RchiOBHm_Chr4g0424561
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0022725
Physical Location & Seq
Forward (+)
50151113 .. 50152028
916 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 270 bp
ATGGAGCTCCTACATTCTTGGTCTACATGGTTGATGCTAATGACGGGGAGATTTGCGGAATCAAGAAAGGAGCTCGATGCTCTTGTCTCCGAGGAGTCACTGGAAATGGTGCCATTTCTTATATTGGGAAACCAGATTCACATACCTCAAGCTGCCTCAGAGGATGAGCTGAGTTATTCAATGGGTGTGACCAAGTACAACCTCCGCCCCCTTGAGGTGTTCATGTGCAGCATTGCCCGCAAAATGGGTTATGGCGAAGGATTTAGGTAG

Protein Analysis

89

Amino Acids

10.22

Weight (kDa)

5.05

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 16 - 88 1.4e-14 ADP-ribosylation factor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0022725)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0424561
rosa_multiflora Rmu_sc0003942.1_g000011
rosa_rugosa Rorug04G0198000
rosa_samantha Rh4CG271400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 23
AciI CCGC 3 cut(s) 56, 205, 238
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 2 cut(s) 214, 244
AgsI TTSAA 1 cut(s) 180
AluBI AGCT 4 cut(s) 7, 73, 152, 169
AluI AGCT 4 cut(s) 7, 73, 152, 169
Alw21I GWGCWC 2 cut(s) 9, 75
Alw26I GTCTC 1 cut(s) 91
ApeKI GCWGC 2 cut(s) 152, 228
BanI GGYRCC 1 cut(s) 109
BanII GRGCYC 2 cut(s) 9, 75
Bbv12I GWGCWC 2 cut(s) 9, 75
BbvI GCAGC 2 cut(s) 139, 240
BcoDI GTCTC 1 cut(s) 91
BisI GCNGC 2 cut(s) 153, 229
BlsI GCNGC 2 cut(s) 154, 230
BmiI GGNNCC 1 cut(s) 111
BmsI GCATC 2 cut(s) 24, 67
BpuEI CTTGAG 2 cut(s) 132, 233
BsaJI CCNNGG 1 cut(s) 90
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 244
Bse1I ACTGG 1 cut(s) 105
Bse3DI GCAATG 1 cut(s) 231
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 1 cut(s) 169
BseLI CCNNNNNNNGG 2 cut(s) 214, 244
BseMI GCAATG 1 cut(s) 231
BseMII CTCAG 2 cut(s) 161, 171
BseNI ACTGG 1 cut(s) 105
BseRI GAGGAG 1 cut(s) 107
BseXI GCAGC 2 cut(s) 139, 240
BsgI GTGCAG 1 cut(s) 247
BshNI GGYRCC 1 cut(s) 109
BsiHKAI GWGCWC 2 cut(s) 9, 75
BslI CCNNNNNNNGG 2 cut(s) 214, 244
BsmAI GTCTC 1 cut(s) 91
Bsp1286I GDGCHC 2 cut(s) 9, 75
BspACI CCGC 3 cut(s) 56, 205, 238
BspCNI CTCAG 2 cut(s) 162, 170
BspLI GGNNCC 1 cut(s) 111
BspT107I GGYRCC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 231
BsrI ACTGG 1 cut(s) 105
BssECI CCNNGG 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 238
BstDEI CTNAG 2 cut(s) 157, 170
BstF5I GGATG 1 cut(s) 169
BstMAI GTCTC 1 cut(s) 91
BstMWI GCNNNNNNNGC 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 139, 240
BtsCI GGATG 1 cut(s) 169
BtsIMutI CAGTG 1 cut(s) 98
Cac8I GCNNGC 1 cut(s) 238
Csp6I GTAC 1 cut(s) 196
CviAII CATG 2 cut(s) 27, 223
CviJI RGCY 4 cut(s) 7, 73, 152, 169
CviKI_1 RGCY 4 cut(s) 7, 73, 152, 169
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 2 cut(s) 157, 170
EciI GGCGGA 1 cut(s) 194
Ecl136II GAGCTC 2 cut(s) 7, 73
Eco24I GRGCYC 2 cut(s) 9, 75
Eco53kI GAGCTC 2 cut(s) 7, 73
EcoICRI GAGCTC 2 cut(s) 7, 73
EcoT38I GRGCYC 2 cut(s) 9, 75
FaeI CATG 2 cut(s) 30, 226
FaiI YATR 5 cut(s) 28, 122, 143, 224, 252
FatI CATG 2 cut(s) 26, 222
FauI CCCGC 1 cut(s) 245
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 2 cut(s) 153, 229
FokI GGATG 1 cut(s) 176
FriOI GRGCYC 2 cut(s) 9, 75
Fsp4HI GCNGC 2 cut(s) 153, 229
GluI GCNGC 2 cut(s) 153, 229
Hin1II CATG 2 cut(s) 30, 226
HinfI GANTC 3 cut(s) 59, 95, 136
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 2 cut(s) 91, 160
Hpy188III TCNNGA 1 cut(s) 63
Hpy8I GTNNAC 1 cut(s) 24
HpyAV CCTTC 1 cut(s) 251
HpyCH4V TGCA 1 cut(s) 228
HpyF10VI GCNNNNNNNGC 1 cut(s) 237
HpyF3I CTNAG 2 cut(s) 157, 170
Hsp92II CATG 2 cut(s) 30, 226
LmnI GCTCC 3 cut(s) 4, 12, 70
LpnPI CCDG 2 cut(s) 86, 146
Lsp1109I GCAGC 2 cut(s) 139, 240
LweI GCATC 2 cut(s) 24, 67
MaeIII GTNAC 2 cut(s) 96, 187
MhlI GDGCHC 2 cut(s) 9, 75
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 6 cut(s) 85, 154, 156, 166, 208, 212
MwoI GCNNNNNNNGC 1 cut(s) 237
NlaIII CATG 2 cut(s) 30, 226
NlaIV GGNNCC 1 cut(s) 111
NmuCI GTSAC 2 cut(s) 96, 187
PfeI GAWTC 2 cut(s) 59, 136
PkrI GCNGC 2 cut(s) 154, 230
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp124BI GAGCTC 2 cut(s) 9, 75
PspN4I GGNNCC 1 cut(s) 111
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SacI GAGCTC 2 cut(s) 9, 75
SatI GCNGC 2 cut(s) 153, 229
SchI GAGTC 1 cut(s) 104
SduI GDGCHC 2 cut(s) 9, 75
SetI ASST 8 cut(s) 9, 75, 148, 154, 171, 204, 219, 269
SfaNI GCATC 2 cut(s) 24, 67
SmlI CTYRAG 2 cut(s) 147, 212
SmoI CTYRAG 2 cut(s) 147, 212
SsiI CCGC 3 cut(s) 56, 205, 238
SstI GAGCTC 2 cut(s) 9, 75
TaqI TCGA 1 cut(s) 75
TatI WGTACW 1 cut(s) 195
TfiI GAWTC 2 cut(s) 59, 136
TscAI CASTG 1 cut(s) 105
TseFI GTSAC 2 cut(s) 96, 187
TseI GCWGC 2 cut(s) 152, 228
Tsp45I GTSAC 2 cut(s) 96, 187
TspDTI ATGAA 1 cut(s) 211
TspRI CASTG 1 cut(s) 105
XmiI GTMKAC 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.