pycom16g16810

Catalyzes the formation of formate and 2-keto-4- methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5- methylthiopentene (DHK-MTPene)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0024706
Physical Location & Seq
Forward (+)
12823688 .. 12824534
847 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 201 bp
ATGATTGTTTTGCCTGCTGGAATTTATCACCGATTTACATTGGACACAAACTACTACATAAAGGCAATGCGTCTCTTTATTGGCGATCCAGTTTGGACTCCCTATAATCGTCCTCATGATCATCTTCCCGCAAGGGAGGAGTATCTTAAAGCTTTTGTGCGTAAGGAAACTGGTGACCATGCAGTTAATGCTGCGGCATAA

Protein Analysis

67

Amino Acids

7.67

Weight (kDa)

8.11

Isoelectric Point (pI)

21.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARD PF03079 1 - 34 4.8e-12 ARD/ARD' family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024706)

Species Orthologous Gene IDs
pyrus_communis pycom16g16810
rosa_multiflora Rmu_sc0003953.1_g000032 Rmu_sc0005661.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 129, 194
AclWI GGATC 1 cut(s) 80
AcsI RAATTY 1 cut(s) 21
AluBI AGCT 1 cut(s) 152
AluI AGCT 1 cut(s) 152
Alw26I GTCTC 1 cut(s) 77
AlwI GGATC 1 cut(s) 80
ApeKI GCWGC 1 cut(s) 191
ApoI RAATTY 1 cut(s) 21
AsuHPI GGTGA 2 cut(s) 20, 185
BbvI GCAGC 1 cut(s) 178
BclI TGATCA 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 77
BisI GCNGC 2 cut(s) 192, 195
BlsI GCNGC 2 cut(s) 193, 196
Bse1I ACTGG 2 cut(s) 89, 175
Bse3DI GCAATG 1 cut(s) 72
BseMI GCAATG 1 cut(s) 72
BseNI ACTGG 2 cut(s) 89, 175
BseRI GAGGAG 1 cut(s) 152
BseXI GCAGC 1 cut(s) 178
BsmAI GTCTC 1 cut(s) 77
BsmBI CGTCTC 1 cut(s) 77
Bsp143I GATC 2 cut(s) 85, 118
BspACI CCGC 2 cut(s) 129, 194
BspHI TCATGA 1 cut(s) 115
BspPI GGATC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 72
BsrI ACTGG 2 cut(s) 89, 175
BssMI GATC 2 cut(s) 85, 118
BstAPI GCANNNNNTGC 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 15
BstEII GGTNACC 1 cut(s) 173
BstKTI GATC 2 cut(s) 88, 121
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 2 cut(s) 85, 118
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstPI GGTNACC 1 cut(s) 173
BstV1I GCAGC 1 cut(s) 178
Cac8I GCNNGC 1 cut(s) 15
CciI TCATGA 1 cut(s) 115
CseI GACGC 1 cut(s) 59
CviAII CATG 2 cut(s) 116, 179
CviJI RGCY 1 cut(s) 152
CviKI_1 RGCY 1 cut(s) 152
DpnI GATC 2 cut(s) 87, 120
DpnII GATC 2 cut(s) 85, 118
Eco91I GGTNACC 1 cut(s) 173
EcoO65I GGTNACC 1 cut(s) 173
Esp3I CGTCTC 1 cut(s) 77
FaeI CATG 2 cut(s) 119, 182
FaiI YATR 5 cut(s) 59, 105, 117, 180, 199
FatI CATG 2 cut(s) 115, 178
FauI CCCGC 1 cut(s) 136
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 2 cut(s) 192, 195
Fsp4HI GCNGC 2 cut(s) 192, 195
GluI GCNGC 2 cut(s) 192, 195
HgaI GACGC 1 cut(s) 59
Hin1II CATG 2 cut(s) 119, 182
HindIII AAGCTT 1 cut(s) 150
HinfI GANTC 1 cut(s) 97
HphI GGTGA 2 cut(s) 20, 185
Hpy188III TCNNGA 1 cut(s) 116
HpyCH4V TGCA 1 cut(s) 182
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
Hsp92II CATG 2 cut(s) 119, 182
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 2 cut(s) 85, 118
LpnPI CCDG 4 cut(s) 3, 27, 102, 156
Lsp1109I GCAGC 1 cut(s) 178
MaeIII GTNAC 1 cut(s) 173
MalI GATC 2 cut(s) 87, 120
MboI GATC 2 cut(s) 85, 118
MboII GAAGA 1 cut(s) 116
MluCI AATT 1 cut(s) 21
MlyI GAGTC 1 cut(s) 91
MnlI CCTC 2 cut(s) 123, 130
MseI TTAA 2 cut(s) 147, 186
MwoI GCNNNNNNNGC 1 cut(s) 188
NdeII GATC 2 cut(s) 85, 118
NlaIII CATG 2 cut(s) 119, 182
NmuCI GTSAC 1 cut(s) 173
PagI TCATGA 1 cut(s) 115
PkrI GCNGC 2 cut(s) 193, 196
PleI GAGTC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 91
PspEI GGTNACC 1 cut(s) 173
SaqAI TTAA 2 cut(s) 147, 186
SatI GCNGC 2 cut(s) 192, 195
Sau3AI GATC 2 cut(s) 85, 118
SchI GAGTC 1 cut(s) 91
SetI ASST 1 cut(s) 154
SgeI CNNG 8 cut(s) 26, 30, 101, 128, 140, 144, 183, 191
Sse9I AATT 1 cut(s) 21
SsiI CCGC 2 cut(s) 129, 194
TasI AATT 1 cut(s) 21
TauI GCSGC 1 cut(s) 197
Tru1I TTAA 2 cut(s) 147, 186
Tru9I TTAA 2 cut(s) 147, 186
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 173
XapI RAATTY 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.