pycom461g00110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000461
OG0019728
Physical Location & Seq
Forward (+)
77954 .. 78442
489 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGCCATTCGGAAGAAGTCTTCTACAGAGGGAAAGCCTGTTACGAGTAAGTGGAGAGGAAAGATCTCCAGAGATTCTTATCTCATTCCATTCCAGTGGCTCGACCAGTAACCAATGGCGAAAACTAAAAAATCCATGGTTTCCCGGTAGAACCCCATTTCGCCCAAGTTGGTGCGAAACCGGAAAAAAGAAGCGGTTTTTCGCACAGCTTGCTCATAGTGCAGGTCCCACTTGTATATCGTATTTGGCCGAAGAAGCATCGGACAGGTTGGAGTTCTTACCTTCTTGGGACTCCATGGACCAAGATCTGCTTTCATTATATGGGCAATACCGATCTACTTTAGTAGATCATATGGATGTAGAAAAAGCTTATGATTTTGATGAAATGGAAACATCTCTTTTCCATTTCTATTTACCTAGTTCATATCTTTGTTTCGTGTGTTCCCGTGAGGAATTCGATCTCTTCAATCTCGGGATACCGCCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

18.84

Weight (kDa)

5.58

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019728)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g21890
pyrus_communis pycom461g00110
rosa_chinensis RchiOBHm_MTg0499041
rosa_laevigata RLG00000000411 RLG00000000423
rosa_roxburghii Rroxscaffold_2G00119490
rosa_samantha Rh1BG018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 212
AciI CCGC 2 cut(s) 193, 479
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 1 cut(s) 452
AgsI TTSAA 1 cut(s) 466
AluBI AGCT 2 cut(s) 208, 368
AluI AGCT 2 cut(s) 208, 368
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 1 cut(s) 246
ApoI RAATTY 1 cut(s) 452
AspS9I GGNCC 2 cut(s) 224, 298
AsuC2I CCSGG 1 cut(s) 144
AvaI CYCGRG 1 cut(s) 470
AvaII GGWCC 2 cut(s) 224, 298
BbsI GAAGAC 1 cut(s) 11
BciVI GTATCC 1 cut(s) 468
BcnI CCSGG 1 cut(s) 144
BfaI CTAG 1 cut(s) 417
BfmI CTRYAG 1 cut(s) 23
BfuAI ACCTGC 1 cut(s) 212
BfuI GTATCC 1 cut(s) 468
BglII AGATCT 2 cut(s) 62, 304
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 2 cut(s) 224, 298
BmeT110I CYCGRG 1 cut(s) 470
BmgT120I GGNCC 2 cut(s) 224, 298
BmiI GGNNCC 1 cut(s) 226
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 266
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 51
BpuMI CCSGG 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 2 cut(s) 134, 294
BsaWI WCCGGW 1 cut(s) 179
Bse1I ACTGG 2 cut(s) 93, 105
Bse8I GATNNNNATC 1 cut(s) 77
BseDI CCNNGG 2 cut(s) 134, 294
BseGI GGATG 1 cut(s) 361
BseJI GATNNNNATC 1 cut(s) 77
BseNI ACTGG 2 cut(s) 93, 105
BsgI GTGCAG 1 cut(s) 240
BshFI GGCC 1 cut(s) 248
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 2 cut(s) 144, 180
BslFI GGGAC 2 cut(s) 210, 302
BsmFI GGGAC 2 cut(s) 210, 302
BsnI GGCC 1 cut(s) 248
BsoBI CYCGRG 1 cut(s) 470
Bsp143I GATC 5 cut(s) 62, 304, 332, 346, 457
Bsp19I CCATGG 2 cut(s) 134, 294
BspACI CCGC 2 cut(s) 193, 479
BspANI GGCC 1 cut(s) 248
BspLI GGNNCC 1 cut(s) 226
BspMI ACCTGC 1 cut(s) 212
BsrI ACTGG 2 cut(s) 93, 105
BssECI CCNNGG 2 cut(s) 134, 294
BssMI GATC 5 cut(s) 62, 304, 332, 346, 457
BssT1I CCWWGG 2 cut(s) 134, 294
Bst6I CTCTTC 1 cut(s) 467
BstAPI GCANNNNNTGC 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 210
BstDSI CCRYGG 2 cut(s) 134, 294
BstF5I GGATG 1 cut(s) 361
BstKTI GATC 5 cut(s) 65, 307, 335, 349, 460
BstMBI GATC 5 cut(s) 62, 304, 332, 346, 457
BstMWI GCNNNNNNNGC 3 cut(s) 209, 218, 254
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 1 cut(s) 23
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 2 cut(s) 62, 304
BstXI CCANNNNNNTGG 1 cut(s) 95
BstYI RGATCY 2 cut(s) 62, 304
BsuI GTATCC 1 cut(s) 468
BsuRI GGCC 1 cut(s) 248
BtgI CCRYGG 2 cut(s) 134, 294
BtsCI GGATG 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 100
BveI ACCTGC 1 cut(s) 212
Cac8I GCNNGC 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 224, 298
CviAII CATG 2 cut(s) 135, 295
CviJI RGCY 5 cut(s) 36, 99, 208, 248, 368
CviKI_1 RGCY 5 cut(s) 36, 99, 208, 248, 368
DpnI GATC 5 cut(s) 64, 306, 334, 348, 459
DpnII GATC 5 cut(s) 62, 304, 332, 346, 457
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 467
Eco130I CCWWGG 2 cut(s) 134, 294
Eco47I GGWCC 2 cut(s) 224, 298
Eco88I CYCGRG 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 452
EcoT14I CCWWGG 2 cut(s) 134, 294
ErhI CCWWGG 2 cut(s) 134, 294
FaeI CATG 2 cut(s) 138, 298
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FaqI GGGAC 2 cut(s) 210, 302
FatI CATG 2 cut(s) 134, 294
FauNDI CATATG 1 cut(s) 351
FokI GGATG 1 cut(s) 368
FspBI CTAG 1 cut(s) 417
GsuI CTGGAG 1 cut(s) 51
HaeIII GGCC 1 cut(s) 248
HapII CCGG 2 cut(s) 144, 180
Hin1II CATG 2 cut(s) 138, 298
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 2 cut(s) 73, 290
HpaII CCGG 2 cut(s) 144, 180
Hpy188I TCNGA 2 cut(s) 11, 262
Hpy188III TCNNGA 2 cut(s) 68, 472
HpyAV CCTTC 1 cut(s) 291
HpyCH4V TGCA 1 cut(s) 221
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 218, 254
Hsp92II CATG 2 cut(s) 138, 298
Kzo9I GATC 5 cut(s) 62, 304, 332, 346, 457
LpnPI CCDG 8 cut(s) 50, 81, 106, 118, 157, 193, 207, 250
LweI GCATC 1 cut(s) 266
MaeI CTAG 1 cut(s) 417
MaeIII GTNAC 2 cut(s) 39, 107
MalI GATC 5 cut(s) 64, 306, 334, 348, 459
MboI GATC 5 cut(s) 62, 304, 332, 346, 457
MboII GAAGA 4 cut(s) 11, 24, 263, 454
MflI RGATCY 2 cut(s) 62, 304
MluCI AATT 1 cut(s) 452
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 1 cut(s) 249
MnlI CCTC 3 cut(s) 21, 49, 442
MslI CAYNNNNRTG 2 cut(s) 93, 354
MspI CCGG 2 cut(s) 144, 180
MspR9I CCNGG 1 cut(s) 144
MwoI GCNNNNNNNGC 3 cut(s) 209, 218, 254
NciI CCSGG 1 cut(s) 144
NcoI CCATGG 2 cut(s) 134, 294
NdeI CATATG 1 cut(s) 351
NdeII GATC 5 cut(s) 62, 304, 332, 346, 457
NlaIII CATG 2 cut(s) 138, 298
NlaIV GGNNCC 1 cut(s) 226
PfeI GAWTC 1 cut(s) 73
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
PpuMI RGGWCCY 1 cut(s) 224
Psp5II RGGWCCY 1 cut(s) 224
PspN4I GGNNCC 1 cut(s) 226
PspPI GGNCC 2 cut(s) 224, 298
PspPPI RGGWCCY 1 cut(s) 224
PsuI RGATCY 2 cut(s) 62, 304
RseI CAYNNNNRTG 2 cut(s) 93, 354
Sau3AI GATC 5 cut(s) 62, 304, 332, 346, 457
Sau96I GGNCC 2 cut(s) 224, 298
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 144
SetI ASST 6 cut(s) 210, 226, 269, 283, 370, 418
SfaNI GCATC 1 cut(s) 266
SfcI CTRYAG 1 cut(s) 23
SinI GGWCC 2 cut(s) 224, 298
SmiMI CAYNNNNRTG 2 cut(s) 93, 354
Sse9I AATT 1 cut(s) 452
SsiI CCGC 2 cut(s) 193, 479
SspMI CTAG 1 cut(s) 417
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 2 cut(s) 134, 294
TaqI TCGA 2 cut(s) 101, 456
TasI AATT 1 cut(s) 452
TfiI GAWTC 1 cut(s) 73
TscAI CASTG 1 cut(s) 100
TspDTI ATGAA 3 cut(s) 303, 396, 411
TspRI CASTG 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 224, 298
XapI RAATTY 1 cut(s) 452
XspI CTAG 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.