Rmu_co8144380.1_g000001

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8144380.1
OG0024689
Physical Location & Seq
Reverse (-)
1 .. 620
620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8144380.1_g000001.1.cds

Sequence Viewer

Length: 507 bp
atgccccggcctagaggtgctgatgctgctggtctgttactccgagaattagaactccaccctccagctgaagagtggcacagacggaatatgtatggtgggccttggtctgatccggatgatgtgggccttctagatgatactcccaagctatgtaattctcatgacccagtcgatttgggattgatggaaagttctgatgtctattatggagcccgtggcctgtggccgaggaagcgcagaatgtctgaaagagatgcagcttttggcttaaacacatctgtgggtctaggggcgtatcttggtataatgggatcacggagagatgttgttactgctgtatggaagactggtcttgatggtgtttggtataagtgcatcagatgtttacggcagacttcagcttttgcttcccaaggtgcttctagtccacctaatcaaactgagcgggagacttggtggatcagccgctgggcttattgctgtcctatgtgtggtggaacatgg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

18.96

Weight (kDa)

6.81

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024689)

Species Orthologous Gene IDs
pyrus_communis pycom13g18180 pycom15g14440
rosa_multiflora Rmu_co8144380.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 448
AccIII TCCGGA 1 cut(s) 115
AciI CCGC 2 cut(s) 448, 469
AclWI GGATC 3 cut(s) 107, 322, 470
AcoI YGGCCR 1 cut(s) 227
AcuI CTGAAG 2 cut(s) 90, 384
AfiI CCNNNNNNNGG 1 cut(s) 494
AleI CACNNNNGTG 1 cut(s) 281
AluBI AGCT 4 cut(s) 68, 151, 263, 404
AluI AGCT 4 cut(s) 68, 151, 263, 404
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 3 cut(s) 107, 322, 470
AlwNI CAGNNNCTG 1 cut(s) 471
Aor13HI TCCGGA 1 cut(s) 115
AoxI GGCC 5 cut(s) 8, 101, 127, 220, 227
ApeKI GCWGC 2 cut(s) 26, 260
AspLEI GCGC 1 cut(s) 240
AspS9I GGNCC 2 cut(s) 101, 127
AsuC2I CCSGG 1 cut(s) 7
BanII GRGCYC 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 353
BbvI GCAGC 2 cut(s) 13, 272
BccI CCATC 2 cut(s) 181, 353
BceAI ACGGC 1 cut(s) 407
BcnI CCSGG 1 cut(s) 7
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 4 cut(s) 12, 134, 290, 426
BisI GCNGC 3 cut(s) 27, 261, 469
BlsI GCNGC 3 cut(s) 28, 262, 470
Bme1390I CCNGG 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 101, 127
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 1 cut(s) 7
BmrI ACTGGG 1 cut(s) 164
BmsI GCATC 3 cut(s) 13, 247, 387
BmuI ACTGGG 1 cut(s) 164
BpiI GAAGAC 1 cut(s) 353
BpmI CTGGAG 1 cut(s) 48
BpuMI CCSGG 1 cut(s) 7
BsaJI CCNNGG 5 cut(s) 5, 104, 217, 230, 415
BsaWI WCCGGW 1 cut(s) 115
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 1 cut(s) 494
Bse1I ACTGG 2 cut(s) 170, 355
BseAI TCCGGA 1 cut(s) 115
BseDI CCNNGG 5 cut(s) 5, 104, 217, 230, 415
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 494
BseMII CTCAG 1 cut(s) 435
BseNI ACTGG 2 cut(s) 170, 355
BseXI GCAGC 2 cut(s) 13, 272
BseYI CCCAGC 1 cut(s) 471
BshFI GGCC 5 cut(s) 10, 103, 129, 222, 229
BsiSI CCGG 2 cut(s) 7, 116
BslI CCNNNNNNNGG 1 cut(s) 494
BsmAI GTCTC 1 cut(s) 446
BsnI GGCC 5 cut(s) 10, 103, 129, 222, 229
Bsp1286I GDGCHC 1 cut(s) 217
Bsp13I TCCGGA 1 cut(s) 115
Bsp143I GATC 3 cut(s) 112, 314, 462
BspACI CCGC 2 cut(s) 448, 469
BspANI GGCC 5 cut(s) 10, 103, 129, 222, 229
BspCNI CTCAG 1 cut(s) 436
BspEI TCCGGA 1 cut(s) 115
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 214
BspPI GGATC 3 cut(s) 107, 322, 470
BsrBI CCGCTC 1 cut(s) 448
BsrI ACTGG 2 cut(s) 170, 355
BssECI CCNNGG 5 cut(s) 5, 104, 217, 230, 415
BssMI GATC 3 cut(s) 112, 314, 462
BssT1I CCWWGG 2 cut(s) 104, 415
Bst6I CTCTTC 1 cut(s) 66
BstDEI CTNAG 1 cut(s) 444
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 1 cut(s) 124
BstHHI GCGC 1 cut(s) 240
BstKTI GATC 3 cut(s) 115, 317, 465
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 3 cut(s) 112, 314, 462
BstMWI GCNNNNNNNGC 2 cut(s) 26, 235
BstSCI CCNGG 1 cut(s) 5
BstV1I GCAGC 2 cut(s) 13, 272
BstV2I GAAGAC 1 cut(s) 353
BsuRI GGCC 5 cut(s) 10, 103, 129, 222, 229
BtgI CCRYGG 1 cut(s) 217
BtsCI GGATG 1 cut(s) 124
CaiI CAGNNNCTG 1 cut(s) 471
CciI TCATGA 1 cut(s) 163
CfoI GCGC 1 cut(s) 240
Cfr13I GGNCC 2 cut(s) 101, 127
CviAII CATG 2 cut(s) 164, 504
DdeI CTNAG 1 cut(s) 444
DpnI GATC 3 cut(s) 114, 316, 464
DpnII GATC 3 cut(s) 112, 314, 462
EaeI YGGCCR 1 cut(s) 227
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
Eco130I CCWWGG 2 cut(s) 104, 415
Eco24I GRGCYC 1 cut(s) 217
Eco57I CTGAAG 2 cut(s) 90, 384
EcoT14I CCWWGG 2 cut(s) 104, 415
EcoT38I GRGCYC 1 cut(s) 217
ErhI CCWWGG 2 cut(s) 104, 415
FaeI CATG 2 cut(s) 167, 507
FatI CATG 2 cut(s) 163, 503
FauI CCCGC 1 cut(s) 441
Fnu4HI GCNGC 3 cut(s) 27, 261, 469
FokI GGATG 1 cut(s) 131
FriOI GRGCYC 1 cut(s) 217
Fsp4HI GCNGC 3 cut(s) 27, 261, 469
FspBI CTAG 4 cut(s) 12, 134, 290, 426
GlaI GCGC 1 cut(s) 239
GluI GCNGC 3 cut(s) 27, 261, 469
GsaI CCCAGC 1 cut(s) 475
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 5 cut(s) 10, 103, 129, 222, 229
HapII CCGG 2 cut(s) 7, 116
HhaI GCGC 1 cut(s) 240
Hin1II CATG 2 cut(s) 167, 507
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HpaII CCGG 2 cut(s) 7, 116
Hpy166II GTNNAC 2 cut(s) 389, 431
Hpy188I TCNGA 5 cut(s) 44, 112, 199, 250, 383
Hpy188III TCNNGA 4 cut(s) 116, 134, 164, 356
Hpy8I GTNNAC 2 cut(s) 389, 431
HpyAV CCTTC 1 cut(s) 140
HpyCH4V TGCA 2 cut(s) 260, 378
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 235
HpyF3I CTNAG 1 cut(s) 444
Hsp92II CATG 2 cut(s) 167, 507
HspAI GCGC 1 cut(s) 238
Kpn2I TCCGGA 1 cut(s) 115
Kzo9I GATC 3 cut(s) 112, 314, 462
LmnI GCTCC 1 cut(s) 212
LpnPI CCDG 8 cut(s) 15, 20, 78, 129, 183, 236, 336, 457
Lsp1109I GCAGC 2 cut(s) 13, 272
LweI GCATC 3 cut(s) 13, 247, 387
MaeI CTAG 4 cut(s) 12, 134, 290, 426
MaeIII GTNAC 2 cut(s) 36, 331
MalI GATC 3 cut(s) 114, 316, 464
MbiI CCGCTC 1 cut(s) 448
MboI GATC 3 cut(s) 112, 314, 462
MboII GAAGA 2 cut(s) 83, 358
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 2 cut(s) 47, 157
MnlI CCTC 3 cut(s) 8, 72, 225
MroI TCCGGA 1 cut(s) 115
MseI TTAA 1 cut(s) 272
MslI CAYNNNNRTG 1 cut(s) 281
MspA1I CMGCKG 2 cut(s) 68, 471
MspI CCGG 2 cut(s) 7, 116
MspR9I CCNGG 1 cut(s) 7
MwoI GCNNNNNNNGC 2 cut(s) 26, 235
NciI CCSGG 1 cut(s) 7
NdeII GATC 3 cut(s) 112, 314, 462
NlaIII CATG 2 cut(s) 167, 507
NlaIV GGNNCC 1 cut(s) 214
NmeAIII GCCGAG 1 cut(s) 255
OliI CACNNNNGTG 1 cut(s) 281
PagI TCATGA 1 cut(s) 163
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 3 cut(s) 28, 262, 470
PspFI CCCAGC 1 cut(s) 471
PspN4I GGNNCC 1 cut(s) 214
PspPI GGNCC 2 cut(s) 101, 127
PstNI CAGNNNCTG 1 cut(s) 471
PsyI GACNNNGTC 1 cut(s) 170
PvuII CAGCTG 1 cut(s) 68
RseI CAYNNNNRTG 1 cut(s) 281
SaqAI TTAA 1 cut(s) 272
SatI GCNGC 3 cut(s) 27, 261, 469
Sau3AI GATC 3 cut(s) 112, 314, 462
Sau96I GGNCC 2 cut(s) 101, 127
ScrFI CCNGG 1 cut(s) 7
SduI GDGCHC 1 cut(s) 217
SetI ASST 7 cut(s) 19, 70, 153, 265, 406, 421, 436
SfaNI GCATC 3 cut(s) 13, 247, 387
SmiMI CAYNNNNRTG 1 cut(s) 281
Sse9I AATT 2 cut(s) 47, 157
SsiI CCGC 2 cut(s) 448, 469
SspMI CTAG 4 cut(s) 12, 134, 290, 426
StyD4I CCNGG 1 cut(s) 5
StyI CCWWGG 2 cut(s) 104, 415
TaqI TCGA 1 cut(s) 174
TasI AATT 2 cut(s) 47, 157
TauI GCSGC 1 cut(s) 471
Tru1I TTAA 1 cut(s) 272
Tru9I TTAA 1 cut(s) 272
TseI GCWGC 2 cut(s) 26, 260
TspGWI ACGGA 2 cut(s) 100, 334
Tth111I GACNNNGTC 1 cut(s) 170
XbaI TCTAGA 1 cut(s) 133
XcmI CCANNNNNNNNNTGG 1 cut(s) 72
XspI CTAG 4 cut(s) 12, 134, 290, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.