Rmu_co8409013.1_g000001

Mediator of RNA polymerase II transcription subunit 33A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8409013.1
OG0030033
Physical Location & Seq
Reverse (-)
2 .. 842
841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8409013.1_g000001.1.cds

Sequence Viewer

Length: 676 bp
atggctttttccgagcaaagcagcccctgggataatgtcatactggaaaccaaggaggctcaagaaaagggtagtgatcctttgctctggtcagtgcaagtctcgtcgattttgagctcggcaggggtgtccctgccctcaacagagctcgccaatgtgatggtctcatacatttgttgggaaaacaatgtgcccattttgtggaagtttctcgaaaaggccatggtatacaacatggtgccctctatgcttgttcttgcactgctttcatccagggttattccgagtcgacgattccagcctgcagcttatagactttacatggagctcctaaagagacatgctttctcactcagatcgcagataaattggcctaattatcaaatggttatgaaatcaatagatgatatccttgatttgtcccagacttttggtcttgaaactagtgatgctgggactattgtcgttgggttcatcttctcaactgtgtggcagttgattgatgcatcattagatgatgaggggttgctggaacatgatgacccagaaaagaagtctagatgggtaattaaaccccaagaaatggagatagacggttacgagaaggggaatgaacactatgagagattgcagaatcagaatactgtgatggctattgagttaatcacacagtttc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

225

Amino Acids

25.77

Weight (kDa)

4.67

Isoelectric Point (pI)

51.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030033)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0418531
rosa_multiflora Rmu_co8409013.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 238
AccB7I CCANNNNNTGG 2 cut(s) 201, 583
AccI GTMKAC 2 cut(s) 228, 289
AclWI GGATC 1 cut(s) 71
AfiI CCNNNNNNNGG 2 cut(s) 201, 583
AgsI TTSAA 1 cut(s) 440
AhdI GACNNNNNGTC 1 cut(s) 432
AhlI ACTAGT 1 cut(s) 443
AjnI CCWGG 2 cut(s) 26, 272
AluBI AGCT 4 cut(s) 117, 148, 308, 328
AluI AGCT 4 cut(s) 117, 148, 308, 328
Alw21I GWGCWC 3 cut(s) 119, 150, 330
Alw26I GTCTC 3 cut(s) 106, 169, 331
AlwI GGATC 1 cut(s) 71
AlwNI CAGNNNCTG 1 cut(s) 27
AoxI GGCC 2 cut(s) 219, 371
ApeKI GCWGC 2 cut(s) 21, 305
BaeGI GKGCMC 2 cut(s) 195, 243
BanI GGYRCC 1 cut(s) 238
BanII GRGCYC 3 cut(s) 119, 150, 330
Bbv12I GWGCWC 3 cut(s) 119, 150, 330
BbvI GCAGC 2 cut(s) 33, 317
BccI CCATC 3 cut(s) 154, 555, 643
BciT130I CCWGG 2 cut(s) 28, 274
BcoDI GTCTC 3 cut(s) 106, 169, 331
BcuI ACTAGT 1 cut(s) 443
BfaI CTAG 2 cut(s) 444, 558
BfmI CTRYAG 1 cut(s) 303
BisI GCNGC 2 cut(s) 22, 306
BlsI GCNGC 2 cut(s) 23, 307
Bme1390I CCNGG 2 cut(s) 28, 274
BmeRI GACNNNNNGTC 1 cut(s) 432
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 2 cut(s) 28, 274
BmsI GCATC 3 cut(s) 439, 493, 515
BoxI GACNNNNGTC 1 cut(s) 461
BpuEI CTTGAG 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 169
BsaJI CCNNGG 5 cut(s) 26, 27, 51, 222, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 583
Bse1I ACTGG 1 cut(s) 48
BseBI CCWGG 2 cut(s) 28, 274
BseDI CCNNGG 5 cut(s) 26, 27, 51, 222, 273
BseGI GGATG 1 cut(s) 269
BseLI CCNNNNNNNGG 2 cut(s) 201, 583
BseMII CTCAG 1 cut(s) 367
BseNI ACTGG 1 cut(s) 48
BseSI GKGCMC 2 cut(s) 195, 243
BseXI GCAGC 2 cut(s) 33, 317
BseYI CCCAGC 1 cut(s) 452
BshFI GGCC 2 cut(s) 221, 373
BshNI GGYRCC 1 cut(s) 238
BsiHKAI GWGCWC 3 cut(s) 119, 150, 330
BslFI GGGAC 3 cut(s) 115, 406, 469
BslI CCNNNNNNNGG 2 cut(s) 201, 583
BsmAI GTCTC 3 cut(s) 106, 169, 331
BsmFI GGGAC 3 cut(s) 115, 406, 469
BsnI GGCC 2 cut(s) 221, 373
Bso31I GGTCTC 1 cut(s) 169
Bsp1286I GDGCHC 5 cut(s) 119, 150, 195, 243, 330
Bsp143I GATC 2 cut(s) 76, 356
Bsp19I CCATGG 1 cut(s) 222
BspANI GGCC 2 cut(s) 221, 373
BspCNI CTCAG 1 cut(s) 366
BspLI GGNNCC 1 cut(s) 240
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 238
BspTNI GGTCTC 1 cut(s) 169
BsrI ACTGG 1 cut(s) 48
BssECI CCNNGG 5 cut(s) 26, 27, 51, 222, 273
BssMI GATC 2 cut(s) 76, 356
BssNAI GTATAC 1 cut(s) 229
BssT1I CCWWGG 2 cut(s) 51, 222
Bst1107I GTATAC 1 cut(s) 229
Bst2UI CCWGG 2 cut(s) 28, 274
Bst4CI ACNGT 4 cut(s) 487, 596, 646, 672
BstC8I GCNNGC 2 cut(s) 150, 303
BstDEI CTNAG 1 cut(s) 353
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 269
BstKTI GATC 2 cut(s) 79, 359
BstMAI GTCTC 3 cut(s) 106, 169, 331
BstMBI GATC 2 cut(s) 76, 356
BstMWI GCNNNNNNNGC 1 cut(s) 247
BstNI CCWGG 2 cut(s) 28, 274
BstNSI RCATGY 1 cut(s) 344
BstPAI GACNNNNGTC 1 cut(s) 461
BstSCI CCNGG 2 cut(s) 26, 272
BstSFI CTRYAG 1 cut(s) 303
BstSLI GKGCMC 2 cut(s) 195, 243
BstV1I GCAGC 2 cut(s) 33, 317
BstXI CCANNNNNNTGG 2 cut(s) 160, 431
BstZ17I GTATAC 1 cut(s) 229
BsuRI GGCC 2 cut(s) 221, 373
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 1 cut(s) 269
BtsI GCAGTG 1 cut(s) 260
BtsIMutI CAGTG 2 cut(s) 99, 260
Cac8I GCNNGC 2 cut(s) 150, 303
CaiI CAGNNNCTG 1 cut(s) 27
CviAII CATG 5 cut(s) 223, 235, 322, 341, 536
DdeI CTNAG 1 cut(s) 353
DpnI GATC 2 cut(s) 78, 358
DpnII GATC 2 cut(s) 76, 356
DriI GACNNNNNGTC 1 cut(s) 432
Eam1105I GACNNNNNGTC 1 cut(s) 432
Ecl136II GAGCTC 3 cut(s) 117, 148, 328
Eco130I CCWWGG 2 cut(s) 51, 222
Eco24I GRGCYC 3 cut(s) 119, 150, 330
Eco31I GGTCTC 1 cut(s) 169
Eco32I GATATC 1 cut(s) 409
Eco53kI GAGCTC 3 cut(s) 117, 148, 328
EcoICRI GAGCTC 3 cut(s) 117, 148, 328
EcoRII CCWGG 2 cut(s) 26, 272
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 2 cut(s) 51, 222
EcoT22I ATGCAT 1 cut(s) 508
EcoT38I GRGCYC 3 cut(s) 119, 150, 330
ErhI CCWWGG 2 cut(s) 51, 222
FaeI CATG 5 cut(s) 226, 238, 325, 344, 539
FaqI GGGAC 3 cut(s) 115, 406, 469
FatI CATG 5 cut(s) 222, 234, 321, 340, 535
FblI GTMKAC 2 cut(s) 228, 289
Fnu4HI GCNGC 2 cut(s) 22, 306
FokI GGATG 1 cut(s) 256
FriOI GRGCYC 3 cut(s) 119, 150, 330
Fsp4HI GCNGC 2 cut(s) 22, 306
FspBI CTAG 2 cut(s) 444, 558
GluI GCNGC 2 cut(s) 22, 306
GsaI CCCAGC 1 cut(s) 456
HaeIII GGCC 2 cut(s) 221, 373
Hin1II CATG 5 cut(s) 226, 238, 325, 344, 539
HincII GTYRAC 1 cut(s) 290
HindII GTYRAC 1 cut(s) 290
HinfI GANTC 3 cut(s) 286, 294, 634
Hpy166II GTNNAC 2 cut(s) 229, 290
Hpy188I TCNGA 4 cut(s) 13, 285, 356, 639
Hpy188III TCNNGA 4 cut(s) 62, 212, 437, 558
Hpy8I GTNNAC 2 cut(s) 229, 290
Hpy99I CGWCG 2 cut(s) 109, 294
HpyAV CCTTC 1 cut(s) 598
HpyCH4III ACNGT 4 cut(s) 487, 596, 646, 672
HpyCH4V TGCA 5 cut(s) 97, 260, 305, 506, 631
HpyF10VI GCNNNNNNNGC 1 cut(s) 247
HpyF3I CTNAG 1 cut(s) 353
Hsp92II CATG 5 cut(s) 226, 238, 325, 344, 539
Kzo9I GATC 2 cut(s) 76, 356
LmnI GCTCC 2 cut(s) 325, 333
Lsp1109I GCAGC 2 cut(s) 33, 317
LweI GCATC 3 cut(s) 439, 493, 515
MaeI CTAG 2 cut(s) 444, 558
MaeIII GTNAC 1 cut(s) 596
MalI GATC 2 cut(s) 78, 358
MboI GATC 2 cut(s) 76, 356
MboII GAAGA 1 cut(s) 469
MhlI GDGCHC 5 cut(s) 119, 150, 195, 243, 330
MluCI AATT 3 cut(s) 367, 376, 567
MlyI GAGTC 1 cut(s) 295
MnlI CCTC 4 cut(s) 49, 148, 253, 514
Mph1103I ATGCAT 1 cut(s) 508
MseI TTAA 2 cut(s) 570, 662
MspR9I CCNGG 2 cut(s) 28, 274
MvaI CCWGG 2 cut(s) 28, 274
MwoI GCNNNNNNNGC 1 cut(s) 247
NcoI CCATGG 1 cut(s) 222
NdeII GATC 2 cut(s) 76, 356
NlaIII CATG 5 cut(s) 226, 238, 325, 344, 539
NlaIV GGNNCC 1 cut(s) 240
NmeAIII GCCGAG 1 cut(s) 98
NsiI ATGCAT 1 cut(s) 508
NspI RCATGY 1 cut(s) 344
PasI CCCWGGG 1 cut(s) 27
PfeI GAWTC 2 cut(s) 294, 634
PflMI CCANNNNNTGG 2 cut(s) 201, 583
PkrI GCNGC 2 cut(s) 23, 307
PleI GAGTC 1 cut(s) 294
PpsI GAGTC 1 cut(s) 294
PshAI GACNNNNGTC 1 cut(s) 461
Psp124BI GAGCTC 3 cut(s) 119, 150, 330
Psp6I CCWGG 2 cut(s) 26, 272
PspFI CCCAGC 1 cut(s) 452
PspGI CCWGG 2 cut(s) 26, 272
PspN4I GGNNCC 1 cut(s) 240
PstI CTGCAG 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 27
SacI GAGCTC 3 cut(s) 119, 150, 330
SalI GTCGAC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 570, 662
SatI GCNGC 2 cut(s) 22, 306
Sau3AI GATC 2 cut(s) 76, 356
SchI GAGTC 1 cut(s) 295
ScrFI CCNGG 2 cut(s) 28, 274
SduI GDGCHC 5 cut(s) 119, 150, 195, 243, 330
SetI ASST 4 cut(s) 119, 150, 310, 330
SfaNI GCATC 3 cut(s) 439, 493, 515
SfcI CTRYAG 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 60
SmoI CTYRAG 1 cut(s) 60
SpeI ACTAGT 1 cut(s) 443
Sse9I AATT 3 cut(s) 367, 376, 567
SspMI CTAG 2 cut(s) 444, 558
SstI GAGCTC 3 cut(s) 119, 150, 330
StyD4I CCNGG 2 cut(s) 26, 272
StyI CCWWGG 2 cut(s) 51, 222
TaaI ACNGT 4 cut(s) 487, 596, 646, 672
TaqI TCGA 3 cut(s) 107, 213, 289
TasI AATT 3 cut(s) 367, 376, 567
TfiI GAWTC 2 cut(s) 294, 634
Tru1I TTAA 2 cut(s) 570, 662
Tru9I TTAA 2 cut(s) 570, 662
TscAI CASTG 2 cut(s) 99, 267
TseI GCWGC 2 cut(s) 21, 305
TspDTI ATGAA 4 cut(s) 258, 407, 463, 627
TspRI CASTG 2 cut(s) 99, 267
Van91I CCANNNNNTGG 2 cut(s) 201, 583
XbaI TCTAGA 1 cut(s) 557
XceI RCATGY 1 cut(s) 344
XmiI GTMKAC 2 cut(s) 228, 289
XspI CTAG 2 cut(s) 444, 558
Zsp2I ATGCAT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.