pycom17g25990

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0020829
Physical Location & Seq
Forward (+)
24304255 .. 24304719
465 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 465 bp
ATGTCAAAGAGAGAATTGGTCTCGAAAGGCTCGGAAGACGGCGCCGCCGGCTCCATGGAGGAGCTGAAGAAGGTCTTCGACAAGTTCGACAAGAACGGCGACGGCAGGATCGCCTGCGACGAGCTCAGGGGCGTTTTCGGCGATCTCGGTTCCGAGACGGTCTCACTGGAGGAGGTCCAGCGCATTATGGCTGAGTTCGACAAGGACGGGGACGGTCACATTGACATCGTGGAGTTCGCAGAGATCATCAATGGCGGATCCACCAAGGAGCTTCGCGACGCGTTCGACCTCTACGATCTCGACAAGAACGGGCTGATCTCCACGAGTGAGCTGCATGAGGTGCTGAAGCGGTTGGGGCAGAAGTGCTCGCTCAAGGACTGCGCGAAGATGATCAGTTCCGTCGACGCCGACGGCGATGGCCACGTCAACTTCGCCGAGTTCAAGAAGATGATGACTCGCAAATAA

Protein Analysis

155

Amino Acids

17.0

Weight (kDa)

4.82

Isoelectric Point (pI)

35.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 20 - 82 4.9e-14 EF-hand domain pair
EF-hand_1 PF00036 21 - 46 4.8e-06 EF hand domain
EF-hand_6 PF13405 21 - 50 9.2e-07 EF-hand domain
EF-hand_1 PF00036 58 - 83 2.9e-07 EF hand domain
EF-hand_7 PF13499 89 - 152 9.5e-15 EF-hand domain pair
EF-hand_1 PF00036 90 - 118 6.5e-07 EF hand domain
EF-hand_6 PF13405 90 - 119 2.2e-07 EF-hand domain
EF-hand_8 PF13833 116 - 153 2.9e-06 EF-hand domain pair
EF-hand_1 PF00036 127 - 153 3.1e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020829)

Species Orthologous Gene IDs
malus_domestica MD09G1262900.v1.1 MD17G1257900.v1.1
prunus_persica Prupe.3G160600_v2.0.a1
pyrus_communis pycom09g17720 pycom17g25990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 41
AccI GTMKAC 1 cut(s) 402
AccII CGCG 3 cut(s) 276, 281, 383
AciI CCGC 3 cut(s) 45, 255, 349
AclWI GGATC 3 cut(s) 116, 252, 265
AcoI YGGCCR 1 cut(s) 418
AcuI CTGAAG 2 cut(s) 86, 365
AcyI GRCGYC 2 cut(s) 42, 405
AflIII ACRYGT 1 cut(s) 279
AgsI TTSAA 1 cut(s) 442
AjiI CACGTC 1 cut(s) 424
AluBI AGCT 4 cut(s) 64, 124, 271, 331
AluI AGCT 4 cut(s) 64, 124, 271, 331
Alw21I GWGCWC 2 cut(s) 126, 368
Alw26I GTCTC 3 cut(s) 25, 149, 166
AlwI GGATC 3 cut(s) 116, 252, 265
AoxI GGCC 1 cut(s) 418
ApeKI GCWGC 1 cut(s) 331
Asp700I GAANNNNTTC 1 cut(s) 74
AspLEI GCGC 3 cut(s) 44, 183, 383
AspS9I GGNCC 1 cut(s) 175
AvaII GGWCC 1 cut(s) 175
BalI TGGCCA 1 cut(s) 420
BamHI GGATCC 1 cut(s) 257
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 126
BauI CACGAG 1 cut(s) 322
BbsI GAAGAC 2 cut(s) 42, 67
Bbv12I GWGCWC 2 cut(s) 126, 368
BbvI GCAGC 1 cut(s) 318
BccI CCATC 1 cut(s) 410
BceAI ACGGC 4 cut(s) 55, 112, 118, 427
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BclI TGATCA 1 cut(s) 390
BcoDI GTCTC 3 cut(s) 25, 149, 166
BfoI RGCGCY 1 cut(s) 45
BisI GCNGC 2 cut(s) 45, 332
BlsI GCNGC 2 cut(s) 46, 333
Bme18I GGWCC 1 cut(s) 175
BmgBI CACGTC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 4 cut(s) 43, 52, 151, 259
BpiI GAAGAC 2 cut(s) 42, 67
BplI GAGNNNNNCTC 2 cut(s) 146, 178
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 356
BsaHI GRCGYC 2 cut(s) 42, 405
BsaI GGTCTC 2 cut(s) 25, 166
BsaJI CCNNGG 2 cut(s) 54, 264
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 171
BseDI CCNNGG 2 cut(s) 54, 264
BseMII CTCAG 2 cut(s) 139, 183
BseNI ACTGG 1 cut(s) 171
BseRI GAGGAG 2 cut(s) 74, 185
BseXI GCAGC 1 cut(s) 318
Bsh1236I CGCG 3 cut(s) 276, 281, 383
BshFI GGCC 1 cut(s) 420
BshNI GGYRCC 1 cut(s) 41
BsiHKAI GWGCWC 2 cut(s) 126, 368
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 1 cut(s) 224
BsmAI GTCTC 3 cut(s) 25, 149, 166
BsmBI CGTCTC 1 cut(s) 149
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 1 cut(s) 420
Bso31I GGTCTC 2 cut(s) 25, 166
Bsp1286I GDGCHC 2 cut(s) 126, 368
Bsp143I GATC 7 cut(s) 108, 142, 243, 257, 295, 315, 390
Bsp19I CCATGG 1 cut(s) 54
Bsp68I TCGCGA 1 cut(s) 276
BspACI CCGC 3 cut(s) 45, 255, 349
BspANI GGCC 1 cut(s) 420
BspCNI CTCAG 2 cut(s) 138, 184
BspFNI CGCG 3 cut(s) 276, 281, 383
BspLI GGNNCC 4 cut(s) 43, 52, 151, 259
BspPI GGATC 3 cut(s) 116, 252, 265
BspT107I GGYRCC 1 cut(s) 41
BspTNI GGTCTC 2 cut(s) 25, 166
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 1 cut(s) 171
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 2 cut(s) 54, 264
BssMI GATC 7 cut(s) 108, 142, 243, 257, 295, 315, 390
BssNI GRCGYC 2 cut(s) 42, 405
BssSI CACGAG 1 cut(s) 322
BssT1I CCWWGG 2 cut(s) 54, 264
Bst2BI CACGAG 1 cut(s) 322
Bst4CI ACNGT 2 cut(s) 160, 215
BstACI GRCGYC 2 cut(s) 42, 405
BstAPI GCANNNNNTGC 1 cut(s) 340
BstC8I GCNNGC 3 cut(s) 49, 115, 368
BstDEI CTNAG 2 cut(s) 125, 192
BstDSI CCRYGG 1 cut(s) 54
BstFNI CGCG 3 cut(s) 276, 281, 383
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 3 cut(s) 44, 183, 383
BstKTI GATC 7 cut(s) 111, 145, 246, 260, 298, 318, 393
BstMAI GTCTC 3 cut(s) 25, 149, 166
BstMBI GATC 7 cut(s) 108, 142, 243, 257, 295, 315, 390
BstMWI GCNNNNNNNGC 4 cut(s) 48, 138, 340, 355
BstUI CGCG 3 cut(s) 276, 281, 383
BstV1I GCAGC 1 cut(s) 318
BstV2I GAAGAC 2 cut(s) 42, 67
BstX2I RGATCY 1 cut(s) 257
BstYI RGATCY 1 cut(s) 257
BsuRI GGCC 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 54
BtgZI GCGATG 1 cut(s) 429
BtrI CACGTC 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 164
BtuMI TCGCGA 1 cut(s) 276
Cac8I GCNNGC 3 cut(s) 49, 115, 368
CfoI GCGC 3 cut(s) 44, 183, 383
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 1 cut(s) 175
CseI GACGC 2 cut(s) 287, 413
CviAII CATG 2 cut(s) 55, 335
CviJI RGCY 9 cut(s) 30, 51, 64, 124, 191, 271, 313, 331, 420
CviKI_1 RGCY 9 cut(s) 30, 51, 64, 124, 191, 271, 313, 331, 420
DdeI CTNAG 2 cut(s) 125, 192
DinI GGCGCC 1 cut(s) 43
DpnI GATC 7 cut(s) 110, 144, 245, 259, 297, 317, 392
DpnII GATC 7 cut(s) 108, 142, 243, 257, 295, 315, 390
EaeI YGGCCR 1 cut(s) 418
EciI GGCGGA 1 cut(s) 270
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 2 cut(s) 54, 264
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 2 cut(s) 25, 166
Eco47I GGWCC 1 cut(s) 175
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 2 cut(s) 86, 365
EcoICRI GAGCTC 1 cut(s) 124
EcoT14I CCWWGG 2 cut(s) 54, 264
EcoT38I GRGCYC 1 cut(s) 126
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
ErhI CCWWGG 2 cut(s) 54, 264
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 2 cut(s) 58, 338
FaiI YATR 3 cut(s) 56, 188, 336
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FaqI GGGAC 1 cut(s) 224
FatI CATG 2 cut(s) 54, 334
FbaI TGATCA 1 cut(s) 390
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 2 cut(s) 45, 332
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 2 cut(s) 45, 332
GlaI GCGC 3 cut(s) 43, 182, 382
GluI GCNGC 2 cut(s) 45, 332
GsuI CTGGAG 1 cut(s) 188
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 1 cut(s) 420
HapII CCGG 1 cut(s) 48
HgaI GACGC 2 cut(s) 287, 413
HhaI GCGC 3 cut(s) 44, 183, 383
Hin1I GRCGYC 2 cut(s) 42, 405
Hin1II CATG 2 cut(s) 58, 338
Hin6I GCGC 3 cut(s) 42, 181, 381
HinP1I GCGC 3 cut(s) 42, 181, 381
HincII GTYRAC 2 cut(s) 403, 427
HindII GTYRAC 2 cut(s) 403, 427
HinfI GANTC 1 cut(s) 454
HpaII CCGG 1 cut(s) 48
Hpy166II GTNNAC 2 cut(s) 403, 427
Hpy188I TCNGA 2 cut(s) 34, 154
Hpy188III TCNNGA 4 cut(s) 22, 275, 299, 442
Hpy8I GTNNAC 2 cut(s) 403, 427
Hpy99I CGWCG 6 cut(s) 104, 122, 281, 404, 407, 413
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 2 cut(s) 160, 215
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 1 cut(s) 334
HpyF10VI GCNNNNNNNGC 4 cut(s) 48, 138, 340, 355
HpyF3I CTNAG 2 cut(s) 125, 192
HpySE526I ACGT 1 cut(s) 423
Hsp92I GRCGYC 2 cut(s) 42, 405
Hsp92II CATG 2 cut(s) 58, 338
HspAI GCGC 3 cut(s) 42, 181, 381
KasI GGCGCC 1 cut(s) 41
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
Ksp22I TGATCA 1 cut(s) 390
Kzo9I GATC 7 cut(s) 108, 142, 243, 257, 295, 315, 390
LmnI GCTCC 3 cut(s) 56, 61, 268
LpnPI CCDG 6 cut(s) 61, 91, 112, 127, 152, 191
Lsp1109I GCAGC 1 cut(s) 318
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 1 cut(s) 215
MalI GATC 7 cut(s) 110, 144, 245, 259, 297, 317, 392
MboI GATC 7 cut(s) 108, 142, 243, 257, 295, 315, 390
MboII GAAGA 5 cut(s) 47, 67, 79, 397, 457
MflI RGATCY 1 cut(s) 257
MhlI GDGCHC 2 cut(s) 126, 368
MlsI TGGCCA 1 cut(s) 420
MluCI AATT 1 cut(s) 14
MluI ACGCGT 1 cut(s) 279
MluNI TGGCCA 1 cut(s) 420
Mly113I GGCGCC 1 cut(s) 42
MlyI GAGTC 1 cut(s) 448
MnlI CCTC 5 cut(s) 52, 163, 166, 299, 331
Mox20I TGGCCA 1 cut(s) 420
MroNI GCCGGC 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 74
MscI TGGCCA 1 cut(s) 420
Msp20I TGGCCA 1 cut(s) 420
MspI CCGG 1 cut(s) 48
MvnI CGCG 3 cut(s) 276, 281, 383
MwoI GCNNNNNNNGC 4 cut(s) 48, 138, 340, 355
NaeI GCCGGC 1 cut(s) 49
NarI GGCGCC 1 cut(s) 42
NcoI CCATGG 1 cut(s) 54
NdeII GATC 7 cut(s) 108, 142, 243, 257, 295, 315, 390
NgoMIV GCCGGC 1 cut(s) 47
NlaIII CATG 2 cut(s) 58, 338
NlaIV GGNNCC 4 cut(s) 43, 52, 151, 259
NmeAIII GCCGAG 1 cut(s) 460
NmuCI GTSAC 1 cut(s) 215
NruI TCGCGA 1 cut(s) 276
PcsI WCGNNNNNNNCGW 4 cut(s) 84, 117, 291, 411
PdiI GCCGGC 1 cut(s) 49
PdmI GAANNNNTTC 1 cut(s) 74
PkrI GCNGC 2 cut(s) 46, 333
PleI GAGTC 1 cut(s) 448
PluTI GGCGCC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 448
Psp124BI GAGCTC 1 cut(s) 126
PspN4I GGNNCC 4 cut(s) 43, 52, 151, 259
PspPI GGNCC 1 cut(s) 175
PsuI RGATCY 1 cut(s) 257
RruI TCGCGA 1 cut(s) 276
SacI GAGCTC 1 cut(s) 126
SalI GTCGAC 1 cut(s) 401
SatI GCNGC 2 cut(s) 45, 332
Sau3AI GATC 7 cut(s) 108, 142, 243, 257, 295, 315, 390
Sau96I GGNCC 1 cut(s) 175
SchI GAGTC 1 cut(s) 448
SduI GDGCHC 2 cut(s) 126, 368
SetI ASST 9 cut(s) 66, 75, 126, 177, 273, 291, 333, 342, 426
SfoI GGCGCC 1 cut(s) 43
SgrDI CGTCGACG 1 cut(s) 401
SinI GGWCC 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 1 cut(s) 14
SsiI CCGC 3 cut(s) 45, 255, 349
SspDI GGCGCC 1 cut(s) 41
SstI GAGCTC 1 cut(s) 126
StyI CCWWGG 2 cut(s) 54, 264
TaaI ACNGT 2 cut(s) 160, 215
TaiI ACGT 1 cut(s) 426
TaqI TCGA 7 cut(s) 23, 78, 87, 198, 285, 300, 402
TasI AATT 1 cut(s) 14
TauI GCSGC 1 cut(s) 47
TscAI CASTG 1 cut(s) 171
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 1 cut(s) 331
Tsp45I GTSAC 1 cut(s) 215
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 1 cut(s) 171
VpaK11BI GGWCC 1 cut(s) 175
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.