pycom16g13250

Dihydrolipoyl dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0029475
Physical Location & Seq
Forward (+)
9424712 .. 9426547
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 840 bp
ATGCCTGGTTTTGATCCTGAGATTGGAAAGTTAGCCCAAAGGGTTCTAATTAATCCACGGAAGATTGATTATCAAACTGGGGTATTCGCATCCAAGATCACACCAGCCAAGGATGGGAAACCAGTCACCATTGAACTAATTGATGCAAGGACCAAGGAACCAAAAGAAACCCTTGAGGTAGATGCAGCACTAATTGCAACAGGAAGAGCGCCATTCACACAAGGTCTTGGATTGGAGAATGTCGATGTAGCAACACAACGTGGCTTTATTCCTGTTGATGAGCGCATGCGAGTAATAGATGCAAATGGAAAATTGGTTCCTCACCTATTTTGCATTGGTGATGCTAATGGCAAGATGATGCTTGCTCATGCAGCCAGTGCACAAGGAATTTCAGTGGTTGAACAAGTCACAGGAAGAGACCACGTGCTCAATCATTTGAGCATTCCTGCAGCATGTTTCACTCATCCAGAAATCAGTATGGTTGGATTGACCGAGCCTCAAGCAAGGGAGAAAGCTGAAAAGGAGGGATTTGAAGTAAGTATCGCCAAGACCAGTTTCAAGGCCAACACAAAGGCCTTAGCAGAAAATGAAGGAGAGGGACTCGCTAAGTTGATATACAGACCAGATAATGGAGAGATTCTTGGAGTTCATATATTTGGGCTACATGCCGCAGACCTTATTCATGAGGCATCAAATGCGATAGCACTGGGGACACGTATTCAAGATATCAAATTTGCAGTCCATGCACACCCTACTTTGTCCGAAGTCCTAGACGAACTTTTCAAGTCAGCAAAGGTAGCTCACGTTTCTAGCCCAGTAAGTGAACCAGTTGCAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004148 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006082 GO:0006084 GO:0006085 GO:0006086 GO:0006090 GO:0006139 GO:0006163 GO:0006164 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0009987 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019752 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032787 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035383 GO:0035384 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046390 GO:0046483 GO:0046685 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0055114 GO:0070013 GO:0071616 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00260 ko00280 ko00620 ko00630 ko00640 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00260 map00280 map00620 map00630 map00640 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
Pyr_redox_2 Pyr_redox_dim

Protein Analysis

280

Amino Acids

29.94

Weight (kDa)

5.89

Isoelectric Point (pI)

22.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 1 - 129 3.3e-15 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_dim PF02852 148 - 259 4.7e-31 Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029475)

Species Orthologous Gene IDs
pyrus_communis pycom16g13250
rosa_multiflora Rmu_sc0003567.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 629
AciI CCGC 1 cut(s) 669
AclWI GGATC 1 cut(s) 8
AcsI RAATTY 2 cut(s) 387, 731
AcvI CACGTG 1 cut(s) 424
AdeI CACNNNGTG 1 cut(s) 260
AfiI CCNNNNNNNGG 3 cut(s) 23, 114, 629
AflIII ACRYGT 1 cut(s) 713
AgsI TTSAA 6 cut(s) 134, 401, 533, 559, 722, 784
AjnI CCWGG 1 cut(s) 4
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
AloI GAACNNNNNNTCC 2 cut(s) 300, 332
AluBI AGCT 2 cut(s) 515, 800
AluI AGCT 2 cut(s) 515, 800
Alw21I GWGCWC 2 cut(s) 382, 429
Alw26I GTCTC 1 cut(s) 411
Alw44I GTGCAC 1 cut(s) 378
AlwI GGATC 1 cut(s) 8
AoxI GGCC 2 cut(s) 561, 573
ApaLI GTGCAC 1 cut(s) 378
ApeKI GCWGC 3 cut(s) 185, 371, 449
ApoI RAATTY 2 cut(s) 387, 731
ArsI GACNNNNNNTTYG 2 cut(s) 723, 755
AseI ATTAAT 1 cut(s) 51
AspLEI GCGC 2 cut(s) 211, 285
AspS9I GGNCC 1 cut(s) 150
AsuHPI GGTGA 3 cut(s) 118, 314, 350
AvaII GGWCC 1 cut(s) 150
BaeGI GKGCMC 1 cut(s) 382
BbrPI CACGTG 1 cut(s) 424
Bbv12I GWGCWC 2 cut(s) 382, 429
BbvI GCAGC 3 cut(s) 197, 383, 461
BccI CCATC 1 cut(s) 107
BciT130I CCWGG 1 cut(s) 6
BcoDI GTCTC 1 cut(s) 411
BfaI CTAG 2 cut(s) 770, 810
BfmI CTRYAG 1 cut(s) 447
BfoI RGCGCY 1 cut(s) 212
BisI GCNGC 4 cut(s) 186, 372, 450, 669
BlsI GCNGC 4 cut(s) 187, 373, 451, 670
Bme1390I CCNGG 1 cut(s) 6
Bme18I GGWCC 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 150
BmiI GGNNCC 2 cut(s) 159, 318
BmrFI CCNGG 1 cut(s) 6
BmrI ACTGGG 3 cut(s) 87, 716, 809
BmsI GCATC 7 cut(s) 98, 133, 172, 289, 331, 348, 698
BmuI ACTGGG 3 cut(s) 87, 716, 809
BplI GAGNNNNNCTC 2 cut(s) 585, 617
Bpu10I CCTNAGC 1 cut(s) 577
BpuEI CTTGAG 2 cut(s) 194, 483
BsaAI YACGTR 2 cut(s) 424, 716
BsaI GGTCTC 1 cut(s) 411
BsaJI CCNNGG 3 cut(s) 56, 108, 153
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 114, 629
Bse1I ACTGG 7 cut(s) 82, 122, 375, 552, 711, 815, 827
BseBI CCWGG 1 cut(s) 6
BseDI CCNNGG 3 cut(s) 56, 108, 153
BseGI GGATG 3 cut(s) 89, 118, 463
BseLI CCNNNNNNNGG 3 cut(s) 23, 114, 629
BseMII CTCAG 1 cut(s) 9
BseNI ACTGG 7 cut(s) 82, 122, 375, 552, 711, 815, 827
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 3 cut(s) 197, 383, 461
BshFI GGCC 2 cut(s) 563, 575
BsiHKAI GWGCWC 2 cut(s) 382, 429
BslFI GGGAC 2 cut(s) 612, 724
BslI CCNNNNNNNGG 3 cut(s) 23, 114, 629
BsmAI GTCTC 1 cut(s) 411
BsmFI GGGAC 2 cut(s) 612, 724
BsmI GAATGC 1 cut(s) 441
BsnI GGCC 2 cut(s) 563, 575
Bso31I GGTCTC 1 cut(s) 411
Bsp1286I GDGCHC 2 cut(s) 382, 429
Bsp143I GATC 2 cut(s) 13, 96
BspACI CCGC 1 cut(s) 669
BspANI GGCC 2 cut(s) 563, 575
BspCNI CTCAG 1 cut(s) 10
BspHI TCATGA 1 cut(s) 682
BspLI GGNNCC 2 cut(s) 159, 318
BspMAI CTGCAG 1 cut(s) 451
BspPI GGATC 1 cut(s) 8
BspQI GCTCTTC 1 cut(s) 199
BspTNI GGTCTC 1 cut(s) 411
BsrI ACTGG 7 cut(s) 82, 122, 375, 552, 711, 815, 827
BssECI CCNNGG 3 cut(s) 56, 108, 153
BssMI GATC 2 cut(s) 13, 96
BssT1I CCWWGG 2 cut(s) 108, 153
Bst2UI CCWGG 1 cut(s) 6
Bst6I CTCTTC 2 cut(s) 199, 409
BstAPI GCANNNNNTGC 4 cut(s) 194, 377, 695, 743
BstBAI YACGTR 2 cut(s) 424, 716
BstC8I GCNNGC 2 cut(s) 287, 363
BstDEI CTNAG 3 cut(s) 18, 577, 606
BstDSI CCRYGG 1 cut(s) 56
BstF5I GGATG 3 cut(s) 89, 118, 463
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 2 cut(s) 211, 285
BstKTI GATC 2 cut(s) 16, 99
BstMAI GTCTC 1 cut(s) 411
BstMBI GATC 2 cut(s) 13, 96
BstMWI GCNNNNNNNGC 6 cut(s) 194, 371, 377, 695, 743, 797
BstNI CCWGG 1 cut(s) 6
BstNSI RCATGY 3 cut(s) 289, 456, 668
BstSCI CCNGG 1 cut(s) 4
BstSFI CTRYAG 1 cut(s) 447
BstSLI GKGCMC 1 cut(s) 382
BstV1I GCAGC 3 cut(s) 197, 383, 461
BsuRI GGCC 2 cut(s) 563, 575
BtgI CCRYGG 1 cut(s) 56
BtsCI GGATG 3 cut(s) 89, 118, 463
BtsIMutI CAGTG 3 cut(s) 382, 399, 704
Cac8I GCNNGC 2 cut(s) 287, 363
CciI TCATGA 1 cut(s) 682
CfoI GCGC 2 cut(s) 211, 285
Cfr13I GGNCC 1 cut(s) 150
CviAII CATG 6 cut(s) 286, 368, 453, 665, 683, 743
DdeI CTNAG 3 cut(s) 18, 577, 606
DpnI GATC 2 cut(s) 15, 98
DpnII GATC 2 cut(s) 13, 96
DraIII CACNNNGTG 1 cut(s) 260
Eam1104I CTCTTC 2 cut(s) 199, 409
EarI CTCTTC 2 cut(s) 199, 409
Eco130I CCWWGG 2 cut(s) 108, 153
Eco147I AGGCCT 1 cut(s) 575
Eco31I GGTCTC 1 cut(s) 411
Eco32I GATATC 1 cut(s) 727
Eco47I GGWCC 1 cut(s) 150
Eco72I CACGTG 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 4
EcoRV GATATC 1 cut(s) 727
EcoT14I CCWWGG 2 cut(s) 108, 153
ErhI CCWWGG 2 cut(s) 108, 153
FaeI CATG 6 cut(s) 289, 371, 456, 668, 686, 746
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FaqI GGGAC 2 cut(s) 612, 724
FatI CATG 6 cut(s) 285, 367, 452, 664, 682, 742
Fnu4HI GCNGC 4 cut(s) 186, 372, 450, 669
FokI GGATG 3 cut(s) 76, 125, 450
Fsp4HI GCNGC 4 cut(s) 186, 372, 450, 669
FspBI CTAG 2 cut(s) 770, 810
GlaI GCGC 2 cut(s) 210, 284
GluI GCNGC 4 cut(s) 186, 372, 450, 669
HaeII RGCGCY 1 cut(s) 212
HaeIII GGCC 2 cut(s) 563, 575
HhaI GCGC 2 cut(s) 211, 285
Hin1II CATG 6 cut(s) 289, 371, 456, 668, 686, 746
Hin6I GCGC 2 cut(s) 209, 283
HinP1I GCGC 2 cut(s) 209, 283
HinfI GANTC 2 cut(s) 600, 637
HphI GGTGA 3 cut(s) 118, 314, 350
Hpy166II GTNNAC 2 cut(s) 380, 824
Hpy188I TCNGA 1 cut(s) 763
Hpy188III TCNNGA 4 cut(s) 17, 467, 683, 722
Hpy8I GTNNAC 2 cut(s) 380, 824
HpyAV CCTTC 1 cut(s) 584
HpyCH4IV ACGT 4 cut(s) 259, 423, 715, 804
HpyF10VI GCNNNNNNNGC 6 cut(s) 194, 371, 377, 695, 743, 797
HpyF3I CTNAG 3 cut(s) 18, 577, 606
HpySE526I ACGT 4 cut(s) 259, 423, 715, 804
Hsp92II CATG 6 cut(s) 289, 371, 456, 668, 686, 746
HspAI GCGC 2 cut(s) 209, 283
Kzo9I GATC 2 cut(s) 13, 96
LguI GCTCTTC 1 cut(s) 199
Lsp1109I GCAGC 3 cut(s) 197, 383, 461
LweI GCATC 7 cut(s) 98, 133, 172, 289, 331, 348, 698
MaeI CTAG 2 cut(s) 770, 810
MaeII ACGT 4 cut(s) 259, 423, 715, 804
MaeIII GTNAC 2 cut(s) 124, 406
MalI GATC 2 cut(s) 15, 98
MboI GATC 2 cut(s) 13, 96
MboII GAAGA 3 cut(s) 73, 216, 426
MhlI GDGCHC 2 cut(s) 382, 429
MluCI AATT 6 cut(s) 48, 138, 192, 311, 387, 731
MlyI GAGTC 1 cut(s) 594
MmeI TCCRAC 1 cut(s) 463
MnlI CCTC 6 cut(s) 169, 330, 507, 517, 589, 679
MseI TTAA 1 cut(s) 51
MspR9I CCNGG 1 cut(s) 6
Mva1269I GAATGC 1 cut(s) 441
MvaI CCWGG 1 cut(s) 6
MwoI GCNNNNNNNGC 6 cut(s) 194, 371, 377, 695, 743, 797
NdeII GATC 2 cut(s) 13, 96
NlaIII CATG 6 cut(s) 289, 371, 456, 668, 686, 746
NlaIV GGNNCC 2 cut(s) 159, 318
NmuCI GTSAC 2 cut(s) 124, 406
NspI RCATGY 3 cut(s) 289, 456, 668
PaeI GCATGC 1 cut(s) 289
PagI TCATGA 1 cut(s) 682
PceI AGGCCT 1 cut(s) 575
PciSI GCTCTTC 1 cut(s) 199
PctI GAATGC 1 cut(s) 441
PfeI GAWTC 1 cut(s) 637
PflMI CCANNNNNTGG 1 cut(s) 629
PkrI GCNGC 4 cut(s) 187, 373, 451, 670
PleI GAGTC 1 cut(s) 594
PmaCI CACGTG 1 cut(s) 424
PmlI CACGTG 1 cut(s) 424
PpsI GAGTC 1 cut(s) 594
Ppu21I YACGTR 2 cut(s) 424, 716
PshBI ATTAAT 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 4
PspCI CACGTG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 4
PspN4I GGNNCC 2 cut(s) 159, 318
PspPI GGNCC 1 cut(s) 150
PstI CTGCAG 1 cut(s) 451
SapI GCTCTTC 1 cut(s) 199
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 4 cut(s) 186, 372, 450, 669
Sau3AI GATC 2 cut(s) 13, 96
Sau96I GGNCC 1 cut(s) 150
SchI GAGTC 1 cut(s) 594
ScrFI CCNGG 1 cut(s) 6
SduI GDGCHC 2 cut(s) 382, 429
SfaNI GCATC 7 cut(s) 98, 133, 172, 289, 331, 348, 698
SfcI CTRYAG 1 cut(s) 447
SinI GGWCC 1 cut(s) 150
SmlI CTYRAG 2 cut(s) 173, 498
SmoI CTYRAG 2 cut(s) 173, 498
SphI GCATGC 1 cut(s) 289
Sse9I AATT 6 cut(s) 48, 138, 192, 311, 387, 731
SseBI AGGCCT 1 cut(s) 575
SsiI CCGC 1 cut(s) 669
SspMI CTAG 2 cut(s) 770, 810
StuI AGGCCT 1 cut(s) 575
StyD4I CCNGG 1 cut(s) 4
StyI CCWWGG 2 cut(s) 108, 153
TaiI ACGT 4 cut(s) 262, 426, 718, 807
TaqI TCGA 1 cut(s) 243
TaqII GACCGA 1 cut(s) 506
TasI AATT 6 cut(s) 48, 138, 192, 311, 387, 731
TauI GCSGC 1 cut(s) 671
TfiI GAWTC 1 cut(s) 637
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 3 cut(s) 382, 399, 711
TseFI GTSAC 2 cut(s) 124, 406
TseI GCWGC 3 cut(s) 185, 371, 449
Tsp45I GTSAC 2 cut(s) 124, 406
TspDTI ATGAA 3 cut(s) 603, 638, 671
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 3 cut(s) 382, 399, 711
Van91I CCANNNNNTGG 1 cut(s) 629
VneI GTGCAC 1 cut(s) 378
VpaK11BI GGWCC 1 cut(s) 150
VspI ATTAAT 1 cut(s) 51
XapI RAATTY 2 cut(s) 387, 731
XceI RCATGY 3 cut(s) 289, 456, 668
XspI CTAG 2 cut(s) 770, 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.