pycom15g32450

actin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0028847
Physical Location & Seq
Forward (+)
31453047 .. 31457535
4489 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2892 bp
ATGGATACCAATACAGCCCTTGAAGCCTCCTGTCCATCCTCACCTTCCTCCCCCACATCCTCCTCCTCCTCAATAGAAGATGAGATTTACGATGTGTTCTTGAGCTTTAGTGGTGAAGACACACGCAAAACATTCACAGACCACCTCTACTGGACATTGAAAGCTGCCAGAGTTGACACCTTTATTGACGAGAATGAGTCAATGAGCATAGGGGAAACTATATCAGACAAAGTGAAGCAAGCTATCCAAGGGTCTAGGATCGCTGTCATCATCTTCTCAAGGAGGTATGCGGATTCCATCCGGTGTCTTGAGGAGCTGGTGAAGATCATGGAGTGTAAAAGAACAATGGGGCAAATGGTTTTGCCAATATTCTATGATGTTGATCCTTCGGATGTCAAGAATCAGTCTGGGACTTTCGCAGAAGCATTTCAGAAACACAAAGACCGCTTCCATCAAGTTAAAGATAAGAAAGAAAAGCTAGGGCCGTGGAGAAACGCTCTTACTGAAGCTGCAGATTTGCCCAGTGAGAATTTTGCAAAGACTGACGGGTATCAAGGAGTGTTTATCACGAAAATTGTTGACGAGATCACTAGAAAACTGAAGAGCACATCCTTAGGCGTAGCCACGGGACAAGATGGAATACATTCTCAAGTGCAAGCTGGAATAGATTCCCCCCTACAAGAAATCAGTTACTATACAGATGTTCAAGTTGGATTAGATTCTCGTGTGCAAGAAATTTGTAATTATTTAGATGGTGTTGGAGGATCAAATGATGTTCGCATGATTCGAATCTGTGGTATGGGTGGAATAGGCATGCATGATATGATTCAAGACATGGGCAGAGAAATCATGCTTGCAGAATCCTCGGGCAACCCTGAAGAACGTAGCAGATTGCGGGTTCCTAAAGATGTAGAAGGTGTATTGAGGAATAACTCTGGAACGAGAAAGCTAATGGGACCAAGTTTAGATTTGCATAAACCTGACAAGCCTGGCTGCAGTACAGAATCATTTCAAGATATGCAAATTCTGAGTGCGCTCAAACTCACTGGAAGCTGCATGCATCATTCTACTTGCCTGGCACGATCCCCAGACTTTCTAGAAATCATAGAACTAGAGAAACTGAGACTCAAAGACTTGCCGGGTTTGTCTGTGATTCACCGTTCCGCAGCACACCTGTTTAACCTTAATTATTTATCACCAGACTTCCTGATGGAACTGATAATCGATGACTGCAAGAGTTTGTCCAAGTTTCTCCCTTCCATGATACAACTGAAGAACCTTAAGTATTTATATCTTGAGAACTGCAACTTAACAAATGATGCAATTCCAAAGGATCTTGGGGGTCTATCTTCTTTAGAAGATTTAGATCTAAGTGGCAATGAATTTAGTGGGCTACCTTCCCTCAGTGGCCTTTCCAAACTTCGAGCATTGCAATTGCGTAATTGTAAAAGCCTTCAGTCAATTCCAGATTTACCAAAGCAATTGATAATCTTGGTAGCAAATGGGTGCATTGCTCTGAAAAAAATGCCAGATTTTTCAGATATGTCGAGTATAAGAGAATTGTATCTGAATCACTCCCCCAAACTCACTGAGCCAATAATCCTGCAGCGGCTTGAAAAGTTGGAGCTTCTCGATCTTGGGTATTGTCATCGCCTGAAAACATCACCGGACTTTTCAAAACTCCCAAATCTCAACATTCTGTTTCTCAATGACTGCAAGAGTTTGTCTGAGATTCACCCTTCCCTCAGTGGCCTTTCCAAGCTTCAGTCTTTGTATCTTGAGAACTGCAACTTAACAAATGATGCAATTCCAAAGGATCGATCCCTACTTGGGGGTCTATCTTCTTTAGAAGTTTTAGATCTCAGTGGCAATGAATTTAGTGGGCTACCTTCCCTCAGTGGCCTTTCCGAGCTTCGGTCTTTATATCTTAATAACTGCAACTTAACAAATGATGCAATTCCAAAGGACCTTGGGGGTCTATCTTCTTTAGAAGTTTTGGATCTCAGTGGCAATGAATTTAGTGGGCTACCTTCCCTCAGTGGCCTTTCCGAGCTTTGGTGTTTATATCTTAAGAACTGCAACTTAACAAATGATGCAATTCCGAAGGATCTTAGGGGTCTATCTTCTTTAAAAGTTTTAGATCTCAGTGGCAATGAATTTAGTGGGCTGCCTTCCCTCAGTGGCCTTTCCAAGCTTCGGTGTTTATATCTTAAGAACTGCAACTTAACAAATGATGCAATTCCGAAGGATCTTAGGGGTCTATCTTCTTTAAAAGTTTTAGATCTCAGTGGCAATGAATTTAGTGGGCTGCCTTCCCTCAGTGGCCTTTCCAAGCTTCGGTGTTTATATCTTAAGAACTGCAACTTAACAAATTATGCAATTCCAAAGGATCTTGGGGGTCTATCTTCGTTAAAAGTTTTAGATCTCAGTGGCAATGAATTTAGTGGGCTACCTTCCCTCAGTGGCCTTTCCAAGCTTCGGTGTTTATATCTTAAGAACTGCAACTTAACAAATGATGCAACTCCAAAGGATCTTGGGGGTCTATCTTCTTTAGAAGAAGTGGCAATGATTTTAGTGGGCTACCCTGTGGGAAAGAGGATTTATTCTTCTTCTGCAGCTTCCTCTTCTGATGAACAAGAAGAAGAAGAAGAAGAAGGAGAGGTTGGAGAGGAGGGTGCTATGGAGATTGAGATTAGAGATACTGTGCCGGTCTCTGAAGGGTGTTCATTTACTACGTCGAATGATGATGATTCTTCCAGCACCACCACTGTTTCTCCGAATGAGAGAATTTCGCAGAATGGGAGGCTGAATTGTATTATCACTCACTGGGAGAAGGGTTACCTTCTTGGCAGTGGGTCTTTCGGGTCAGTGTATGAAGCAATTTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 map04011
Pfam Domains
Protein Families
Drf_FH3 Drf_GBD FH2

Protein Analysis

964

Amino Acids

105.54

Weight (kDa)

5.02

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 30 - 204 1.1e-46 TIR domain
TIR_2 PF13676 32 - 130 8.4e-15 TIR domain
LRR_14 PF23598 415 - 495 8.9e-06 Leucine-rich repeat region
LRR_8 PF13855 426 - 482 6.7e-06 Leucine rich repeat
LRR_Zer-1 PF25013 534 - 628 2.7e-06 Zer-1-like, Leucine-rich repeats
LRR_8 PF13855 608 - 648 6.3e-06 Leucine rich repeat
LRR_4 PF12799 615 - 649 9.2e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 615 - 715 1.1e-10 Leucine-rich repeat region
LRR_14 PF23598 665 - 762 8.2e-09 Leucine-rich repeat region
LRR_8 PF13855 705 - 742 7.2e-06 Leucine rich repeat
LRR_14 PF23598 706 - 787 1.1e-08 Leucine-rich repeat region
LRR_8 PF13855 752 - 789 7.2e-06 Leucine rich repeat
LRR_14 PF23598 754 - 858 3.1e-12 Leucine-rich repeat region
LRR_8 PF13855 799 - 836 8.8e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028847)

Species Orthologous Gene IDs
malus_domestica MD07G1102600.v1.1
pyrus_communis pycom15g32450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 290, 445, 895, 1164, 1609
AcuI CTGAAG 7 cut(s) 525, 620, 897, 1292, 1439, 1748, 2736
AfaI GTAC 1 cut(s) 1000
AfiI CCNNNNNNNGG 7 cut(s) 1830, 1831, 1914, 2055, 2196, 2337, 2478
AflII CTTAAG 5 cut(s) 1280, 2069, 2210, 2351, 2492
AgsI TTSAA 7 cut(s) 23, 160, 707, 830, 1013, 1616, 1677
AjnI CCWGG 2 cut(s) 988, 1074
AjuI GAANNNNNNNTTGG 2 cut(s) 2646, 2678
Alw21I GWGCWC 1 cut(s) 608
Alw26I GTCTC 2 cut(s) 1117, 2716
Ama87I CYCGRG 1 cut(s) 865
AoxI GGCC 8 cut(s) 482, 1408, 1750, 1900, 2041, 2182, 2323, 2464
ApeKI GCWGC 9 cut(s) 164, 509, 993, 1053, 1166, 1606, 2167, 2308, 2615
Asp700I GAANNNNTTC 4 cut(s) 426, 643, 667, 1008
AspLEI GCGC 1 cut(s) 1036
AspS9I GGNCC 3 cut(s) 482, 956, 1966
AsuC2I CCSGG 1 cut(s) 1140
AsuHPI GGTGA 7 cut(s) 33, 125, 331, 1148, 1188, 1656, 1727
AsuII TTCGAA 1 cut(s) 787
AvaI CYCGRG 1 cut(s) 865
AvaII GGWCC 2 cut(s) 956, 1966
AxyI CCTNAGG 1 cut(s) 613
BauI CACGAG 1 cut(s) 723
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 608
BbvI GCAGC 9 cut(s) 151, 496, 980, 1040, 1178, 1618, 2154, 2295, 2627
BccI CCATC 6 cut(s) 43, 305, 459, 629, 746, 1204
BceAI ACGGC 1 cut(s) 469
BcgI CGANNNNNNTGC 2 cut(s) 846, 880
BciT130I CCWGG 2 cut(s) 990, 1076
BcnI CCSGG 1 cut(s) 1140
BcoDI GTCTC 2 cut(s) 1117, 2716
BfaI CTAG 5 cut(s) 255, 479, 591, 1097, 1112
BfmI CTRYAG 4 cut(s) 510, 994, 1604, 2613
BfrI CTTAAG 5 cut(s) 1280, 2069, 2210, 2351, 2492
BglII AGATCT 5 cut(s) 1366, 1858, 2140, 2281, 2422
Bme1390I CCNGG 3 cut(s) 990, 1076, 1140
Bme18I GGWCC 2 cut(s) 956, 1966
BmeT110I CYCGRG 1 cut(s) 865
BmgT120I GGNCC 3 cut(s) 482, 956, 1966
BmiI GGNNCC 2 cut(s) 900, 957
BmrFI CCNGG 3 cut(s) 990, 1076, 1140
BmrI ACTGGG 2 cut(s) 516, 2836
BmsI GCATC 7 cut(s) 1069, 1309, 1792, 1942, 2083, 2224, 2506
BmuI ACTGGG 2 cut(s) 516, 2836
BpiI GAAGAC 1 cut(s) 123
BplI GAGNNNNNCTC 2 cut(s) 481, 513
Bpu14I TTCGAA 1 cut(s) 787
BpuEI CTTGAG 6 cut(s) 121, 262, 329, 633, 1316, 1799
BpuMI CCSGG 1 cut(s) 1140
Bsa29I ATCGAT 2 cut(s) 1224, 1819
BsaBI GATNNNNATC 3 cut(s) 381, 788, 1365
BsaI GGTCTC 1 cut(s) 2716
BsaJI CCNNGG 5 cut(s) 247, 485, 624, 864, 1969
BsaWI WCCGGW 2 cut(s) 300, 1666
BsaXI ACNNNNNCTCC 2 cut(s) 2758, 2788
Bsc4I CCNNNNNNNGG 7 cut(s) 1830, 1831, 1914, 2055, 2196, 2337, 2478
Bse118I RCCGGY 1 cut(s) 2707
Bse1I ACTGG 4 cut(s) 155, 522, 1051, 2831
Bse21I CCTNAGG 1 cut(s) 613
Bse3DI GCAATG 9 cut(s) 1384, 1427, 1509, 1876, 2017, 2158, 2299, 2440, 2571
Bse8I GATNNNNATC 3 cut(s) 381, 788, 1365
BseBI CCWGG 2 cut(s) 990, 1076
BseCI ATCGAT 2 cut(s) 1224, 1819
BseDI CCNNGG 5 cut(s) 247, 485, 624, 864, 1969
BseGI GGATG 5 cut(s) 35, 56, 297, 397, 608
BseJI GATNNNNATC 3 cut(s) 381, 788, 1365
BseLI CCNNNNNNNGG 7 cut(s) 1830, 1831, 1914, 2055, 2196, 2337, 2478
BseMI GCAATG 9 cut(s) 1384, 1427, 1509, 1876, 2017, 2158, 2299, 2440, 2571
BseNI ACTGG 4 cut(s) 155, 522, 1051, 2831
BseRI GAGGAG 5 cut(s) 52, 55, 58, 326, 2684
BseXI GCAGC 9 cut(s) 151, 496, 980, 1040, 1178, 1618, 2154, 2295, 2627
BshFI GGCC 8 cut(s) 484, 1410, 1752, 1902, 2043, 2184, 2325, 2466
BshVI ATCGAT 2 cut(s) 1224, 1819
BsiHKAI GWGCWC 1 cut(s) 608
BsiHKCI CYCGRG 1 cut(s) 865
BsiSI CCGG 4 cut(s) 301, 1139, 1667, 2708
BslFI GGGAC 3 cut(s) 424, 642, 969
BslI CCNNNNNNNGG 7 cut(s) 1830, 1831, 1914, 2055, 2196, 2337, 2478
BsmAI GTCTC 2 cut(s) 1117, 2716
BsmFI GGGAC 3 cut(s) 424, 642, 969
BsnI GGCC 8 cut(s) 484, 1410, 1752, 1902, 2043, 2184, 2325, 2466
Bso31I GGTCTC 1 cut(s) 2716
BsoBI CYCGRG 1 cut(s) 865
Bsp119I TTCGAA 1 cut(s) 787
Bsp1286I GDGCHC 1 cut(s) 608
BspACI CCGC 5 cut(s) 290, 445, 895, 1164, 1609
BspANI GGCC 8 cut(s) 484, 1410, 1752, 1902, 2043, 2184, 2325, 2466
BspDI ATCGAT 2 cut(s) 1224, 1819
BspLI GGNNCC 2 cut(s) 900, 957
BspMAI CTGCAG 4 cut(s) 514, 998, 1608, 2617
BspQI GCTCTTC 1 cut(s) 596
BspT104I TTCGAA 1 cut(s) 787
BspTI CTTAAG 5 cut(s) 1280, 2069, 2210, 2351, 2492
BspTNI GGTCTC 1 cut(s) 2716
BsrDI GCAATG 9 cut(s) 1384, 1427, 1509, 1876, 2017, 2158, 2299, 2440, 2571
BsrFI RCCGGY 1 cut(s) 2707
BsrI ACTGG 4 cut(s) 155, 522, 1051, 2831
BssAI RCCGGY 1 cut(s) 2707
BssECI CCNNGG 5 cut(s) 247, 485, 624, 864, 1969
BssSI CACGAG 1 cut(s) 723
BssT1I CCWWGG 2 cut(s) 247, 1969
Bst2BI CACGAG 1 cut(s) 723
Bst2UI CCWGG 2 cut(s) 990, 1076
Bst4CI ACNGT 3 cut(s) 1160, 2704, 2770
Bst6I CTCTTC 2 cut(s) 596, 2629
BstAFI CTTAAG 5 cut(s) 1280, 2069, 2210, 2351, 2492
BstBI TTCGAA 1 cut(s) 787
BstC8I GCNNGC 5 cut(s) 240, 657, 815, 855, 1058
BstDSI CCRYGG 2 cut(s) 485, 624
BstEII GGTNACC 1 cut(s) 2837
BstF5I GGATG 5 cut(s) 35, 56, 297, 397, 608
BstHHI GCGC 1 cut(s) 1036
BstMAI GTCTC 2 cut(s) 1117, 2716
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 2 cut(s) 990, 1076
BstNSI RCATGY 2 cut(s) 817, 1060
BstPI GGTNACC 1 cut(s) 2837
BstSCI CCNGG 3 cut(s) 988, 1074, 1138
BstSFI CTRYAG 4 cut(s) 510, 994, 1604, 2613
BstV1I GCAGC 9 cut(s) 151, 496, 980, 1040, 1178, 1618, 2154, 2295, 2627
BstV2I GAAGAC 1 cut(s) 123
Bsu15I ATCGAT 2 cut(s) 1224, 1819
Bsu36I CCTNAGG 1 cut(s) 613
BsuRI GGCC 8 cut(s) 484, 1410, 1752, 1902, 2043, 2184, 2325, 2466
BsuTUI ATCGAT 2 cut(s) 1224, 1819
BtgI CCRYGG 2 cut(s) 485, 624
BtgZI GCGATG 1 cut(s) 1634
BtsCI GGATG 5 cut(s) 35, 56, 297, 397, 608
BtsI GCAGTG 1 cut(s) 2857
Cac8I GCNNGC 5 cut(s) 240, 657, 815, 855, 1058
CfoI GCGC 1 cut(s) 1036
Cfr10I RCCGGY 1 cut(s) 2707
Cfr13I GGNCC 3 cut(s) 482, 956, 1966
ClaI ATCGAT 2 cut(s) 1224, 1819
Csp6I GTAC 1 cut(s) 999
CviAII CATG 8 cut(s) 328, 781, 814, 818, 835, 850, 1057, 1261
CviQI GTAC 1 cut(s) 999
DraI TTTAAA 2 cut(s) 2130, 2271
Eam1104I CTCTTC 2 cut(s) 596, 2629
EarI CTCTTC 2 cut(s) 596, 2629
Eco130I CCWWGG 2 cut(s) 247, 1969
Eco31I GGTCTC 1 cut(s) 2716
Eco47I GGWCC 2 cut(s) 956, 1966
Eco57I CTGAAG 7 cut(s) 525, 620, 897, 1292, 1439, 1748, 2736
Eco81I CCTNAGG 1 cut(s) 613
Eco88I CYCGRG 1 cut(s) 865
Eco91I GGTNACC 1 cut(s) 2837
EcoO109I RGGNCCY 1 cut(s) 1966
EcoO65I GGTNACC 1 cut(s) 2837
EcoRII CCWGG 2 cut(s) 988, 1074
EcoT14I CCWWGG 2 cut(s) 247, 1969
EcoT22I ATGCAT 2 cut(s) 819, 1062
ErhI CCWWGG 2 cut(s) 247, 1969
FaeI CATG 8 cut(s) 331, 784, 817, 821, 838, 853, 1060, 1264
FaqI GGGAC 3 cut(s) 424, 642, 969
FatI CATG 8 cut(s) 327, 780, 813, 817, 834, 849, 1056, 1260
FauI CCCGC 1 cut(s) 888
FokI GGATG 5 cut(s) 22, 43, 284, 404, 595
FspBI CTAG 5 cut(s) 255, 479, 591, 1097, 1112
GlaI GCGC 1 cut(s) 1035
HaeIII GGCC 8 cut(s) 484, 1410, 1752, 1902, 2043, 2184, 2325, 2466
HapII CCGG 4 cut(s) 301, 1139, 1667, 2708
HhaI GCGC 1 cut(s) 1036
Hin1II CATG 8 cut(s) 331, 784, 817, 821, 838, 853, 1060, 1264
Hin6I GCGC 1 cut(s) 1034
HinP1I GCGC 1 cut(s) 1034
HincII GTYRAC 2 cut(s) 175, 580
HindII GTYRAC 2 cut(s) 175, 580
HindIII AAGCTT 4 cut(s) 1760, 2192, 2333, 2474
HpaII CCGG 4 cut(s) 301, 1139, 1667, 2708
HphI GGTGA 7 cut(s) 33, 125, 331, 1148, 1188, 1656, 1727
Hpy166II GTNNAC 2 cut(s) 175, 580
Hpy8I GTNNAC 2 cut(s) 175, 580
Hpy99I CGWCG 1 cut(s) 2740
HpyCH4III ACNGT 3 cut(s) 1160, 2704, 2770
HpyCH4IV ACGT 2 cut(s) 883, 2735
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpySE526I ACGT 2 cut(s) 883, 2735
Hsp92II CATG 8 cut(s) 331, 784, 817, 821, 838, 853, 1060, 1264
HspAI GCGC 1 cut(s) 1034
LguI GCTCTTC 1 cut(s) 596
LmnI GCTCC 2 cut(s) 313, 1624
Lsp1109I GCAGC 9 cut(s) 151, 496, 980, 1040, 1178, 1618, 2154, 2295, 2627
LweI GCATC 7 cut(s) 1069, 1309, 1792, 1942, 2083, 2224, 2506
MaeI CTAG 5 cut(s) 255, 479, 591, 1097, 1112
MaeII ACGT 2 cut(s) 883, 2735
MaeIII GTNAC 2 cut(s) 689, 2837
MfeI CAATTG 2 cut(s) 1433, 1481
MhlI GDGCHC 1 cut(s) 608
MlyI GAGTC 2 cut(s) 206, 1119
MmeI TCCRAC 4 cut(s) 691, 739, 1602, 2644
Mph1103I ATGCAT 2 cut(s) 819, 1062
MroXI GAANNNNTTC 4 cut(s) 426, 643, 667, 1008
MspA1I CMGCKG 1 cut(s) 1609
MspCI CTTAAG 5 cut(s) 1280, 2069, 2210, 2351, 2492
MspI CCGG 4 cut(s) 301, 1139, 1667, 2708
MspR9I CCNGG 3 cut(s) 990, 1076, 1140
MunI CAATTG 2 cut(s) 1433, 1481
MvaI CCWGG 2 cut(s) 990, 1076
MwoI GCNNNNNNNGC 1 cut(s) 23
NciI CCSGG 1 cut(s) 1140
NlaIII CATG 8 cut(s) 331, 784, 817, 821, 838, 853, 1060, 1264
NlaIV GGNNCC 2 cut(s) 900, 957
NsiI ATGCAT 2 cut(s) 819, 1062
NspI RCATGY 2 cut(s) 817, 1060
NspV TTCGAA 1 cut(s) 787
PaeI GCATGC 2 cut(s) 817, 1060
PciSI GCTCTTC 1 cut(s) 596
PcsI WCGNNNNNNNCGW 1 cut(s) 784
PdmI GAANNNNTTC 4 cut(s) 426, 643, 667, 1008
PleI GAGTC 2 cut(s) 205, 1119
PpsI GAGTC 2 cut(s) 205, 1119
PpuMI RGGWCCY 1 cut(s) 1966
Psp5II RGGWCCY 1 cut(s) 1966
Psp6I CCWGG 2 cut(s) 988, 1074
PspEI GGTNACC 1 cut(s) 2837
PspGI CCWGG 2 cut(s) 988, 1074
PspN4I GGNNCC 2 cut(s) 900, 957
PspPI GGNCC 3 cut(s) 482, 956, 1966
PspPPI RGGWCCY 1 cut(s) 1966
PstI CTGCAG 4 cut(s) 514, 998, 1608, 2617
RsaI GTAC 1 cut(s) 1000
RsaNI GTAC 1 cut(s) 999
SapI GCTCTTC 1 cut(s) 596
Sau96I GGNCC 3 cut(s) 482, 956, 1966
SchI GAGTC 2 cut(s) 206, 1119
ScrFI CCNGG 3 cut(s) 990, 1076, 1140
SduI GDGCHC 1 cut(s) 608
SfaNI GCATC 7 cut(s) 1069, 1309, 1792, 1942, 2083, 2224, 2506
SfcI CTRYAG 4 cut(s) 510, 994, 1604, 2613
SfuI TTCGAA 1 cut(s) 787
SinI GGWCC 2 cut(s) 956, 1966
SphI GCATGC 2 cut(s) 817, 1060
SsiI CCGC 5 cut(s) 290, 445, 895, 1164, 1609
SspI AATATT 1 cut(s) 369
SspMI CTAG 5 cut(s) 255, 479, 591, 1097, 1112
StyD4I CCNGG 3 cut(s) 988, 1074, 1138
StyI CCWWGG 2 cut(s) 247, 1969
TaaI ACNGT 3 cut(s) 1160, 2704, 2770
TaiI ACGT 2 cut(s) 886, 2738
TaqI TCGA 7 cut(s) 787, 1224, 1423, 1547, 1632, 1819, 2738
TaqII GACCGA 1 cut(s) 1905
TatI WGTACW 1 cut(s) 998
TauI GCSGC 1 cut(s) 1612
TseI GCWGC 9 cut(s) 164, 509, 993, 1053, 1166, 1606, 2167, 2308, 2615
TspDTI ATGAA 9 cut(s) 1395, 1887, 2028, 2169, 2310, 2451, 2646, 2715, 2889
Vha464I CTTAAG 5 cut(s) 1280, 2069, 2210, 2351, 2492
VpaK11BI GGWCC 2 cut(s) 956, 1966
XbaI TCTAGA 1 cut(s) 1096
XceI RCATGY 2 cut(s) 817, 1060
XmnI GAANNNNTTC 4 cut(s) 426, 643, 667, 1008
XspI CTAG 5 cut(s) 255, 479, 591, 1097, 1112
Zsp2I ATGCAT 2 cut(s) 819, 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.