pycom15g32450

actin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0028847
Physical Location & Seq
Forward (+)
31453047 .. 31457535
4489 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g32450.1

Sequence Viewer

Length: 2892 bp
ATGGATACCAATACAGCCCTTGAAGCCTCCTGTCCATCCTCACCTTCCTCCCCCACATCCTCCTCCTCCTCAATAGAAGATGAGATTTACGATGTGTTCTTGAGCTTTAGTGGTGAAGACACACGCAAAACATTCACAGACCACCTCTACTGGACATTGAAAGCTGCCAGAGTTGACACCTTTATTGACGAGAATGAGTCAATGAGCATAGGGGAAACTATATCAGACAAAGTGAAGCAAGCTATCCAAGGGTCTAGGATCGCTGTCATCATCTTCTCAAGGAGGTATGCGGATTCCATCCGGTGTCTTGAGGAGCTGGTGAAGATCATGGAGTGTAAAAGAACAATGGGGCAAATGGTTTTGCCAATATTCTATGATGTTGATCCTTCGGATGTCAAGAATCAGTCTGGGACTTTCGCAGAAGCATTTCAGAAACACAAAGACCGCTTCCATCAAGTTAAAGATAAGAAAGAAAAGCTAGGGCCGTGGAGAAACGCTCTTACTGAAGCTGCAGATTTGCCCAGTGAGAATTTTGCAAAGACTGACGGGTATCAAGGAGTGTTTATCACGAAAATTGTTGACGAGATCACTAGAAAACTGAAGAGCACATCCTTAGGCGTAGCCACGGGACAAGATGGAATACATTCTCAAGTGCAAGCTGGAATAGATTCCCCCCTACAAGAAATCAGTTACTATACAGATGTTCAAGTTGGATTAGATTCTCGTGTGCAAGAAATTTGTAATTATTTAGATGGTGTTGGAGGATCAAATGATGTTCGCATGATTCGAATCTGTGGTATGGGTGGAATAGGCATGCATGATATGATTCAAGACATGGGCAGAGAAATCATGCTTGCAGAATCCTCGGGCAACCCTGAAGAACGTAGCAGATTGCGGGTTCCTAAAGATGTAGAAGGTGTATTGAGGAATAACTCTGGAACGAGAAAGCTAATGGGACCAAGTTTAGATTTGCATAAACCTGACAAGCCTGGCTGCAGTACAGAATCATTTCAAGATATGCAAATTCTGAGTGCGCTCAAACTCACTGGAAGCTGCATGCATCATTCTACTTGCCTGGCACGATCCCCAGACTTTCTAGAAATCATAGAACTAGAGAAACTGAGACTCAAAGACTTGCCGGGTTTGTCTGTGATTCACCGTTCCGCAGCACACCTGTTTAACCTTAATTATTTATCACCAGACTTCCTGATGGAACTGATAATCGATGACTGCAAGAGTTTGTCCAAGTTTCTCCCTTCCATGATACAACTGAAGAACCTTAAGTATTTATATCTTGAGAACTGCAACTTAACAAATGATGCAATTCCAAAGGATCTTGGGGGTCTATCTTCTTTAGAAGATTTAGATCTAAGTGGCAATGAATTTAGTGGGCTACCTTCCCTCAGTGGCCTTTCCAAACTTCGAGCATTGCAATTGCGTAATTGTAAAAGCCTTCAGTCAATTCCAGATTTACCAAAGCAATTGATAATCTTGGTAGCAAATGGGTGCATTGCTCTGAAAAAAATGCCAGATTTTTCAGATATGTCGAGTATAAGAGAATTGTATCTGAATCACTCCCCCAAACTCACTGAGCCAATAATCCTGCAGCGGCTTGAAAAGTTGGAGCTTCTCGATCTTGGGTATTGTCATCGCCTGAAAACATCACCGGACTTTTCAAAACTCCCAAATCTCAACATTCTGTTTCTCAATGACTGCAAGAGTTTGTCTGAGATTCACCCTTCCCTCAGTGGCCTTTCCAAGCTTCAGTCTTTGTATCTTGAGAACTGCAACTTAACAAATGATGCAATTCCAAAGGATCGATCCCTACTTGGGGGTCTATCTTCTTTAGAAGTTTTAGATCTCAGTGGCAATGAATTTAGTGGGCTACCTTCCCTCAGTGGCCTTTCCGAGCTTCGGTCTTTATATCTTAATAACTGCAACTTAACAAATGATGCAATTCCAAAGGACCTTGGGGGTCTATCTTCTTTAGAAGTTTTGGATCTCAGTGGCAATGAATTTAGTGGGCTACCTTCCCTCAGTGGCCTTTCCGAGCTTTGGTGTTTATATCTTAAGAACTGCAACTTAACAAATGATGCAATTCCGAAGGATCTTAGGGGTCTATCTTCTTTAAAAGTTTTAGATCTCAGTGGCAATGAATTTAGTGGGCTGCCTTCCCTCAGTGGCCTTTCCAAGCTTCGGTGTTTATATCTTAAGAACTGCAACTTAACAAATGATGCAATTCCGAAGGATCTTAGGGGTCTATCTTCTTTAAAAGTTTTAGATCTCAGTGGCAATGAATTTAGTGGGCTGCCTTCCCTCAGTGGCCTTTCCAAGCTTCGGTGTTTATATCTTAAGAACTGCAACTTAACAAATTATGCAATTCCAAAGGATCTTGGGGGTCTATCTTCGTTAAAAGTTTTAGATCTCAGTGGCAATGAATTTAGTGGGCTACCTTCCCTCAGTGGCCTTTCCAAGCTTCGGTGTTTATATCTTAAGAACTGCAACTTAACAAATGATGCAACTCCAAAGGATCTTGGGGGTCTATCTTCTTTAGAAGAAGTGGCAATGATTTTAGTGGGCTACCCTGTGGGAAAGAGGATTTATTCTTCTTCTGCAGCTTCCTCTTCTGATGAACAAGAAGAAGAAGAAGAAGAAGGAGAGGTTGGAGAGGAGGGTGCTATGGAGATTGAGATTAGAGATACTGTGCCGGTCTCTGAAGGGTGTTCATTTACTACGTCGAATGATGATGATTCTTCCAGCACCACCACTGTTTCTCCGAATGAGAGAATTTCGCAGAATGGGAGGCTGAATTGTATTATCACTCACTGGGAGAAGGGTTACCTTCTTGGCAGTGGGTCTTTCGGGTCAGTGTATGAAGCAATTTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 map04011
Pfam Domains
Protein Families
Drf_FH3 Drf_GBD FH2

Protein Analysis

964

Amino Acids

105.54

Weight (kDa)

5.02

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 30 - 204 1.1e-46 TIR domain
TIR_2 PF13676 32 - 130 8.4e-15 TIR domain
LRR_14 PF23598 415 - 495 8.9e-06 Leucine-rich repeat region
LRR_8 PF13855 426 - 482 6.7e-06 Leucine rich repeat
LRR_Zer-1 PF25013 534 - 628 2.7e-06 Zer-1-like, Leucine-rich repeats
LRR_8 PF13855 608 - 648 6.3e-06 Leucine rich repeat
LRR_4 PF12799 615 - 649 9.2e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 615 - 715 1.1e-10 Leucine-rich repeat region
LRR_14 PF23598 665 - 762 8.2e-09 Leucine-rich repeat region
LRR_8 PF13855 705 - 742 7.2e-06 Leucine rich repeat
LRR_14 PF23598 706 - 787 1.1e-08 Leucine-rich repeat region
LRR_8 PF13855 752 - 789 7.2e-06 Leucine rich repeat
LRR_14 PF23598 754 - 858 3.1e-12 Leucine-rich repeat region
LRR_8 PF13855 799 - 836 8.8e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028847)

Species Orthologous Gene IDs
malus_domestica MD07G1102600.v1.1
pyrus_communis pycom15g32450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 290, 445, 895, 1164, 1609
AcuI CTGAAG 7 cut(s) 525, 620, 897, 1292, 1439, 1748, 2736
AfaI GTAC 1 cut(s) 1000
AfiI CCNNNNNNNGG 7 cut(s) 1830, 1831, 1914, 2055, 2196, 2337, 2478
AflII CTTAAG 5 cut(s) 1280, 2069, 2210, 2351, 2492
AgsI TTSAA 7 cut(s) 23, 160, 707, 830, 1013, 1616, 1677
AjnI CCWGG 2 cut(s) 988, 1074
AjuI GAANNNNNNNTTGG 2 cut(s) 2646, 2678
Alw21I GWGCWC 1 cut(s) 608
Alw26I GTCTC 2 cut(s) 1117, 2716
Ama87I CYCGRG 1 cut(s) 865
AoxI GGCC 8 cut(s) 482, 1408, 1750, 1900, 2041, 2182, 2323, 2464
ApeKI GCWGC 9 cut(s) 164, 509, 993, 1053, 1166, 1606, 2167, 2308, 2615
Asp700I GAANNNNTTC 4 cut(s) 426, 643, 667, 1008
AspLEI GCGC 1 cut(s) 1036
AspS9I GGNCC 3 cut(s) 482, 956, 1966
AsuC2I CCSGG 1 cut(s) 1140
AsuHPI GGTGA 7 cut(s) 33, 125, 331, 1148, 1188, 1656, 1727
AsuII TTCGAA 1 cut(s) 787
AvaI CYCGRG 1 cut(s) 865
AvaII GGWCC 2 cut(s) 956, 1966
AxyI CCTNAGG 1 cut(s) 613
BauI CACGAG 1 cut(s) 723
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 608
BbvI GCAGC 9 cut(s) 151, 496, 980, 1040, 1178, 1618, 2154, 2295, 2627
BccI CCATC 6 cut(s) 43, 305, 459, 629, 746, 1204
BceAI ACGGC 1 cut(s) 469
BcgI CGANNNNNNTGC 2 cut(s) 846, 880
BciT130I CCWGG 2 cut(s) 990, 1076
BcnI CCSGG 1 cut(s) 1140
BcoDI GTCTC 2 cut(s) 1117, 2716
BfaI CTAG 5 cut(s) 255, 479, 591, 1097, 1112
BfmI CTRYAG 4 cut(s) 510, 994, 1604, 2613
BfrI CTTAAG 5 cut(s) 1280, 2069, 2210, 2351, 2492
BglII AGATCT 5 cut(s) 1366, 1858, 2140, 2281, 2422
Bme1390I CCNGG 3 cut(s) 990, 1076, 1140
Bme18I GGWCC 2 cut(s) 956, 1966
BmeT110I CYCGRG 1 cut(s) 865
BmgT120I GGNCC 3 cut(s) 482, 956, 1966
BmiI GGNNCC 2 cut(s) 900, 957
BmrFI CCNGG 3 cut(s) 990, 1076, 1140
BmrI ACTGGG 2 cut(s) 516, 2836
BmsI GCATC 7 cut(s) 1069, 1309, 1792, 1942, 2083, 2224, 2506
BmuI ACTGGG 2 cut(s) 516, 2836
BpiI GAAGAC 1 cut(s) 123
BplI GAGNNNNNCTC 2 cut(s) 481, 513
Bpu14I TTCGAA 1 cut(s) 787
BpuEI CTTGAG 6 cut(s) 121, 262, 329, 633, 1316, 1799
BpuMI CCSGG 1 cut(s) 1140
Bsa29I ATCGAT 2 cut(s) 1224, 1819
BsaBI GATNNNNATC 3 cut(s) 381, 788, 1365
BsaI GGTCTC 1 cut(s) 2716
BsaJI CCNNGG 5 cut(s) 247, 485, 624, 864, 1969
BsaWI WCCGGW 2 cut(s) 300, 1666
BsaXI ACNNNNNCTCC 2 cut(s) 2758, 2788
Bsc4I CCNNNNNNNGG 7 cut(s) 1830, 1831, 1914, 2055, 2196, 2337, 2478
Bse118I RCCGGY 1 cut(s) 2707
Bse1I ACTGG 4 cut(s) 155, 522, 1051, 2831
Bse21I CCTNAGG 1 cut(s) 613
Bse3DI GCAATG 9 cut(s) 1384, 1427, 1509, 1876, 2017, 2158, 2299, 2440, 2571
Bse8I GATNNNNATC 3 cut(s) 381, 788, 1365
BseBI CCWGG 2 cut(s) 990, 1076
BseCI ATCGAT 2 cut(s) 1224, 1819
BseDI CCNNGG 5 cut(s) 247, 485, 624, 864, 1969
BseGI GGATG 5 cut(s) 35, 56, 297, 397, 608
BseJI GATNNNNATC 3 cut(s) 381, 788, 1365
BseLI CCNNNNNNNGG 7 cut(s) 1830, 1831, 1914, 2055, 2196, 2337, 2478
BseMI GCAATG 9 cut(s) 1384, 1427, 1509, 1876, 2017, 2158, 2299, 2440, 2571
BseNI ACTGG 4 cut(s) 155, 522, 1051, 2831
BseRI GAGGAG 5 cut(s) 52, 55, 58, 326, 2684
BseXI GCAGC 9 cut(s) 151, 496, 980, 1040, 1178, 1618, 2154, 2295, 2627
BshFI GGCC 8 cut(s) 484, 1410, 1752, 1902, 2043, 2184, 2325, 2466
BshVI ATCGAT 2 cut(s) 1224, 1819
BsiHKAI GWGCWC 1 cut(s) 608
BsiHKCI CYCGRG 1 cut(s) 865
BsiSI CCGG 4 cut(s) 301, 1139, 1667, 2708
BslFI GGGAC 3 cut(s) 424, 642, 969
BslI CCNNNNNNNGG 7 cut(s) 1830, 1831, 1914, 2055, 2196, 2337, 2478
BsmAI GTCTC 2 cut(s) 1117, 2716
BsmFI GGGAC 3 cut(s) 424, 642, 969
BsnI GGCC 8 cut(s) 484, 1410, 1752, 1902, 2043, 2184, 2325, 2466
Bso31I GGTCTC 1 cut(s) 2716
BsoBI CYCGRG 1 cut(s) 865
Bsp119I TTCGAA 1 cut(s) 787
Bsp1286I GDGCHC 1 cut(s) 608
BspACI CCGC 5 cut(s) 290, 445, 895, 1164, 1609
BspANI GGCC 8 cut(s) 484, 1410, 1752, 1902, 2043, 2184, 2325, 2466
BspDI ATCGAT 2 cut(s) 1224, 1819
BspLI GGNNCC 2 cut(s) 900, 957
BspMAI CTGCAG 4 cut(s) 514, 998, 1608, 2617
BspQI GCTCTTC 1 cut(s) 596
BspT104I TTCGAA 1 cut(s) 787
BspTI CTTAAG 5 cut(s) 1280, 2069, 2210, 2351, 2492
BspTNI GGTCTC 1 cut(s) 2716
BsrDI GCAATG 9 cut(s) 1384, 1427, 1509, 1876, 2017, 2158, 2299, 2440, 2571
BsrFI RCCGGY 1 cut(s) 2707
BsrI ACTGG 4 cut(s) 155, 522, 1051, 2831
BssAI RCCGGY 1 cut(s) 2707
BssECI CCNNGG 5 cut(s) 247, 485, 624, 864, 1969
BssSI CACGAG 1 cut(s) 723
BssT1I CCWWGG 2 cut(s) 247, 1969
Bst2BI CACGAG 1 cut(s) 723
Bst2UI CCWGG 2 cut(s) 990, 1076
Bst4CI ACNGT 3 cut(s) 1160, 2704, 2770
Bst6I CTCTTC 2 cut(s) 596, 2629
BstAFI CTTAAG 5 cut(s) 1280, 2069, 2210, 2351, 2492
BstBI TTCGAA 1 cut(s) 787
BstC8I GCNNGC 5 cut(s) 240, 657, 815, 855, 1058
BstDSI CCRYGG 2 cut(s) 485, 624
BstEII GGTNACC 1 cut(s) 2837
BstF5I GGATG 5 cut(s) 35, 56, 297, 397, 608
BstHHI GCGC 1 cut(s) 1036
BstMAI GTCTC 2 cut(s) 1117, 2716
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 2 cut(s) 990, 1076
BstNSI RCATGY 2 cut(s) 817, 1060
BstPI GGTNACC 1 cut(s) 2837
BstSCI CCNGG 3 cut(s) 988, 1074, 1138
BstSFI CTRYAG 4 cut(s) 510, 994, 1604, 2613
BstV1I GCAGC 9 cut(s) 151, 496, 980, 1040, 1178, 1618, 2154, 2295, 2627
BstV2I GAAGAC 1 cut(s) 123
Bsu15I ATCGAT 2 cut(s) 1224, 1819
Bsu36I CCTNAGG 1 cut(s) 613
BsuRI GGCC 8 cut(s) 484, 1410, 1752, 1902, 2043, 2184, 2325, 2466
BsuTUI ATCGAT 2 cut(s) 1224, 1819
BtgI CCRYGG 2 cut(s) 485, 624
BtgZI GCGATG 1 cut(s) 1634
BtsCI GGATG 5 cut(s) 35, 56, 297, 397, 608
BtsI GCAGTG 1 cut(s) 2857
Cac8I GCNNGC 5 cut(s) 240, 657, 815, 855, 1058
CfoI GCGC 1 cut(s) 1036
Cfr10I RCCGGY 1 cut(s) 2707
Cfr13I GGNCC 3 cut(s) 482, 956, 1966
ClaI ATCGAT 2 cut(s) 1224, 1819
Csp6I GTAC 1 cut(s) 999
CviAII CATG 8 cut(s) 328, 781, 814, 818, 835, 850, 1057, 1261
CviQI GTAC 1 cut(s) 999
DraI TTTAAA 2 cut(s) 2130, 2271
Eam1104I CTCTTC 2 cut(s) 596, 2629
EarI CTCTTC 2 cut(s) 596, 2629
Eco130I CCWWGG 2 cut(s) 247, 1969
Eco31I GGTCTC 1 cut(s) 2716
Eco47I GGWCC 2 cut(s) 956, 1966
Eco57I CTGAAG 7 cut(s) 525, 620, 897, 1292, 1439, 1748, 2736
Eco81I CCTNAGG 1 cut(s) 613
Eco88I CYCGRG 1 cut(s) 865
Eco91I GGTNACC 1 cut(s) 2837
EcoO109I RGGNCCY 1 cut(s) 1966
EcoO65I GGTNACC 1 cut(s) 2837
EcoRII CCWGG 2 cut(s) 988, 1074
EcoT14I CCWWGG 2 cut(s) 247, 1969
EcoT22I ATGCAT 2 cut(s) 819, 1062
ErhI CCWWGG 2 cut(s) 247, 1969
FaeI CATG 8 cut(s) 331, 784, 817, 821, 838, 853, 1060, 1264
FaqI GGGAC 3 cut(s) 424, 642, 969
FatI CATG 8 cut(s) 327, 780, 813, 817, 834, 849, 1056, 1260
FauI CCCGC 1 cut(s) 888
FokI GGATG 5 cut(s) 22, 43, 284, 404, 595
FspBI CTAG 5 cut(s) 255, 479, 591, 1097, 1112
GlaI GCGC 1 cut(s) 1035
HaeIII GGCC 8 cut(s) 484, 1410, 1752, 1902, 2043, 2184, 2325, 2466
HapII CCGG 4 cut(s) 301, 1139, 1667, 2708
HhaI GCGC 1 cut(s) 1036
Hin1II CATG 8 cut(s) 331, 784, 817, 821, 838, 853, 1060, 1264
Hin6I GCGC 1 cut(s) 1034
HinP1I GCGC 1 cut(s) 1034
HincII GTYRAC 2 cut(s) 175, 580
HindII GTYRAC 2 cut(s) 175, 580
HindIII AAGCTT 4 cut(s) 1760, 2192, 2333, 2474
HpaII CCGG 4 cut(s) 301, 1139, 1667, 2708
HphI GGTGA 7 cut(s) 33, 125, 331, 1148, 1188, 1656, 1727
Hpy166II GTNNAC 2 cut(s) 175, 580
Hpy8I GTNNAC 2 cut(s) 175, 580
Hpy99I CGWCG 1 cut(s) 2740
HpyCH4III ACNGT 3 cut(s) 1160, 2704, 2770
HpyCH4IV ACGT 2 cut(s) 883, 2735
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpySE526I ACGT 2 cut(s) 883, 2735
Hsp92II CATG 8 cut(s) 331, 784, 817, 821, 838, 853, 1060, 1264
HspAI GCGC 1 cut(s) 1034
LguI GCTCTTC 1 cut(s) 596
LmnI GCTCC 2 cut(s) 313, 1624
Lsp1109I GCAGC 9 cut(s) 151, 496, 980, 1040, 1178, 1618, 2154, 2295, 2627
LweI GCATC 7 cut(s) 1069, 1309, 1792, 1942, 2083, 2224, 2506
MaeI CTAG 5 cut(s) 255, 479, 591, 1097, 1112
MaeII ACGT 2 cut(s) 883, 2735
MaeIII GTNAC 2 cut(s) 689, 2837
MfeI CAATTG 2 cut(s) 1433, 1481
MhlI GDGCHC 1 cut(s) 608
MlyI GAGTC 2 cut(s) 206, 1119
MmeI TCCRAC 4 cut(s) 691, 739, 1602, 2644
Mph1103I ATGCAT 2 cut(s) 819, 1062
MroXI GAANNNNTTC 4 cut(s) 426, 643, 667, 1008
MspA1I CMGCKG 1 cut(s) 1609
MspCI CTTAAG 5 cut(s) 1280, 2069, 2210, 2351, 2492
MspI CCGG 4 cut(s) 301, 1139, 1667, 2708
MspR9I CCNGG 3 cut(s) 990, 1076, 1140
MunI CAATTG 2 cut(s) 1433, 1481
MvaI CCWGG 2 cut(s) 990, 1076
MwoI GCNNNNNNNGC 1 cut(s) 23
NciI CCSGG 1 cut(s) 1140
NlaIII CATG 8 cut(s) 331, 784, 817, 821, 838, 853, 1060, 1264
NlaIV GGNNCC 2 cut(s) 900, 957
NsiI ATGCAT 2 cut(s) 819, 1062
NspI RCATGY 2 cut(s) 817, 1060
NspV TTCGAA 1 cut(s) 787
PaeI GCATGC 2 cut(s) 817, 1060
PciSI GCTCTTC 1 cut(s) 596
PcsI WCGNNNNNNNCGW 1 cut(s) 784
PdmI GAANNNNTTC 4 cut(s) 426, 643, 667, 1008
PleI GAGTC 2 cut(s) 205, 1119
PpsI GAGTC 2 cut(s) 205, 1119
PpuMI RGGWCCY 1 cut(s) 1966
Psp5II RGGWCCY 1 cut(s) 1966
Psp6I CCWGG 2 cut(s) 988, 1074
PspEI GGTNACC 1 cut(s) 2837
PspGI CCWGG 2 cut(s) 988, 1074
PspN4I GGNNCC 2 cut(s) 900, 957
PspPI GGNCC 3 cut(s) 482, 956, 1966
PspPPI RGGWCCY 1 cut(s) 1966
PstI CTGCAG 4 cut(s) 514, 998, 1608, 2617
RsaI GTAC 1 cut(s) 1000
RsaNI GTAC 1 cut(s) 999
SapI GCTCTTC 1 cut(s) 596
Sau96I GGNCC 3 cut(s) 482, 956, 1966
SchI GAGTC 2 cut(s) 206, 1119
ScrFI CCNGG 3 cut(s) 990, 1076, 1140
SduI GDGCHC 1 cut(s) 608
SfaNI GCATC 7 cut(s) 1069, 1309, 1792, 1942, 2083, 2224, 2506
SfcI CTRYAG 4 cut(s) 510, 994, 1604, 2613
SfuI TTCGAA 1 cut(s) 787
SinI GGWCC 2 cut(s) 956, 1966
SphI GCATGC 2 cut(s) 817, 1060
SsiI CCGC 5 cut(s) 290, 445, 895, 1164, 1609
SspI AATATT 1 cut(s) 369
SspMI CTAG 5 cut(s) 255, 479, 591, 1097, 1112
StyD4I CCNGG 3 cut(s) 988, 1074, 1138
StyI CCWWGG 2 cut(s) 247, 1969
TaaI ACNGT 3 cut(s) 1160, 2704, 2770
TaiI ACGT 2 cut(s) 886, 2738
TaqI TCGA 7 cut(s) 787, 1224, 1423, 1547, 1632, 1819, 2738
TaqII GACCGA 1 cut(s) 1905
TatI WGTACW 1 cut(s) 998
TauI GCSGC 1 cut(s) 1612
TseI GCWGC 9 cut(s) 164, 509, 993, 1053, 1166, 1606, 2167, 2308, 2615
TspDTI ATGAA 9 cut(s) 1395, 1887, 2028, 2169, 2310, 2451, 2646, 2715, 2889
Vha464I CTTAAG 5 cut(s) 1280, 2069, 2210, 2351, 2492
VpaK11BI GGWCC 2 cut(s) 956, 1966
XbaI TCTAGA 1 cut(s) 1096
XceI RCATGY 2 cut(s) 817, 1060
XmnI GAANNNNTTC 4 cut(s) 426, 643, 667, 1008
XspI CTAG 5 cut(s) 255, 479, 591, 1097, 1112
Zsp2I ATGCAT 2 cut(s) 819, 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.