RchiOBHm_Chr6g0273951

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0021446
Physical Location & Seq
Reverse (-)
34982028 .. 34985179
3152 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
ATGAACTCTCTAACAATGCCAAATTCGATAGATTTCCACACCCCGCGAATTTTGGCTCAAGAGCTCTGTCAGTTGATGCTGTTCAAGTTACTAATGGAGATGCAAGCAAAGGAGGGCCACTTGTTGAGTATGAGCGAAGAATTGTTGCGGGCGAACTTGTTGATGGTGATGCCTGCCAGGAGTAGATGGTTGTGGTCTCGTTTCATCCCACAGTCTTCAAATGCCTCCCCTGTTAAAGGTCTTTATATTTATGGAGGAGTGGGAACTGGAAAAACCATGTTGATGGACTTGTTTTTCGATCAACTACAATGCAATTGGAGGAAAAAGAGGATTCATTTTCATGATTTTATGTTGAATGTCCACAGCTGCTTGCGAAAGCACCAGGATGTAGAAGACCCTCTTGAAGTTGTTGCAGGAGAGATATCTGATGAGGCAGTGACTAATGTTGCTGATGCATTAATACTAAATCGTCTATTTGGACATCTATTCGACAATGGCGTTATTCTTGTTGCCACCTCCAATCGTGCTCCAGATAACCTGTATGAACGTGGATTGCAGAGGGATCTGTTTCTGCCCTTTATTGCTGCTTTGAAGGTTTGTATCTGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

203

Amino Acids

23.31

Weight (kDa)

6.65

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 51 - 198 7.2e-44 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021446)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0273951
rosa_samantha Rh4CG033800 Rh6DG188500
rosa_wichuraiana Rw1G030190 Rw6G012200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 46
AciI CCGC 2 cut(s) 44, 148
AclWI GGATC 1 cut(s) 570
AcsI RAATTY 2 cut(s) 22, 48
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 5 cut(s) 85, 219, 355, 404, 592
AjnI CCWGG 2 cut(s) 176, 381
AluBI AGCT 2 cut(s) 64, 366
AluI AGCT 2 cut(s) 64, 366
Alw21I GWGCWC 2 cut(s) 66, 529
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 1 cut(s) 570
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 2 cut(s) 366, 584
ApoI RAATTY 2 cut(s) 22, 48
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AseI ATTAAT 1 cut(s) 458
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 178
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 2 cut(s) 207, 399
Bbv12I GWGCWC 2 cut(s) 66, 529
BbvI GCAGC 2 cut(s) 353, 571
BccI CCATC 3 cut(s) 157, 180, 277
BciT130I CCWGG 2 cut(s) 178, 383
BcoDI GTCTC 1 cut(s) 201
BisI GCNGC 2 cut(s) 367, 585
BlsI GCNGC 2 cut(s) 368, 586
Bme1390I CCNGG 2 cut(s) 178, 383
BmgT120I GGNCC 1 cut(s) 115
BmrFI CCNGG 2 cut(s) 178, 383
BmsI GCATC 4 cut(s) 66, 90, 159, 442
BpiI GAAGAC 2 cut(s) 207, 399
BpmI CTGGAG 1 cut(s) 513
BpuEI CTTGAG 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 201
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse1I ACTGG 1 cut(s) 271
BseBI CCWGG 2 cut(s) 178, 383
BseGI GGATG 3 cut(s) 204, 391, 612
BseLI CCNNNNNNNGG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 271
BseRI GAGGAG 1 cut(s) 270
BseXI GCAGC 2 cut(s) 353, 571
Bsh1236I CGCG 1 cut(s) 46
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 2 cut(s) 66, 529
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 1 cut(s) 201
BsnI GGCC 1 cut(s) 117
Bso31I GGTCTC 1 cut(s) 201
Bsp1286I GDGCHC 2 cut(s) 66, 529
Bsp143I GATC 2 cut(s) 298, 562
BspACI CCGC 2 cut(s) 44, 148
BspANI GGCC 1 cut(s) 117
BspFNI CGCG 1 cut(s) 46
BspHI TCATGA 1 cut(s) 340
BspPI GGATC 1 cut(s) 570
BspTNI GGTCTC 1 cut(s) 201
BsrI ACTGG 1 cut(s) 271
BssMI GATC 2 cut(s) 298, 562
Bst2UI CCWGG 2 cut(s) 178, 383
Bst4CI ACNGT 1 cut(s) 213
BstC8I GCNNGC 4 cut(s) 105, 150, 174, 371
BstENI CCTNNNNNAGG 1 cut(s) 234
BstF5I GGATG 3 cut(s) 204, 391, 612
BstFNI CGCG 1 cut(s) 46
BstKTI GATC 2 cut(s) 301, 565
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 2 cut(s) 298, 562
BstNI CCWGG 2 cut(s) 178, 383
BstSCI CCNGG 2 cut(s) 176, 381
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 2 cut(s) 353, 571
BstV2I GAAGAC 2 cut(s) 207, 399
BstX2I RGATCY 1 cut(s) 562
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 1 cut(s) 562
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 3 cut(s) 204, 391, 612
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 1 cut(s) 441
Cac8I GCNNGC 4 cut(s) 105, 150, 174, 371
CciI TCATGA 1 cut(s) 340
Cfr13I GGNCC 1 cut(s) 115
CviAII CATG 2 cut(s) 277, 341
CviJI RGCY 4 cut(s) 56, 64, 117, 366
CviKI_1 RGCY 4 cut(s) 56, 64, 117, 366
DpnI GATC 2 cut(s) 300, 564
DpnII GATC 2 cut(s) 298, 562
Ecl136II GAGCTC 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 66
Eco31I GGTCTC 1 cut(s) 201
Eco32I GATATC 1 cut(s) 423
Eco53kI GAGCTC 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 234
EcoRII CCWGG 2 cut(s) 176, 381
EcoRV GATATC 1 cut(s) 423
EcoT22I ATGCAT 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 66
FaeI CATG 2 cut(s) 280, 344
FaiI YATR 7 cut(s) 131, 246, 252, 278, 342, 350, 543
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FatI CATG 2 cut(s) 276, 340
FauI CCCGC 2 cut(s) 51, 141
Fnu4HI GCNGC 2 cut(s) 367, 585
FokI GGATG 2 cut(s) 191, 398
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 2 cut(s) 367, 585
GluI GCNGC 2 cut(s) 367, 585
GsuI CTGGAG 1 cut(s) 513
HaeIII GGCC 1 cut(s) 117
Hin1II CATG 2 cut(s) 280, 344
HinfI GANTC 1 cut(s) 331
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 1 cut(s) 427
Hpy188III TCNNGA 5 cut(s) 59, 341, 401, 530, 604
Hpy8I GTNNAC 1 cut(s) 361
HpyAV CCTTC 1 cut(s) 586
HpyCH4III ACNGT 1 cut(s) 213
HpyCH4IV ACGT 1 cut(s) 547
HpyCH4V TGCA 5 cut(s) 103, 312, 413, 455, 556
HpySE526I ACGT 1 cut(s) 547
Hsp92II CATG 2 cut(s) 280, 344
Kzo9I GATC 2 cut(s) 298, 562
LmnI GCTCC 1 cut(s) 532
Lsp1109I GCAGC 2 cut(s) 353, 571
LweI GCATC 4 cut(s) 66, 90, 159, 442
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 2 cut(s) 87, 436
MalI GATC 2 cut(s) 300, 564
MboI GATC 2 cut(s) 298, 562
MboII GAAGA 3 cut(s) 149, 207, 404
MfeI CAATTG 1 cut(s) 313
MflI RGATCY 1 cut(s) 562
MhlI GDGCHC 2 cut(s) 66, 529
MluCI AATT 4 cut(s) 22, 48, 140, 313
MnlI CCTC 9 cut(s) 106, 235, 248, 312, 321, 408, 424, 526, 552
Mph1103I ATGCAT 1 cut(s) 457
MseI TTAA 2 cut(s) 234, 458
MslI CAYNNNNRTG 3 cut(s) 281, 339, 384
MspA1I CMGCKG 1 cut(s) 366
MspR9I CCNGG 2 cut(s) 178, 383
MunI CAATTG 1 cut(s) 313
MvaI CCWGG 2 cut(s) 178, 383
MvnI CGCG 1 cut(s) 46
NdeII GATC 2 cut(s) 298, 562
NlaIII CATG 2 cut(s) 280, 344
NmuCI GTSAC 1 cut(s) 436
NsiI ATGCAT 1 cut(s) 457
PagI TCATGA 1 cut(s) 340
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PfeI GAWTC 1 cut(s) 331
PkrI GCNGC 2 cut(s) 368, 586
PshBI ATTAAT 1 cut(s) 458
Psp124BI GAGCTC 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 176, 381
PspGI CCWGG 2 cut(s) 176, 381
PspPI GGNCC 1 cut(s) 115
PsuI RGATCY 1 cut(s) 562
PvuII CAGCTG 1 cut(s) 366
RseI CAYNNNNRTG 3 cut(s) 281, 339, 384
SacI GAGCTC 1 cut(s) 66
SaqAI TTAA 2 cut(s) 234, 458
SatI GCNGC 2 cut(s) 367, 585
Sau3AI GATC 2 cut(s) 298, 562
Sau96I GGNCC 1 cut(s) 115
ScrFI CCNGG 2 cut(s) 178, 383
SduI GDGCHC 2 cut(s) 66, 529
SetI ASST 7 cut(s) 66, 241, 368, 518, 540, 550, 597
SfaNI GCATC 4 cut(s) 66, 90, 159, 442
SmiMI CAYNNNNRTG 3 cut(s) 281, 339, 384
SmlI CTYRAG 1 cut(s) 57
SmoI CTYRAG 1 cut(s) 57
Sse9I AATT 4 cut(s) 22, 48, 140, 313
SsiI CCGC 2 cut(s) 44, 148
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 2 cut(s) 176, 381
TaaI ACNGT 1 cut(s) 213
TaiI ACGT 1 cut(s) 550
TaqI TCGA 3 cut(s) 26, 297, 489
TasI AATT 4 cut(s) 22, 48, 140, 313
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 2 cut(s) 234, 458
Tru9I TTAA 2 cut(s) 234, 458
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 2 cut(s) 366, 584
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 5 cut(s) 17, 193, 323, 329, 558
TspRI CASTG 1 cut(s) 441
VspI ATTAAT 1 cut(s) 458
XagI CCTNNNNNAGG 1 cut(s) 234
XapI RAATTY 2 cut(s) 22, 48
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.