RchiOBHm_Chr5g0032031

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0030168
Physical Location & Seq
Reverse (-)
25714866 .. 25718480
3615 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 492 bp
ATGCACTCACGGTCTTTGACGATGAGTTCACTGATAGAAGGTCATCAGCTTGGCTCTGGCTTGTTGGGCACGGCACAAGGATTTTACGTCGCTAGTTCCAAAGATGGGAGTAGCCAGACCATTGCTTTCACGGCAATGTTTGAGAGTGGTGGATTTTGTAGAAGTTCAGGTACTGGAGTAGAAAAGAGAAGAGGACAATCGGTTTGCGTTTGTTTTGGAAAAGATTGTGGTGATAGAGGCTGCGATTCTAATTGGGAATCGGAGGTGTTGGTGGTGGTTGTGGAGGCGGCGGAGGAGTATGGTTCCAGTGAAGGAAGACAGGGTTCTGATAGAGAGGTGGCTGTAGTCTCTCACCTTGCAGTTATGGGAGGGACCAGGAAGTACTTGAATGCAAAAGGGTATGCTGTGGTTAAGACCATTAATGGAGTGAATGGGCAAAATACTGATGGAATTGACACTGTTCTTCAGTTTACTGTGTATCTTAGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

163

Amino Acids

17.25

Weight (kDa)

5.58

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 92 - 161 2.4e-06 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0030168)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0032031
rosa_samantha Rh5DG228400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 287, 290
AcuI CTGAAG 1 cut(s) 449
AfaI GTAC 2 cut(s) 172, 383
AfiI CCNNNNNNNGG 1 cut(s) 105
AgsI TTSAA 1 cut(s) 388
AjnI CCWGG 1 cut(s) 374
AluBI AGCT 1 cut(s) 49
AluI AGCT 1 cut(s) 49
Alw26I GTCTC 1 cut(s) 352
AlwNI CAGNNNCTG 1 cut(s) 173
ApeKI GCWGC 1 cut(s) 240
ArsI GACNNNNNNTTYG 2 cut(s) 186, 218
AseI ATTAAT 1 cut(s) 420
AspS9I GGNCC 1 cut(s) 372
AsuHPI GGTGA 2 cut(s) 242, 344
AvaII GGWCC 1 cut(s) 372
BaeGI GKGCMC 1 cut(s) 71
BaeI ACNNNNGTAYC 2 cut(s) 162, 195
BarI GAAGNNNNNNTAC 2 cut(s) 154, 186
BbsI GAAGAC 1 cut(s) 322
BbvI GCAGC 1 cut(s) 227
BccI CCATC 2 cut(s) 98, 440
BceAI ACGGC 2 cut(s) 87, 147
BciT130I CCWGG 1 cut(s) 376
BcoDI GTCTC 1 cut(s) 352
BfaI CTAG 2 cut(s) 93, 490
BfmI CTRYAG 1 cut(s) 342
BisI GCNGC 2 cut(s) 241, 288
BlsI GCNGC 2 cut(s) 242, 289
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 1 cut(s) 376
Bme18I GGWCC 1 cut(s) 372
BmgT120I GGNCC 1 cut(s) 372
BmiI GGNNCC 2 cut(s) 304, 373
BmrFI CCNGG 1 cut(s) 376
BpiI GAAGAC 1 cut(s) 322
BpmI CTGGAG 1 cut(s) 195
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 2 cut(s) 178, 306
Bse3DI GCAATG 2 cut(s) 120, 141
BseBI CCWGG 1 cut(s) 376
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMI GCAATG 2 cut(s) 120, 141
BseNI ACTGG 2 cut(s) 178, 306
BseRI GAGGAG 1 cut(s) 308
BseSI GKGCMC 1 cut(s) 71
BseXI GCAGC 1 cut(s) 227
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 1 cut(s) 105
BsmAI GTCTC 1 cut(s) 352
BsmFI GGGAC 1 cut(s) 385
BsmI GAATGC 1 cut(s) 394
Bsp1286I GDGCHC 1 cut(s) 71
BspACI CCGC 2 cut(s) 287, 290
BspLI GGNNCC 2 cut(s) 304, 373
BsrDI GCAATG 2 cut(s) 120, 141
BsrI ACTGG 2 cut(s) 178, 306
Bst2UI CCWGG 1 cut(s) 376
Bst4CI ACNGT 3 cut(s) 12, 460, 475
Bst6I CTCTTC 1 cut(s) 184
BstDEI CTNAG 1 cut(s) 482
BstMAI GTCTC 1 cut(s) 352
BstMWI GCNNNNNNNGC 2 cut(s) 66, 131
BstNI CCWGG 1 cut(s) 376
BstSCI CCNGG 1 cut(s) 374
BstSFI CTRYAG 1 cut(s) 342
BstSLI GKGCMC 1 cut(s) 71
BstV1I GCAGC 1 cut(s) 227
BstV2I GAAGAC 1 cut(s) 322
BtsIMutI CAGTG 3 cut(s) 29, 313, 456
CaiI CAGNNNCTG 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 372
Csp6I GTAC 2 cut(s) 171, 382
CviJI RGCY 6 cut(s) 49, 54, 60, 114, 240, 341
CviKI_1 RGCY 6 cut(s) 49, 54, 60, 114, 240, 341
CviQI GTAC 2 cut(s) 171, 382
DdeI CTNAG 1 cut(s) 482
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
EciI GGCGGA 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 372
Eco57I CTGAAG 1 cut(s) 449
EcoRII CCWGG 1 cut(s) 374
FaiI YATR 3 cut(s) 300, 365, 402
FaqI GGGAC 1 cut(s) 385
Fnu4HI GCNGC 2 cut(s) 241, 288
Fsp4HI GCNGC 2 cut(s) 241, 288
FspBI CTAG 2 cut(s) 93, 490
GluI GCNGC 2 cut(s) 241, 288
GsuI CTGGAG 1 cut(s) 195
HinfI GANTC 2 cut(s) 245, 257
HphI GGTGA 2 cut(s) 242, 344
Hpy166II GTNNAC 2 cut(s) 29, 471
Hpy188I TCNGA 2 cut(s) 262, 328
Hpy8I GTNNAC 2 cut(s) 29, 471
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 2 cut(s) 32, 305
HpyCH4III ACNGT 3 cut(s) 12, 460, 475
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 3 cut(s) 4, 359, 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 131
HpyF3I CTNAG 1 cut(s) 482
HpySE526I ACGT 1 cut(s) 87
LpnPI CCDG 8 cut(s) 42, 128, 153, 159, 305, 319, 361, 388
Lsp1109I GCAGC 1 cut(s) 227
MaeI CTAG 2 cut(s) 93, 490
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 1 cut(s) 485
MboII GAAGA 3 cut(s) 201, 327, 455
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 2 cut(s) 250, 450
MnlI CCTC 7 cut(s) 185, 230, 256, 277, 286, 328, 362
MseI TTAA 2 cut(s) 411, 420
MslI CAYNNNNRTG 1 cut(s) 134
MspR9I CCNGG 1 cut(s) 376
Mva1269I GAATGC 1 cut(s) 394
MvaI CCWGG 1 cut(s) 376
MwoI GCNNNNNNNGC 2 cut(s) 66, 131
NlaIV GGNNCC 2 cut(s) 304, 373
PctI GAATGC 1 cut(s) 394
PfeI GAWTC 2 cut(s) 245, 257
PkrI GCNGC 2 cut(s) 242, 289
PshBI ATTAAT 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 374
PspGI CCWGG 1 cut(s) 374
PspN4I GGNNCC 2 cut(s) 304, 373
PspPI GGNCC 1 cut(s) 372
PstNI CAGNNNCTG 1 cut(s) 173
RsaI GTAC 2 cut(s) 172, 383
RsaNI GTAC 2 cut(s) 171, 382
RseI CAYNNNNRTG 1 cut(s) 134
SaqAI TTAA 2 cut(s) 411, 420
SatI GCNGC 2 cut(s) 241, 288
Sau96I GGNCC 1 cut(s) 372
ScaI AGTACT 1 cut(s) 383
ScrFI CCNGG 1 cut(s) 376
SduI GDGCHC 1 cut(s) 71
SetI ASST 7 cut(s) 43, 51, 90, 172, 267, 339, 357
SfcI CTRYAG 1 cut(s) 342
SinI GGWCC 1 cut(s) 372
SmiMI CAYNNNNRTG 1 cut(s) 134
Sse9I AATT 2 cut(s) 250, 450
SsiI CCGC 2 cut(s) 287, 290
SspMI CTAG 2 cut(s) 93, 490
StyD4I CCNGG 1 cut(s) 374
TaaI ACNGT 3 cut(s) 12, 460, 475
TaiI ACGT 1 cut(s) 90
TasI AATT 2 cut(s) 250, 450
TatI WGTACW 1 cut(s) 381
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 2 cut(s) 245, 257
Tru1I TTAA 2 cut(s) 411, 420
Tru9I TTAA 2 cut(s) 411, 420
TscAI CASTG 3 cut(s) 36, 313, 463
TseI GCWGC 1 cut(s) 240
TspRI CASTG 3 cut(s) 36, 313, 463
VpaK11BI GGWCC 1 cut(s) 372
VspI ATTAAT 1 cut(s) 420
XspI CTAG 2 cut(s) 93, 490
ZrmI AGTACT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.