RchiOBHm_Chr3g0475891

glutathione s-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0029998
Physical Location & Seq
Forward (+)
21702205 .. 21703187
983 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 621 bp
ATGGCGGACGAGGTTATTCTATTGGACGCTTATGCGAGCGTGTTTGGGATGAGGGTCAGAGTAGCTCTGGCCGAGAAGGGAATCAAATATGAGTACAGAGAAGAAGACCTGAGGAACAAGAGCCAACGGCTTCTTAAGATGAACCCGGTCCGCAAGAAGATCCCGGTTTTCATCCATAATGGCAAACCAGTCTGTGAATCGCTCGTCATTGTGCATTATATTGATGAGGTATGGAAGGATAAGGCTCCTCTGCTTCCCTCTGATCCTTACCAGAGAGCCCAGGCCAGATTCTGGGCCGATTTCATTGATAAGAAGTTATATGATGCAATCAGGAAGATATGGCCTGCAAAAGGAGAAGAGCAGGAGGCAGGAAAGAAGGAATTCATTGAAGTCCTGAAGATTTGGGTTCCTGCCTTCCTGGACATTGCTCTCATCACGTTCTACAGCTGCTTTCATGCTTGTGAGACACTTGGAAACTTCAGTATAGAGGCCGAGTGCCCCAAGCTGATTTCATGGGCCAAGAGATGCTTGCAGAAGGAGAGTGTTTCGAAGTCTCTTGCTGACCCGAAAAAGGTTTATGAGAATCTTCTTGAGATGGAGAAGAGGCTTGGTGTGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009404 GO:0009407 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016740 GO:0016765 GO:0018973 GO:0018974 GO:0019326 GO:0019439 GO:0019748 GO:0019899 GO:0031090 GO:0031668 GO:0033218 GO:0033554 GO:0034641 GO:0042221 GO:0042277 GO:0042537 GO:0042631 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043603 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046256 GO:0046260 GO:0046263 GO:0046686 GO:0048037 GO:0048046 GO:0048583 GO:0050789 GO:0050896 GO:0051186 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071944 GO:0072341 GO:0072490 GO:0072491 GO:0080167 GO:0098588 GO:0098754 GO:0098805 GO:0104004 GO:1900750 GO:1901360 GO:1901361 GO:1901564 GO:1901575 GO:1901681 GO:1901700 GO:1901701 GO:2000030
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N GST_N_3

Protein Analysis

206

Amino Acids

23.93

Weight (kDa)

8.47

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 5 - 76 6.9e-20 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 7 - 76 1.6e-16 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 76 5.5e-14 Glutathione S-transferase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029998)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0475891
rosa_samantha Rh3DG227700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 291
AciI CCGC 2 cut(s) 5, 151
AclWI GGATC 2 cut(s) 154, 257
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 1 cut(s) 380
AcuI CTGAAG 2 cut(s) 416, 463
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 3 cut(s) 291, 350, 571
AflII CTTAAG 1 cut(s) 134
AgsI TTSAA 1 cut(s) 389
AjnI CCWGG 2 cut(s) 279, 417
AluBI AGCT 3 cut(s) 65, 447, 505
AluI AGCT 3 cut(s) 65, 447, 505
Alw26I GTCTC 2 cut(s) 458, 558
AlwI GGATC 2 cut(s) 154, 257
AlwNI CAGNNNCTG 1 cut(s) 291
AoxI GGCC 6 cut(s) 69, 282, 294, 341, 489, 516
ApeKI GCWGC 1 cut(s) 447
ApoI RAATTY 1 cut(s) 380
Asp700I GAANNNNTTC 1 cut(s) 380
AspS9I GGNCC 3 cut(s) 148, 294, 516
AsuC2I CCSGG 2 cut(s) 146, 164
AsuII TTCGAA 1 cut(s) 548
AvaII GGWCC 1 cut(s) 148
AxyI CCTNAGG 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 500
BanII GRGCYC 1 cut(s) 280
BbsI GAAGAC 1 cut(s) 111
BbvI GCAGC 1 cut(s) 434
BccI CCATC 1 cut(s) 589
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 2 cut(s) 281, 419
BcnI CCSGG 2 cut(s) 146, 164
BcoDI GTCTC 2 cut(s) 458, 558
BfmI CTRYAG 1 cut(s) 442
BfrI CTTAAG 1 cut(s) 134
BisI GCNGC 1 cut(s) 448
BlsI GCNGC 1 cut(s) 449
Bme1390I CCNGG 4 cut(s) 146, 164, 281, 419
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 3 cut(s) 148, 294, 516
BmiI GGNNCC 2 cut(s) 246, 408
BmrFI CCNGG 4 cut(s) 146, 164, 281, 419
BmsI GCATC 2 cut(s) 313, 515
BpiI GAAGAC 1 cut(s) 111
Bpu14I TTCGAA 1 cut(s) 548
BpuEI CTTGAG 1 cut(s) 611
BpuMI CCSGG 2 cut(s) 146, 164
BsaJI CCNNGG 1 cut(s) 279
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 350, 571
Bse1I ACTGG 1 cut(s) 188
Bse21I CCTNAGG 1 cut(s) 110
Bse3DI GCAATG 1 cut(s) 423
BseBI CCWGG 2 cut(s) 281, 419
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 2 cut(s) 54, 171
BseLI CCNNNNNNNGG 3 cut(s) 291, 350, 571
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 1 cut(s) 101
BseNI ACTGG 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 237
BseSI GKGCMC 1 cut(s) 500
BseXI GCAGC 1 cut(s) 434
BshFI GGCC 6 cut(s) 71, 284, 296, 343, 491, 518
BsiSI CCGG 2 cut(s) 146, 164
BslI CCNNNNNNNGG 3 cut(s) 291, 350, 571
BsmAI GTCTC 2 cut(s) 458, 558
BsnI GGCC 6 cut(s) 71, 284, 296, 343, 491, 518
Bsp119I TTCGAA 1 cut(s) 548
Bsp1286I GDGCHC 2 cut(s) 280, 500
Bsp143I GATC 2 cut(s) 159, 262
BspACI CCGC 2 cut(s) 5, 151
BspANI GGCC 6 cut(s) 71, 284, 296, 343, 491, 518
BspCNI CTCAG 1 cut(s) 102
BspLI GGNNCC 2 cut(s) 246, 408
BspPI GGATC 2 cut(s) 154, 257
BspQI GCTCTTC 1 cut(s) 351
BspT104I TTCGAA 1 cut(s) 548
BspTI CTTAAG 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 423
BsrI ACTGG 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 2 cut(s) 159, 262
Bst2UI CCWGG 2 cut(s) 281, 419
Bst6I CTCTTC 2 cut(s) 351, 596
BstAFI CTTAAG 1 cut(s) 134
BstBI TTCGAA 1 cut(s) 548
BstC8I GCNNGC 3 cut(s) 37, 345, 530
BstDEI CTNAG 1 cut(s) 110
BstENI CCTNNNNNAGG 1 cut(s) 348
BstF5I GGATG 2 cut(s) 54, 171
BstKTI GATC 2 cut(s) 162, 265
BstMAI GTCTC 2 cut(s) 458, 558
BstMBI GATC 2 cut(s) 159, 262
BstNI CCWGG 2 cut(s) 281, 419
BstSCI CCNGG 4 cut(s) 144, 162, 279, 417
BstSFI CTRYAG 1 cut(s) 442
BstSLI GKGCMC 1 cut(s) 500
BstV1I GCAGC 1 cut(s) 434
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
Bsu36I CCTNAGG 1 cut(s) 110
BsuRI GGCC 6 cut(s) 71, 284, 296, 343, 491, 518
BtsCI GGATG 2 cut(s) 54, 171
Cac8I GCNNGC 3 cut(s) 37, 345, 530
CaiI CAGNNNCTG 1 cut(s) 291
Cfr13I GGNCC 3 cut(s) 148, 294, 516
CpoI CGGWCCG 1 cut(s) 148
CseI GACGC 1 cut(s) 35
Csp6I GTAC 1 cut(s) 94
CspI CGGWCCG 1 cut(s) 148
CviAII CATG 2 cut(s) 455, 513
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 1 cut(s) 110
DpnI GATC 2 cut(s) 161, 264
DpnII GATC 2 cut(s) 159, 262
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 351, 596
EarI CTCTTC 2 cut(s) 351, 596
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 2 cut(s) 416, 463
Eco81I CCTNAGG 1 cut(s) 110
EcoNI CCTNNNNNAGG 1 cut(s) 348
EcoRI GAATTC 1 cut(s) 380
EcoRII CCWGG 2 cut(s) 279, 417
EcoT38I GRGCYC 1 cut(s) 280
FaeI CATG 2 cut(s) 458, 516
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FatI CATG 2 cut(s) 454, 512
Fnu4HI GCNGC 1 cut(s) 448
FokI GGATG 2 cut(s) 61, 158
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 1 cut(s) 448
GluI GCNGC 1 cut(s) 448
HaeIII GGCC 6 cut(s) 71, 284, 296, 343, 491, 518
HapII CCGG 2 cut(s) 146, 164
HgaI GACGC 1 cut(s) 35
Hin1II CATG 2 cut(s) 458, 516
HinfI GANTC 4 cut(s) 81, 197, 288, 583
HpaII CCGG 2 cut(s) 146, 164
Hpy188I TCNGA 2 cut(s) 59, 262
Hpy188III TCNNGA 3 cut(s) 331, 394, 590
HpyAV CCTTC 5 cut(s) 70, 229, 370, 424, 529
HpyCH4IV ACGT 1 cut(s) 437
HpyCH4V TGCA 4 cut(s) 214, 326, 347, 532
HpyF3I CTNAG 1 cut(s) 110
HpySE526I ACGT 1 cut(s) 437
Hsp92II CATG 2 cut(s) 458, 516
Kzo9I GATC 2 cut(s) 159, 262
LguI GCTCTTC 1 cut(s) 351
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 1 cut(s) 434
LweI GCATC 2 cut(s) 313, 515
MaeII ACGT 1 cut(s) 437
MalI GATC 2 cut(s) 161, 264
MboI GATC 2 cut(s) 159, 262
MboII GAAGA 8 cut(s) 113, 116, 169, 346, 368, 409, 578, 613
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 2 cut(s) 280, 500
MluCI AATT 1 cut(s) 380
MnlI CCTC 9 cut(s) 4, 45, 105, 220, 258, 268, 358, 481, 597
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 1 cut(s) 135
MslI CAYNNNNRTG 1 cut(s) 459
MspA1I CMGCKG 1 cut(s) 447
MspCI CTTAAG 1 cut(s) 134
MspI CCGG 2 cut(s) 146, 164
MspR9I CCNGG 4 cut(s) 146, 164, 281, 419
MvaI CCWGG 2 cut(s) 281, 419
NciI CCSGG 2 cut(s) 146, 164
NdeII GATC 2 cut(s) 159, 262
NlaIII CATG 2 cut(s) 458, 516
NlaIV GGNNCC 2 cut(s) 246, 408
NmeAIII GCCGAG 2 cut(s) 97, 517
NspV TTCGAA 1 cut(s) 548
PciSI GCTCTTC 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 4 cut(s) 81, 197, 288, 583
PflMI CCANNNNNTGG 1 cut(s) 291
PfoI TCCNGGA 1 cut(s) 417
PkrI GCNGC 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 279, 417
PspGI CCWGG 2 cut(s) 279, 417
PspN4I GGNNCC 2 cut(s) 246, 408
PspPI GGNCC 3 cut(s) 148, 294, 516
PstNI CAGNNNCTG 1 cut(s) 291
PsuI RGATCY 1 cut(s) 159
PvuII CAGCTG 1 cut(s) 447
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 1 cut(s) 459
Rsr2I CGGWCCG 1 cut(s) 148
RsrII CGGWCCG 1 cut(s) 148
SapI GCTCTTC 1 cut(s) 351
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 1 cut(s) 448
Sau3AI GATC 2 cut(s) 159, 262
Sau96I GGNCC 3 cut(s) 148, 294, 516
ScrFI CCNGG 4 cut(s) 146, 164, 281, 419
SduI GDGCHC 2 cut(s) 280, 500
SetI ASST 8 cut(s) 15, 67, 111, 231, 440, 449, 507, 576
SfaNI GCATC 2 cut(s) 313, 515
SfcI CTRYAG 1 cut(s) 442
SfuI TTCGAA 1 cut(s) 548
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 2 cut(s) 134, 590
SmoI CTYRAG 2 cut(s) 134, 590
Sse9I AATT 1 cut(s) 380
SsiI CCGC 2 cut(s) 5, 151
StyD4I CCNGG 4 cut(s) 144, 162, 279, 417
TaiI ACGT 1 cut(s) 440
TaqI TCGA 1 cut(s) 548
TasI AATT 1 cut(s) 380
TatI WGTACW 1 cut(s) 93
TfiI GAWTC 4 cut(s) 81, 197, 288, 583
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TseI GCWGC 1 cut(s) 447
TspDTI ATGAA 6 cut(s) 155, 160, 292, 373, 443, 501
Van91I CCANNNNNTGG 1 cut(s) 291
Vha464I CTTAAG 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 348
XapI RAATTY 1 cut(s) 380
XmnI GAANNNNTTC 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.