RchiOBHm_Chr6g0309051

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0025083
Physical Location & Seq
Reverse (-)
66975264 .. 66976020
757 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 687 bp
ATGGAAACTTCAATGTACTACTACATTTTGCTCTTCATTGTCTTCCTTTTCCTCAAGAACTATTTGCAGAAAACTAACAGTCAACTCCCACCATGCCCTGGTCCATCTCTACCCTTTATAGGCCATCTCTACCTCTTCCAAAAACCCTTACATAAAACACTAGCAAAACTCTCTGATAAATATGGTCCAATTGTATACATCAAATTTGGCTCCCGCCCTGTCATTCTTGTATCCTCCCCATCTGCAGTCGAGGAATGCTTCACAAAGCATGACGTTGCCTTTGCAAACCGTCCAAAGCTGCTTGCCGGAAAATACCTTGGTTATAACTATACCACAGTTACCTGGGCTTCTTATGGCTCTCATTGGCGCAACTTGAGACGTATTTCTTCCCTTGAATTATTGTCATCCAACCGCCTTCAGATGCTTCAGGGCATACGTGCAGATGAAGTCAGGTCACTCATTTGCCGGCTTTTTAGAGGGTCTAATGGTGGTGAGTTTCAGAGTTTAGAGATTAAGTCAACATTTTTTCAGCTAACTCTCAATGTTATGATGAGGATGGTAACTGGGAAGAGATGCTATGGGGAAGATATAGCCGATTTGGAGGAAGCTAAAAGGTTTCAACAAATAATAAAATATCCCAGTATTGATGGTTACGTTTTTCCCTTAAAAGAAAGGTTGACTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

228

Amino Acids

26.3

Weight (kDa)

9.57

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 198 2.5e-21 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025083)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0309051
rosa_multiflora Rmu_co8289637.1_g000001
rosa_samantha Rh6AG482000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 324
AccB7I CCANNNNNTGG 1 cut(s) 98
AccI GTMKAC 1 cut(s) 195
AciI CCGC 2 cut(s) 214, 412
AcsI RAATTY 1 cut(s) 203
AcuI CTGAAG 2 cut(s) 401, 410
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 2 cut(s) 98, 119
AgsI TTSAA 3 cut(s) 12, 395, 620
AjnI CCWGG 2 cut(s) 97, 341
AluBI AGCT 3 cut(s) 298, 532, 608
AluI AGCT 3 cut(s) 298, 532, 608
Alw26I GTCTC 1 cut(s) 370
AoxI GGCC 1 cut(s) 121
ApeKI GCWGC 1 cut(s) 298
ApoI RAATTY 1 cut(s) 203
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AspLEI GCGC 1 cut(s) 369
AspS9I GGNCC 2 cut(s) 101, 185
AsuHPI GGTGA 1 cut(s) 503
AvaII GGWCC 2 cut(s) 101, 185
BbsI GAAGAC 1 cut(s) 34
BbvI GCAGC 1 cut(s) 285
BccI CCATC 5 cut(s) 112, 132, 247, 550, 641
BciT130I CCWGG 2 cut(s) 99, 343
BciVI GTATCC 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 370
BfaI CTAG 1 cut(s) 161
BfmI CTRYAG 1 cut(s) 243
BfuI GTATCC 1 cut(s) 241
BisI GCNGC 1 cut(s) 299
BlsI GCNGC 1 cut(s) 300
Bme1390I CCNGG 2 cut(s) 99, 343
Bme18I GGWCC 2 cut(s) 101, 185
BmgT120I GGNCC 2 cut(s) 101, 185
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 2 cut(s) 99, 343
BmrI ACTGGG 2 cut(s) 573, 633
BmsI GCATC 2 cut(s) 411, 563
BmuI ACTGGG 2 cut(s) 573, 633
BpiI GAAGAC 1 cut(s) 34
BpuEI CTTGAG 2 cut(s) 38, 394
BsaAI YACGTR 1 cut(s) 437
BsaJI CCNNGG 3 cut(s) 97, 316, 342
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 119
Bse118I RCCGGY 1 cut(s) 465
Bse1I ACTGG 2 cut(s) 568, 639
BseBI CCWGG 2 cut(s) 99, 343
BseDI CCNNGG 3 cut(s) 97, 316, 342
BseGI GGATG 2 cut(s) 404, 561
BseLI CCNNNNNNNGG 2 cut(s) 98, 119
BseNI ACTGG 2 cut(s) 568, 639
BseXI GCAGC 1 cut(s) 285
BsgI GTGCAG 1 cut(s) 459
BshFI GGCC 1 cut(s) 123
BsiSI CCGG 2 cut(s) 306, 466
BslI CCNNNNNNNGG 2 cut(s) 98, 119
BsmAI GTCTC 1 cut(s) 370
BsmBI CGTCTC 1 cut(s) 370
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 1 cut(s) 123
BspACI CCGC 2 cut(s) 214, 412
BspANI GGCC 1 cut(s) 123
BspLI GGNNCC 1 cut(s) 211
BspMAI CTGCAG 1 cut(s) 247
BspQI GCTCTTC 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 465
BsrI ACTGG 2 cut(s) 568, 639
BssAI RCCGGY 1 cut(s) 465
BssECI CCNNGG 3 cut(s) 97, 316, 342
BssNAI GTATAC 1 cut(s) 196
BssT1I CCWWGG 1 cut(s) 316
Bst1107I GTATAC 1 cut(s) 196
Bst2UI CCWGG 2 cut(s) 99, 343
Bst4CI ACNGT 3 cut(s) 80, 290, 337
Bst6I CTCTTC 3 cut(s) 38, 140, 563
BstBAI YACGTR 1 cut(s) 437
BstC8I GCNNGC 2 cut(s) 303, 467
BstF5I GGATG 2 cut(s) 404, 561
BstHHI GCGC 1 cut(s) 369
BstMAI GTCTC 1 cut(s) 370
BstNI CCWGG 2 cut(s) 99, 343
BstSCI CCNGG 2 cut(s) 97, 341
BstSFI CTRYAG 1 cut(s) 243
BstV1I GCAGC 1 cut(s) 285
BstV2I GAAGAC 1 cut(s) 34
BstZ17I GTATAC 1 cut(s) 196
BsuI GTATCC 1 cut(s) 241
BsuRI GGCC 1 cut(s) 123
BtsCI GGATG 2 cut(s) 404, 561
Cac8I GCNNGC 2 cut(s) 303, 467
CfoI GCGC 1 cut(s) 369
Cfr10I RCCGGY 1 cut(s) 465
Cfr13I GGNCC 2 cut(s) 101, 185
Csp6I GTAC 1 cut(s) 16
CviAII CATG 2 cut(s) 93, 269
CviJI RGCY 9 cut(s) 123, 210, 298, 347, 357, 469, 532, 593, 608
CviKI_1 RGCY 9 cut(s) 123, 210, 298, 347, 357, 469, 532, 593, 608
CviQI GTAC 1 cut(s) 16
Eam1104I CTCTTC 3 cut(s) 38, 140, 563
EarI CTCTTC 3 cut(s) 38, 140, 563
Eco130I CCWWGG 1 cut(s) 316
Eco47I GGWCC 2 cut(s) 101, 185
Eco57I CTGAAG 2 cut(s) 401, 410
EcoRII CCWGG 2 cut(s) 97, 341
EcoT14I CCWWGG 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 316
Esp3I CGTCTC 1 cut(s) 370
FaeI CATG 2 cut(s) 96, 272
FalI AAGNNNNNCTT 1 cut(s) 664
FatI CATG 2 cut(s) 92, 268
FauI CCCGC 1 cut(s) 221
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 299
FokI GGATG 2 cut(s) 391, 568
Fsp4HI GCNGC 1 cut(s) 299
FspBI CTAG 1 cut(s) 161
GlaI GCGC 1 cut(s) 368
GluI GCNGC 1 cut(s) 299
HaeIII GGCC 1 cut(s) 123
HapII CCGG 2 cut(s) 306, 466
HhaI GCGC 1 cut(s) 369
Hin1II CATG 2 cut(s) 96, 272
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HincII GTYRAC 3 cut(s) 83, 519, 678
HindII GTYRAC 3 cut(s) 83, 519, 678
HpaII CCGG 2 cut(s) 306, 466
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 4 cut(s) 83, 196, 519, 678
Hpy188I TCNGA 3 cut(s) 175, 420, 501
Hpy188III TCNNGA 2 cut(s) 55, 684
Hpy8I GTNNAC 4 cut(s) 83, 196, 519, 678
HpyAV CCTTC 1 cut(s) 425
HpyCH4III ACNGT 3 cut(s) 80, 290, 337
HpyCH4IV ACGT 4 cut(s) 273, 379, 436, 654
HpyCH4V TGCA 4 cut(s) 67, 245, 284, 440
HpySE526I ACGT 4 cut(s) 273, 379, 436, 654
Hsp92II CATG 2 cut(s) 96, 272
HspAI GCGC 1 cut(s) 367
KroI GCCGGC 1 cut(s) 465
KroNI GCCGGC 1 cut(s) 467
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 1 cut(s) 215
Lsp1109I GCAGC 1 cut(s) 285
LweI GCATC 2 cut(s) 411, 563
MaeI CTAG 1 cut(s) 161
MaeII ACGT 4 cut(s) 273, 379, 436, 654
MaeIII GTNAC 4 cut(s) 337, 453, 559, 650
MboII GAAGA 6 cut(s) 25, 34, 127, 378, 580, 596
MfeI CAATTG 1 cut(s) 189
MluCI AATT 3 cut(s) 189, 203, 395
MmeI TCCRAC 1 cut(s) 432
MnlI CCTC 7 cut(s) 62, 143, 244, 244, 470, 546, 595
MroNI GCCGGC 1 cut(s) 465
MseI TTAA 2 cut(s) 513, 665
MspI CCGG 2 cut(s) 306, 466
MspR9I CCNGG 2 cut(s) 99, 343
MunI CAATTG 1 cut(s) 189
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 2 cut(s) 99, 343
NaeI GCCGGC 1 cut(s) 467
NgoMIV GCCGGC 1 cut(s) 465
NlaIII CATG 2 cut(s) 96, 272
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 453
PciSI GCTCTTC 1 cut(s) 38
PctI GAATGC 1 cut(s) 260
PdiI GCCGGC 1 cut(s) 467
PflMI CCANNNNNTGG 1 cut(s) 98
PkrI GCNGC 1 cut(s) 300
Ppu21I YACGTR 1 cut(s) 437
PsiI TTATAA 1 cut(s) 324
Psp6I CCWGG 2 cut(s) 97, 341
PspGI CCWGG 2 cut(s) 97, 341
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 2 cut(s) 101, 185
PstI CTGCAG 1 cut(s) 247
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
SapI GCTCTTC 1 cut(s) 38
SaqAI TTAA 2 cut(s) 513, 665
SatI GCNGC 1 cut(s) 299
Sau96I GGNCC 2 cut(s) 101, 185
ScrFI CCNGG 2 cut(s) 99, 343
SfaNI GCATC 2 cut(s) 411, 563
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 2 cut(s) 101, 185
SmlI CTYRAG 2 cut(s) 53, 373
SmoI CTYRAG 2 cut(s) 53, 373
Sse9I AATT 3 cut(s) 189, 203, 395
SsiI CCGC 2 cut(s) 214, 412
SspMI CTAG 1 cut(s) 161
StyD4I CCNGG 2 cut(s) 97, 341
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 3 cut(s) 80, 290, 337
TaiI ACGT 4 cut(s) 276, 382, 439, 657
TaqI TCGA 1 cut(s) 249
TasI AATT 3 cut(s) 189, 203, 395
TatI WGTACW 1 cut(s) 15
Tru1I TTAA 2 cut(s) 513, 665
Tru9I TTAA 2 cut(s) 513, 665
TseFI GTSAC 1 cut(s) 453
TseI GCWGC 1 cut(s) 298
Tsp45I GTSAC 1 cut(s) 453
TspDTI ATGAA 2 cut(s) 25, 459
Van91I CCANNNNNTGG 1 cut(s) 98
VpaK11BI GGWCC 2 cut(s) 101, 185
XapI RAATTY 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.