Rh4CG033800

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0021446
Physical Location & Seq
Reverse (-)
6941116 .. 6942537
1422 bp
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UTR
Exon/CDS
Intron
Rh4CG033800.1

Sequence Viewer

Length: 453 bp
ATGAACTCTCTAACAATGCCAAATTCGATAGATTTCCACACCCCGCGAATTTTGGCTCAAGAGCTCTGTCAGTTGATGCTGTTCAAGTTACTAATGGAGATGCAAGCAAAGGAGGGCCACTTGTTGAGTATGAGCGAAGAATTGCTGCGGGCGAACTTGTTGATGGTGATGCCTGCCAGGAGTAGATGGTTGTGGTCTCGTTTCATCCCACAGTCTTCAAATGCCTCCCCTGTTAAAGGTCTTTATCTTTATGGAGGAGTGGGAACTGGAAAAACCATGTTGATGGACTTGTTTTTCGATCAACTACAATGCAATTGGAGGAAAAAGAGGATTCATTTTCATGATTTTATGTTGAATGTCCACAGCTGCTTGCGAAAGCACCAGGGTGTAGAAGACCCTCTTGAAGTTGTTGCAGGAGAGATATCTGATGAGGCAGTCTTGATTTCATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

150

Amino Acids

17.34

Weight (kDa)

7.01

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 51 - 147 3.1e-24 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021446)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0273951
rosa_samantha Rh4CG033800 Rh6DG188500
rosa_wichuraiana Rw1G030190 Rw6G012200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 46
AciI CCGC 2 cut(s) 44, 148
AcsI RAATTY 2 cut(s) 22, 48
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 4 cut(s) 85, 219, 355, 404
AjnI CCWGG 2 cut(s) 176, 381
AleI CACNNNNGTG 1 cut(s) 384
AluBI AGCT 2 cut(s) 64, 366
AluI AGCT 2 cut(s) 64, 366
Alw21I GWGCWC 1 cut(s) 66
Alw26I GTCTC 1 cut(s) 201
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 2 cut(s) 145, 366
ApoI RAATTY 2 cut(s) 22, 48
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 178
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 2 cut(s) 207, 399
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 2 cut(s) 132, 353
BccI CCATC 3 cut(s) 157, 180, 277
BciT130I CCWGG 2 cut(s) 178, 383
BcoDI GTCTC 1 cut(s) 201
BisI GCNGC 2 cut(s) 146, 367
BlsI GCNGC 2 cut(s) 147, 368
Bme1390I CCNGG 2 cut(s) 178, 383
BmgT120I GGNCC 1 cut(s) 115
BmrFI CCNGG 2 cut(s) 178, 383
BmsI GCATC 3 cut(s) 66, 90, 159
BpiI GAAGAC 2 cut(s) 207, 399
BpuEI CTTGAG 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 382
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse1I ACTGG 1 cut(s) 271
BseBI CCWGG 2 cut(s) 178, 383
BseDI CCNNGG 1 cut(s) 382
BseGI GGATG 1 cut(s) 204
BseLI CCNNNNNNNGG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 271
BseRI GAGGAG 1 cut(s) 270
BseXI GCAGC 2 cut(s) 132, 353
Bsh1236I CGCG 1 cut(s) 46
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 66
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 1 cut(s) 201
BsnI GGCC 1 cut(s) 117
Bso31I GGTCTC 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 1 cut(s) 298
BspACI CCGC 2 cut(s) 44, 148
BspANI GGCC 1 cut(s) 117
BspFNI CGCG 1 cut(s) 46
BspHI TCATGA 1 cut(s) 340
BspTNI GGTCTC 1 cut(s) 201
BsrI ACTGG 1 cut(s) 271
BssECI CCNNGG 1 cut(s) 382
BssMI GATC 1 cut(s) 298
Bst2UI CCWGG 2 cut(s) 178, 383
Bst4CI ACNGT 1 cut(s) 213
BstC8I GCNNGC 4 cut(s) 105, 150, 174, 371
BstENI CCTNNNNNAGG 1 cut(s) 234
BstF5I GGATG 1 cut(s) 204
BstFNI CGCG 1 cut(s) 46
BstKTI GATC 1 cut(s) 301
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 1 cut(s) 298
BstNI CCWGG 2 cut(s) 178, 383
BstSCI CCNGG 2 cut(s) 176, 381
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 2 cut(s) 132, 353
BstV2I GAAGAC 2 cut(s) 207, 399
BstXI CCANNNNNNTGG 1 cut(s) 283
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 1 cut(s) 204
Cac8I GCNNGC 4 cut(s) 105, 150, 174, 371
CciI TCATGA 1 cut(s) 340
Cfr13I GGNCC 1 cut(s) 115
CviAII CATG 3 cut(s) 277, 341, 447
CviJI RGCY 4 cut(s) 56, 64, 117, 366
CviKI_1 RGCY 4 cut(s) 56, 64, 117, 366
DpnI GATC 1 cut(s) 300
DpnII GATC 1 cut(s) 298
Ecl136II GAGCTC 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 66
Eco31I GGTCTC 1 cut(s) 201
Eco32I GATATC 1 cut(s) 423
Eco53kI GAGCTC 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 234
EcoRII CCWGG 2 cut(s) 176, 381
EcoRV GATATC 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 66
FaeI CATG 3 cut(s) 280, 344, 450
FaiI YATR 6 cut(s) 131, 252, 278, 342, 350, 448
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FatI CATG 3 cut(s) 276, 340, 446
FauI CCCGC 2 cut(s) 51, 141
Fnu4HI GCNGC 2 cut(s) 146, 367
FokI GGATG 1 cut(s) 191
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 2 cut(s) 146, 367
GluI GCNGC 2 cut(s) 146, 367
HaeIII GGCC 1 cut(s) 117
Hin1II CATG 3 cut(s) 280, 344, 450
HinfI GANTC 1 cut(s) 331
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 1 cut(s) 427
Hpy188III TCNNGA 4 cut(s) 59, 341, 401, 439
Hpy8I GTNNAC 1 cut(s) 361
HpyCH4III ACNGT 1 cut(s) 213
HpyCH4V TGCA 3 cut(s) 103, 312, 413
Hsp92II CATG 3 cut(s) 280, 344, 450
Kzo9I GATC 1 cut(s) 298
LpnPI CCDG 8 cut(s) 163, 186, 190, 243, 252, 368, 395, 399
Lsp1109I GCAGC 2 cut(s) 132, 353
LweI GCATC 3 cut(s) 66, 90, 159
MaeIII GTNAC 1 cut(s) 87
MalI GATC 1 cut(s) 300
MboI GATC 1 cut(s) 298
MboII GAAGA 3 cut(s) 149, 207, 404
MfeI CAATTG 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 4 cut(s) 22, 48, 140, 313
MnlI CCTC 7 cut(s) 106, 235, 248, 312, 321, 408, 424
MseI TTAA 1 cut(s) 234
MslI CAYNNNNRTG 3 cut(s) 281, 339, 384
MspA1I CMGCKG 1 cut(s) 366
MspR9I CCNGG 2 cut(s) 178, 383
MunI CAATTG 1 cut(s) 313
MvaI CCWGG 2 cut(s) 178, 383
MvnI CGCG 1 cut(s) 46
NdeII GATC 1 cut(s) 298
NlaIII CATG 3 cut(s) 280, 344, 450
OliI CACNNNNGTG 1 cut(s) 384
PagI TCATGA 1 cut(s) 340
PfeI GAWTC 1 cut(s) 331
PkrI GCNGC 2 cut(s) 147, 368
Psp124BI GAGCTC 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 176, 381
PspGI CCWGG 2 cut(s) 176, 381
PspPI GGNCC 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 366
RseI CAYNNNNRTG 3 cut(s) 281, 339, 384
SacI GAGCTC 1 cut(s) 66
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 2 cut(s) 146, 367
Sau3AI GATC 1 cut(s) 298
Sau96I GGNCC 1 cut(s) 115
ScrFI CCNGG 2 cut(s) 178, 383
SduI GDGCHC 1 cut(s) 66
SetI ASST 3 cut(s) 66, 241, 368
SfaNI GCATC 3 cut(s) 66, 90, 159
SmiMI CAYNNNNRTG 3 cut(s) 281, 339, 384
SmlI CTYRAG 1 cut(s) 57
SmoI CTYRAG 1 cut(s) 57
Sse9I AATT 4 cut(s) 22, 48, 140, 313
SsiI CCGC 2 cut(s) 44, 148
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 2 cut(s) 176, 381
TaaI ACNGT 1 cut(s) 213
TaqI TCGA 2 cut(s) 26, 297
TasI AATT 4 cut(s) 22, 48, 140, 313
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TseI GCWGC 2 cut(s) 145, 366
TspDTI ATGAA 5 cut(s) 17, 193, 323, 329, 435
XagI CCTNNNNNAGG 1 cut(s) 234
XapI RAATTY 2 cut(s) 22, 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.