RchiOBHm_Chr4g0414921

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0030097
Physical Location & Seq
Forward (+)
39444971 .. 39446638
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 780 bp
ATGTACTTTTCTCTCTTCTCCTCCATTTCTAAAGTTCATGAATTGTCAATTATTGTTGTTTATTTGGGTTGGATCTCATATCCGATTAGCACATTCTTGAGGAATATTAATTGTTATCTCATGAGAAAATTACAGGTTGCTACTATTGTTATACTGGGATTACTATTCCTACAAGCGGCAGAATGTGGAATACAGAATATTAAGGATGTAGTTACGTACTCCGCCATTAACTGCCGGAAGCACAGTGCTCTTTTGACTGATTTCGGAGCTGTTGGCGATGGAAAGACATTGAACACAAAGGCCTTTAAAGATGCAGTTGATAAACTAAGCCAGTATGCATCCGATGGTGGAGCACAGCTAATTGTGCCCCCCGGTAACTGGCTTACCGGGAGCTTCAACCTCACTAGCCACTTTACCCTTTTCCTCCACAAGGATGCTGTTATTCTTGGGTCTCAGGATGAGTCCGAGTGGCCTCTTCTTCCAGCACTGCCTTCATACGGGCAAGGGAGAGAGGGCCCTGGTGGAAGGTTAAGCAGTTTGATCGTTGGAACAAACCTTACCGACGTCGTAATTACCGGTAACAATGGCACCATTGATGGGCAAGGCGCTCCCTGGTGGGAGAAATTCAAGAAGGGTCTTTTGAATGTCACCCGACCATACTTGGTTGAGATTATGTACTCTAATAAGATCCAAATCTCCAATCTCACTTTTACGAATTCTCCATCCTGGCACATCCATCCCATTTACAGCAGGTTAGATCTTTTTCATATCTTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

259

Amino Acids

28.67

Weight (kDa)

8.36

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 179 - 246 3.4e-07 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030097)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0414921
rosa_samantha Rh1AG429600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 567
Acc36I ACCTGC 1 cut(s) 741
AccB1I GGYRCC 1 cut(s) 587
AciI CCGC 2 cut(s) 176, 222
AclWI GGATC 2 cut(s) 80, 682
AcsI RAATTY 2 cut(s) 623, 715
AcyI GRCGYC 1 cut(s) 564
AfaI GTAC 3 cut(s) 5, 218, 677
AfiI CCNNNNNNNGG 5 cut(s) 175, 378, 430, 497, 597
AgeI ACCGGT 1 cut(s) 575
AgsI TTSAA 5 cut(s) 292, 397, 628, 643, 775
AjnI CCWGG 3 cut(s) 517, 611, 725
AluBI AGCT 3 cut(s) 269, 358, 393
AluI AGCT 3 cut(s) 269, 358, 393
Alw21I GWGCWC 2 cut(s) 250, 355
Alw26I GTCTC 1 cut(s) 456
AlwI GGATC 2 cut(s) 80, 682
AoxI GGCC 3 cut(s) 300, 470, 514
ApaI GGGCCC 1 cut(s) 518
ApoI RAATTY 2 cut(s) 623, 715
AseI ATTAAT 1 cut(s) 108
AsiGI ACCGGT 1 cut(s) 575
AspLEI GCGC 1 cut(s) 608
AspS9I GGNCC 2 cut(s) 514, 515
AsuC2I CCSGG 2 cut(s) 372, 388
AsuHPI GGTGA 1 cut(s) 640
BaeGI GKGCMC 2 cut(s) 369, 518
BanI GGYRCC 1 cut(s) 587
BanII GRGCYC 1 cut(s) 518
Bbv12I GWGCWC 2 cut(s) 250, 355
BccI CCATC 5 cut(s) 272, 338, 590, 730, 744
BcgI CGANNNNNNTGC 2 cut(s) 523, 557
BciT130I CCWGG 3 cut(s) 519, 613, 727
BcnI CCSGG 2 cut(s) 372, 388
BcoDI GTCTC 1 cut(s) 456
BfaI CTAG 1 cut(s) 405
BfoI RGCGCY 1 cut(s) 609
BfuAI ACCTGC 1 cut(s) 741
BglII AGATCT 1 cut(s) 757
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 5 cut(s) 372, 388, 519, 613, 727
BmgT120I GGNCC 2 cut(s) 514, 515
BmiI GGNNCC 2 cut(s) 516, 589
BmrFI CCNGG 5 cut(s) 372, 388, 519, 613, 727
BmrI ACTGGG 1 cut(s) 164
BmsI GCATC 3 cut(s) 301, 347, 424
BmuI ACTGGG 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 118
BpuMI CCSGG 2 cut(s) 372, 388
BsaAI YACGTR 1 cut(s) 216
BsaBI GATNNNNATC 1 cut(s) 692
BsaHI GRCGYC 1 cut(s) 564
BsaI GGTCTC 1 cut(s) 456
BsaJI CCNNGG 3 cut(s) 370, 517, 611
BsaWI WCCGGW 1 cut(s) 575
BsaXI ACNNNNNCTCC 2 cut(s) 703, 733
Bsc4I CCNNNNNNNGG 5 cut(s) 175, 378, 430, 497, 597
Bse118I RCCGGY 1 cut(s) 575
Bse1I ACTGG 3 cut(s) 159, 331, 383
Bse8I GATNNNNATC 1 cut(s) 692
BseBI CCWGG 3 cut(s) 519, 613, 727
BseDI CCNNGG 3 cut(s) 370, 517, 611
BseGI GGATG 7 cut(s) 211, 338, 439, 463, 722, 732, 736
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 5 cut(s) 175, 378, 430, 497, 597
BseMII CTCAG 1 cut(s) 467
BseNI ACTGG 3 cut(s) 159, 331, 383
BseRI GAGGAG 1 cut(s) 10
BseSI GKGCMC 2 cut(s) 369, 518
BshFI GGCC 3 cut(s) 302, 472, 516
BshNI GGYRCC 1 cut(s) 587
BshTI ACCGGT 1 cut(s) 575
BsiHKAI GWGCWC 2 cut(s) 250, 355
BsiSI CCGG 4 cut(s) 235, 372, 387, 576
BslI CCNNNNNNNGG 5 cut(s) 175, 378, 430, 497, 597
BsmAI GTCTC 1 cut(s) 456
BsnI GGCC 3 cut(s) 302, 472, 516
Bso31I GGTCTC 1 cut(s) 456
Bsp120I GGGCCC 1 cut(s) 514
Bsp1286I GDGCHC 4 cut(s) 250, 355, 369, 518
Bsp143I GATC 4 cut(s) 72, 540, 687, 757
BspACI CCGC 2 cut(s) 176, 222
BspANI GGCC 3 cut(s) 302, 472, 516
BspCNI CTCAG 1 cut(s) 466
BspHI TCATGA 2 cut(s) 37, 120
BspLI GGNNCC 2 cut(s) 516, 589
BspMI ACCTGC 1 cut(s) 741
BspPI GGATC 2 cut(s) 80, 682
BspT107I GGYRCC 1 cut(s) 587
BspTNI GGTCTC 1 cut(s) 456
BsrFI RCCGGY 1 cut(s) 575
BsrI ACTGG 3 cut(s) 159, 331, 383
BssAI RCCGGY 1 cut(s) 575
BssECI CCNNGG 3 cut(s) 370, 517, 611
BssMI GATC 4 cut(s) 72, 540, 687, 757
BssNI GRCGYC 1 cut(s) 564
Bst2UI CCWGG 3 cut(s) 519, 613, 727
Bst4CI ACNGT 1 cut(s) 245
Bst6I CTCTTC 2 cut(s) 20, 480
BstACI GRCGYC 1 cut(s) 564
BstBAI YACGTR 1 cut(s) 216
BstDEI CTNAG 2 cut(s) 326, 453
BstENI CCTNNNNNAGG 1 cut(s) 428
BstF5I GGATG 7 cut(s) 211, 338, 439, 463, 722, 732, 736
BstH2I RGCGCY 1 cut(s) 609
BstHHI GCGC 1 cut(s) 608
BstKTI GATC 4 cut(s) 75, 543, 690, 760
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 4 cut(s) 72, 540, 687, 757
BstMWI GCNNNNNNNGC 1 cut(s) 364
BstNI CCWGG 3 cut(s) 519, 613, 727
BstSCI CCNGG 5 cut(s) 370, 386, 517, 611, 725
BstSLI GKGCMC 2 cut(s) 369, 518
BstSNI TACGTA 1 cut(s) 216
BstX2I RGATCY 3 cut(s) 72, 687, 757
BstYI RGATCY 3 cut(s) 72, 687, 757
BsuRI GGCC 3 cut(s) 302, 472, 516
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 7 cut(s) 211, 338, 439, 463, 722, 732, 736
BtsI GCAGTG 1 cut(s) 485
BtsIMutI CAGTG 2 cut(s) 250, 485
BveI ACCTGC 1 cut(s) 741
CciI TCATGA 2 cut(s) 37, 120
CfoI GCGC 1 cut(s) 608
Cfr10I RCCGGY 1 cut(s) 575
Cfr13I GGNCC 2 cut(s) 514, 515
Csp6I GTAC 3 cut(s) 4, 217, 676
CspAI ACCGGT 1 cut(s) 575
CviAII CATG 2 cut(s) 38, 121
CviJI RGCY 9 cut(s) 269, 302, 330, 358, 382, 393, 408, 472, 516
CviKI_1 RGCY 9 cut(s) 269, 302, 330, 358, 382, 393, 408, 472, 516
CviQI GTAC 3 cut(s) 4, 217, 676
DdeI CTNAG 2 cut(s) 326, 453
DpnI GATC 4 cut(s) 74, 542, 689, 759
DpnII GATC 4 cut(s) 72, 540, 687, 757
DraI TTTAAA 1 cut(s) 307
Eam1104I CTCTTC 2 cut(s) 20, 480
EarI CTCTTC 2 cut(s) 20, 480
EciI GGCGGA 1 cut(s) 211
Eco105I TACGTA 1 cut(s) 216
Eco147I AGGCCT 1 cut(s) 302
Eco24I GRGCYC 1 cut(s) 518
Eco31I GGTCTC 1 cut(s) 456
EcoNI CCTNNNNNAGG 1 cut(s) 428
EcoO109I RGGNCCY 2 cut(s) 514, 515
EcoRI GAATTC 1 cut(s) 715
EcoRII CCWGG 3 cut(s) 517, 611, 725
EcoT22I ATGCAT 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 518
FaeI CATG 2 cut(s) 41, 124
FaiI YATR 9 cut(s) 39, 79, 122, 152, 336, 496, 658, 674, 768
FatI CATG 2 cut(s) 37, 120
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 7 cut(s) 218, 325, 446, 470, 709, 719, 723
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 1 cut(s) 405
GlaI GCGC 1 cut(s) 607
GluI GCNGC 1 cut(s) 177
HaeII RGCGCY 1 cut(s) 609
HaeIII GGCC 3 cut(s) 302, 472, 516
HapII CCGG 4 cut(s) 235, 372, 387, 576
HhaI GCGC 1 cut(s) 608
Hin1I GRCGYC 1 cut(s) 564
Hin1II CATG 2 cut(s) 41, 124
Hin6I GCGC 1 cut(s) 606
HinP1I GCGC 1 cut(s) 606
HinfI GANTC 1 cut(s) 461
HpaII CCGG 4 cut(s) 235, 372, 387, 576
HphI GGTGA 1 cut(s) 640
Hpy188I TCNGA 4 cut(s) 84, 266, 343, 466
Hpy188III TCNNGA 6 cut(s) 38, 97, 121, 455, 628, 772
Hpy99I CGWCG 2 cut(s) 566, 569
HpyAV CCTTC 3 cut(s) 501, 519, 625
HpyCH4III ACNGT 1 cut(s) 245
HpyCH4IV ACGT 2 cut(s) 215, 564
HpyCH4V TGCA 2 cut(s) 314, 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 364
HpyF3I CTNAG 2 cut(s) 326, 453
HpySE526I ACGT 2 cut(s) 215, 564
Hsp92I GRCGYC 1 cut(s) 564
Hsp92II CATG 2 cut(s) 41, 124
HspAI GCGC 1 cut(s) 606
Kzo9I GATC 4 cut(s) 72, 540, 687, 757
LmnI GCTCC 4 cut(s) 266, 350, 390, 613
LweI GCATC 3 cut(s) 301, 347, 424
MaeI CTAG 1 cut(s) 405
MaeII ACGT 2 cut(s) 215, 564
MaeIII GTNAC 4 cut(s) 211, 374, 578, 646
MalI GATC 4 cut(s) 74, 542, 689, 759
MboI GATC 4 cut(s) 72, 540, 687, 757
MboII GAAGA 3 cut(s) 7, 467, 470
MflI RGATCY 3 cut(s) 72, 687, 757
MhlI GDGCHC 4 cut(s) 250, 355, 369, 518
MluCI AATT 8 cut(s) 41, 48, 109, 128, 360, 570, 623, 715
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 2 cut(s) 50, 526
MnlI CCTC 6 cut(s) 31, 93, 410, 434, 483, 505
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 5 cut(s) 108, 201, 228, 306, 530
MslI CAYNNNNRTG 1 cut(s) 432
MspI CCGG 4 cut(s) 235, 372, 387, 576
MspR9I CCNGG 5 cut(s) 372, 388, 519, 613, 727
MvaI CCWGG 3 cut(s) 519, 613, 727
MwoI GCNNNNNNNGC 1 cut(s) 364
NciI CCSGG 2 cut(s) 372, 388
NdeII GATC 4 cut(s) 72, 540, 687, 757
NlaIII CATG 2 cut(s) 41, 124
NlaIV GGNNCC 2 cut(s) 516, 589
NmuCI GTSAC 1 cut(s) 646
NsiI ATGCAT 1 cut(s) 340
PagI TCATGA 2 cut(s) 37, 120
PceI AGGCCT 1 cut(s) 302
PinAI ACCGGT 1 cut(s) 575
PkrI GCNGC 1 cut(s) 178
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
Ppu21I YACGTR 1 cut(s) 216
PshBI ATTAAT 1 cut(s) 108
Psp6I CCWGG 3 cut(s) 517, 611, 725
PspGI CCWGG 3 cut(s) 517, 611, 725
PspN4I GGNNCC 2 cut(s) 516, 589
PspOMI GGGCCC 1 cut(s) 514
PspPI GGNCC 2 cut(s) 514, 515
PsrI GAACNNNNNNTAC 2 cut(s) 541, 573
PsuI RGATCY 3 cut(s) 72, 687, 757
RsaI GTAC 3 cut(s) 5, 218, 677
RsaNI GTAC 3 cut(s) 4, 217, 676
RseI CAYNNNNRTG 1 cut(s) 432
SaqAI TTAA 5 cut(s) 108, 201, 228, 306, 530
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 4 cut(s) 72, 540, 687, 757
Sau96I GGNCC 2 cut(s) 514, 515
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 5 cut(s) 372, 388, 519, 613, 727
SduI GDGCHC 4 cut(s) 250, 355, 369, 518
SfaNI GCATC 3 cut(s) 301, 347, 424
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 1 cut(s) 97
SmoI CTYRAG 1 cut(s) 97
SnaBI TACGTA 1 cut(s) 216
Sse9I AATT 8 cut(s) 41, 48, 109, 128, 360, 570, 623, 715
SseBI AGGCCT 1 cut(s) 302
SsiI CCGC 2 cut(s) 176, 222
SspI AATATT 2 cut(s) 106, 199
SspMI CTAG 1 cut(s) 405
StuI AGGCCT 1 cut(s) 302
StyD4I CCNGG 5 cut(s) 370, 386, 517, 611, 725
TaaI ACNGT 1 cut(s) 245
TaiI ACGT 2 cut(s) 218, 567
TasI AATT 8 cut(s) 41, 48, 109, 128, 360, 570, 623, 715
TatI WGTACW 2 cut(s) 3, 675
TauI GCSGC 1 cut(s) 179
Tru1I TTAA 5 cut(s) 108, 201, 228, 306, 530
Tru9I TTAA 5 cut(s) 108, 201, 228, 306, 530
TscAI CASTG 2 cut(s) 250, 492
TseFI GTSAC 1 cut(s) 646
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 4 cut(s) 26, 54, 483, 755
TspRI CASTG 2 cut(s) 250, 492
VspI ATTAAT 1 cut(s) 108
XagI CCTNNNNNAGG 1 cut(s) 428
XapI RAATTY 2 cut(s) 623, 715
XspI CTAG 1 cut(s) 405
ZraI GACGTC 1 cut(s) 565
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.