pycom16g01370

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0029472
Physical Location & Seq
Forward (+)
878661 .. 880032
1372 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 921 bp
ATGAGTAAAGAGGAAACAAGAAGAAGAATCAAAGAAATTGGTCTAGTGCTGATGGGTGGGAAGACGAAGATGCAGCGCCACCACTCAGTGGCTGTGAGGTTGAATGAGCAAATGGGGGCCAAAAAAGGCTACACCTTTGTGCAGGCACACAGGGCTTGGTTCCCAAAACTGTTCCTTCTTTGGGTTGGTGTGATGCTTCTCTTGGGCTGGTTAACCTACTCTTGCATGGATGCTGATAACAAAGTGAGAAGGGTAGAAGTTCTTGGTAGCATGTGTGATCGATCGGCAAGAATGCTGCAGGATCAGTTCAGTGTTAGTGTTAATCATGTGCATGCCCTTGCAATCCTTGTTTCCACATTCCATTACTACAAGAACCCTTCTGCAATTGATCAGGAAACATTTGCTGAATACACTGCCAGAACCGCCTTCGAGAGGCCTCTTTTGAGTGGGGTGGCCTATGCACAGAGAGTGCTTGATTCGGATAGGGAAAGGTTTGAAAACCAACATGGATGGACAATAAAGACAATGGGGAAGGAACCATCGCCCAACCGAGATGAGTATGCGCCTGTAATTTTTTCACAGGAAACCGTCTCTTACATTGAGTCACTTGATATGATGTCTGGGGAGGAGGACAGAGAAAACATTTTGAGGGCTAGAGCTACTGGAAAAGCTGTTCTAACAAGCCCATTTAGGCTGCTGGGTTCTCATCATCTTGGTGTTGTATTGACTTTCCCCGTTTACAAATCGAAGCTGCCTCCAAACCCAACTGTGGAGCAGCGCATTCAGGCGGCTGTTGGTCCATGTACCTTGGTGGTGCCTTTGATGTTGAGTCCCTTGTGGAGAATTTGCTTGGCCAACTTGCGGGGAATCAAGCCATTCAGGTTTATGTGTATGATGTTACAAACACTTCTGATCCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004721 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0007275 GO:0008150 GO:0008152 GO:0008272 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009743 GO:0009744 GO:0009755 GO:0009784 GO:0009790 GO:0009884 GO:0009885 GO:0009987 GO:0009991 GO:0010015 GO:0010029 GO:0010033 GO:0010035 GO:0010086 GO:0012505 GO:0015698 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016787 GO:0016788 GO:0016791 GO:0018106 GO:0018193 GO:0018202 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023052 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032870 GO:0033500 GO:0033554 GO:0034285 GO:0035556 GO:0036211 GO:0038023 GO:0042221 GO:0042578 GO:0042592 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048509 GO:0048580 GO:0048598 GO:0048731 GO:0048831 GO:0048856 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071470 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0072348 GO:0098542 GO:0099402 GO:0104004 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

307

Amino Acids

34.92

Weight (kDa)

9.53

Isoelectric Point (pI)

55.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 128 - 266 1.4e-30 CHASE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029472)

Species Orthologous Gene IDs
pyrus_communis pycom16g01370
rosa_multiflora Rmu_co8384703.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 814
AccB7I CCANNNNNTGG 1 cut(s) 88
AciI CCGC 3 cut(s) 423, 788, 862
AclWI GGATC 2 cut(s) 309, 907
AcoI YGGCCR 1 cut(s) 852
AcsI RAATTY 1 cut(s) 843
AdeI CACNNNGTG 1 cut(s) 88
AfaI GTAC 1 cut(s) 805
AfiI CCNNNNNNNGG 5 cut(s) 88, 181, 432, 769, 861
AgsI TTSAA 2 cut(s) 103, 497
AleI CACNNNNGTG 1 cut(s) 137
AloI GAACNNNNNNTCC 2 cut(s) 657, 689
AluBI AGCT 3 cut(s) 659, 671, 751
AluI AGCT 3 cut(s) 659, 671, 751
Alw26I GTCTC 1 cut(s) 595
AlwI GGATC 2 cut(s) 309, 907
AlwNI CAGNNNCTG 1 cut(s) 92
AoxI GGCC 4 cut(s) 117, 434, 453, 852
ApeKI GCWGC 5 cut(s) 73, 295, 694, 751, 775
ApoI RAATTY 1 cut(s) 843
AspLEI GCGC 3 cut(s) 78, 565, 780
AspS9I GGNCC 2 cut(s) 117, 797
AvaII GGWCC 1 cut(s) 797
BalI TGGCCA 1 cut(s) 854
BanI GGYRCC 1 cut(s) 814
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 5 cut(s) 85, 282, 681, 738, 787
BccI CCATC 3 cut(s) 46, 504, 547
BclI TGATCA 1 cut(s) 388
BcoDI GTCTC 1 cut(s) 595
BfaI CTAG 2 cut(s) 44, 654
BfmI CTRYAG 1 cut(s) 296
BfoI RGCGCY 1 cut(s) 79
BisI GCNGC 6 cut(s) 74, 296, 695, 752, 776, 789
BlsI GCNGC 6 cut(s) 75, 297, 696, 753, 777, 790
Bme18I GGWCC 1 cut(s) 797
BmgT120I GGNCC 2 cut(s) 117, 797
BmiI GGNNCC 4 cut(s) 118, 161, 537, 816
BmsI GCATC 3 cut(s) 60, 183, 220
BpiI GAAGAC 1 cut(s) 68
Bsa29I ATCGAT 1 cut(s) 280
BsaJI CCNNGG 1 cut(s) 807
Bsc4I CCNNNNNNNGG 5 cut(s) 88, 181, 432, 769, 861
Bse1I ACTGG 1 cut(s) 667
BseCI ATCGAT 1 cut(s) 280
BseDI CCNNGG 1 cut(s) 807
BseGI GGATG 2 cut(s) 235, 515
BseLI CCNNNNNNNGG 5 cut(s) 88, 181, 432, 769, 861
BseMII CTCAG 1 cut(s) 99
BseNI ACTGG 1 cut(s) 667
BseRI GAGGAG 1 cut(s) 641
BseXI GCAGC 5 cut(s) 85, 282, 681, 738, 787
BseYI CCCAGC 1 cut(s) 697
BsgI GTGCAG 1 cut(s) 161
Bsh1285I CGRYCG 1 cut(s) 284
BshFI GGCC 4 cut(s) 119, 436, 455, 854
BshNI GGYRCC 1 cut(s) 814
BshVI ATCGAT 1 cut(s) 280
BsiEI CGRYCG 1 cut(s) 284
BslFI GGGAC 1 cut(s) 816
BslI CCNNNNNNNGG 5 cut(s) 88, 181, 432, 769, 861
BsmAI GTCTC 1 cut(s) 595
BsmBI CGTCTC 1 cut(s) 595
BsmFI GGGAC 1 cut(s) 816
BsmI GAATGC 2 cut(s) 297, 780
BsnI GGCC 4 cut(s) 119, 436, 455, 854
Bsp143I GATC 5 cut(s) 277, 281, 301, 388, 912
BspACI CCGC 3 cut(s) 423, 788, 862
BspANI GGCC 4 cut(s) 119, 436, 455, 854
BspCNI CTCAG 1 cut(s) 98
BspDI ATCGAT 1 cut(s) 280
BspLI GGNNCC 4 cut(s) 118, 161, 537, 816
BspMAI CTGCAG 1 cut(s) 300
BspPI GGATC 2 cut(s) 309, 907
BspT107I GGYRCC 1 cut(s) 814
BsrI ACTGG 1 cut(s) 667
BssECI CCNNGG 1 cut(s) 807
BssMI GATC 5 cut(s) 277, 281, 301, 388, 912
BssT1I CCWWGG 1 cut(s) 807
Bst4CI ACNGT 3 cut(s) 171, 589, 769
BstC8I GCNNGC 2 cut(s) 144, 333
BstDEI CTNAG 1 cut(s) 85
BstENI CCTNNNNNAGG 1 cut(s) 430
BstF5I GGATG 2 cut(s) 235, 515
BstH2I RGCGCY 1 cut(s) 79
BstHHI GCGC 3 cut(s) 78, 565, 780
BstKTI GATC 5 cut(s) 280, 284, 304, 391, 915
BstMAI GTCTC 1 cut(s) 595
BstMBI GATC 5 cut(s) 277, 281, 301, 388, 912
BstMCI CGRYCG 1 cut(s) 284
BstMWI GCNNNNNNNGC 2 cut(s) 152, 422
BstNSI RCATGY 2 cut(s) 274, 335
BstSFI CTRYAG 1 cut(s) 296
BstV1I GCAGC 5 cut(s) 85, 282, 681, 738, 787
BstV2I GAAGAC 1 cut(s) 68
Bsu15I ATCGAT 1 cut(s) 280
BsuRI GGCC 4 cut(s) 119, 436, 455, 854
BsuTUI ATCGAT 1 cut(s) 280
BtgZI GCGATG 1 cut(s) 525
BtsCI GGATG 2 cut(s) 235, 515
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 3 cut(s) 93, 316, 411
Cac8I GCNNGC 2 cut(s) 144, 333
CaiI CAGNNNCTG 1 cut(s) 92
CfoI GCGC 3 cut(s) 78, 565, 780
Cfr13I GGNCC 2 cut(s) 117, 797
ClaI ATCGAT 1 cut(s) 280
Csp6I GTAC 1 cut(s) 804
CviAII CATG 6 cut(s) 226, 271, 326, 332, 506, 801
CviQI GTAC 1 cut(s) 804
DdeI CTNAG 1 cut(s) 85
DpnI GATC 5 cut(s) 279, 283, 303, 390, 914
DpnII GATC 5 cut(s) 277, 281, 301, 388, 912
DraIII CACNNNGTG 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 852
Eco130I CCWWGG 1 cut(s) 807
Eco147I AGGCCT 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 797
EcoNI CCTNNNNNAGG 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 807
ErhI CCWWGG 1 cut(s) 807
Esp3I CGTCTC 1 cut(s) 595
FaeI CATG 6 cut(s) 229, 274, 329, 335, 509, 804
FaqI GGGAC 1 cut(s) 816
FatI CATG 6 cut(s) 225, 270, 325, 331, 505, 800
FauI CCCGC 1 cut(s) 855
FbaI TGATCA 1 cut(s) 388
Fnu4HI GCNGC 6 cut(s) 74, 296, 695, 752, 776, 789
FokI GGATG 2 cut(s) 242, 522
Fsp4HI GCNGC 6 cut(s) 74, 296, 695, 752, 776, 789
FspBI CTAG 2 cut(s) 44, 654
GlaI GCGC 3 cut(s) 77, 564, 779
GluI GCNGC 6 cut(s) 74, 296, 695, 752, 776, 789
GsaI CCCAGC 1 cut(s) 701
HaeII RGCGCY 1 cut(s) 79
HaeIII GGCC 4 cut(s) 119, 436, 455, 854
HhaI GCGC 3 cut(s) 78, 565, 780
Hin1II CATG 6 cut(s) 229, 274, 329, 335, 509, 804
Hin6I GCGC 3 cut(s) 76, 563, 778
HinP1I GCGC 3 cut(s) 76, 563, 778
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 5 cut(s) 27, 476, 602, 829, 867
HpaI GTTAAC 1 cut(s) 213
Hpy166II GTNNAC 2 cut(s) 213, 739
Hpy188I TCNGA 2 cut(s) 481, 912
Hpy188III TCNNGA 2 cut(s) 392, 430
Hpy8I GTNNAC 2 cut(s) 213, 739
HpyAV CCTTC 5 cut(s) 185, 243, 387, 436, 526
HpyCH4III ACNGT 3 cut(s) 171, 589, 769
HpyCH4V TGCA 8 cut(s) 73, 142, 225, 298, 331, 341, 383, 461
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 422
HpyF3I CTNAG 1 cut(s) 85
Hsp92II CATG 6 cut(s) 229, 274, 329, 335, 509, 804
HspAI GCGC 3 cut(s) 76, 563, 778
Ksp22I TGATCA 1 cut(s) 388
KspAI GTTAAC 1 cut(s) 213
Kzo9I GATC 5 cut(s) 277, 281, 301, 388, 912
LmnI GCTCC 1 cut(s) 772
Lsp1109I GCAGC 5 cut(s) 85, 282, 681, 738, 787
LweI GCATC 3 cut(s) 60, 183, 220
MaeI CTAG 2 cut(s) 44, 654
MaeIII GTNAC 2 cut(s) 603, 897
MalI GATC 5 cut(s) 279, 283, 303, 390, 914
MboI GATC 5 cut(s) 277, 281, 301, 388, 912
MboII GAAGA 4 cut(s) 33, 36, 73, 79
MfeI CAATTG 1 cut(s) 384
MlsI TGGCCA 1 cut(s) 854
MluCI AATT 4 cut(s) 36, 384, 570, 843
MluNI TGGCCA 1 cut(s) 854
MlyI GAGTC 2 cut(s) 611, 838
MnlI CCTC 8 cut(s) 4, 90, 426, 447, 619, 622, 642, 765
Mox20I TGGCCA 1 cut(s) 854
MscI TGGCCA 1 cut(s) 854
MseI TTAA 2 cut(s) 212, 321
MslI CAYNNNNRTG 3 cut(s) 137, 330, 714
Msp20I TGGCCA 1 cut(s) 854
MunI CAATTG 1 cut(s) 384
Mva1269I GAATGC 2 cut(s) 297, 780
MwoI GCNNNNNNNGC 2 cut(s) 152, 422
NdeII GATC 5 cut(s) 277, 281, 301, 388, 912
NlaIII CATG 6 cut(s) 229, 274, 329, 335, 509, 804
NlaIV GGNNCC 4 cut(s) 118, 161, 537, 816
NmuCI GTSAC 1 cut(s) 603
NspI RCATGY 2 cut(s) 274, 335
OliI CACNNNNGTG 1 cut(s) 137
PaeI GCATGC 1 cut(s) 335
PceI AGGCCT 1 cut(s) 436
PctI GAATGC 2 cut(s) 297, 780
PfeI GAWTC 3 cut(s) 27, 476, 867
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 6 cut(s) 75, 297, 696, 753, 777, 790
Ple19I CGATCG 1 cut(s) 284
PleI GAGTC 2 cut(s) 610, 837
PpsI GAGTC 2 cut(s) 610, 837
PspFI CCCAGC 1 cut(s) 697
PspN4I GGNNCC 4 cut(s) 118, 161, 537, 816
PspPI GGNCC 2 cut(s) 117, 797
PstI CTGCAG 1 cut(s) 300
PstNI CAGNNNCTG 1 cut(s) 92
PvuI CGATCG 1 cut(s) 284
RsaI GTAC 1 cut(s) 805
RsaNI GTAC 1 cut(s) 804
RseI CAYNNNNRTG 3 cut(s) 137, 330, 714
SaqAI TTAA 2 cut(s) 212, 321
SatI GCNGC 6 cut(s) 74, 296, 695, 752, 776, 789
Sau3AI GATC 5 cut(s) 277, 281, 301, 388, 912
Sau96I GGNCC 2 cut(s) 117, 797
SchI GAGTC 2 cut(s) 611, 838
SetI ASST 9 cut(s) 101, 137, 218, 494, 661, 673, 753, 809, 884
SfaNI GCATC 3 cut(s) 60, 183, 220
SfcI CTRYAG 1 cut(s) 296
SinI GGWCC 1 cut(s) 797
SmiMI CAYNNNNRTG 3 cut(s) 137, 330, 714
SphI GCATGC 1 cut(s) 335
Sse9I AATT 4 cut(s) 36, 384, 570, 843
SseBI AGGCCT 1 cut(s) 436
SsiI CCGC 3 cut(s) 423, 788, 862
SspMI CTAG 2 cut(s) 44, 654
StuI AGGCCT 1 cut(s) 436
StyI CCWWGG 1 cut(s) 807
TaaI ACNGT 3 cut(s) 171, 589, 769
TaqI TCGA 3 cut(s) 280, 429, 746
TasI AATT 4 cut(s) 36, 384, 570, 843
TauI GCSGC 1 cut(s) 791
TfiI GAWTC 3 cut(s) 27, 476, 867
Tru1I TTAA 2 cut(s) 212, 321
Tru9I TTAA 2 cut(s) 212, 321
TscAI CASTG 3 cut(s) 93, 316, 418
TseFI GTSAC 1 cut(s) 603
TseI GCWGC 5 cut(s) 73, 295, 694, 751, 775
Tsp45I GTSAC 1 cut(s) 603
TspRI CASTG 3 cut(s) 93, 316, 418
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 797
XagI CCTNNNNNAGG 1 cut(s) 430
XapI RAATTY 1 cut(s) 843
XceI RCATGY 2 cut(s) 274, 335
XspI CTAG 2 cut(s) 44, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.