pycom17g23380

Domain associated at C-terminal with AAA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0029493
Physical Location & Seq
Forward (+)
21735611 .. 21736078
468 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 468 bp
ATGGACGAGCTGTTGAATTCGTGTTGTGCCGAGGAGAGAGTCATGGTGTTCACCATGAATTCGAAATACCTCTTTAACCCGGCCTTTCTTCGACCGGGTCGGATCGACGTCCACATCCACTTTCCACTCTACGATTTTGGAGCATTCAAAAATCTGGCGACGAGTTATCTCAGGGTGAAGGAGCACAAGCTCTTCCCACAGGTGAAGGAGATTTTCCTAAGCAGGTCGAGTTTGAGTCCGGTGAAGATCGGCGAGTTGATGATCGTGAACCGGAACTTGCTGAGTAGGGCGATTAAGTCGGTCATCACAGCTTTGCAAACGGACAGCAATGGGAGGGGCGCGGGAGTGTTCGGAAGGCAGGTTAGGTTTGATACCGAGTCAAGAAAGTCCGAATCGGGTGAACCCGGTTGGGCGTTTAAGGGGAAAGGCGGTGTTCCTACGGTTAGGGATATTAGGAAGTGTTGGTGA

Protein Analysis

156

Amino Acids

17.57

Weight (kDa)

9.79

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_lid_At3g28540 PF25568 46 - 108 1.1e-07 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0029493)

Species Orthologous Gene IDs
pyrus_communis pycom17g23380
rosa_chinensis RchiOBHm_Chr3g0458201

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 111
Acc36I ACCTGC 2 cut(s) 213, 349
AccII CGCG 1 cut(s) 341
AciI CCGC 2 cut(s) 341, 429
AclWI GGATC 1 cut(s) 110
AcsI RAATTY 2 cut(s) 16, 58
AcyI GRCGYC 1 cut(s) 108
AgsI TTSAA 2 cut(s) 16, 148
AluBI AGCT 3 cut(s) 10, 190, 311
AluI AGCT 3 cut(s) 10, 190, 311
Alw21I GWGCWC 1 cut(s) 186
AlwI GGATC 1 cut(s) 110
AoxI GGCC 1 cut(s) 81
ApoI RAATTY 2 cut(s) 16, 58
AspLEI GCGC 1 cut(s) 341
AsuC2I CCSGG 3 cut(s) 80, 96, 405
AsuHPI GGTGA 5 cut(s) 43, 187, 214, 253, 410
AsuII TTCGAA 1 cut(s) 62
Bbv12I GWGCWC 1 cut(s) 186
BcnI CCSGG 3 cut(s) 80, 96, 405
BfuAI ACCTGC 2 cut(s) 213, 349
Bme1390I CCNGG 3 cut(s) 80, 96, 405
BmrFI CCNGG 3 cut(s) 80, 96, 405
Bpu10I CCTNAGC 1 cut(s) 218
Bpu14I TTCGAA 1 cut(s) 62
BpuMI CCSGG 3 cut(s) 80, 96, 405
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 2 cut(s) 238, 270
Bse3DI GCAATG 1 cut(s) 334
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 114
BseMI GCAATG 1 cut(s) 334
BseMII CTCAG 2 cut(s) 184, 272
BseRI GAGGAG 1 cut(s) 47
Bsh1236I CGCG 1 cut(s) 341
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 1 cut(s) 83
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 5 cut(s) 80, 95, 239, 271, 405
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 1 cut(s) 83
Bsp119I TTCGAA 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 3 cut(s) 102, 246, 261
BspACI CCGC 2 cut(s) 341, 429
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 2 cut(s) 183, 273
BspFNI CGCG 1 cut(s) 341
BspMI ACCTGC 2 cut(s) 213, 349
BspPI GGATC 1 cut(s) 110
BspQI GCTCTTC 1 cut(s) 197
BspT104I TTCGAA 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 334
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 3 cut(s) 102, 246, 261
BssNI GRCGYC 1 cut(s) 108
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 1 cut(s) 108
BstBI TTCGAA 1 cut(s) 62
BstDEI CTNAG 3 cut(s) 170, 218, 281
BstF5I GGATG 1 cut(s) 114
BstFNI CGCG 1 cut(s) 341
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 3 cut(s) 105, 249, 264
BstMBI GATC 3 cut(s) 102, 246, 261
BstMCI CGRYCG 1 cut(s) 95
BstSCI CCNGG 3 cut(s) 78, 94, 403
BstUI CGCG 1 cut(s) 341
BsuRI GGCC 1 cut(s) 83
BtsCI GGATG 1 cut(s) 114
BveI ACCTGC 2 cut(s) 213, 349
CfoI GCGC 1 cut(s) 341
CviAII CATG 2 cut(s) 43, 55
CviJI RGCY 4 cut(s) 10, 83, 190, 311
CviKI_1 RGCY 4 cut(s) 10, 83, 190, 311
DdeI CTNAG 3 cut(s) 170, 218, 281
DpnI GATC 3 cut(s) 104, 248, 263
DpnII GATC 3 cut(s) 102, 246, 261
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
EcoRI GAATTC 2 cut(s) 16, 58
FaeI CATG 2 cut(s) 46, 58
FaiI YATR 2 cut(s) 44, 56
FatI CATG 2 cut(s) 42, 54
FauI CCCGC 1 cut(s) 334
FokI GGATG 1 cut(s) 101
GlaI GCGC 1 cut(s) 340
HaeIII GGCC 1 cut(s) 83
HapII CCGG 5 cut(s) 80, 95, 239, 271, 405
HhaI GCGC 1 cut(s) 341
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 2 cut(s) 46, 58
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HinfI GANTC 4 cut(s) 39, 235, 377, 392
HpaII CCGG 5 cut(s) 80, 95, 239, 271, 405
HphI GGTGA 5 cut(s) 43, 187, 214, 253, 410
Hpy166II GTNNAC 4 cut(s) 51, 112, 268, 401
Hpy188I TCNGA 3 cut(s) 102, 353, 391
Hpy188III TCNNGA 2 cut(s) 265, 381
Hpy8I GTNNAC 4 cut(s) 51, 112, 268, 401
Hpy99I CGWCG 2 cut(s) 110, 163
HpyAV CCTTC 3 cut(s) 172, 199, 348
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 1 cut(s) 316
HpyF3I CTNAG 3 cut(s) 170, 218, 281
HpySE526I ACGT 1 cut(s) 108
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 2 cut(s) 46, 58
HspAI GCGC 1 cut(s) 339
Kzo9I GATC 3 cut(s) 102, 246, 261
LguI GCTCTTC 1 cut(s) 197
LmnI GCTCC 2 cut(s) 140, 181
MaeII ACGT 1 cut(s) 108
MalI GATC 3 cut(s) 104, 248, 263
MboI GATC 3 cut(s) 102, 246, 261
MboII GAAGA 3 cut(s) 80, 184, 256
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 2 cut(s) 16, 58
MlyI GAGTC 3 cut(s) 48, 244, 386
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 3 cut(s) 25, 80, 327
MseI TTAA 3 cut(s) 75, 294, 417
MspI CCGG 5 cut(s) 80, 95, 239, 271, 405
MspR9I CCNGG 3 cut(s) 80, 96, 405
Mva1269I GAATGC 1 cut(s) 143
MvnI CGCG 1 cut(s) 341
NciI CCSGG 3 cut(s) 80, 96, 405
NdeII GATC 3 cut(s) 102, 246, 261
NlaIII CATG 2 cut(s) 46, 58
NmeAIII GCCGAG 1 cut(s) 55
NspV TTCGAA 1 cut(s) 62
PciSI GCTCTTC 1 cut(s) 197
PctI GAATGC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 392
PflFI GACNNNGTC 1 cut(s) 96
PleI GAGTC 3 cut(s) 47, 243, 385
PpsI GAGTC 3 cut(s) 47, 243, 385
PsyI GACNNNGTC 1 cut(s) 96
SapI GCTCTTC 1 cut(s) 197
SaqAI TTAA 3 cut(s) 75, 294, 417
Sau3AI GATC 3 cut(s) 102, 246, 261
SchI GAGTC 3 cut(s) 48, 244, 386
ScrFI CCNGG 3 cut(s) 80, 96, 405
SduI GDGCHC 1 cut(s) 186
SetI ASST 9 cut(s) 12, 72, 111, 192, 204, 227, 313, 363, 368
SfuI TTCGAA 1 cut(s) 62
Sse9I AATT 2 cut(s) 16, 58
SsiI CCGC 2 cut(s) 341, 429
StyD4I CCNGG 3 cut(s) 78, 94, 403
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 1 cut(s) 111
TaqI TCGA 4 cut(s) 62, 91, 105, 227
TaqII GACCGA 1 cut(s) 289
TasI AATT 2 cut(s) 16, 58
TfiI GAWTC 1 cut(s) 392
Tru1I TTAA 3 cut(s) 75, 294, 417
Tru9I TTAA 3 cut(s) 75, 294, 417
TspDTI ATGAA 1 cut(s) 71
TspGWI ACGGA 1 cut(s) 335
Tth111I GACNNNGTC 1 cut(s) 96
XapI RAATTY 2 cut(s) 16, 58
ZraI GACGTC 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.