pycom17g13680

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0028781
Physical Location & Seq
Forward (+)
11249495 .. 11249963
469 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 429 bp
ATGCACGATCTTCCGCCGGTGGAGGTAGGCGAGGCCGCTGAGGATTTGCCTGGCCAAATCGGAGAGGGCCCTCTCGTTCCCGATGTGCTTGCCCTCCAGCGACCCGCCGTCCATGTCGTGGCACTTGACGACGTTGGGGTTATCGACGTCACGCAGGATTTCGATCTCGCGGCAGATCTGGCGGAGGGTTTCGATCTCGTGGTTGCCGTAAATCACCTTGAGCGCGTAAAGATGGCCCGTGGGGCGGTGGATGACCTTGTAGACGGTGCCTCCGGCTCCGCTCCCGATGCGGTTGGAGCGCTCGAGCTCGGAGAAGTTGATGAGCTGCTGGGCCGCGCTGTGGGCCGAGGAGGACTGGGCCGAGCTGGGGCCGGGCGGAGGGAGAGGGAGCGGCACAGCAAGCGATGTGTCCCGCTGAGGGAGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

143

Amino Acids

14.77

Weight (kDa)

4.54

Isoelectric Point (pI)

31.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028781)

Species Orthologous Gene IDs
malus_domestica MD17G1143700.v1.1
pyrus_communis pycom17g13680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccB1I GGYRCC 1 cut(s) 266
AccB7I CCANNNNNTGG 1 cut(s) 118
AccBSI CCGCTC 2 cut(s) 281, 391
AccI GTMKAC 1 cut(s) 261
AccII CGCG 3 cut(s) 170, 225, 336
AcoI YGGCCR 1 cut(s) 52
AcyI GRCGYC 1 cut(s) 147
AfeI AGCGCT 1 cut(s) 300
AfiI CCNNNNNNNGG 6 cut(s) 118, 244, 340, 367, 378, 418
AhdI GACNNNNNGTC 1 cut(s) 107
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 3 cut(s) 307, 325, 365
AluI AGCT 3 cut(s) 307, 325, 365
Alw21I GWGCWC 1 cut(s) 309
Ama87I CYCGRG 1 cut(s) 302
Aor51HI AGCGCT 1 cut(s) 300
AoxI GGCC 8 cut(s) 33, 52, 67, 234, 331, 343, 358, 369
ApaI GGGCCC 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 325
AspLEI GCGC 3 cut(s) 225, 301, 338
AspS9I GGNCC 7 cut(s) 67, 68, 235, 331, 343, 358, 369
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 1 cut(s) 206
AvaI CYCGRG 1 cut(s) 302
BaeGI GKGCMC 1 cut(s) 71
BalI TGGCCA 1 cut(s) 54
BanI GGYRCC 1 cut(s) 266
BanII GRGCYC 2 cut(s) 71, 309
BauI CACGAG 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 309
BbvCI CCTCAGC 2 cut(s) 39, 416
BbvI GCAGC 1 cut(s) 312
BccI CCATC 1 cut(s) 226
BceAI ACGGC 2 cut(s) 92, 191
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 1 cut(s) 51
BcnI CCSGG 1 cut(s) 373
BfoI RGCGCY 1 cut(s) 302
BglI GCCNNNNNGGC 1 cut(s) 242
BglII AGATCT 1 cut(s) 175
BisI GCNGC 5 cut(s) 36, 171, 326, 334, 392
BlsI GCNGC 5 cut(s) 37, 172, 327, 335, 393
Bme1390I CCNGG 2 cut(s) 51, 373
BmeRI GACNNNNNGTC 1 cut(s) 107
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 7 cut(s) 67, 68, 235, 331, 343, 358, 369
BmiI GGNNCC 4 cut(s) 69, 268, 277, 370
BmrFI CCNGG 2 cut(s) 51, 373
BmrI ACTGGG 1 cut(s) 365
BmsI GCATC 1 cut(s) 277
BmuI ACTGGG 1 cut(s) 365
BpmI CTGGAG 1 cut(s) 80
Bpu10I CCTNAGC 2 cut(s) 39, 416
BpuEI CTTGAG 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 373
BsaBI GATNNNNATC 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 2 cut(s) 238, 346
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 6 cut(s) 118, 244, 340, 367, 378, 418
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 1 cut(s) 360
Bse8I GATNNNNATC 1 cut(s) 162
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 238, 346
BseGI GGATG 1 cut(s) 256
BseJI GATNNNNATC 1 cut(s) 162
BseLI CCNNNNNNNGG 6 cut(s) 118, 244, 340, 367, 378, 418
BseMII CTCAG 2 cut(s) 30, 407
BseNI ACTGG 1 cut(s) 360
BseRI GAGGAG 1 cut(s) 363
BseSI GKGCMC 1 cut(s) 71
BseXI GCAGC 1 cut(s) 312
BseYI CCCAGC 2 cut(s) 328, 365
Bsh1236I CGCG 3 cut(s) 170, 225, 336
BshFI GGCC 8 cut(s) 35, 54, 69, 236, 333, 345, 360, 371
BshNI GGYRCC 1 cut(s) 266
BsiHKAI GWGCWC 1 cut(s) 309
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 3 cut(s) 17, 273, 372
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 6 cut(s) 118, 244, 340, 367, 378, 418
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 8 cut(s) 35, 54, 69, 236, 333, 345, 360, 371
BsoBI CYCGRG 1 cut(s) 302
Bsp120I GGGCCC 1 cut(s) 67
Bsp1286I GDGCHC 2 cut(s) 71, 309
Bsp143I GATC 4 cut(s) 7, 163, 175, 193
BspANI GGCC 8 cut(s) 35, 54, 69, 236, 333, 345, 360, 371
BspCNI CTCAG 2 cut(s) 31, 408
BspFNI CGCG 3 cut(s) 170, 225, 336
BspLI GGNNCC 4 cut(s) 69, 268, 277, 370
BspT107I GGYRCC 1 cut(s) 266
BsrBI CCGCTC 2 cut(s) 281, 391
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 1 cut(s) 360
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 2 cut(s) 238, 346
BssMI GATC 4 cut(s) 7, 163, 175, 193
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 197
Bst2BI CACGAG 1 cut(s) 197
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 266
BstACI GRCGYC 1 cut(s) 147
BstC8I GCNNGC 2 cut(s) 90, 401
BstDEI CTNAG 2 cut(s) 39, 416
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 256
BstFNI CGCG 3 cut(s) 170, 225, 336
BstH2I RGCGCY 1 cut(s) 302
BstHHI GCGC 3 cut(s) 225, 301, 338
BstKTI GATC 4 cut(s) 10, 166, 178, 196
BstMBI GATC 4 cut(s) 7, 163, 175, 193
BstMWI GCNNNNNNNGC 6 cut(s) 179, 242, 287, 296, 342, 400
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 2 cut(s) 49, 371
BstSLI GKGCMC 1 cut(s) 71
BstUI CGCG 3 cut(s) 170, 225, 336
BstV1I GCAGC 1 cut(s) 312
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuRI GGCC 8 cut(s) 35, 54, 69, 236, 333, 345, 360, 371
BtgI CCRYGG 1 cut(s) 238
BtgZI GCGATG 1 cut(s) 418
BtsCI GGATG 1 cut(s) 256
Cac8I GCNNGC 2 cut(s) 90, 401
CfoI GCGC 3 cut(s) 225, 301, 338
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 7 cut(s) 67, 68, 235, 331, 343, 358, 369
CviAII CATG 1 cut(s) 113
DdeI CTNAG 2 cut(s) 39, 416
DpnI GATC 4 cut(s) 9, 165, 177, 195
DpnII GATC 4 cut(s) 7, 163, 175, 193
DriI GACNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 1 cut(s) 52
Eam1105I GACNNNNNGTC 1 cut(s) 107
EciI GGCGGA 2 cut(s) 197, 391
Ecl136II GAGCTC 1 cut(s) 307
Eco24I GRGCYC 2 cut(s) 71, 309
Eco47III AGCGCT 1 cut(s) 300
Eco53kI GAGCTC 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 302
EcoICRI GAGCTC 1 cut(s) 307
EcoO109I RGGNCCY 2 cut(s) 67, 68
EcoRII CCWGG 1 cut(s) 49
EcoT38I GRGCYC 2 cut(s) 71, 309
FaeI CATG 1 cut(s) 116
FaiI YATR 1 cut(s) 114
FaqI GGGAC 1 cut(s) 395
FatI CATG 1 cut(s) 112
FauI CCCGC 2 cut(s) 112, 420
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 5 cut(s) 36, 171, 326, 334, 392
FokI GGATG 1 cut(s) 263
FriOI GRGCYC 2 cut(s) 71, 309
Fsp4HI GCNGC 5 cut(s) 36, 171, 326, 334, 392
GlaI GCGC 3 cut(s) 224, 300, 337
GluI GCNGC 5 cut(s) 36, 171, 326, 334, 392
GsaI CCCAGC 2 cut(s) 332, 369
GsuI CTGGAG 1 cut(s) 80
HaeII RGCGCY 1 cut(s) 302
HaeIII GGCC 8 cut(s) 35, 54, 69, 236, 333, 345, 360, 371
HapII CCGG 3 cut(s) 17, 273, 372
HhaI GCGC 3 cut(s) 225, 301, 338
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 1 cut(s) 116
Hin6I GCGC 3 cut(s) 223, 299, 336
HinP1I GCGC 3 cut(s) 223, 299, 336
HpaII CCGG 3 cut(s) 17, 273, 372
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 2 cut(s) 62, 311
Hpy188III TCNNGA 2 cut(s) 80, 284
Hpy8I GTNNAC 1 cut(s) 262
Hpy99I CGWCG 2 cut(s) 134, 149
HpyCH4III ACNGT 1 cut(s) 266
HpyCH4IV ACGT 2 cut(s) 132, 147
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 6 cut(s) 179, 242, 287, 296, 342, 400
HpyF3I CTNAG 2 cut(s) 39, 416
HpySE526I ACGT 2 cut(s) 132, 147
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 1 cut(s) 116
HspAI GCGC 3 cut(s) 223, 299, 336
Kzo9I GATC 4 cut(s) 7, 163, 175, 193
LmnI GCTCC 4 cut(s) 281, 286, 296, 388
Lsp1109I GCAGC 1 cut(s) 312
LweI GCATC 1 cut(s) 277
MaeII ACGT 2 cut(s) 132, 147
MaeIII GTNAC 1 cut(s) 148
MalI GATC 4 cut(s) 9, 165, 177, 195
MbiI CCGCTC 2 cut(s) 281, 391
MboI GATC 4 cut(s) 7, 163, 175, 193
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 2 cut(s) 71, 309
MlsI TGGCCA 1 cut(s) 54
MluNI TGGCCA 1 cut(s) 54
MmeI TCCRAC 1 cut(s) 274
Mox20I TGGCCA 1 cut(s) 54
MscI TGGCCA 1 cut(s) 54
Msp20I TGGCCA 1 cut(s) 54
MspA1I CMGCKG 2 cut(s) 38, 415
MspI CCGG 3 cut(s) 17, 273, 372
MspR9I CCNGG 2 cut(s) 51, 373
MvaI CCWGG 1 cut(s) 51
MvnI CGCG 3 cut(s) 170, 225, 336
MwoI GCNNNNNNNGC 6 cut(s) 179, 242, 287, 296, 342, 400
NciI CCSGG 1 cut(s) 373
NdeII GATC 4 cut(s) 7, 163, 175, 193
NlaIII CATG 1 cut(s) 116
NlaIV GGNNCC 4 cut(s) 69, 268, 277, 370
NmeAIII GCCGAG 2 cut(s) 371, 386
NmuCI GTSAC 1 cut(s) 148
PaeR7I CTCGAG 1 cut(s) 302
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 5 cut(s) 37, 172, 327, 335, 393
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 1 cut(s) 49
PspFI CCCAGC 2 cut(s) 328, 365
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 4 cut(s) 69, 268, 277, 370
PspOMI GGGCCC 1 cut(s) 67
PspPI GGNCC 7 cut(s) 67, 68, 235, 331, 343, 358, 369
PspXI VCTCGAGB 1 cut(s) 302
PsuI RGATCY 1 cut(s) 175
SacI GAGCTC 1 cut(s) 309
SatI GCNGC 5 cut(s) 36, 171, 326, 334, 392
Sau3AI GATC 4 cut(s) 7, 163, 175, 193
Sau96I GGNCC 7 cut(s) 67, 68, 235, 331, 343, 358, 369
ScrFI CCNGG 2 cut(s) 51, 373
SduI GDGCHC 2 cut(s) 71, 309
SetI ASST 8 cut(s) 27, 135, 150, 219, 258, 309, 327, 367
SfaNI GCATC 1 cut(s) 277
Sfr274I CTCGAG 1 cut(s) 302
SgrAI CRCCGGYG 1 cut(s) 16
SlaI CTCGAG 1 cut(s) 302
SmlI CTYRAG 2 cut(s) 218, 302
SmoI CTYRAG 2 cut(s) 218, 302
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 2 cut(s) 49, 371
TaaI ACNGT 1 cut(s) 266
TaiI ACGT 2 cut(s) 135, 150
TaqI TCGA 4 cut(s) 144, 162, 192, 303
TauI GCSGC 4 cut(s) 38, 173, 336, 394
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 1 cut(s) 325
Tsp45I GTSAC 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 118
XhoI CTCGAG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 261
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.