pycom17g01490

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0029484
Physical Location & Seq
Forward (+)
1004365 .. 1005492
1128 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGTTCTTGGGTGAACTGGAAGAAGTTTTAGAGGCAACTCAGCCTGCAGAGTTTCAGCGCTGTATGGTACCGCTGTTTCGCCAGATTGGTCGTTGCTTGAGCAGTTCACATTTTCAGGTGGCAGAGAGAGCACTGTTCTTATGGAATAATGATCACATTGCGAACCTAATCAAACAGAATCGCCATGTCTTACTGCCAATCATATTCCCTTCATTGGAGAAAAATGCAAGAAACCACTGGAACCAGGCAGTACAGAGCCTGACACTAAATGTCCGTAAGATTTTCTCCGATATTGACCCTGAGCTTTTTGAGGAATGTTTGCTCAACTTCCAGGAAGATGAAGCACAAGCGAGTGTAAAAAGTTTGAAACGTCAAAACACCTGGAAACGTTTGGAAGAGATTGCGGCAAAGAATGCTACAAGCAATGAAGCCGTGCTTGTTTCCCCCAGAGGAGCCTCCGGCAAAACTTCTGGCTAG

Protein Analysis

159

Amino Acids

18.02

Weight (kDa)

6.43

Isoelectric Point (pI)

50.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 1 - 135 8.4e-54 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0029484)

Species Orthologous Gene IDs
pyrus_communis pycom17g01490
rosa_multiflora Rmu_co8270105.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 67
AccB1I GGYRCC 1 cut(s) 67
AciI CCGC 2 cut(s) 71, 404
AclI AACGTT 1 cut(s) 388
AfaI GTAC 2 cut(s) 69, 252
AfeI AGCGCT 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 3 cut(s) 243, 330, 380
AluBI AGCT 1 cut(s) 304
AluI AGCT 1 cut(s) 304
Alw21I GWGCWC 1 cut(s) 133
AlwNI CAGNNNCTG 1 cut(s) 259
Aor51HI AGCGCT 1 cut(s) 59
Asp718I GGTACC 1 cut(s) 67
AspLEI GCGC 1 cut(s) 60
AsuHPI GGTGA 1 cut(s) 23
BaeI ACNNNNGTAYC 2 cut(s) 59, 92
BanI GGYRCC 1 cut(s) 67
Bbv12I GWGCWC 1 cut(s) 133
BceAI ACGGC 1 cut(s) 416
BciT130I CCWGG 3 cut(s) 245, 332, 382
BclI TGATCA 1 cut(s) 151
BfaI CTAG 1 cut(s) 475
BfmI CTRYAG 1 cut(s) 45
BfoI RGCGCY 1 cut(s) 61
BisI GCNGC 1 cut(s) 405
BlsI GCNGC 1 cut(s) 406
Bme1390I CCNGG 3 cut(s) 245, 332, 382
BmiI GGNNCC 3 cut(s) 69, 242, 454
BmrFI CCNGG 3 cut(s) 245, 332, 382
Bpu10I CCTNAGC 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 118
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse1I ACTGG 2 cut(s) 21, 242
Bse3DI GCAATG 2 cut(s) 156, 430
BseBI CCWGG 3 cut(s) 245, 332, 382
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMI GCAATG 2 cut(s) 156, 430
BseMII CTCAG 2 cut(s) 53, 291
BseNI ACTGG 2 cut(s) 21, 242
BseRI GAGGAG 1 cut(s) 465
BshNI GGYRCC 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 133
BsiSI CCGG 1 cut(s) 459
BslI CCNNNNNNNGG 1 cut(s) 214
BsmI GAATGC 1 cut(s) 418
Bsp1286I GDGCHC 1 cut(s) 133
Bsp143I GATC 1 cut(s) 151
BspACI CCGC 2 cut(s) 71, 404
BspCNI CTCAG 2 cut(s) 52, 292
BspLI GGNNCC 3 cut(s) 69, 242, 454
BspMAI CTGCAG 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 67
BsrDI GCAATG 2 cut(s) 156, 430
BsrI ACTGG 2 cut(s) 21, 242
BssMI GATC 1 cut(s) 151
Bst2UI CCWGG 3 cut(s) 245, 332, 382
Bst4CI ACNGT 1 cut(s) 135
Bst6I CTCTTC 1 cut(s) 390
BstAPI GCANNNNNTGC 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 2 cut(s) 39, 300
BstH2I RGCGCY 1 cut(s) 61
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 1 cut(s) 154
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 2 cut(s) 128, 413
BstNI CCWGG 3 cut(s) 245, 332, 382
BstSCI CCNGG 3 cut(s) 243, 330, 380
BstSFI CTRYAG 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 131, 235
Cac8I GCNNGC 1 cut(s) 45
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 1 cut(s) 60
Csp6I GTAC 2 cut(s) 68, 251
CviAII CATG 1 cut(s) 185
CviJI RGCY 6 cut(s) 43, 258, 304, 431, 455, 474
CviKI_1 RGCY 6 cut(s) 43, 258, 304, 431, 455, 474
CviQI GTAC 2 cut(s) 68, 251
DdeI CTNAG 2 cut(s) 39, 300
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
Eco47III AGCGCT 1 cut(s) 59
EcoRII CCWGG 3 cut(s) 243, 330, 380
FaeI CATG 1 cut(s) 188
FaiI YATR 4 cut(s) 65, 142, 186, 203
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FatI CATG 1 cut(s) 184
FbaI TGATCA 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 405
Fsp4HI GCNGC 1 cut(s) 405
FspBI CTAG 1 cut(s) 475
GlaI GCGC 1 cut(s) 59
GluI GCNGC 1 cut(s) 405
HaeII RGCGCY 1 cut(s) 61
HapII CCGG 1 cut(s) 459
HhaI GCGC 1 cut(s) 60
Hin1II CATG 1 cut(s) 188
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HinfI GANTC 1 cut(s) 178
HpaII CCGG 1 cut(s) 459
HphI GGTGA 1 cut(s) 23
Hpy166II GTNNAC 2 cut(s) 14, 107
Hpy188I TCNGA 1 cut(s) 289
Hpy8I GTNNAC 2 cut(s) 14, 107
HpyAV CCTTC 1 cut(s) 219
HpyCH4III ACNGT 1 cut(s) 135
HpyCH4IV ACGT 2 cut(s) 370, 388
HpyCH4V TGCA 2 cut(s) 47, 227
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 413
HpyF3I CTNAG 2 cut(s) 39, 300
HpySE526I ACGT 2 cut(s) 370, 388
Hsp92II CATG 1 cut(s) 188
HspAI GCGC 1 cut(s) 58
KpnI GGTACC 1 cut(s) 71
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 1 cut(s) 151
LmnI GCTCC 1 cut(s) 452
MaeI CTAG 1 cut(s) 475
MaeII ACGT 2 cut(s) 370, 388
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 3 cut(s) 32, 347, 407
MhlI GDGCHC 1 cut(s) 133
MnlI CCTC 4 cut(s) 25, 304, 443, 466
MspA1I CMGCKG 1 cut(s) 73
MspI CCGG 1 cut(s) 459
MspR9I CCNGG 3 cut(s) 245, 332, 382
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 3 cut(s) 245, 332, 382
MwoI GCNNNNNNNGC 2 cut(s) 128, 413
NdeII GATC 1 cut(s) 151
NlaIII CATG 1 cut(s) 188
NlaIV GGNNCC 3 cut(s) 69, 242, 454
PctI GAATGC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 178
PfoI TCCNGGA 1 cut(s) 330
PkrI GCNGC 1 cut(s) 406
Psp1406I AACGTT 1 cut(s) 388
Psp6I CCWGG 3 cut(s) 243, 330, 380
PspGI CCWGG 3 cut(s) 243, 330, 380
PspN4I GGNNCC 3 cut(s) 69, 242, 454
PstI CTGCAG 1 cut(s) 49
PstNI CAGNNNCTG 1 cut(s) 259
RsaI GTAC 2 cut(s) 69, 252
RsaNI GTAC 2 cut(s) 68, 251
SatI GCNGC 1 cut(s) 405
Sau3AI GATC 1 cut(s) 151
ScrFI CCNGG 3 cut(s) 245, 332, 382
SduI GDGCHC 1 cut(s) 133
SetI ASST 6 cut(s) 120, 168, 306, 373, 383, 391
SfcI CTRYAG 1 cut(s) 45
SmlI CTYRAG 1 cut(s) 97
SmoI CTYRAG 1 cut(s) 97
SsiI CCGC 2 cut(s) 71, 404
SspMI CTAG 1 cut(s) 475
StyD4I CCNGG 3 cut(s) 243, 330, 380
TaaI ACNGT 1 cut(s) 135
TaiI ACGT 2 cut(s) 373, 391
TatI WGTACW 1 cut(s) 250
TauI GCSGC 1 cut(s) 407
TfiI GAWTC 1 cut(s) 178
TscAI CASTG 2 cut(s) 138, 242
TspDTI ATGAA 3 cut(s) 201, 354, 441
TspGWI ACGGA 1 cut(s) 263
TspRI CASTG 2 cut(s) 138, 242
XspI CTAG 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.