RchiOBHm_Chr7g0233231

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0030471
Physical Location & Seq
Forward (+)
57668041 .. 57669430
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 918 bp
ATGCTCGGTGTGCTTTCGTATAATGCGATTTTAGATGCAGTGATTAGGTCTAGAGGGTCTGTTCAGTTTGCTGAGGAGGTGTTTAGTGAGATGATTAGAAATGGGGTTTCGCCGAATGTGTATACATATAACATTTTGATTAGGGGTTTCAGCGCGGCGGGGAATTTGGAAATGGTGCTGAATTTTTGGAGTGAAATGGGTAGGATGGATGATGCGTTCGGATTGTTGAGATCGATGGCATTGAAGGGTTTGGAGCCTAATTTGATTTCGTATAATGTGGTGATTCATGGGTTGTGTCGAGAAGGGAGGATGAGTGAGACTAGTCAGGTTGTTGAGGAGATGAAAAGAAAGGGTTTTGTTCCTGATGAGGTGACGAATAATACACTGATTAGTGGGTATTGCAAGCAAGGTAATTTTCACCAAGCACTTGTGTTGCAAGAGGAGATGCGGAGGAATGGCTTGTCTCCGAATGTTGTCACTTATACAGCGTTGATCAATGCCATGTGTAAGGCTAAGAATTTGAATCGATCAATGGAGTTTCGTGTTAGAGGACTTCGACCAAATGAGAGGACGTATACTACATTGATAGATGGCTTTGCCCAACAAGGGTTCTTAAATGAAGCTGACGGTCTTCTGAATGAAATGGTTGGGGGTGGATTTTCTCCTTCAGTTGTGACTTACAGTGCCCTTATCAATGGATACTGCTTATTAGGCAGGATGGAAGAGGCAATGGGAATTGTACAAGATATGGTAGGGAAAGGGTTATCCCCTGATGTAGTAAGTTATAGTACTATTATCACTGGGTTTTGTCGGCATCAAGAACTGGAGAGTGCATTCCAAATGAAGATGGAGATGGTGGAGAAGGGTATATCACCCGATGCTGTTACCTATTCATCACTAATTCAAGGTGTCCGTTAG

Protein Analysis

305

Amino Acids

33.83

Weight (kDa)

5.57

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 4 - 48 9.3e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 4 - 50 1.7e-13 PPR repeat family
PPR_long PF17177 20 - 128 3.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 34 - 57 2e-06 PPR repeat
PPR_2 PF13041 59 - 100 8.1e-09 PPR repeat family
PPR_1 PF12854 82 - 115 2.1e-11 PPR repeat
PPR_2 PF13041 86 - 125 2e-11 PPR repeat family
PPR PF01535 89 - 119 2.6e-07 PPR repeat
PPR_long PF17177 106 - 180 3.5e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 110 - 168 2e-14 Pentatricopeptide repeat domain
PPR_1 PF12854 117 - 150 3.7e-10 PPR repeat
PPR_2 PF13041 121 - 170 5.3e-19 PPR repeat family
PPR PF01535 127 - 154 1.3e-06 PPR repeat
PPR_1 PF12854 152 - 180 1.6e-08 PPR repeat
PPR_3 PF13812 181 - 234 2.3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 183 - 216 1.8e-09 PPR repeat
PPR_2 PF13041 187 - 235 2.4e-14 PPR repeat family
PPR PF01535 191 - 219 8.1e-07 PPR repeat
PPR_long PF17177 213 - 301 6e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 219 - 251 1.3e-10 PPR repeat
PPR_2 PF13041 222 - 271 1.2e-16 PPR repeat family
PPR PF01535 225 - 255 1.6e-08 PPR repeat
PPR_3 PF13812 246 - 302 1.2e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 253 - 286 1.5e-10 PPR repeat
PPR_2 PF13041 257 - 303 1.4e-16 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0030471)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0233231
rosa_multiflora Rmu_sc0008314.1_g000011

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 122, 575
AccII CGCG 1 cut(s) 155
AciI CCGC 3 cut(s) 155, 158, 448
AcsI RAATTY 3 cut(s) 163, 181, 517
AcuI CTGAAG 1 cut(s) 651
AfaI GTAC 2 cut(s) 741, 790
AfiI CCNNNNNNNGG 1 cut(s) 606
AgsI TTSAA 3 cut(s) 244, 523, 905
AhlI ACTAGT 1 cut(s) 320
AloI GAACNNNNNNTCC 2 cut(s) 200, 232
AluBI AGCT 1 cut(s) 623
AluI AGCT 1 cut(s) 623
Alw26I GTCTC 2 cut(s) 311, 468
ApoI RAATTY 3 cut(s) 163, 181, 517
AspLEI GCGC 1 cut(s) 155
AsuHPI GGTGA 4 cut(s) 292, 382, 410, 864
BaeGI GKGCMC 1 cut(s) 688
BbsI GAAGAC 1 cut(s) 623
BbvCI CCTCAGC 1 cut(s) 72
BccI CCATC 6 cut(s) 199, 229, 584, 712, 841, 847
BciVI GTATCC 1 cut(s) 692
BclI TGATCA 1 cut(s) 492
BcoDI GTCTC 2 cut(s) 311, 468
BcuI ACTAGT 1 cut(s) 320
BfaI CTAG 2 cut(s) 51, 321
BfuI GTATCC 1 cut(s) 692
BisI GCNGC 1 cut(s) 156
BlsI GCNGC 1 cut(s) 157
BmcAI AGTACT 1 cut(s) 790
BmiI GGNNCC 1 cut(s) 255
BmrI ACTGGG 1 cut(s) 810
BmsI GCATC 5 cut(s) 25, 202, 435, 823, 868
BmuI ACTGGG 1 cut(s) 810
BpiI GAAGAC 1 cut(s) 623
BpmI CTGGAG 1 cut(s) 845
Bpu10I CCTNAGC 1 cut(s) 72
Bsa29I ATCGAT 2 cut(s) 233, 526
BsaXI ACNNNNNCTCC 2 cut(s) 298, 328
Bsc4I CCNNNNNNNGG 1 cut(s) 606
Bse1I ACTGG 2 cut(s) 805, 828
Bse3DI GCAATG 1 cut(s) 735
BseCI ATCGAT 2 cut(s) 233, 526
BseGI GGATG 4 cut(s) 210, 214, 315, 723
BseLI CCNNNNNNNGG 1 cut(s) 606
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 2 cut(s) 805, 828
BseRI GAGGAG 3 cut(s) 89, 350, 455
BseSI GKGCMC 1 cut(s) 688
Bsh1236I CGCG 1 cut(s) 155
BshVI ATCGAT 2 cut(s) 233, 526
BslI CCNNNNNNNGG 1 cut(s) 606
BsmAI GTCTC 2 cut(s) 311, 468
BsmI GAATGC 1 cut(s) 833
Bsp1286I GDGCHC 1 cut(s) 688
Bsp1407I TGTACA 1 cut(s) 739
Bsp143I GATC 3 cut(s) 230, 492, 527
BspACI CCGC 3 cut(s) 155, 158, 448
BspCNI CTCAG 1 cut(s) 64
BspDI ATCGAT 2 cut(s) 233, 526
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 735
BsrGI TGTACA 1 cut(s) 739
BsrI ACTGG 2 cut(s) 805, 828
BssMI GATC 3 cut(s) 230, 492, 527
BssNAI GTATAC 2 cut(s) 123, 576
Bst1107I GTATAC 2 cut(s) 123, 576
Bst4CI ACNGT 2 cut(s) 629, 683
Bst6I CTCTTC 1 cut(s) 717
BstAUI TGTACA 1 cut(s) 739
BstC8I GCNNGC 1 cut(s) 404
BstDEI CTNAG 2 cut(s) 72, 513
BstF5I GGATG 4 cut(s) 210, 214, 315, 723
BstFNI CGCG 1 cut(s) 155
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 3 cut(s) 233, 495, 530
BstMAI GTCTC 2 cut(s) 311, 468
BstMBI GATC 3 cut(s) 230, 492, 527
BstMWI GCNNNNNNNGC 2 cut(s) 10, 711
BstSLI GKGCMC 1 cut(s) 688
BstUI CGCG 1 cut(s) 155
BstV2I GAAGAC 1 cut(s) 623
BstZ17I GTATAC 2 cut(s) 123, 576
Bsu15I ATCGAT 2 cut(s) 233, 526
BsuI GTATCC 1 cut(s) 692
BsuTUI ATCGAT 2 cut(s) 233, 526
BtsCI GGATG 4 cut(s) 210, 214, 315, 723
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 4 cut(s) 45, 383, 688, 798
Cac8I GCNNGC 1 cut(s) 404
CfoI GCGC 1 cut(s) 155
ClaI ATCGAT 2 cut(s) 233, 526
Csp6I GTAC 2 cut(s) 740, 789
CviAII CATG 2 cut(s) 287, 502
CviJI RGCY 5 cut(s) 256, 459, 512, 594, 623
CviKI_1 RGCY 5 cut(s) 256, 459, 512, 594, 623
CviQI GTAC 2 cut(s) 740, 789
DdeI CTNAG 2 cut(s) 72, 513
DpnI GATC 3 cut(s) 232, 494, 529
DpnII GATC 3 cut(s) 230, 492, 527
Eam1104I CTCTTC 1 cut(s) 717
EarI CTCTTC 1 cut(s) 717
Eco57I CTGAAG 1 cut(s) 651
FaeI CATG 2 cut(s) 290, 505
FatI CATG 2 cut(s) 286, 501
FauI CCCGC 1 cut(s) 151
FbaI TGATCA 1 cut(s) 492
FblI GTMKAC 2 cut(s) 122, 575
Fnu4HI GCNGC 1 cut(s) 156
FokI GGATG 4 cut(s) 217, 221, 322, 730
Fsp4HI GCNGC 1 cut(s) 156
FspBI CTAG 2 cut(s) 51, 321
GlaI GCGC 1 cut(s) 154
GluI GCNGC 1 cut(s) 156
GsuI CTGGAG 1 cut(s) 845
HhaI GCGC 1 cut(s) 155
Hin1II CATG 2 cut(s) 290, 505
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HinfI GANTC 2 cut(s) 283, 523
HphI GGTGA 4 cut(s) 292, 382, 410, 864
Hpy166II GTNNAC 2 cut(s) 123, 576
Hpy188I TCNGA 3 cut(s) 221, 468, 636
Hpy188III TCNNGA 4 cut(s) 51, 299, 362, 818
Hpy8I GTNNAC 2 cut(s) 123, 576
HpyAV CCTTC 4 cut(s) 238, 296, 675, 856
HpyCH4III ACNGT 2 cut(s) 629, 683
HpyCH4IV ACGT 1 cut(s) 572
HpyCH4V TGCA 4 cut(s) 38, 402, 436, 833
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 711
HpyF3I CTNAG 2 cut(s) 72, 513
HpySE526I ACGT 1 cut(s) 572
Hsp92II CATG 2 cut(s) 290, 505
HspAI GCGC 1 cut(s) 153
Ksp22I TGATCA 1 cut(s) 492
Kzo9I GATC 3 cut(s) 230, 492, 527
LmnI GCTCC 1 cut(s) 253
LpnPI CCDG 6 cut(s) 311, 375, 700, 783, 786, 809
LweI GCATC 5 cut(s) 25, 202, 435, 823, 868
MaeI CTAG 2 cut(s) 51, 321
MaeII ACGT 1 cut(s) 572
MaeIII GTNAC 4 cut(s) 370, 475, 673, 883
MalI GATC 3 cut(s) 232, 494, 529
MboI GATC 3 cut(s) 230, 492, 527
MboII GAAGA 3 cut(s) 623, 734, 856
MhlI GDGCHC 1 cut(s) 688
MluCI AATT 7 cut(s) 163, 181, 259, 412, 517, 735, 900
MseI TTAA 1 cut(s) 614
Mva1269I GAATGC 1 cut(s) 833
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 2 cut(s) 10, 711
NdeII GATC 3 cut(s) 230, 492, 527
NlaIII CATG 2 cut(s) 290, 505
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 3 cut(s) 370, 475, 673
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 1 cut(s) 833
PfeI GAWTC 2 cut(s) 283, 523
PkrI GCNGC 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 255
RsaI GTAC 2 cut(s) 741, 790
RsaNI GTAC 2 cut(s) 740, 789
SaqAI TTAA 1 cut(s) 614
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 3 cut(s) 230, 492, 527
ScaI AGTACT 1 cut(s) 790
SduI GDGCHC 1 cut(s) 688
SetI ASST 9 cut(s) 50, 81, 330, 372, 412, 575, 625, 890, 910
SfaNI GCATC 5 cut(s) 25, 202, 435, 823, 868
SpeI ACTAGT 1 cut(s) 320
Sse9I AATT 7 cut(s) 163, 181, 259, 412, 517, 735, 900
SsiI CCGC 3 cut(s) 155, 158, 448
SspMI CTAG 2 cut(s) 51, 321
TaaI ACNGT 2 cut(s) 629, 683
TaiI ACGT 1 cut(s) 575
TaqI TCGA 4 cut(s) 233, 298, 526, 556
TasI AATT 7 cut(s) 163, 181, 259, 412, 517, 735, 900
TatI WGTACW 2 cut(s) 739, 788
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 2 cut(s) 283, 523
Tru1I TTAA 1 cut(s) 614
Tru9I TTAA 1 cut(s) 614
TscAI CASTG 4 cut(s) 45, 390, 688, 805
TseFI GTSAC 3 cut(s) 370, 475, 673
Tsp45I GTSAC 3 cut(s) 370, 475, 673
TspDTI ATGAA 6 cut(s) 275, 356, 633, 654, 857, 882
TspGWI ACGGA 1 cut(s) 902
TspRI CASTG 4 cut(s) 45, 390, 688, 805
XapI RAATTY 3 cut(s) 163, 181, 517
XbaI TCTAGA 1 cut(s) 50
XmiI GTMKAC 2 cut(s) 122, 575
XspI CTAG 2 cut(s) 51, 321
ZrmI AGTACT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.