pycom15g14440

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0024689
Physical Location & Seq
Reverse (-)
9853721 .. 9854536
816 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
ATGCACTCTGTTGCTGTCCTGCAGGTGAAAGTCCTTTTCCTCATCCTTATGGATCTTTGCCGGCGAACAGCAAGCCTGGCACATTCATTGCCAGTTTCTCAGGTGGGAAGCAGCAACATTCAGGTTCGGCTGCATTATATCGATGGCAATTATACTGTACTTCCGGAGGTTGTTGAAGCATCCCTTGGCCCTCATATGCAGAATATGCCCCGGCCTAGAGGTGCAGATGCTGCTGGTCTATTACTCCGAGAGTTAGAACTCCACCCTCCAGCCGAAGAGTGGCATAGGCGCAATATGTATGGTGGGCCTTGGTCTGATTCAGATGATATGGATCCTCAAGATGATACCCCCAAGCTATGTAATTCTTCAGACCCACCTGATTCCGGATCTTTGGAAAGTTGTGATGTTTATTATGGAGCCCATGGCCTGTGGCCAAGGAAGCGCAGGATGTCTGAAAGAGATGCAGCTTTTGGCCTAAACACTTCAGTGGGTCTGGGGGCATATCTTGGTATAATGGGATCACGGAGAGATGTTGTTACTGCCGTATGGAAGACTGGTCTAGAAGGGGTTTGGTACAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

21.5

Weight (kDa)

5.95

Isoelectric Point (pI)

52.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024689)

Species Orthologous Gene IDs
pyrus_communis pycom13g18180 pycom15g14440
rosa_multiflora Rmu_co8144380.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 13
AccIII TCCGGA 2 cut(s) 163, 383
AclWI GGATC 5 cut(s) 60, 326, 339, 394, 526
AcoI YGGCCR 1 cut(s) 431
AcuI CTGAAG 2 cut(s) 351, 468
AfaI GTAC 2 cut(s) 159, 575
AfiI CCNNNNNNNGG 2 cut(s) 279, 383
AgsI TTSAA 1 cut(s) 176
AjnI CCWGG 1 cut(s) 75
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AleI CACNNNNGTG 1 cut(s) 485
AluBI AGCT 2 cut(s) 355, 467
AluI AGCT 2 cut(s) 355, 467
AlwI GGATC 5 cut(s) 60, 326, 339, 394, 526
AlwNI CAGNNNCTG 1 cut(s) 230
Aor13HI TCCGGA 2 cut(s) 163, 383
AoxI GGCC 6 cut(s) 187, 212, 305, 424, 431, 472
ApeKI GCWGC 4 cut(s) 111, 130, 230, 464
AspLEI GCGC 2 cut(s) 291, 444
AspS9I GGNCC 2 cut(s) 188, 305
AsuC2I CCSGG 1 cut(s) 211
AsuHPI GGTGA 1 cut(s) 37
BalI TGGCCA 1 cut(s) 433
BamHI GGATCC 1 cut(s) 331
BanII GRGCYC 1 cut(s) 421
BbsI GAAGAC 1 cut(s) 557
BbvI GCAGC 4 cut(s) 117, 123, 217, 476
BccI CCATC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 527
BciT130I CCWGG 1 cut(s) 77
BcnI CCSGG 1 cut(s) 211
BfaI CTAG 2 cut(s) 216, 560
BfmI CTRYAG 1 cut(s) 20
BfuAI ACCTGC 1 cut(s) 13
BisI GCNGC 4 cut(s) 112, 131, 231, 465
BlsI GCNGC 4 cut(s) 113, 132, 232, 466
Bme1390I CCNGG 2 cut(s) 77, 211
BmgT120I GGNCC 2 cut(s) 188, 305
BmiI GGNNCC 2 cut(s) 333, 418
BmrFI CCNGG 2 cut(s) 77, 211
BmsI GCATC 3 cut(s) 188, 217, 451
BpiI GAAGAC 1 cut(s) 557
BpmI CTGGAG 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 321
BpuMI CCSGG 1 cut(s) 211
Bsa29I ATCGAT 1 cut(s) 141
BsaBI GATNNNNATC 1 cut(s) 330
BsaJI CCNNGG 5 cut(s) 184, 209, 308, 421, 434
BsaWI WCCGGW 2 cut(s) 163, 383
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 383
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 2 cut(s) 92, 559
Bse3DI GCAATG 1 cut(s) 86
Bse8I GATNNNNATC 1 cut(s) 330
BseAI TCCGGA 2 cut(s) 163, 383
BseBI CCWGG 1 cut(s) 77
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 5 cut(s) 184, 209, 308, 421, 434
BseGI GGATG 3 cut(s) 42, 179, 453
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 2 cut(s) 279, 383
BseMI GCAATG 1 cut(s) 86
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 2 cut(s) 92, 559
BseXI GCAGC 4 cut(s) 117, 123, 217, 476
BsgI GTGCAG 1 cut(s) 243
BshFI GGCC 6 cut(s) 189, 214, 307, 426, 433, 474
BshVI ATCGAT 1 cut(s) 141
BsiSI CCGG 4 cut(s) 61, 164, 211, 384
BslI CCNNNNNNNGG 2 cut(s) 279, 383
BsnI GGCC 6 cut(s) 189, 214, 307, 426, 433, 474
Bsp1286I GDGCHC 1 cut(s) 421
Bsp13I TCCGGA 2 cut(s) 163, 383
Bsp143I GATC 4 cut(s) 52, 331, 386, 518
Bsp19I CCATGG 1 cut(s) 421
BspANI GGCC 6 cut(s) 189, 214, 307, 426, 433, 474
BspCNI CTCAG 1 cut(s) 112
BspDI ATCGAT 1 cut(s) 141
BspEI TCCGGA 2 cut(s) 163, 383
BspLI GGNNCC 2 cut(s) 333, 418
BspMAI CTGCAG 1 cut(s) 24
BspMI ACCTGC 1 cut(s) 13
BspPI GGATC 5 cut(s) 60, 326, 339, 394, 526
BsrDI GCAATG 1 cut(s) 86
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 2 cut(s) 92, 559
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 5 cut(s) 184, 209, 308, 421, 434
BssMI GATC 4 cut(s) 52, 331, 386, 518
BssT1I CCWWGG 4 cut(s) 184, 308, 421, 434
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 1 cut(s) 157
Bst6I CTCTTC 1 cut(s) 270
BstAPI GCANNNNNTGC 2 cut(s) 205, 230
BstC8I GCNNGC 2 cut(s) 62, 73
BstDEI CTNAG 1 cut(s) 99
BstDSI CCRYGG 1 cut(s) 421
BstF5I GGATG 3 cut(s) 42, 179, 453
BstHHI GCGC 2 cut(s) 291, 444
BstKTI GATC 4 cut(s) 55, 334, 389, 521
BstMBI GATC 4 cut(s) 52, 331, 386, 518
BstMWI GCNNNNNNNGC 4 cut(s) 77, 205, 230, 439
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 2 cut(s) 75, 209
BstSFI CTRYAG 1 cut(s) 20
BstV1I GCAGC 4 cut(s) 117, 123, 217, 476
BstV2I GAAGAC 1 cut(s) 557
BstX2I RGATCY 3 cut(s) 52, 331, 386
BstYI RGATCY 3 cut(s) 52, 331, 386
Bsu15I ATCGAT 1 cut(s) 141
BsuRI GGCC 6 cut(s) 189, 214, 307, 426, 433, 474
BsuTUI ATCGAT 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 421
BtsCI GGATG 3 cut(s) 42, 179, 453
BtsIMutI CAGTG 1 cut(s) 492
BveI ACCTGC 1 cut(s) 13
Cac8I GCNNGC 2 cut(s) 62, 73
CaiI CAGNNNCTG 1 cut(s) 230
CfoI GCGC 2 cut(s) 291, 444
Cfr10I RCCGGY 1 cut(s) 60
Cfr13I GGNCC 2 cut(s) 188, 305
ClaI ATCGAT 1 cut(s) 141
Csp6I GTAC 2 cut(s) 158, 574
CviAII CATG 1 cut(s) 422
CviQI GTAC 2 cut(s) 158, 574
DdeI CTNAG 1 cut(s) 99
DpnI GATC 4 cut(s) 54, 333, 388, 520
DpnII GATC 4 cut(s) 52, 331, 386, 518
EaeI YGGCCR 1 cut(s) 431
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Eco130I CCWWGG 4 cut(s) 184, 308, 421, 434
Eco24I GRGCYC 1 cut(s) 421
Eco57I CTGAAG 2 cut(s) 351, 468
EcoRII CCWGG 1 cut(s) 75
EcoT14I CCWWGG 4 cut(s) 184, 308, 421, 434
EcoT38I GRGCYC 1 cut(s) 421
ErhI CCWWGG 4 cut(s) 184, 308, 421, 434
FaeI CATG 1 cut(s) 425
FalI AAGNNNNNCTT 2 cut(s) 168, 200
FatI CATG 1 cut(s) 421
FauNDI CATATG 1 cut(s) 195
Fnu4HI GCNGC 4 cut(s) 112, 131, 231, 465
FokI GGATG 3 cut(s) 29, 166, 460
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 4 cut(s) 112, 131, 231, 465
FspBI CTAG 2 cut(s) 216, 560
GlaI GCGC 2 cut(s) 290, 443
GluI GCNGC 4 cut(s) 112, 131, 231, 465
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 6 cut(s) 189, 214, 307, 426, 433, 474
HapII CCGG 4 cut(s) 61, 164, 211, 384
HhaI GCGC 2 cut(s) 291, 444
Hin1II CATG 1 cut(s) 425
Hin6I GCGC 2 cut(s) 289, 442
HinP1I GCGC 2 cut(s) 289, 442
HinfI GANTC 2 cut(s) 317, 380
HpaII CCGG 4 cut(s) 61, 164, 211, 384
HphI GGTGA 1 cut(s) 37
Hpy188I TCNGA 5 cut(s) 248, 316, 322, 370, 454
Hpy188III TCNNGA 4 cut(s) 164, 338, 384, 560
HpyAV CCTTC 1 cut(s) 557
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4V TGCA 6 cut(s) 4, 22, 133, 199, 224, 464
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 205, 230, 439
HpyF3I CTNAG 1 cut(s) 99
Hsp92II CATG 1 cut(s) 425
HspAI GCGC 2 cut(s) 289, 442
Kpn2I TCCGGA 2 cut(s) 163, 383
KroI GCCGGC 1 cut(s) 60
KroNI GCCGGC 1 cut(s) 62
Kzo9I GATC 4 cut(s) 52, 331, 386, 518
LmnI GCTCC 1 cut(s) 416
Lsp1109I GCAGC 4 cut(s) 117, 123, 217, 476
LweI GCATC 3 cut(s) 188, 217, 451
MaeI CTAG 2 cut(s) 216, 560
MaeIII GTNAC 1 cut(s) 535
MalI GATC 4 cut(s) 54, 333, 388, 520
MboI GATC 4 cut(s) 52, 331, 386, 518
MboII GAAGA 3 cut(s) 287, 357, 562
MflI RGATCY 3 cut(s) 52, 331, 386
MhlI GDGCHC 1 cut(s) 421
MlsI TGGCCA 1 cut(s) 433
MluCI AATT 2 cut(s) 148, 361
MluNI TGGCCA 1 cut(s) 433
MnlI CCTC 6 cut(s) 50, 160, 201, 212, 276, 345
Mox20I TGGCCA 1 cut(s) 433
MroI TCCGGA 2 cut(s) 163, 383
MroNI GCCGGC 1 cut(s) 60
MscI TGGCCA 1 cut(s) 433
MslI CAYNNNNRTG 2 cut(s) 47, 485
Msp20I TGGCCA 1 cut(s) 433
MspI CCGG 4 cut(s) 61, 164, 211, 384
MspR9I CCNGG 2 cut(s) 77, 211
MvaI CCWGG 1 cut(s) 77
MwoI GCNNNNNNNGC 4 cut(s) 77, 205, 230, 439
NaeI GCCGGC 1 cut(s) 62
NciI CCSGG 1 cut(s) 211
NcoI CCATGG 1 cut(s) 421
NdeI CATATG 1 cut(s) 195
NdeII GATC 4 cut(s) 52, 331, 386, 518
NgoMIV GCCGGC 1 cut(s) 60
NlaIII CATG 1 cut(s) 425
NlaIV GGNNCC 2 cut(s) 333, 418
OliI CACNNNNGTG 1 cut(s) 485
PaqCI CACCTGC 1 cut(s) 13
PdiI GCCGGC 1 cut(s) 62
PfeI GAWTC 2 cut(s) 317, 380
PkrI GCNGC 4 cut(s) 113, 132, 232, 466
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 2 cut(s) 333, 418
PspPI GGNCC 2 cut(s) 188, 305
PstI CTGCAG 1 cut(s) 24
PstNI CAGNNNCTG 1 cut(s) 230
PsuI RGATCY 3 cut(s) 52, 331, 386
RsaI GTAC 2 cut(s) 159, 575
RsaNI GTAC 2 cut(s) 158, 574
RseI CAYNNNNRTG 2 cut(s) 47, 485
SatI GCNGC 4 cut(s) 112, 131, 231, 465
Sau3AI GATC 4 cut(s) 52, 331, 386, 518
Sau96I GGNCC 2 cut(s) 188, 305
SbfI CCTGCAGG 1 cut(s) 24
ScrFI CCNGG 2 cut(s) 77, 211
SdaI CCTGCAGG 1 cut(s) 24
SduI GDGCHC 1 cut(s) 421
SetI ASST 9 cut(s) 27, 105, 126, 171, 223, 357, 379, 469, 582
SfaNI GCATC 3 cut(s) 188, 217, 451
SfcI CTRYAG 1 cut(s) 20
SmiMI CAYNNNNRTG 2 cut(s) 47, 485
SmlI CTYRAG 1 cut(s) 336
SmoI CTYRAG 1 cut(s) 336
Sse8387I CCTGCAGG 1 cut(s) 24
Sse9I AATT 2 cut(s) 148, 361
SspMI CTAG 2 cut(s) 216, 560
StyD4I CCNGG 2 cut(s) 75, 209
StyI CCWWGG 4 cut(s) 184, 308, 421, 434
TaaI ACNGT 1 cut(s) 157
TaqI TCGA 1 cut(s) 141
TasI AATT 2 cut(s) 148, 361
TatI WGTACW 1 cut(s) 157
TfiI GAWTC 2 cut(s) 317, 380
TscAI CASTG 1 cut(s) 492
TseI GCWGC 4 cut(s) 111, 130, 230, 464
TspDTI ATGAA 1 cut(s) 75
TspGWI ACGGA 1 cut(s) 538
TspRI CASTG 1 cut(s) 492
XbaI TCTAGA 1 cut(s) 559
XcmI CCANNNNNNNNNTGG 1 cut(s) 276
XspI CTAG 2 cut(s) 216, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.