RCHIOBHM_MTG0499151

NADH dehydrogenase (quinone) activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Mt
OG0030547
Physical Location & Seq
Reverse (-)
270091 .. 271400
1310 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 753 bp
ATGAGATTCTTGCCGAATCCGAGTGCTTCTGTTGCGTATAATGTAAATCCCGTAGCGGATCAATTCCAACGAGCCTTTCAAACTAGTACTTTTTGTAATCGACTCTATAGCTTCTTCAATAAACGCTGGTTCTTCGATCAAGTTTTGAATGACTTTCTAGTCAGATCGTTCCTGCGTTTCGGATATGAAGTCTCATTCGAAGCTTTAGACAAAGGTGCTATTGAGATATTGGGCCCTTATGGTATCTCGTACACATTCCGACGATTGGCCGAGCGAATAAGTCAACTTCAAAGTGGATTTGTTGTGCGAAGAGTGCGTTATGACCTGTGGCCGCCTGCCTGGTGGGGGCGGCTCCTCCGTTGTGGCTCGACTATAAAAGGAGAGGGGGTTGAGAAGAGCTTGCTGTCTACTTCGCGAGCCTTCACTGTCCCGTTATGTAACTTCCCTTCTTTCGTCCGTCCACGAGGCTGTAAAAAGATAAAAAGGGGCGGCTATCCAGCAGGAGTCGTTTTGGTTGTATGCCACGAGGTCCCTATGGACAAGGGGACAAGTGAATCATCGCTTTTGGGCGCGGGCAGCCCTCTACCATCCATCCCATTGCATTCCAACGTCTCGTATTTAATGATTTCAGCAAGAGCAGCCGGACGGACTACTATAGTGAGTCTAGTGACTACTAGAGTAGAGTTGAGTCAAAAGGTATGGTATAGCAGCCTTTCCGAGCGAGCCTCTGGGATCTCCTGTAAACCCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

28.03

Weight (kDa)

9.99

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADH5_C PF06455 31 - 100 3.4e-07 NADH dehydrogenase subunit 5 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030547)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_MTg0499151
rosa_samantha Rh3BG350000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 407
AccII CGCG 2 cut(s) 415, 572
AciI CCGC 5 cut(s) 56, 332, 349, 489, 572
AclWI GGATC 2 cut(s) 66, 740
AcoI YGGCCR 2 cut(s) 267, 329
AfaI GTAC 2 cut(s) 88, 251
AfiI CCNNNNNNNGG 2 cut(s) 265, 345
AgsI TTSAA 4 cut(s) 80, 118, 148, 290
AhlI ACTAGT 1 cut(s) 83
AjnI CCWGG 1 cut(s) 338
AjuI GAANNNNNNNTTGG 2 cut(s) 60, 92
AluBI AGCT 3 cut(s) 111, 203, 399
AluI AGCT 3 cut(s) 111, 203, 399
Alw26I GTCTC 2 cut(s) 196, 616
AlwI GGATC 2 cut(s) 66, 740
AoxI GGCC 3 cut(s) 232, 267, 329
ApaI GGGCCC 1 cut(s) 236
ApeKI GCWGC 3 cut(s) 576, 638, 708
AspLEI GCGC 1 cut(s) 572
AspS9I GGNCC 3 cut(s) 232, 233, 529
AsuII TTCGAA 1 cut(s) 198
AvaII GGWCC 1 cut(s) 529
BaeGI GKGCMC 1 cut(s) 236
BanII GRGCYC 1 cut(s) 236
BarI GAAGNNNNNNTAC 2 cut(s) 430, 462
BauI CACGAG 2 cut(s) 462, 524
BbvI GCAGC 3 cut(s) 588, 650, 720
BccI CCATC 2 cut(s) 595, 599
BciT130I CCWGG 1 cut(s) 340
BcoDI GTCTC 2 cut(s) 196, 616
BcuI ACTAGT 1 cut(s) 83
BfaI CTAG 4 cut(s) 84, 158, 665, 675
BfmI CTRYAG 2 cut(s) 106, 654
BisI GCNGC 6 cut(s) 332, 350, 490, 577, 639, 709
BlsI GCNGC 6 cut(s) 333, 351, 491, 578, 640, 710
BmcAI AGTACT 1 cut(s) 88
Bme1390I CCNGG 1 cut(s) 340
Bme18I GGWCC 1 cut(s) 529
BmgT120I GGNCC 3 cut(s) 232, 233, 529
BmiI GGNNCC 3 cut(s) 234, 353, 531
BmrFI CCNGG 1 cut(s) 340
BplI GAGNNNNNCTC 2 cut(s) 710, 742
Bpu14I TTCGAA 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 345
Bse3DI GCAATG 1 cut(s) 596
BseBI CCWGG 1 cut(s) 340
BseGI GGATG 2 cut(s) 587, 591
BseLI CCNNNNNNNGG 2 cut(s) 265, 345
BseMI GCAATG 1 cut(s) 596
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 236
BseXI GCAGC 3 cut(s) 588, 650, 720
Bsh1236I CGCG 2 cut(s) 415, 572
BshFI GGCC 3 cut(s) 234, 269, 331
BsiSI CCGG 1 cut(s) 642
BslFI GGGAC 3 cut(s) 413, 515, 559
BslI CCNNNNNNNGG 2 cut(s) 265, 345
BsmAI GTCTC 2 cut(s) 196, 616
BsmBI CGTCTC 1 cut(s) 616
BsmFI GGGAC 3 cut(s) 413, 515, 559
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 3 cut(s) 234, 269, 331
Bsp119I TTCGAA 1 cut(s) 198
Bsp120I GGGCCC 1 cut(s) 232
Bsp1286I GDGCHC 1 cut(s) 236
Bsp143I GATC 4 cut(s) 58, 136, 164, 732
Bsp68I TCGCGA 1 cut(s) 415
BspACI CCGC 5 cut(s) 56, 332, 349, 489, 572
BspANI GGCC 3 cut(s) 234, 269, 331
BspFNI CGCG 2 cut(s) 415, 572
BspLI GGNNCC 3 cut(s) 234, 353, 531
BspPI GGATC 2 cut(s) 66, 740
BspQI GCTCTTC 1 cut(s) 389
BspT104I TTCGAA 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 596
BssMI GATC 4 cut(s) 58, 136, 164, 732
BssSI CACGAG 2 cut(s) 462, 524
Bst2BI CACGAG 2 cut(s) 462, 524
Bst2UI CCWGG 1 cut(s) 340
Bst4CI ACNGT 1 cut(s) 427
Bst6I CTCTTC 2 cut(s) 304, 389
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 5 cut(s) 336, 401, 417, 574, 723
BstF5I GGATG 2 cut(s) 587, 591
BstFNI CGCG 2 cut(s) 415, 572
BstHHI GCGC 1 cut(s) 572
BstKTI GATC 4 cut(s) 61, 139, 167, 735
BstMAI GTCTC 2 cut(s) 196, 616
BstMBI GATC 4 cut(s) 58, 136, 164, 732
BstMWI GCNNNNNNNGC 4 cut(s) 32, 313, 576, 638
BstNI CCWGG 1 cut(s) 340
BstSCI CCNGG 1 cut(s) 338
BstSFI CTRYAG 2 cut(s) 106, 654
BstSLI GKGCMC 1 cut(s) 236
BstUI CGCG 2 cut(s) 415, 572
BstV1I GCAGC 3 cut(s) 588, 650, 720
BstX2I RGATCY 1 cut(s) 732
BstYI RGATCY 1 cut(s) 732
BsuRI GGCC 3 cut(s) 234, 269, 331
BtgZI GCGATG 1 cut(s) 543
BtsCI GGATG 2 cut(s) 587, 591
BtsIMutI CAGTG 1 cut(s) 423
BtuMI TCGCGA 1 cut(s) 415
Cac8I GCNNGC 5 cut(s) 336, 401, 417, 574, 723
CfoI GCGC 1 cut(s) 572
Cfr13I GGNCC 3 cut(s) 232, 233, 529
Csp6I GTAC 2 cut(s) 87, 250
CviAII CATG 1 cut(s) 750
CviQI GTAC 2 cut(s) 87, 250
DpnI GATC 4 cut(s) 60, 138, 166, 734
DpnII GATC 4 cut(s) 58, 136, 164, 732
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 2 cut(s) 267, 329
Eam1104I CTCTTC 2 cut(s) 304, 389
EarI CTCTTC 2 cut(s) 304, 389
Eco24I GRGCYC 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 529
EcoO109I RGGNCCY 2 cut(s) 233, 529
EcoRII CCWGG 1 cut(s) 338
EcoT38I GRGCYC 1 cut(s) 236
Esp3I CGTCTC 1 cut(s) 616
FaeI CATG 1 cut(s) 753
FaqI GGGAC 3 cut(s) 413, 515, 559
FatI CATG 1 cut(s) 749
FauI CCCGC 1 cut(s) 565
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 6 cut(s) 332, 350, 490, 577, 639, 709
FokI GGATG 2 cut(s) 574, 578
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 6 cut(s) 332, 350, 490, 577, 639, 709
FspBI CTAG 4 cut(s) 84, 158, 665, 675
GlaI GCGC 1 cut(s) 571
GluI GCNGC 6 cut(s) 332, 350, 490, 577, 639, 709
HaeIII GGCC 3 cut(s) 234, 269, 331
HapII CCGG 1 cut(s) 642
HhaI GCGC 1 cut(s) 572
Hin1II CATG 1 cut(s) 753
Hin6I GCGC 1 cut(s) 570
HinP1I GCGC 1 cut(s) 570
HincII GTYRAC 1 cut(s) 284
HindII GTYRAC 1 cut(s) 284
HindIII AAGCTT 1 cut(s) 201
HinfI GANTC 7 cut(s) 6, 16, 102, 504, 554, 661, 688
HpaII CCGG 1 cut(s) 642
Hpy166II GTNNAC 5 cut(s) 252, 284, 408, 461, 743
Hpy188I TCNGA 5 cut(s) 21, 164, 182, 260, 718
Hpy188III TCNNGA 1 cut(s) 414
Hpy8I GTNNAC 5 cut(s) 252, 284, 408, 461, 743
Hpy99I CGWCG 1 cut(s) 264
HpyAV CCTTC 2 cut(s) 430, 456
HpyCH4III ACNGT 1 cut(s) 427
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 1 cut(s) 601
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 313, 576, 638
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 1 cut(s) 753
HspAI GCGC 1 cut(s) 570
Kzo9I GATC 4 cut(s) 58, 136, 164, 732
LguI GCTCTTC 1 cut(s) 389
LmnI GCTCC 1 cut(s) 357
Lsp1109I GCAGC 3 cut(s) 588, 650, 720
MaeI CTAG 4 cut(s) 84, 158, 665, 675
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 2 cut(s) 437, 667
MalI GATC 4 cut(s) 60, 138, 166, 734
MboI GATC 4 cut(s) 58, 136, 164, 732
MboII GAAGA 4 cut(s) 106, 124, 321, 406
MflI RGATCY 1 cut(s) 732
MhlI GDGCHC 1 cut(s) 236
MluCI AATT 1 cut(s) 62
MlyI GAGTC 4 cut(s) 96, 513, 670, 697
MmeI TCCRAC 3 cut(s) 91, 283, 630
MnlI CCTC 6 cut(s) 365, 376, 458, 520, 591, 736
MseI TTAA 1 cut(s) 620
MspI CCGG 1 cut(s) 642
MspR9I CCNGG 1 cut(s) 340
Mva1269I GAATGC 1 cut(s) 601
MvaI CCWGG 1 cut(s) 340
MvnI CGCG 2 cut(s) 415, 572
MwoI GCNNNNNNNGC 4 cut(s) 32, 313, 576, 638
NdeII GATC 4 cut(s) 58, 136, 164, 732
NlaIII CATG 1 cut(s) 753
NlaIV GGNNCC 3 cut(s) 234, 353, 531
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 1 cut(s) 667
NruI TCGCGA 1 cut(s) 415
NspV TTCGAA 1 cut(s) 198
PciSI GCTCTTC 1 cut(s) 389
PctI GAATGC 1 cut(s) 601
PfeI GAWTC 3 cut(s) 6, 16, 554
PkrI GCNGC 6 cut(s) 333, 351, 491, 578, 640, 710
PleI GAGTC 4 cut(s) 96, 512, 669, 696
PpsI GAGTC 4 cut(s) 96, 512, 669, 696
PpuMI RGGWCCY 1 cut(s) 529
Psp5II RGGWCCY 1 cut(s) 529
Psp6I CCWGG 1 cut(s) 338
PspGI CCWGG 1 cut(s) 338
PspN4I GGNNCC 3 cut(s) 234, 353, 531
PspOMI GGGCCC 1 cut(s) 232
PspPI GGNCC 3 cut(s) 232, 233, 529
PspPPI RGGWCCY 1 cut(s) 529
PsuI RGATCY 1 cut(s) 732
RruI TCGCGA 1 cut(s) 415
RsaI GTAC 2 cut(s) 88, 251
RsaNI GTAC 2 cut(s) 87, 250
SapI GCTCTTC 1 cut(s) 389
SaqAI TTAA 1 cut(s) 620
SatI GCNGC 6 cut(s) 332, 350, 490, 577, 639, 709
Sau3AI GATC 4 cut(s) 58, 136, 164, 732
Sau96I GGNCC 3 cut(s) 232, 233, 529
ScaI AGTACT 1 cut(s) 88
SchI GAGTC 4 cut(s) 96, 513, 670, 697
ScrFI CCNGG 1 cut(s) 340
SduI GDGCHC 1 cut(s) 236
SetI ASST 8 cut(s) 113, 205, 217, 327, 401, 531, 612, 699
SfcI CTRYAG 2 cut(s) 106, 654
SfuI TTCGAA 1 cut(s) 198
SinI GGWCC 1 cut(s) 529
SpeI ACTAGT 1 cut(s) 83
Sse9I AATT 1 cut(s) 62
SsiI CCGC 5 cut(s) 56, 332, 349, 489, 572
SspMI CTAG 4 cut(s) 84, 158, 665, 675
StyD4I CCNGG 1 cut(s) 338
TaaI ACNGT 1 cut(s) 427
TaiI ACGT 1 cut(s) 612
TaqI TCGA 4 cut(s) 100, 135, 198, 368
TasI AATT 1 cut(s) 62
TatI WGTACW 1 cut(s) 86
TauI GCSGC 3 cut(s) 334, 352, 492
TfiI GAWTC 3 cut(s) 6, 16, 554
Tru1I TTAA 1 cut(s) 620
Tru9I TTAA 1 cut(s) 620
TscAI CASTG 1 cut(s) 430
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 3 cut(s) 576, 638, 708
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 1 cut(s) 201
TspGWI ACGGA 3 cut(s) 347, 446, 661
TspRI CASTG 1 cut(s) 430
VpaK11BI GGWCC 1 cut(s) 529
XmiI GTMKAC 1 cut(s) 407
XspI CTAG 4 cut(s) 84, 158, 665, 675
ZrmI AGTACT 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.