pycom15g34520

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0024399
Physical Location & Seq
Forward (+)
34315176 .. 34316173
998 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 444 bp
ATGACCCGACTCTATCTTGCTTCTCACAAGGTTTGTTTACGGTGGAGAAGCGAAGATCGATCGACTTTGAGAAGATTATTTCCAGATATGGATGATAAGAAAAGCCTTGCCTCTGCAGAGGAAAGGGCCAAAGTTGAAGGCAAGGCTTCTTTGTTAGCCGCAGTTGACTATTATGTGAGCATGCAAAGCGACATCTTCATTTCTGCTTCTCCCGGAAATATGCACAATGCACTGGTGGGTCACCGGGCTTATATGAACTTGAAGACTATTAGACCAAACATGGCATTGTTGGGCAAGCTTTTCGTGAATAAGAGCATGGAATGGTCTGATTTCCAACGCGCGGTATTGGATGGACACAAAACTAGACAGGGGCAGATGAGGCTGAGGAAGGAGAAGCAGTCGATCTATACATACCCTGCTCCTGATTGCATGTGTCAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

148

Amino Acids

16.91

Weight (kDa)

9.68

Isoelectric Point (pI)

60.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 2 - 85 2.4e-12 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024399)

Species Orthologous Gene IDs
malus_domestica MD08G1197600.v1.1
pyrus_communis pycom08g16990 pycom15g34520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 339, 341
AciI CCGC 2 cut(s) 159, 341
AfiI CCNNNNNNNGG 1 cut(s) 340
AgsI TTSAA 2 cut(s) 137, 262
AluBI AGCT 1 cut(s) 298
AluI AGCT 1 cut(s) 298
AoxI GGCC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 126
AsuC2I CCSGG 2 cut(s) 213, 245
AsuHPI GGTGA 1 cut(s) 233
BbsI GAAGAC 1 cut(s) 269
BbvCI CCTCAGC 1 cut(s) 383
BccI CCATC 1 cut(s) 344
BcgI CGANNNNNNTGC 2 cut(s) 283, 317
BcnI CCSGG 2 cut(s) 213, 245
BfaI CTAG 1 cut(s) 363
BfmI CTRYAG 1 cut(s) 114
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme1390I CCNGG 2 cut(s) 213, 245
BmgT120I GGNCC 1 cut(s) 126
BmrFI CCNGG 2 cut(s) 213, 245
BpiI GAAGAC 1 cut(s) 269
Bpu10I CCTNAGC 1 cut(s) 383
BpuMI CCSGG 2 cut(s) 213, 245
Bsa29I ATCGAT 1 cut(s) 58
BsaXI ACNNNNNCTCC 2 cut(s) 383, 413
Bsc4I CCNNNNNNNGG 1 cut(s) 340
Bse1I ACTGG 1 cut(s) 237
BseCI ATCGAT 1 cut(s) 58
BseGI GGATG 2 cut(s) 97, 355
BseLI CCNNNNNNNGG 1 cut(s) 340
BseMII CTCAG 1 cut(s) 374
BseNI ACTGG 1 cut(s) 237
Bsh1236I CGCG 2 cut(s) 339, 341
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 1 cut(s) 128
BshVI ATCGAT 1 cut(s) 58
BsiEI CGRYCG 1 cut(s) 62
BsiSI CCGG 2 cut(s) 213, 244
BslI CCNNNNNNNGG 1 cut(s) 340
BsnI GGCC 1 cut(s) 128
Bsp143I GATC 3 cut(s) 55, 59, 402
BspACI CCGC 2 cut(s) 159, 341
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 1 cut(s) 375
BspDI ATCGAT 1 cut(s) 58
BspFNI CGCG 2 cut(s) 339, 341
BspMAI CTGCAG 1 cut(s) 118
BsrI ACTGG 1 cut(s) 237
BssMI GATC 3 cut(s) 55, 59, 402
Bst4CI ACNGT 1 cut(s) 42
BstC8I GCNNGC 2 cut(s) 182, 296
BstDEI CTNAG 1 cut(s) 383
BstEII GGTNACC 1 cut(s) 239
BstF5I GGATG 2 cut(s) 97, 355
BstFNI CGCG 2 cut(s) 339, 341
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 3 cut(s) 58, 62, 405
BstMBI GATC 3 cut(s) 55, 59, 402
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 2 cut(s) 186, 379
BstNSI RCATGY 2 cut(s) 184, 433
BstPI GGTNACC 1 cut(s) 239
BstSCI CCNGG 2 cut(s) 211, 243
BstSFI CTRYAG 1 cut(s) 114
BstUI CGCG 2 cut(s) 339, 341
BstV2I GAAGAC 1 cut(s) 269
Bsu15I ATCGAT 1 cut(s) 58
BsuRI GGCC 1 cut(s) 128
BsuTUI ATCGAT 1 cut(s) 58
BtsCI GGATG 2 cut(s) 97, 355
BtsIMutI CAGTG 1 cut(s) 230
Cac8I GCNNGC 2 cut(s) 182, 296
CfoI GCGC 1 cut(s) 341
Cfr13I GGNCC 1 cut(s) 126
ClaI ATCGAT 1 cut(s) 58
CviAII CATG 4 cut(s) 181, 280, 316, 430
CviJI RGCY 7 cut(s) 105, 128, 146, 158, 248, 298, 382
CviKI_1 RGCY 7 cut(s) 105, 128, 146, 158, 248, 298, 382
DdeI CTNAG 1 cut(s) 383
DpnI GATC 3 cut(s) 57, 61, 404
DpnII GATC 3 cut(s) 55, 59, 402
Eco91I GGTNACC 1 cut(s) 239
EcoO65I GGTNACC 1 cut(s) 239
FaeI CATG 4 cut(s) 184, 283, 319, 433
FatI CATG 4 cut(s) 180, 279, 315, 429
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 2 cut(s) 104, 362
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 363
GlaI GCGC 1 cut(s) 340
GluI GCNGC 1 cut(s) 159
HaeIII GGCC 1 cut(s) 128
HapII CCGG 2 cut(s) 213, 244
HhaI GCGC 1 cut(s) 341
Hin1II CATG 4 cut(s) 184, 283, 319, 433
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 1 cut(s) 9
HpaII CCGG 2 cut(s) 213, 244
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 2 cut(s) 38, 166
Hpy188I TCNGA 1 cut(s) 328
Hpy188III TCNNGA 3 cut(s) 83, 304, 422
Hpy8I GTNNAC 2 cut(s) 38, 166
HpyAV CCTTC 2 cut(s) 131, 382
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4V TGCA 5 cut(s) 116, 184, 223, 230, 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 379
HpyF3I CTNAG 1 cut(s) 383
Hsp92II CATG 4 cut(s) 184, 283, 319, 433
HspAI GCGC 1 cut(s) 339
Kzo9I GATC 3 cut(s) 55, 59, 402
LmnI GCTCC 1 cut(s) 424
LpnPI CCDG 7 cut(s) 96, 218, 226, 257, 353, 429, 435
MaeI CTAG 1 cut(s) 363
MaeIII GTNAC 1 cut(s) 239
MalI GATC 3 cut(s) 57, 61, 404
MboI GATC 3 cut(s) 55, 59, 402
MboII GAAGA 4 cut(s) 65, 84, 187, 274
MlyI GAGTC 1 cut(s) 3
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 4 cut(s) 112, 121, 372, 378
MspI CCGG 2 cut(s) 213, 244
MspR9I CCNGG 2 cut(s) 213, 245
MvnI CGCG 2 cut(s) 339, 341
MwoI GCNNNNNNNGC 2 cut(s) 186, 379
NciI CCSGG 2 cut(s) 213, 245
NdeII GATC 3 cut(s) 55, 59, 402
NlaIII CATG 4 cut(s) 184, 283, 319, 433
NmuCI GTSAC 1 cut(s) 239
NspI RCATGY 2 cut(s) 184, 433
PaeI GCATGC 1 cut(s) 184
PfoI TCCNGGA 1 cut(s) 211
PkrI GCNGC 1 cut(s) 160
Ple19I CGATCG 1 cut(s) 62
PleI GAGTC 1 cut(s) 3
PpsI GAGTC 1 cut(s) 3
PspEI GGTNACC 1 cut(s) 239
PspPI GGNCC 1 cut(s) 126
PstI CTGCAG 1 cut(s) 118
PvuI CGATCG 1 cut(s) 62
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 3 cut(s) 55, 59, 402
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 1 cut(s) 3
ScrFI CCNGG 2 cut(s) 213, 245
SetI ASST 2 cut(s) 33, 300
SfcI CTRYAG 1 cut(s) 114
SphI GCATGC 1 cut(s) 184
SsiI CCGC 2 cut(s) 159, 341
SspMI CTAG 1 cut(s) 363
StyD4I CCNGG 2 cut(s) 211, 243
TaaI ACNGT 1 cut(s) 42
TaqI TCGA 3 cut(s) 58, 62, 401
TauI GCSGC 1 cut(s) 161
TscAI CASTG 1 cut(s) 237
TseFI GTSAC 1 cut(s) 239
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 2 cut(s) 187, 269
TspRI CASTG 1 cut(s) 237
XceI RCATGY 2 cut(s) 184, 433
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.