pycom17g15390

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0029490
Physical Location & Seq
Forward (+)
13412443 .. 13415034
2592 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 852 bp
ATGGATGTTGATGTGGAGGGTGGTAATTTGGGGCCTTACCAAGACAGGCCGAGGACATTCCCTAACATGCGTGGTAAATCTTACAACCCATTGATTTTCCGGATTCTGATGCGGATAAATGTCCGTGCTCTTTTTGTTATTTTACTTCTTGCACTTGGAGTGGTCTTTTACGTTGGAGCAAGTACTTCCCCTATCATTGTATTCGTCGTCTCAATTTGTATTATCAGCTTTTTGGTGTCTATGTACCTGGCTAAATGGGTTCTCGCAAAGGATGAGGGACCTCCGGAGATGGGACAGATATCAGATGCAATACGTGATGGAGCTGAAGGTTTCTTCAGGACCCAGTATGGAACCATCTCTAAGATGGCAATTTTACTAGCTTCGGTTATCCTCTGCATATATTTATTTCGCAGACCAACACCTCAGCAAGAAGCATCTGGTCTCGGGAGGTTCACTTCAGCTTATATTACGGTAGCTGCATTTCTTTTGGGAGCTTTGTGTTCAGGTGCTGCTGGGTATGTTGGGATGTGGGTTTCAGTTCGTGCAAATGTTCGAGTCTCTAGTGCTGCTAGACGGTCTGCAAGAGAGGCATTACAGATAGCTGTTCGTGCTGGTGGTTTTTCTGCTTTGGTGGTTGTTGGTATGGCTGTACTTGGTATAGCCATCCTGTATGCCATTTTTTATGTTTGGTTGGAGGTGGATTCACCAGGTTCAATGAAGGTTACTGACTTGCCTCTTCTGCTTGTTGGATATGGATTTGGAGCTTCTTTTGTTGCCTTATTTGCTCAGTTGGGAGGTGGAATATACACCAAGGCAGCTGATGTTCGGAGACAGATCTTGTTGGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

284

Amino Acids

30.58

Weight (kDa)

9.61

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 74 - 275 5e-51 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029490)

Species Orthologous Gene IDs
pyrus_communis pycom17g15390
rosa_multiflora Rmu_sc0008040.1_g000014

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 99, 283
AciI CCGC 1 cut(s) 112
AcuI CTGAAG 3 cut(s) 319, 345, 441
AfaI GTAC 3 cut(s) 184, 245, 651
AfiI CCNNNNNNNGG 1 cut(s) 290
AgsI TTSAA 1 cut(s) 714
AjnI CCWGG 2 cut(s) 246, 706
AluBI AGCT 9 cut(s) 228, 323, 380, 461, 476, 494, 602, 764, 818
AluI AGCT 9 cut(s) 228, 323, 380, 461, 476, 494, 602, 764, 818
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 4 cut(s) 214, 446, 562, 823
AlwNI CAGNNNCTG 1 cut(s) 509
Ama87I CYCGRG 1 cut(s) 443
Aor13HI TCCGGA 2 cut(s) 99, 283
AoxI GGCC 2 cut(s) 32, 47
ApeKI GCWGC 4 cut(s) 476, 509, 566, 815
AspS9I GGNCC 3 cut(s) 32, 278, 339
AsuHPI GGTGA 1 cut(s) 696
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 2 cut(s) 278, 339
Bbv12I GWGCWC 1 cut(s) 130
BbvCI CCTCAGC 1 cut(s) 423
BbvI GCAGC 4 cut(s) 463, 496, 553, 827
BccI CCATC 5 cut(s) 283, 311, 358, 362, 671
BciT130I CCWGG 2 cut(s) 248, 708
BcoDI GTCTC 4 cut(s) 214, 446, 562, 823
BfaI CTAG 3 cut(s) 377, 561, 570
BglII AGATCT 1 cut(s) 834
BisI GCNGC 4 cut(s) 477, 510, 567, 816
BlsI GCNGC 4 cut(s) 478, 511, 568, 817
BmcAI AGTACT 1 cut(s) 184
Bme1390I CCNGG 2 cut(s) 248, 708
Bme18I GGWCC 2 cut(s) 278, 339
BmeT110I CYCGRG 1 cut(s) 443
BmgT120I GGNCC 3 cut(s) 32, 278, 339
BmiI GGNNCC 4 cut(s) 33, 279, 341, 352
BmrFI CCNGG 2 cut(s) 248, 708
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 3 cut(s) 99, 295, 443
BmuI ACTGGG 1 cut(s) 337
Bpu10I CCTNAGC 1 cut(s) 423
BsaAI YACGTR 1 cut(s) 314
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 2 cut(s) 50, 810
BsaWI WCCGGW 2 cut(s) 99, 283
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 343
BseAI TCCGGA 2 cut(s) 99, 283
BseBI CCWGG 2 cut(s) 248, 708
BseDI CCNNGG 2 cut(s) 50, 810
BseGI GGATG 4 cut(s) 10, 277, 531, 663
BseLI CCNNNNNNNGG 1 cut(s) 290
BseMII CTCAG 2 cut(s) 437, 800
BseNI ACTGG 1 cut(s) 343
BseXI GCAGC 4 cut(s) 463, 496, 553, 827
BseYI CCCAGC 1 cut(s) 512
BshFI GGCC 2 cut(s) 34, 49
BsiHKAI GWGCWC 1 cut(s) 130
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 2 cut(s) 100, 284
BslFI GGGAC 2 cut(s) 291, 306
BslI CCNNNNNNNGG 1 cut(s) 290
BsmAI GTCTC 4 cut(s) 214, 446, 562, 823
BsmBI CGTCTC 1 cut(s) 214
BsmFI GGGAC 2 cut(s) 291, 306
BsnI GGCC 2 cut(s) 34, 49
Bso31I GGTCTC 1 cut(s) 446
BsoBI CYCGRG 1 cut(s) 443
Bsp1286I GDGCHC 1 cut(s) 130
Bsp13I TCCGGA 2 cut(s) 99, 283
Bsp143I GATC 1 cut(s) 834
BspACI CCGC 1 cut(s) 112
BspANI GGCC 2 cut(s) 34, 49
BspCNI CTCAG 2 cut(s) 436, 799
BspEI TCCGGA 2 cut(s) 99, 283
BspLI GGNNCC 4 cut(s) 33, 279, 341, 352
BspTNI GGTCTC 1 cut(s) 446
BsrI ACTGG 1 cut(s) 343
BssECI CCNNGG 2 cut(s) 50, 810
BssMI GATC 1 cut(s) 834
BssT1I CCWWGG 1 cut(s) 810
Bst2UI CCWGG 2 cut(s) 248, 708
Bst4CI ACNGT 2 cut(s) 472, 576
Bst6I CTCTTC 1 cut(s) 741
BstBAI YACGTR 1 cut(s) 314
BstDEI CTNAG 3 cut(s) 360, 423, 786
BstF5I GGATG 4 cut(s) 10, 277, 531, 663
BstKTI GATC 1 cut(s) 837
BstMAI GTCTC 4 cut(s) 214, 446, 562, 823
BstMBI GATC 1 cut(s) 834
BstMWI GCNNNNNNNGC 4 cut(s) 587, 608, 739, 782
BstNI CCWGG 2 cut(s) 248, 708
BstNSI RCATGY 1 cut(s) 70
BstSCI CCNGG 2 cut(s) 246, 706
BstV1I GCAGC 4 cut(s) 463, 496, 553, 827
BstX2I RGATCY 1 cut(s) 834
BstYI RGATCY 1 cut(s) 834
BsuRI GGCC 2 cut(s) 34, 49
BtsCI GGATG 4 cut(s) 10, 277, 531, 663
CaiI CAGNNNCTG 1 cut(s) 509
Cfr13I GGNCC 3 cut(s) 32, 278, 339
CsiI ACCWGGT 1 cut(s) 706
Csp6I GTAC 3 cut(s) 183, 244, 650
CviAII CATG 1 cut(s) 67
CviQI GTAC 3 cut(s) 183, 244, 650
DdeI CTNAG 3 cut(s) 360, 423, 786
DpnI GATC 1 cut(s) 836
DpnII GATC 1 cut(s) 834
Eam1104I CTCTTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 741
Eco130I CCWWGG 1 cut(s) 810
Eco31I GGTCTC 1 cut(s) 446
Eco32I GATATC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 278, 339
Eco57I CTGAAG 3 cut(s) 319, 345, 441
Eco88I CYCGRG 1 cut(s) 443
EcoO109I RGGNCCY 3 cut(s) 32, 278, 339
EcoRII CCWGG 2 cut(s) 246, 706
EcoRV GATATC 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 810
ErhI CCWWGG 1 cut(s) 810
Esp3I CGTCTC 1 cut(s) 214
FaeI CATG 1 cut(s) 70
FaqI GGGAC 2 cut(s) 291, 306
FatI CATG 1 cut(s) 66
Fnu4HI GCNGC 4 cut(s) 477, 510, 567, 816
FokI GGATG 4 cut(s) 17, 284, 538, 650
Fsp4HI GCNGC 4 cut(s) 477, 510, 567, 816
FspBI CTAG 3 cut(s) 377, 561, 570
GluI GCNGC 4 cut(s) 477, 510, 567, 816
GsaI CCCAGC 1 cut(s) 516
HaeIII GGCC 2 cut(s) 34, 49
HapII CCGG 2 cut(s) 100, 284
Hin1II CATG 1 cut(s) 70
HinfI GANTC 3 cut(s) 103, 555, 701
HpaII CCGG 2 cut(s) 100, 284
HphI GGTGA 1 cut(s) 696
Hpy166II GTNNAC 1 cut(s) 453
Hpy188I TCNGA 3 cut(s) 108, 304, 828
Hpy188III TCNNGA 4 cut(s) 100, 284, 337, 445
Hpy8I GTNNAC 1 cut(s) 453
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 320, 712
HpyCH4III ACNGT 2 cut(s) 472, 576
HpyCH4IV ACGT 2 cut(s) 171, 313
HpyCH4V TGCA 6 cut(s) 152, 308, 396, 479, 545, 581
HpyF10VI GCNNNNNNNGC 4 cut(s) 587, 608, 739, 782
HpyF3I CTNAG 3 cut(s) 360, 423, 786
HpySE526I ACGT 2 cut(s) 171, 313
Hsp92II CATG 1 cut(s) 70
Kpn2I TCCGGA 2 cut(s) 99, 283
Kzo9I GATC 1 cut(s) 834
LmnI GCTCC 4 cut(s) 176, 320, 491, 761
Lsp1109I GCAGC 4 cut(s) 463, 496, 553, 827
LweI GCATC 3 cut(s) 99, 295, 443
MabI ACCWGGT 1 cut(s) 706
MaeI CTAG 3 cut(s) 377, 561, 570
MaeII ACGT 2 cut(s) 171, 313
MaeIII GTNAC 1 cut(s) 721
MalI GATC 1 cut(s) 836
MboI GATC 1 cut(s) 834
MboII GAAGA 2 cut(s) 325, 728
MflI RGATCY 1 cut(s) 834
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 3 cut(s) 25, 213, 369
MlyI GAGTC 1 cut(s) 564
MmeI TCCRAC 4 cut(s) 154, 672, 727, 822
MroI TCCGGA 2 cut(s) 99, 283
MspA1I CMGCKG 1 cut(s) 818
MspI CCGG 2 cut(s) 100, 284
MspR9I CCNGG 2 cut(s) 248, 708
MvaI CCWGG 2 cut(s) 248, 708
MwoI GCNNNNNNNGC 4 cut(s) 587, 608, 739, 782
NdeII GATC 1 cut(s) 834
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 4 cut(s) 33, 279, 341, 352
NmeAIII GCCGAG 1 cut(s) 75
NspI RCATGY 1 cut(s) 70
PfeI GAWTC 2 cut(s) 103, 701
PkrI GCNGC 4 cut(s) 478, 511, 568, 817
PleI GAGTC 1 cut(s) 563
PpsI GAGTC 1 cut(s) 563
Ppu21I YACGTR 1 cut(s) 314
PpuMI RGGWCCY 2 cut(s) 278, 339
Psp5II RGGWCCY 2 cut(s) 278, 339
Psp6I CCWGG 2 cut(s) 246, 706
PspFI CCCAGC 1 cut(s) 512
PspGI CCWGG 2 cut(s) 246, 706
PspN4I GGNNCC 4 cut(s) 33, 279, 341, 352
PspPI GGNCC 3 cut(s) 32, 278, 339
PspPPI RGGWCCY 2 cut(s) 278, 339
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 1 cut(s) 834
PvuII CAGCTG 1 cut(s) 818
RsaI GTAC 3 cut(s) 184, 245, 651
RsaNI GTAC 3 cut(s) 183, 244, 650
SatI GCNGC 4 cut(s) 477, 510, 567, 816
Sau3AI GATC 1 cut(s) 834
Sau96I GGNCC 3 cut(s) 32, 278, 339
ScaI AGTACT 1 cut(s) 184
SchI GAGTC 1 cut(s) 564
ScrFI CCNGG 2 cut(s) 248, 708
SduI GDGCHC 1 cut(s) 130
SexAI ACCWGGT 1 cut(s) 706
SfaNI GCATC 3 cut(s) 99, 295, 443
SinI GGWCC 2 cut(s) 278, 339
Sse9I AATT 3 cut(s) 25, 213, 369
SsiI CCGC 1 cut(s) 112
SspMI CTAG 3 cut(s) 377, 561, 570
StyD4I CCNGG 2 cut(s) 246, 706
StyI CCWWGG 1 cut(s) 810
TaaI ACNGT 2 cut(s) 472, 576
TaiI ACGT 2 cut(s) 174, 316
TaqI TCGA 1 cut(s) 553
TasI AATT 3 cut(s) 25, 213, 369
TatI WGTACW 2 cut(s) 182, 649
TfiI GAWTC 2 cut(s) 103, 701
TseI GCWGC 4 cut(s) 476, 509, 566, 815
TspDTI ATGAA 1 cut(s) 731
TspGWI ACGGA 1 cut(s) 113
VpaK11BI GGWCC 2 cut(s) 278, 339
XceI RCATGY 1 cut(s) 70
XcmI CCANNNNNNNNNTGG 1 cut(s) 361
XspI CTAG 3 cut(s) 377, 561, 570
ZrmI AGTACT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.