RchiOBHm_Chr4g0418531

Mediator of RNA polymerase II transcription subunit 33A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0030033
Physical Location & Seq
Reverse (-)
43985325 .. 43986769
1445 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
ATGGCTTTTTTCGAGCAAAGCAGCCTCTGGGATAATGTCATACAGGAAACCAAGGAGGCTCAAGAAAAGGGTAGTGATCCTTTGCTCTGGTCAGTGGAAGTCTCGTCGATTTTGAGCTCGGCAGGGGTGTCCCTGCCCTCAACGGAGCTCGCCAATGTGATGGTCTCATACATTTGTTGGGAAAACAATGTGCCCATTTTGTGGAAGTTTCTCGAAAAGGCCATGGTATACAACATGGTGCCCTCTATGCTTGTTCTTGCACTGCTTTCATCCAGGGTTATTCCGAGTCGACGATTCCAGCCTGCAGCTTATAGACTTTACATGGAGCTCCTAAAGAGGCATGCTTTCTCACTCAGATCTCAGATAAATTGGCCTAATTATCAAATGGTTATGAAATCAATAGATGATATCCTTGATTTGTCCCAGACTTTTGGTCTTCAAACTAGTGATGCTGGGACTATTGTCGTTGGGTTCATCTTCTCAACTGTGTGGCAGTTGATTGATGCATCATTGGATGATGAGGGGTTGCTGGAACATGATGACCCAGAAAAGAAGTCTAGATGGGTAATTAAACCCCAAGAAATGGAGATAGATGGTTACGAGAAGGGTAATGAACGCTATGAGAGATTGCAGAGTCGGAATACTGTGATGGCTATTGAGTTAATCACACAATTTCTGCAAAACAAGTTGACTTCCAAGATTCTGAACTTGGCAAAACAAAACATGTACACATCCTTATTCAAACTTTATGGATTGTGTGGTTATTGTGTCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

29.65

Weight (kDa)

5.11

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030033)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0418531
rosa_multiflora Rmu_co8409013.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 238
AccB7I CCANNNNNTGG 2 cut(s) 201, 583
AccI GTMKAC 2 cut(s) 228, 289
AclWI GGATC 1 cut(s) 71
AfaI GTAC 1 cut(s) 728
AfiI CCNNNNNNNGG 2 cut(s) 201, 583
AflIII ACRYGT 1 cut(s) 723
AgsI TTSAA 2 cut(s) 440, 742
AhdI GACNNNNNGTC 1 cut(s) 432
AhlI ACTAGT 1 cut(s) 443
AjnI CCWGG 1 cut(s) 272
AluBI AGCT 4 cut(s) 117, 148, 308, 328
AluI AGCT 4 cut(s) 117, 148, 308, 328
Alw21I GWGCWC 3 cut(s) 119, 150, 330
Alw26I GTCTC 2 cut(s) 106, 169
AlwI GGATC 1 cut(s) 71
AlwNI CAGNNNCTG 1 cut(s) 27
AoxI GGCC 2 cut(s) 219, 371
ApeKI GCWGC 2 cut(s) 21, 305
BaeGI GKGCMC 2 cut(s) 195, 243
BanI GGYRCC 1 cut(s) 238
BanII GRGCYC 3 cut(s) 119, 150, 330
BbsI GAAGAC 1 cut(s) 428
Bbv12I GWGCWC 3 cut(s) 119, 150, 330
BbvI GCAGC 2 cut(s) 33, 317
BccI CCATC 4 cut(s) 154, 555, 587, 643
BciT130I CCWGG 1 cut(s) 274
BcoDI GTCTC 2 cut(s) 106, 169
BcuI ACTAGT 1 cut(s) 443
BfaI CTAG 2 cut(s) 444, 558
BfmI CTRYAG 1 cut(s) 303
BglII AGATCT 1 cut(s) 356
BisI GCNGC 2 cut(s) 22, 306
BlsI GCNGC 2 cut(s) 23, 307
Bme1390I CCNGG 1 cut(s) 274
BmeRI GACNNNNNGTC 1 cut(s) 432
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 1 cut(s) 274
BmsI GCATC 3 cut(s) 439, 493, 515
BoxI GACNNNNGTC 1 cut(s) 461
BpiI GAAGAC 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 169
BsaJI CCNNGG 3 cut(s) 51, 222, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 583
BseBI CCWGG 1 cut(s) 274
BseDI CCNNGG 3 cut(s) 51, 222, 273
BseGI GGATG 3 cut(s) 269, 520, 731
BseLI CCNNNNNNNGG 2 cut(s) 201, 583
BseMII CTCAG 2 cut(s) 367, 374
BseSI GKGCMC 2 cut(s) 195, 243
BseXI GCAGC 2 cut(s) 33, 317
BseYI CCCAGC 1 cut(s) 452
BshFI GGCC 2 cut(s) 221, 373
BshNI GGYRCC 1 cut(s) 238
BsiHKAI GWGCWC 3 cut(s) 119, 150, 330
BslFI GGGAC 3 cut(s) 115, 406, 469
BslI CCNNNNNNNGG 2 cut(s) 201, 583
BsmAI GTCTC 2 cut(s) 106, 169
BsmFI GGGAC 3 cut(s) 115, 406, 469
BsnI GGCC 2 cut(s) 221, 373
Bso31I GGTCTC 1 cut(s) 169
Bsp1286I GDGCHC 5 cut(s) 119, 150, 195, 243, 330
Bsp1407I TGTACA 1 cut(s) 726
Bsp143I GATC 2 cut(s) 76, 356
Bsp19I CCATGG 1 cut(s) 222
BspANI GGCC 2 cut(s) 221, 373
BspCNI CTCAG 2 cut(s) 366, 373
BspLI GGNNCC 1 cut(s) 240
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 238
BspTNI GGTCTC 1 cut(s) 169
BsrGI TGTACA 1 cut(s) 726
BssECI CCNNGG 3 cut(s) 51, 222, 273
BssMI GATC 2 cut(s) 76, 356
BssNAI GTATAC 1 cut(s) 229
BssT1I CCWWGG 2 cut(s) 51, 222
Bst1107I GTATAC 1 cut(s) 229
Bst2UI CCWGG 1 cut(s) 274
Bst4CI ACNGT 2 cut(s) 487, 646
BstAUI TGTACA 1 cut(s) 726
BstC8I GCNNGC 3 cut(s) 150, 303, 342
BstDEI CTNAG 2 cut(s) 353, 360
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 3 cut(s) 269, 520, 731
BstKTI GATC 2 cut(s) 79, 359
BstMAI GTCTC 2 cut(s) 106, 169
BstMBI GATC 2 cut(s) 76, 356
BstMWI GCNNNNNNNGC 1 cut(s) 247
BstNI CCWGG 1 cut(s) 274
BstNSI RCATGY 2 cut(s) 344, 727
BstPAI GACNNNNGTC 1 cut(s) 461
BstSCI CCNGG 1 cut(s) 272
BstSFI CTRYAG 1 cut(s) 303
BstSLI GKGCMC 2 cut(s) 195, 243
BstV1I GCAGC 2 cut(s) 33, 317
BstV2I GAAGAC 1 cut(s) 428
BstX2I RGATCY 1 cut(s) 356
BstXI CCANNNNNNTGG 2 cut(s) 160, 431
BstYI RGATCY 1 cut(s) 356
BstZ17I GTATAC 1 cut(s) 229
BsuRI GGCC 2 cut(s) 221, 373
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 3 cut(s) 269, 520, 731
BtsI GCAGTG 1 cut(s) 260
BtsIMutI CAGTG 2 cut(s) 99, 260
Cac8I GCNNGC 3 cut(s) 150, 303, 342
CaiI CAGNNNCTG 1 cut(s) 27
Csp6I GTAC 1 cut(s) 727
CviAII CATG 6 cut(s) 223, 235, 322, 341, 536, 724
CviQI GTAC 1 cut(s) 727
DdeI CTNAG 2 cut(s) 353, 360
DpnI GATC 2 cut(s) 78, 358
DpnII GATC 2 cut(s) 76, 356
DriI GACNNNNNGTC 1 cut(s) 432
Eam1105I GACNNNNNGTC 1 cut(s) 432
Ecl136II GAGCTC 3 cut(s) 117, 148, 328
Eco130I CCWWGG 2 cut(s) 51, 222
Eco24I GRGCYC 3 cut(s) 119, 150, 330
Eco31I GGTCTC 1 cut(s) 169
Eco32I GATATC 1 cut(s) 409
Eco53kI GAGCTC 3 cut(s) 117, 148, 328
EcoICRI GAGCTC 3 cut(s) 117, 148, 328
EcoRII CCWGG 1 cut(s) 272
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 2 cut(s) 51, 222
EcoT22I ATGCAT 1 cut(s) 508
EcoT38I GRGCYC 3 cut(s) 119, 150, 330
ErhI CCWWGG 2 cut(s) 51, 222
FaeI CATG 6 cut(s) 226, 238, 325, 344, 539, 727
FaqI GGGAC 3 cut(s) 115, 406, 469
FatI CATG 6 cut(s) 222, 234, 321, 340, 535, 723
FblI GTMKAC 2 cut(s) 228, 289
Fnu4HI GCNGC 2 cut(s) 22, 306
FokI GGATG 3 cut(s) 256, 527, 718
FriOI GRGCYC 3 cut(s) 119, 150, 330
Fsp4HI GCNGC 2 cut(s) 22, 306
FspBI CTAG 2 cut(s) 444, 558
GluI GCNGC 2 cut(s) 22, 306
GsaI CCCAGC 1 cut(s) 456
HaeIII GGCC 2 cut(s) 221, 373
Hin1II CATG 6 cut(s) 226, 238, 325, 344, 539, 727
HincII GTYRAC 2 cut(s) 290, 690
HindII GTYRAC 2 cut(s) 290, 690
HinfI GANTC 4 cut(s) 286, 294, 634, 700
Hpy166II GTNNAC 4 cut(s) 229, 290, 690, 729
Hpy188I TCNGA 5 cut(s) 285, 356, 363, 639, 705
Hpy188III TCNNGA 3 cut(s) 62, 212, 558
Hpy8I GTNNAC 4 cut(s) 229, 290, 690, 729
Hpy99I CGWCG 2 cut(s) 109, 294
HpyAV CCTTC 1 cut(s) 598
HpyCH4III ACNGT 2 cut(s) 487, 646
HpyCH4V TGCA 5 cut(s) 260, 305, 506, 631, 679
HpyF10VI GCNNNNNNNGC 1 cut(s) 247
HpyF3I CTNAG 2 cut(s) 353, 360
Hsp92II CATG 6 cut(s) 226, 238, 325, 344, 539, 727
Kzo9I GATC 2 cut(s) 76, 356
LmnI GCTCC 3 cut(s) 145, 325, 333
Lsp1109I GCAGC 2 cut(s) 33, 317
LweI GCATC 3 cut(s) 439, 493, 515
MaeI CTAG 2 cut(s) 444, 558
MaeIII GTNAC 1 cut(s) 596
MalI GATC 2 cut(s) 78, 358
MboI GATC 2 cut(s) 76, 356
MboII GAAGA 2 cut(s) 428, 469
MflI RGATCY 1 cut(s) 356
MhlI GDGCHC 5 cut(s) 119, 150, 195, 243, 330
MluCI AATT 4 cut(s) 367, 376, 567, 671
MlyI GAGTC 2 cut(s) 295, 643
MmeI TCCRAC 1 cut(s) 617
MnlI CCTC 6 cut(s) 35, 49, 148, 253, 330, 514
Mph1103I ATGCAT 1 cut(s) 508
MseI TTAA 2 cut(s) 570, 662
MspR9I CCNGG 1 cut(s) 274
MvaI CCWGG 1 cut(s) 274
MwoI GCNNNNNNNGC 1 cut(s) 247
NcoI CCATGG 1 cut(s) 222
NdeII GATC 2 cut(s) 76, 356
NlaIII CATG 6 cut(s) 226, 238, 325, 344, 539, 727
NlaIV GGNNCC 1 cut(s) 240
NmeAIII GCCGAG 1 cut(s) 98
NsiI ATGCAT 1 cut(s) 508
NspI RCATGY 2 cut(s) 344, 727
PaeI GCATGC 1 cut(s) 344
PciI ACATGT 1 cut(s) 723
PfeI GAWTC 2 cut(s) 294, 700
PflMI CCANNNNNTGG 2 cut(s) 201, 583
PkrI GCNGC 2 cut(s) 23, 307
PleI GAGTC 2 cut(s) 294, 642
PpsI GAGTC 2 cut(s) 294, 642
PscI ACATGT 1 cut(s) 723
PshAI GACNNNNGTC 1 cut(s) 461
Psp124BI GAGCTC 3 cut(s) 119, 150, 330
Psp6I CCWGG 1 cut(s) 272
PspFI CCCAGC 1 cut(s) 452
PspGI CCWGG 1 cut(s) 272
PspN4I GGNNCC 1 cut(s) 240
PstI CTGCAG 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 27
PsuI RGATCY 1 cut(s) 356
RsaI GTAC 1 cut(s) 728
RsaNI GTAC 1 cut(s) 727
SacI GAGCTC 3 cut(s) 119, 150, 330
SalI GTCGAC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 570, 662
SatI GCNGC 2 cut(s) 22, 306
Sau3AI GATC 2 cut(s) 76, 356
SchI GAGTC 2 cut(s) 295, 643
ScrFI CCNGG 1 cut(s) 274
SduI GDGCHC 5 cut(s) 119, 150, 195, 243, 330
SetI ASST 4 cut(s) 119, 150, 310, 330
SfaNI GCATC 3 cut(s) 439, 493, 515
SfcI CTRYAG 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 60
SmoI CTYRAG 1 cut(s) 60
SpeI ACTAGT 1 cut(s) 443
SphI GCATGC 1 cut(s) 344
Sse9I AATT 4 cut(s) 367, 376, 567, 671
SspMI CTAG 2 cut(s) 444, 558
SstI GAGCTC 3 cut(s) 119, 150, 330
StyD4I CCNGG 1 cut(s) 272
StyI CCWWGG 2 cut(s) 51, 222
TaaI ACNGT 2 cut(s) 487, 646
TaqI TCGA 4 cut(s) 12, 107, 213, 289
TasI AATT 4 cut(s) 367, 376, 567, 671
TatI WGTACW 1 cut(s) 726
TfiI GAWTC 2 cut(s) 294, 700
Tru1I TTAA 2 cut(s) 570, 662
Tru9I TTAA 2 cut(s) 570, 662
TscAI CASTG 2 cut(s) 99, 267
TseI GCWGC 2 cut(s) 21, 305
TspDTI ATGAA 4 cut(s) 258, 407, 463, 627
TspGWI ACGGA 1 cut(s) 158
TspRI CASTG 2 cut(s) 99, 267
Van91I CCANNNNNTGG 2 cut(s) 201, 583
XbaI TCTAGA 1 cut(s) 557
XceI RCATGY 2 cut(s) 344, 727
XmiI GTMKAC 2 cut(s) 228, 289
XspI CTAG 2 cut(s) 444, 558
Zsp2I ATGCAT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.