pycom461g00290

BEST Arabidopsis thaliana protein match is Mitovirus RNA-dependent RNA polymerase (TAIR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000461
OG0029499
Physical Location & Seq
Forward (+)
227040 .. 227997
958 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
ATGGAGAATAGGGTCGCTATGCCGAAGAAAAGAGAGAGGTTGCACCAAGAAATGGAGAAGATAAGGTCATACCAAGGTTTTCCAGATGGGTTAGTAGTTAATTGCTATCACCTTGGTATGGTGCGGTATTTTCTTTTGGAGTCGTGTCGTCCAGATGACAAGTATTTGTTGTCTGTGCGGGATGATTTGGAGCGTAAGCTCACTGAAGAAGGGGACTTCTACGTGGAGAGAGTCATGCTCAGATATTGGGTGGAATCTCTCGTACGATATGTCCGGTGGTATGAGGAAGCCTGTTGTGATTTCACTTTACCTTTGGAACAACTATTAGATCCACCTGCGGTTTGTCTACGTCCTGATCGGAAAGACCTTTTAAGTAAGTTTCACAAATATGGTCTGATGCTCAGATGTTATGACTTAATCCGTCAGCGTCGCTGTCCGCTACCTTTAATGGGCAGCGCTGTACGATTGAATGTTGAAGTTGTGCAATCTCGTCTTTGGTTAAGGGCCAACTTTTGGTTGGAGTTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mitovir_RNA_pol

Protein Analysis

177

Amino Acids

21.3

Weight (kDa)

8.45

Isoelectric Point (pI)

56.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029499)

Species Orthologous Gene IDs
pyrus_communis pycom461g00290
rosa_roxburghii Rroxscaffold_2G00119500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 343
Acc36I ACCTGC 1 cut(s) 343
AccB7I CCANNNNNTGG 2 cut(s) 52, 513
AccI GTMKAC 1 cut(s) 346
AciI CCGC 4 cut(s) 124, 178, 338, 437
AclWI GGATC 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 2 cut(s) 264, 462
AfeI AGCGCT 1 cut(s) 457
AfiI CCNNNNNNNGG 4 cut(s) 52, 118, 449, 513
AgsI TTSAA 2 cut(s) 469, 476
AluBI AGCT 1 cut(s) 199
AluI AGCT 1 cut(s) 199
AlwI GGATC 1 cut(s) 323
Aor51HI AGCGCT 1 cut(s) 457
AoxI GGCC 1 cut(s) 504
ApeKI GCWGC 1 cut(s) 453
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 1 cut(s) 504
AsuHPI GGTGA 1 cut(s) 101
BbvI GCAGC 1 cut(s) 465
BccI CCATC 1 cut(s) 80
BfoI RGCGCY 1 cut(s) 459
BfuAI ACCTGC 1 cut(s) 343
BisI GCNGC 1 cut(s) 454
BlsI GCNGC 1 cut(s) 455
BmgT120I GGNCC 1 cut(s) 504
BmsI GCATC 1 cut(s) 387
BplI GAGNNNNNCTC 2 cut(s) 222, 254
BsaAI YACGTR 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 73, 112
BsaWI WCCGGW 1 cut(s) 273
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 118, 449, 513
BseDI CCNNGG 2 cut(s) 73, 112
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 4 cut(s) 52, 118, 449, 513
BseMII CTCAG 2 cut(s) 253, 415
BseXI GCAGC 1 cut(s) 465
BshFI GGCC 1 cut(s) 506
BsiSI CCGG 1 cut(s) 274
BsiWI CGTACG 1 cut(s) 262
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 4 cut(s) 52, 118, 449, 513
BsmFI GGGAC 1 cut(s) 227
BsnI GGCC 1 cut(s) 506
Bsp143I GATC 2 cut(s) 328, 355
BspACI CCGC 4 cut(s) 124, 178, 338, 437
BspANI GGCC 1 cut(s) 506
BspCNI CTCAG 2 cut(s) 252, 414
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 1 cut(s) 323
BssECI CCNNGG 2 cut(s) 73, 112
BssMI GATC 2 cut(s) 328, 355
BssT1I CCWWGG 2 cut(s) 73, 112
BstBAI YACGTR 1 cut(s) 223
BstDEI CTNAG 2 cut(s) 239, 401
BstF5I GGATG 1 cut(s) 187
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 2 cut(s) 331, 358
BstMBI GATC 2 cut(s) 328, 355
BstV1I GCAGC 1 cut(s) 465
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuRI GGCC 1 cut(s) 506
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 201
BveI ACCTGC 1 cut(s) 343
CfoI GCGC 1 cut(s) 458
Cfr13I GGNCC 1 cut(s) 504
CseI GACGC 1 cut(s) 416
Csp6I GTAC 2 cut(s) 263, 461
CviAII CATG 1 cut(s) 235
CviJI RGCY 3 cut(s) 199, 290, 506
CviKI_1 RGCY 3 cut(s) 199, 290, 506
CviQI GTAC 2 cut(s) 263, 461
DdeI CTNAG 2 cut(s) 239, 401
DpnI GATC 2 cut(s) 330, 357
DpnII GATC 2 cut(s) 328, 355
Eco130I CCWWGG 2 cut(s) 73, 112
Eco47III AGCGCT 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 225
EcoT14I CCWWGG 2 cut(s) 73, 112
ErhI CCWWGG 2 cut(s) 73, 112
FaeI CATG 1 cut(s) 238
FaiI YATR 8 cut(s) 20, 70, 119, 236, 270, 282, 390, 411
FaqI GGGAC 1 cut(s) 227
FatI CATG 1 cut(s) 234
FauI CCCGC 1 cut(s) 171
FblI GTMKAC 1 cut(s) 346
Fnu4HI GCNGC 1 cut(s) 454
FokI GGATG 1 cut(s) 194
Fsp4HI GCNGC 1 cut(s) 454
GlaI GCGC 1 cut(s) 457
GluI GCNGC 1 cut(s) 454
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 1 cut(s) 506
HapII CCGG 1 cut(s) 274
HgaI GACGC 1 cut(s) 416
HhaI GCGC 1 cut(s) 458
Hin1II CATG 1 cut(s) 238
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HinfI GANTC 3 cut(s) 140, 231, 254
HpaII CCGG 1 cut(s) 274
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 1 cut(s) 347
Hpy188I TCNGA 4 cut(s) 242, 360, 396, 404
Hpy188III TCNNGA 3 cut(s) 83, 152, 353
Hpy8I GTNNAC 1 cut(s) 347
Hpy99I CGWCG 1 cut(s) 432
HpyAV CCTTC 1 cut(s) 203
HpyCH4IV ACGT 2 cut(s) 222, 349
HpyCH4V TGCA 2 cut(s) 43, 484
HpyF3I CTNAG 2 cut(s) 239, 401
HpySE526I ACGT 2 cut(s) 222, 349
Hsp92II CATG 1 cut(s) 238
HspAI GCGC 1 cut(s) 456
Kzo9I GATC 2 cut(s) 328, 355
LmnI GCTCC 1 cut(s) 190
LpnPI CCDG 6 cut(s) 96, 165, 287, 304, 348, 366
Lsp1109I GCAGC 1 cut(s) 465
LweI GCATC 1 cut(s) 387
MaeII ACGT 2 cut(s) 222, 349
MalI GATC 2 cut(s) 330, 357
MboI GATC 2 cut(s) 328, 355
MboII GAAGA 3 cut(s) 37, 70, 218
MflI RGATCY 1 cut(s) 328
MluCI AATT 1 cut(s) 100
MlyI GAGTC 2 cut(s) 149, 240
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 2 cut(s) 30, 277
MseI TTAA 6 cut(s) 99, 371, 416, 446, 500, 529
MslI CAYNNNNRTG 1 cut(s) 387
MspI CCGG 1 cut(s) 274
NdeII GATC 2 cut(s) 328, 355
NlaIII CATG 1 cut(s) 238
PaqCI CACCTGC 1 cut(s) 343
PfeI GAWTC 1 cut(s) 254
Pfl23II CGTACG 1 cut(s) 262
PflMI CCANNNNNTGG 2 cut(s) 52, 513
PkrI GCNGC 1 cut(s) 455
PleI GAGTC 2 cut(s) 148, 239
PpsI GAGTC 2 cut(s) 148, 239
Ppu21I YACGTR 1 cut(s) 223
PspLI CGTACG 1 cut(s) 262
PspPI GGNCC 1 cut(s) 504
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 2 cut(s) 264, 462
RsaNI GTAC 2 cut(s) 263, 461
RseI CAYNNNNRTG 1 cut(s) 387
SaqAI TTAA 6 cut(s) 99, 371, 416, 446, 500, 529
SatI GCNGC 1 cut(s) 454
Sau3AI GATC 2 cut(s) 328, 355
Sau96I GGNCC 1 cut(s) 504
SchI GAGTC 2 cut(s) 149, 240
SfaNI GCATC 1 cut(s) 387
SmiMI CAYNNNNRTG 1 cut(s) 387
Sse9I AATT 1 cut(s) 100
SsiI CCGC 4 cut(s) 124, 178, 338, 437
StyI CCWWGG 2 cut(s) 73, 112
TaiI ACGT 2 cut(s) 225, 352
TasI AATT 1 cut(s) 100
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 6 cut(s) 99, 371, 416, 446, 500, 529
Tru9I TTAA 6 cut(s) 99, 371, 416, 446, 500, 529
TscAI CASTG 1 cut(s) 208
TseI GCWGC 1 cut(s) 453
TspGWI ACGGA 1 cut(s) 410
TspRI CASTG 1 cut(s) 208
Van91I CCANNNNNTGG 2 cut(s) 52, 513
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.