pycom15g38760
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0028635
Physical Location & Seq
Reverse (-)
38517393 .. 38518834
1442 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g38760.2

Sequence Viewer

Length: 465 bp
ATGTTGTCGAATTTGAGGTCAATTCTTGGAAGTTTTATCTTAATTGTAGAGTCCAATTTTGTGGGCACCACTACTGGTTCTACAAATACTGAAGGAATATTGAAGAAAGATGAAGGGAATGGATTTTGTAGTGGCATCATAGATCAGTACATTGTTCCATTAAGCTTGGTAGGGAGGTGGGGAAGTGCCATGGGCATGCAGCCAAAGAATACGGATAAAGCATCTTTATTGAAGGAGCAGCACCTTAGATACTTTACCCCGAGGGAGTTATGCATTGCTGGGGAACAGTTTGAGTGTGGTTGCTCCGTTACTCAAATACTTATTTGTCGGAACATCGTGATTATTCTAAAGACACCCAAATGCAGTAGATGCAGATCTCACCCACAGCTATACGTTTGGCTCACTATATCAACCACTAACCTTAAACTTCTCTCCCCGGGTATCTCTGCTAACTCTAACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

155

Amino Acids

16.94

Weight (kDa)

8.92

Isoelectric Point (pI)

29.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028635)

Species Orthologous Gene IDs
malus_domestica MD08G1143500.v1.1
pyrus_communis pycom15g38760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 65
AcsI RAATTY 1 cut(s) 10
AcuI CTGAAG 1 cut(s) 111
AfaI GTAC 1 cut(s) 149
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 2 cut(s) 103, 232
AluBI AGCT 2 cut(s) 165, 388
AluI AGCT 2 cut(s) 165, 388
Ama87I CYCGRG 2 cut(s) 259, 436
ApeKI GCWGC 2 cut(s) 199, 238
ApoI RAATTY 1 cut(s) 10
ArsI GACNNNNNNTTYG 1 cut(s) 34
AsuC2I CCSGG 2 cut(s) 437, 438
AsuHPI GGTGA 1 cut(s) 371
AvaI CYCGRG 2 cut(s) 259, 436
BaeGI GKGCMC 1 cut(s) 68
BanI GGYRCC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 211, 250
BcnI CCSGG 2 cut(s) 437, 438
BglII AGATCT 1 cut(s) 374
BisI GCNGC 2 cut(s) 200, 239
BlsI GCNGC 2 cut(s) 201, 240
Bme1390I CCNGG 2 cut(s) 437, 438
BmeT110I CYCGRG 2 cut(s) 259, 436
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 2 cut(s) 437, 438
BmsI GCATC 3 cut(s) 144, 230, 359
BpuMI CCSGG 2 cut(s) 437, 438
BsaBI GATNNNNATC 1 cut(s) 373
BsaJI CCNNGG 4 cut(s) 189, 260, 435, 436
Bse1I ACTGG 1 cut(s) 79
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 373
BseDI CCNNGG 4 cut(s) 189, 260, 435, 436
BseJI GATNNNNATC 1 cut(s) 373
BseMI GCAATG 1 cut(s) 273
BseNI ACTGG 1 cut(s) 79
BseSI GKGCMC 1 cut(s) 68
BseXI GCAGC 2 cut(s) 211, 250
BseYI CCCAGC 1 cut(s) 278
BshNI GGYRCC 1 cut(s) 65
BsiHKCI CYCGRG 2 cut(s) 259, 436
BsiSI CCGG 1 cut(s) 437
BsoBI CYCGRG 2 cut(s) 259, 436
Bsp1286I GDGCHC 1 cut(s) 68
Bsp143I GATC 2 cut(s) 142, 374
Bsp19I CCATGG 1 cut(s) 189
BspLI GGNNCC 1 cut(s) 67
BspT107I GGYRCC 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 273
BsrI ACTGG 1 cut(s) 79
BssECI CCNNGG 4 cut(s) 189, 260, 435, 436
BssMI GATC 2 cut(s) 142, 374
BssT1I CCWWGG 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 288
BstAPI GCANNNNNTGC 1 cut(s) 369
BstC8I GCNNGC 1 cut(s) 197
BstDEI CTNAG 1 cut(s) 245
BstDSI CCRYGG 1 cut(s) 189
BstKTI GATC 2 cut(s) 145, 377
BstMBI GATC 2 cut(s) 142, 374
BstMWI GCNNNNNNNGC 1 cut(s) 369
BstNSI RCATGY 2 cut(s) 199, 463
BstSCI CCNGG 2 cut(s) 435, 436
BstSLI GKGCMC 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 211, 250
BstX2I RGATCY 1 cut(s) 374
BstXI CCANNNNNNTGG 1 cut(s) 61
BstYI RGATCY 1 cut(s) 374
BtgI CCRYGG 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 197
Cfr9I CCCGGG 1 cut(s) 436
Csp6I GTAC 1 cut(s) 148
CviAII CATG 3 cut(s) 190, 196, 460
CviJI RGCY 4 cut(s) 165, 202, 388, 400
CviKI_1 RGCY 4 cut(s) 165, 202, 388, 400
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 1 cut(s) 245
DpnI GATC 2 cut(s) 144, 376
DpnII GATC 2 cut(s) 142, 374
Eco130I CCWWGG 1 cut(s) 189
Eco57I CTGAAG 1 cut(s) 111
Eco88I CYCGRG 2 cut(s) 259, 436
EcoT14I CCWWGG 1 cut(s) 189
EcoT22I ATGCAT 1 cut(s) 275
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 3 cut(s) 193, 199, 463
FaiI YATR 7 cut(s) 140, 191, 197, 271, 391, 407, 461
FatI CATG 3 cut(s) 189, 195, 459
Fnu4HI GCNGC 2 cut(s) 200, 239
Fsp4HI GCNGC 2 cut(s) 200, 239
GluI GCNGC 2 cut(s) 200, 239
GsaI CCCAGC 1 cut(s) 282
HapII CCGG 1 cut(s) 437
Hin1II CATG 3 cut(s) 193, 199, 463
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 1 cut(s) 50
HpaII CCGG 1 cut(s) 437
HphI GGTGA 1 cut(s) 371
Hpy188I TCNGA 1 cut(s) 330
Hpy188III TCNNGA 1 cut(s) 337
HpyAV CCTTC 3 cut(s) 86, 107, 226
HpyCH4III ACNGT 1 cut(s) 288
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 4 cut(s) 199, 273, 363, 372
HpyF10VI GCNNNNNNNGC 1 cut(s) 369
HpyF3I CTNAG 1 cut(s) 245
HpySE526I ACGT 1 cut(s) 393
Hsp92II CATG 3 cut(s) 193, 199, 463
Kzo9I GATC 2 cut(s) 142, 374
LmnI GCTCC 2 cut(s) 235, 308
LpnPI CCDG 3 cut(s) 60, 264, 450
Lsp1109I GCAGC 2 cut(s) 211, 250
LweI GCATC 3 cut(s) 144, 230, 359
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 1 cut(s) 307
MalI GATC 2 cut(s) 144, 376
MboI GATC 2 cut(s) 142, 374
MboII GAAGA 1 cut(s) 115
MflI RGATCY 1 cut(s) 374
MhlI GDGCHC 1 cut(s) 68
MluCI AATT 4 cut(s) 10, 21, 42, 55
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 308
MnlI CCTC 3 cut(s) 9, 168, 255
Mph1103I ATGCAT 1 cut(s) 275
MseI TTAA 3 cut(s) 41, 161, 423
MslI CAYNNNNRTG 2 cut(s) 194, 358
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 2 cut(s) 437, 438
MwoI GCNNNNNNNGC 1 cut(s) 369
NciI CCSGG 2 cut(s) 437, 438
NcoI CCATGG 1 cut(s) 189
NdeII GATC 2 cut(s) 142, 374
NlaIII CATG 3 cut(s) 193, 199, 463
NlaIV GGNNCC 1 cut(s) 67
NsiI ATGCAT 1 cut(s) 275
NspI RCATGY 2 cut(s) 199, 463
PaeI GCATGC 1 cut(s) 199
PciI ACATGT 1 cut(s) 459
PkrI GCNGC 2 cut(s) 201, 240
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
PscI ACATGT 1 cut(s) 459
PspFI CCCAGC 1 cut(s) 278
PspN4I GGNNCC 1 cut(s) 67
PsuI RGATCY 1 cut(s) 374
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
RseI CAYNNNNRTG 2 cut(s) 194, 358
SaqAI TTAA 3 cut(s) 41, 161, 423
SatI GCNGC 2 cut(s) 200, 239
Sau3AI GATC 2 cut(s) 142, 374
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 2 cut(s) 437, 438
SduI GDGCHC 1 cut(s) 68
SetI ASST 7 cut(s) 20, 167, 179, 246, 390, 396, 423
SfaNI GCATC 3 cut(s) 144, 230, 359
SmaI CCCGGG 1 cut(s) 438
SmiMI CAYNNNNRTG 2 cut(s) 194, 358
SphI GCATGC 1 cut(s) 199
Sse9I AATT 4 cut(s) 10, 21, 42, 55
SspI AATATT 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 435, 436
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 1 cut(s) 288
TaiI ACGT 1 cut(s) 396
TaqI TCGA 1 cut(s) 8
TasI AATT 4 cut(s) 10, 21, 42, 55
TatI WGTACW 1 cut(s) 147
Tru1I TTAA 3 cut(s) 41, 161, 423
Tru9I TTAA 3 cut(s) 41, 161, 423
TseI GCWGC 2 cut(s) 199, 238
TspDTI ATGAA 1 cut(s) 126
TspGWI ACGGA 2 cut(s) 227, 295
TspMI CCCGGG 1 cut(s) 436
XapI RAATTY 1 cut(s) 10
XceI RCATGY 2 cut(s) 199, 463
XmaI CCCGGG 1 cut(s) 436
Zsp2I ATGCAT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.