGGGAGGTTCATGGTGTAGTTGTGAGAATCGGGTTTGACTTCGACAGGTATTTGTGTACTTCTTTGCTGAACTTTTATGGGGTTTGTGGAAAAATTGAGGGTGCAAGGCAGGTGTTTGATGAATTTACGGTGAAGGATGTTGTTTTCTGGAATTCCATGATAATGGGTTATGCTCGAAACGGGATGGTTTTTGAAGCTTGTGAGGTTTTTAGGGTGATGGTGGAAGTGGGAGAGGTTAGACCCAATGAAAGTGCGGTTTTGGCATTGATTTCGGCTTGCACGGTTTCGAAAAACTGGAAATTTGGAAGAGAGATTCATGGGTTTTTGAGAAAAGAGGTGACTTTTGGGTCAAATGTGAAAGTTGGAGCTGCACTTGTTGATTTGTATGCAAAATGTGGGTGTTTGGATTATGCAAAGAGAGTGTTTGAGGGAATGCCTGAGAAGAATGCTGTGGTATGGAACTCTTTGATAAGTGGCTATTCCCTCAACGGGTTGTCGAAAGAAGCAATTGATGTTTTCAGGGAAATGTGTTGTTTGGATGTTAAGCCCGATACGTTCACGATTTCAGGCTTGTTGTCCGCTTGTGCTCAAATGGGTGCTGTTAATATAGGAAACTGGGTTCGGAAATTCGCAGAGAAGAATGGACTTTGGGATGTGTTTATAGGTACATCTTTGGTAGACCTGTATGCAAAATGTGGTTCCATTGAAGCAGCTAGAGACGTGTTTGATCAAATGGATAAGAAAACTGTTGCAACATGGAACGCTATTCTCTCTGGATATGCGTCAAATGGGCAGGCTGGATCTGCAATAGAGCTCTTTGATGATATGAGAAAATCAGGTGTCATGCCAGATAGTGTAACGTTCCTTTCGATTTTACATGCTTGTGCACATGCTGGTTTGGTTGAAAAAGGTAAACAATACTTTGATTTGATGGTGAAAACTTATAAGATTGCCCCAAAAGTTGAGCATTACGGTTGCATGGTTGACCTTCTTGGGCGCGCTGGACTTTTGAAAGAAGCAAGGAAGCTTATTGAGAGGATGGATATTGAACCAAATGTAGTTGTATGGGGATCACTGTTTAATGCTTGTAGCATTCATGGTGATACTGAGTTAGGAGAGTGGGTGGCTCGATCATGTTTTCAACTTAGAAGCAATGGATGGGGGCTCCTACGTGCTGTTGGCAAATATGTACGCTGCAGCACGAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

528

Amino Acids

58.66

Weight (kDa)

8.34

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 169 - 217 1e-06 PPR repeat family
PPR_1 PF12854 170 - 198 1.7e-06 PPR repeat
PPR PF01535 172 - 201 5.9e-08 PPR repeat
PPR_2 PF13041 273 - 320 8.9e-12 PPR repeat family
PPR PF01535 275 - 302 1.1e-07 PPR repeat
PPR_2 PF13041 373 - 421 2e-14 PPR repeat family
PPR_3 PF13812 374 - 419 5.2e-06 Pentatricopeptide repeat domain
PPR PF01535 376 - 405 1.4e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021140)

Species Orthologous Gene IDs
malus_domestica MD15G1131000.v1.1
prunus_persica Prupe.1G486300_v2.0.a1 Prupe.1G486300_v2.0.a1 Prupe.1G486300_v2.0.a1
pyrus_communis pycom15g11810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 276, 1317
AarI CACCTGC 1 cut(s) 472
AasI GACNNNNNNGTC 1 cut(s) 718
Acc36I ACCTGC 2 cut(s) 53, 472
AccI GTMKAC 2 cut(s) 213, 1050
AccII CGCG 1 cut(s) 1371
AciI CCGC 3 cut(s) 175, 626, 951
AclI AACGTT 1 cut(s) 1232
AclWI GGATC 4 cut(s) 67, 220, 1180, 1450
AcsI RAATTY 4 cut(s) 494, 523, 671, 998
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 3 cut(s) 430, 1039, 1563
AfiI CCNNNNNNNGG 2 cut(s) 94, 861
AflIII ACRYGT 1 cut(s) 1092
AgsI TTSAA 8 cut(s) 59, 337, 566, 1079, 1277, 1384, 1421, 1514
AjiI CACGTC 1 cut(s) 1093
AloI GAACNNNNNNTCC 2 cut(s) 975, 1007
AluBI AGCT 5 cut(s) 569, 740, 1085, 1186, 1399
AluI AGCT 5 cut(s) 569, 740, 1085, 1186, 1399
Alw21I GWGCWC 4 cut(s) 235, 961, 1188, 1261
Alw26I GTCTC 2 cut(s) 35, 1083
Alw44I GTGCAC 1 cut(s) 1257
AlwI GGATC 4 cut(s) 67, 220, 1180, 1450
Ama87I CYCGRG 1 cut(s) 94
AoxI GGCC 1 cut(s) 97
ApaLI GTGCAC 1 cut(s) 1257
ApeKI GCWGC 4 cut(s) 740, 1082, 1566, 1569
ApoI RAATTY 4 cut(s) 494, 523, 671, 998
AspLEI GCGC 3 cut(s) 347, 1371, 1373
AspS9I GGNCC 2 cut(s) 13, 97
AsuHPI GGTGA 6 cut(s) 124, 514, 598, 721, 1318, 1484
AsuII TTCGAA 2 cut(s) 187, 659
AsuNHI GCTAGC 1 cut(s) 347
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 1 cut(s) 13
BaeGI GKGCMC 1 cut(s) 1261
BanII GRGCYC 2 cut(s) 1188, 1539
Bbv12I GWGCWC 4 cut(s) 235, 961, 1188, 1261
BbvCI CCTCAGC 1 cut(s) 122
BbvI GCAGC 3 cut(s) 727, 1094, 1553
BccI CCATC 5 cut(s) 550, 583, 1297, 1405, 1524
BcgI CGANNNNNNTGC 2 cut(s) 624, 658
BclI TGATCA 1 cut(s) 1099
BcoDI GTCTC 2 cut(s) 35, 1083
BfaI CTAG 2 cut(s) 348, 1086
BfmI CTRYAG 1 cut(s) 1567
BfuAI ACCTGC 2 cut(s) 53, 472
BisI GCNGC 4 cut(s) 741, 1083, 1567, 1570
BlsI GCNGC 4 cut(s) 742, 1084, 1568, 1571
Bme18I GGWCC 1 cut(s) 13
BmeT110I CYCGRG 1 cut(s) 94
BmgBI CACGTC 1 cut(s) 1093
BmgT120I GGNCC 2 cut(s) 13, 97
BmiI GGNNCC 3 cut(s) 15, 1072, 1538
BmrI ACTGGG 1 cut(s) 997
BmtI GCTAGC 1 cut(s) 351
BmuI ACTGGG 1 cut(s) 997
Bpu10I CCTNAGC 1 cut(s) 122
Bpu14I TTCGAA 2 cut(s) 187, 659
BpuEI CTTGAG 1 cut(s) 177
BsaAI YACGTR 1 cut(s) 1544
BsaI GGTCTC 1 cut(s) 35
BsaXI ACNNNNNCTCC 2 cut(s) 1479, 1509
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 861
Bse1I ACTGG 2 cut(s) 671, 992
Bse3DI GCAATG 1 cut(s) 1531
BseGI GGATG 7 cut(s) 117, 514, 561, 916, 1030, 1416, 1535
BseLI CCNNNNNNNGG 2 cut(s) 94, 861
BseMI GCAATG 1 cut(s) 1531
BseMII CTCAG 4 cut(s) 23, 136, 801, 1470
BseNI ACTGG 2 cut(s) 671, 992
BsePI GCGCGC 1 cut(s) 1369
BseSI GKGCMC 1 cut(s) 1261
BseXI GCAGC 3 cut(s) 727, 1094, 1553
BsgI GTGCAG 2 cut(s) 31, 726
Bsh1236I CGCG 1 cut(s) 1371
BshFI GGCC 1 cut(s) 99
BsiHKAI GWGCWC 4 cut(s) 235, 961, 1188, 1261
BsiHKCI CYCGRG 1 cut(s) 94
BslI CCNNNNNNNGG 2 cut(s) 94, 861
BsmAI GTCTC 2 cut(s) 35, 1083
BsmBI CGTCTC 1 cut(s) 1083
BsmI GAATGC 4 cut(s) 54, 810, 823, 1464
BsnI GGCC 1 cut(s) 99
Bso31I GGTCTC 1 cut(s) 35
BsoBI CYCGRG 1 cut(s) 94
Bsp119I TTCGAA 2 cut(s) 187, 659
Bsp1286I GDGCHC 5 cut(s) 235, 961, 1188, 1261, 1539
Bsp143I GATC 7 cut(s) 72, 129, 225, 1099, 1172, 1442, 1502
BspACI CCGC 3 cut(s) 175, 626, 951
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 4 cut(s) 22, 135, 802, 1471
BspFNI CGCG 1 cut(s) 1371
BspLI GGNNCC 3 cut(s) 15, 1072, 1538
BspMAI CTGCAG 1 cut(s) 1571
BspMI ACCTGC 2 cut(s) 53, 472
BspOI GCTAGC 1 cut(s) 351
BspPI GGATC 4 cut(s) 67, 220, 1180, 1450
BspT104I TTCGAA 2 cut(s) 187, 659
BspTNI GGTCTC 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 1531
BsrI ACTGG 2 cut(s) 671, 992
BssHII GCGCGC 1 cut(s) 1369
BssMI GATC 7 cut(s) 72, 129, 225, 1099, 1172, 1442, 1502
Bst4CI ACNGT 5 cut(s) 502, 655, 1120, 1346, 1449
Bst6I CTCTTC 1 cut(s) 673
BstBAI YACGTR 1 cut(s) 1544
BstBI TTCGAA 2 cut(s) 187, 659
BstC8I GCNNGC 5 cut(s) 343, 349, 649, 1167, 1371
BstDEI CTNAG 5 cut(s) 9, 122, 810, 1479, 1517
BstF5I GGATG 7 cut(s) 117, 514, 561, 916, 1030, 1416, 1535
BstFNI CGCG 1 cut(s) 1371
BstHHI GCGC 3 cut(s) 347, 1371, 1373
BstKTI GATC 7 cut(s) 75, 132, 228, 1102, 1175, 1445, 1505
BstMAI GTCTC 2 cut(s) 35, 1083
BstMBI GATC 7 cut(s) 72, 129, 225, 1099, 1172, 1442, 1502
BstMWI GCNNNNNNNGC 2 cut(s) 632, 1175
BstNSI RCATGY 2 cut(s) 1253, 1265
BstSFI CTRYAG 1 cut(s) 1567
BstSLI GKGCMC 1 cut(s) 1261
BstUI CGCG 1 cut(s) 1371
BstV1I GCAGC 3 cut(s) 727, 1094, 1553
BstX2I RGATCY 2 cut(s) 72, 1172
BstXI CCANNNNNNTGG 1 cut(s) 535
BstYI RGATCY 2 cut(s) 72, 1172
BsuRI GGCC 1 cut(s) 99
BtrI CACGTC 1 cut(s) 1093
BtsCI GGATG 7 cut(s) 117, 514, 561, 916, 1030, 1416, 1535
BtsIMutI CAGTG 1 cut(s) 1445
BveI ACCTGC 2 cut(s) 53, 472
Cac8I GCNNGC 5 cut(s) 343, 349, 649, 1167, 1371
CfoI GCGC 3 cut(s) 347, 1371, 1373
Cfr13I GGNCC 2 cut(s) 13, 97
CseI GACGC 1 cut(s) 1143
Csp6I GTAC 3 cut(s) 429, 1038, 1562
CviQI GTAC 3 cut(s) 429, 1038, 1562
DdeI CTNAG 5 cut(s) 9, 122, 810, 1479, 1517
DpnI GATC 7 cut(s) 74, 131, 227, 1101, 1174, 1444, 1504
DpnII GATC 7 cut(s) 72, 129, 225, 1099, 1172, 1442, 1502
DrdI GACNNNNNNGTC 1 cut(s) 718
DseDI GACNNNNNNGTC 1 cut(s) 718
Eam1104I CTCTTC 1 cut(s) 673
EarI CTCTTC 1 cut(s) 673
Ecl136II GAGCTC 1 cut(s) 1186
Eco24I GRGCYC 2 cut(s) 1188, 1539
Eco31I GGTCTC 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 13
Eco53kI GAGCTC 1 cut(s) 1186
Eco57I CTGAAG 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 1186
EcoO109I RGGNCCY 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 523
EcoT38I GRGCYC 2 cut(s) 1188, 1539
Esp3I CGTCTC 1 cut(s) 1083
FauI CCCGC 1 cut(s) 182
FbaI TGATCA 1 cut(s) 1099
FblI GTMKAC 2 cut(s) 213, 1050
Fnu4HI GCNGC 4 cut(s) 741, 1083, 1567, 1570
FokI GGATG 7 cut(s) 104, 521, 568, 923, 1037, 1423, 1542
FriOI GRGCYC 2 cut(s) 1188, 1539
Fsp4HI GCNGC 4 cut(s) 741, 1083, 1567, 1570
FspBI CTAG 2 cut(s) 348, 1086
GlaI GCGC 3 cut(s) 346, 1370, 1372
GluI GCNGC 4 cut(s) 741, 1083, 1567, 1570
HaeIII GGCC 1 cut(s) 99
HgaI GACGC 1 cut(s) 1143
HhaI GCGC 3 cut(s) 347, 1371, 1373
Hin6I GCGC 3 cut(s) 345, 1369, 1371
HinP1I GCGC 3 cut(s) 345, 1369, 1371
HincII GTYRAC 1 cut(s) 1357
HindII GTYRAC 1 cut(s) 1357
HindIII AAGCTT 2 cut(s) 567, 1397
HinfI GANTC 4 cut(s) 184, 353, 399, 685
HphI GGTGA 6 cut(s) 124, 514, 598, 721, 1318, 1484
Hpy166II GTNNAC 8 cut(s) 214, 318, 429, 930, 1051, 1259, 1286, 1357
Hpy188I TCNGA 5 cut(s) 162, 230, 247, 358, 996
Hpy188III TCNNGA 5 cut(s) 40, 194, 520, 931, 1146
Hpy8I GTNNAC 8 cut(s) 214, 318, 429, 930, 1051, 1259, 1286, 1357
HpyAV CCTTC 5 cut(s) 151, 331, 499, 1370, 1570
HpyCH4III ACNGT 5 cut(s) 502, 655, 1120, 1346, 1449
HpyCH4IV ACGT 4 cut(s) 926, 1092, 1232, 1543
HpyF10VI GCNNNNNNNGC 2 cut(s) 632, 1175
HpyF3I CTNAG 5 cut(s) 9, 122, 810, 1479, 1517
HpySE526I ACGT 4 cut(s) 926, 1092, 1232, 1543
HspAI GCGC 3 cut(s) 345, 1369, 1371
Ksp22I TGATCA 1 cut(s) 1099
Kzo9I GATC 7 cut(s) 72, 129, 225, 1099, 1172, 1442, 1502
LmnI GCTCC 3 cut(s) 153, 737, 1542
Lsp1109I GCAGC 3 cut(s) 727, 1094, 1553
MaeI CTAG 2 cut(s) 348, 1086
MaeII ACGT 4 cut(s) 926, 1092, 1232, 1543
MaeIII GTNAC 2 cut(s) 709, 1228
MalI GATC 7 cut(s) 74, 131, 227, 1101, 1174, 1444, 1504
MboI GATC 7 cut(s) 72, 129, 225, 1099, 1172, 1442, 1502
MboII GAAGA 3 cut(s) 690, 826, 1021
MfeI CAATTG 1 cut(s) 879
MflI RGATCY 2 cut(s) 72, 1172
MhlI GDGCHC 5 cut(s) 235, 961, 1188, 1261, 1539
MluCI AATT 9 cut(s) 113, 136, 217, 465, 494, 523, 671, 879, 998
MmeI TCCRAC 1 cut(s) 715
MseI TTAA 3 cut(s) 915, 975, 1452
MslI CAYNNNNRTG 2 cut(s) 533, 1254
MunI CAATTG 1 cut(s) 879
Mva1269I GAATGC 4 cut(s) 54, 810, 823, 1464
MvnI CGCG 1 cut(s) 1371
MwoI GCNNNNNNNGC 2 cut(s) 632, 1175
NdeII GATC 7 cut(s) 72, 129, 225, 1099, 1172, 1442, 1502
NheI GCTAGC 1 cut(s) 347
NlaIV GGNNCC 3 cut(s) 15, 1072, 1538
NmuCI GTSAC 1 cut(s) 709
NspI RCATGY 2 cut(s) 1253, 1265
NspV TTCGAA 2 cut(s) 187, 659
PaqCI CACCTGC 1 cut(s) 472
PauI GCGCGC 1 cut(s) 1369
PcsI WCGNNNNNNNCGW 1 cut(s) 1238
PctI GAATGC 4 cut(s) 54, 810, 823, 1464
PfeI GAWTC 4 cut(s) 184, 353, 399, 685
PkrI GCNGC 4 cut(s) 742, 1084, 1568, 1571
Ppu21I YACGTR 1 cut(s) 1544
PpuMI RGGWCCY 1 cut(s) 13
PsiI TTATAA 2 cut(s) 276, 1317
Psp124BI GAGCTC 1 cut(s) 1188
Psp1406I AACGTT 1 cut(s) 1232
Psp5II RGGWCCY 1 cut(s) 13
PspN4I GGNNCC 3 cut(s) 15, 1072, 1538
PspPI GGNCC 2 cut(s) 13, 97
PspPPI RGGWCCY 1 cut(s) 13
PstI CTGCAG 1 cut(s) 1571
PsuI RGATCY 2 cut(s) 72, 1172
PteI GCGCGC 1 cut(s) 1369
RsaI GTAC 3 cut(s) 430, 1039, 1563
RsaNI GTAC 3 cut(s) 429, 1038, 1562
RseI CAYNNNNRTG 2 cut(s) 533, 1254
SacI GAGCTC 1 cut(s) 1188
SaqAI TTAA 3 cut(s) 915, 975, 1452
SatI GCNGC 4 cut(s) 741, 1083, 1567, 1570
Sau3AI GATC 7 cut(s) 72, 129, 225, 1099, 1172, 1442, 1502
Sau96I GGNCC 2 cut(s) 13, 97
SduI GDGCHC 5 cut(s) 235, 961, 1188, 1261, 1539
SfcI CTRYAG 1 cut(s) 1567
SfuI TTCGAA 2 cut(s) 187, 659
SinI GGWCC 1 cut(s) 13
SmiMI CAYNNNNRTG 2 cut(s) 533, 1254
SmlI CTYRAG 1 cut(s) 192
SmoI CTYRAG 1 cut(s) 192
Sse9I AATT 9 cut(s) 113, 136, 217, 465, 494, 523, 671, 879, 998
SsiI CCGC 3 cut(s) 175, 626, 951
SspMI CTAG 2 cut(s) 348, 1086
SstI GAGCTC 1 cut(s) 1188
TaaI ACNGT 5 cut(s) 502, 655, 1120, 1346, 1449
TaiI ACGT 4 cut(s) 929, 1095, 1235, 1546
TaqI TCGA 9 cut(s) 39, 187, 269, 414, 547, 659, 869, 1241, 1501
TasI AATT 9 cut(s) 113, 136, 217, 465, 494, 523, 671, 879, 998
TatI WGTACW 1 cut(s) 428
TfiI GAWTC 4 cut(s) 184, 353, 399, 685
Tru1I TTAA 3 cut(s) 915, 975, 1452
Tru9I TTAA 3 cut(s) 915, 975, 1452
TscAI CASTG 1 cut(s) 1452
TseFI GTSAC 1 cut(s) 709
TseI GCWGC 4 cut(s) 740, 1082, 1566, 1569
Tsp45I GTSAC 1 cut(s) 709
TspDTI ATGAA 7 cut(s) 17, 106, 371, 507, 633, 677, 1457
TspRI CASTG 1 cut(s) 1452
VneI GTGCAC 1 cut(s) 1257
VpaK11BI GGWCC 1 cut(s) 13
XapI RAATTY 4 cut(s) 494, 523, 671, 998
XceI RCATGY 2 cut(s) 1253, 1265
XmiI GTMKAC 2 cut(s) 213, 1050
XspI CTAG 2 cut(s) 348, 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.