RchiOBHm_Chr3g0492541

Histone H3.3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0026659
Physical Location & Seq
Reverse (-)
39329687 .. 39334149
4463 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 486 bp
ATGGCCCGTACGAAGCAGACTGCTCGAAAGTCGACCGGCGGCAAGGCTCCGAGGAAGCAACTGGCGACGAAGGCTGCCCGGAAGTCGGCCCCGACCACCGGAGGATCCACCGGCGGCAAGGCTCCGAGGAAGCAACTGGCGACGAAGGCTGCCCGGAAGTCGGCCCCGACGACCGGAGGAGTGAAGAAGCCGCACAGGTACCGCCCTGGAACGGTGGCTCTACGTGAAATCCGAAAGTATCAGAAGAGCACGGAGCTGCTGATCAGGAAGCTTCCGTTCCAGAGGCTTGTTCGTGAAATCGCTCAGGACTTCAAGACGGACCTGCGTTTCCAGAGCCACGCGGTGTTGGCGCTGCAGGAGGCGGCGGAGGCCTACCTTGTTGGGCTCTTTGAGGATACTAATCTGTGCGCCATTCATGCCAAGCGAGTCACAATTATGCCCAAGGATATTCAACTTGCTAGGAGAATCAGGGGTGAGAGGGCTTAA

Protein Analysis

161

Amino Acids

17.83

Weight (kDa)

11.4

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 69 - 156 1.7e-38 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026659)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0492541
rosa_roxburghii Rroxscaffold_7G00165830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 330
Acc65I GGTACC 1 cut(s) 198
AccB1I GGYRCC 1 cut(s) 198
AccI GTMKAC 1 cut(s) 32
AccII CGCG 1 cut(s) 341
AciI CCGC 7 cut(s) 39, 114, 191, 202, 341, 362, 365
AclWI GGATC 2 cut(s) 99, 112
AdeI CACNNNGTG 1 cut(s) 343
AfaI GTAC 2 cut(s) 10, 200
AfiI CCNNNNNNNGG 5 cut(s) 85, 98, 160, 173, 211
AgsI TTSAA 2 cut(s) 313, 452
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 2 cut(s) 256, 271
AluI AGCT 2 cut(s) 256, 271
Alw21I GWGCWC 1 cut(s) 251
AlwI GGATC 2 cut(s) 99, 112
AoxI GGCC 4 cut(s) 3, 87, 162, 369
ApeKI GCWGC 4 cut(s) 74, 149, 256, 352
Asp718I GGTACC 1 cut(s) 198
AspLEI GCGC 2 cut(s) 352, 410
AspS9I GGNCC 4 cut(s) 4, 88, 163, 319
AsuC2I CCSGG 2 cut(s) 79, 154
AsuHPI GGTGA 1 cut(s) 485
AvaII GGWCC 1 cut(s) 319
BamHI GGATCC 1 cut(s) 104
BanI GGYRCC 1 cut(s) 198
BanII GRGCYC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 251
BbvI GCAGC 4 cut(s) 61, 136, 243, 339
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 1 cut(s) 207
BciVI GTATCC 1 cut(s) 388
BclI TGATCA 1 cut(s) 261
BcnI CCSGG 2 cut(s) 79, 154
BfaI CTAG 1 cut(s) 459
BfmI CTRYAG 1 cut(s) 353
BfoI RGCGCY 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 330
BfuI GTATCC 1 cut(s) 388
BisI GCNGC 8 cut(s) 40, 75, 115, 150, 191, 257, 353, 363
BlsI GCNGC 8 cut(s) 41, 76, 116, 151, 192, 258, 354, 364
Bme1390I CCNGG 3 cut(s) 79, 154, 207
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 4 cut(s) 4, 88, 163, 319
BmiI GGNNCC 6 cut(s) 48, 90, 106, 123, 165, 200
BmrFI CCNGG 3 cut(s) 79, 154, 207
Bpu10I CCTNAGC 1 cut(s) 303
BpuMI CCSGG 2 cut(s) 79, 154
BsaAI YACGTR 1 cut(s) 224
BsaBI GATNNNNATC 1 cut(s) 399
BsaJI CCNNGG 4 cut(s) 50, 125, 205, 441
BsaWI WCCGGW 2 cut(s) 98, 173
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 98, 160, 173, 211
Bse118I RCCGGY 2 cut(s) 35, 110
Bse1I ACTGG 2 cut(s) 66, 141
Bse8I GATNNNNATC 1 cut(s) 399
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 4 cut(s) 50, 125, 205, 441
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 5 cut(s) 85, 98, 160, 173, 211
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 2 cut(s) 66, 141
BseRI GAGGAG 1 cut(s) 192
BseXI GCAGC 4 cut(s) 61, 136, 243, 339
Bsh1236I CGCG 1 cut(s) 341
Bsh1285I CGRYCG 2 cut(s) 36, 174
BshFI GGCC 4 cut(s) 5, 89, 164, 371
BshNI GGYRCC 1 cut(s) 198
BsiEI CGRYCG 2 cut(s) 36, 174
BsiHKAI GWGCWC 1 cut(s) 251
BsiSI CCGG 6 cut(s) 36, 79, 99, 111, 154, 174
BsiWI CGTACG 1 cut(s) 8
BslI CCNNNNNNNGG 5 cut(s) 85, 98, 160, 173, 211
BsnI GGCC 4 cut(s) 5, 89, 164, 371
Bsp1286I GDGCHC 2 cut(s) 251, 387
Bsp143I GATC 2 cut(s) 104, 261
BspACI CCGC 7 cut(s) 39, 114, 191, 202, 341, 362, 365
BspANI GGCC 4 cut(s) 5, 89, 164, 371
BspCNI CTCAG 1 cut(s) 316
BspFNI CGCG 1 cut(s) 341
BspLI GGNNCC 6 cut(s) 48, 90, 106, 123, 165, 200
BspMAI CTGCAG 1 cut(s) 357
BspMI ACCTGC 1 cut(s) 330
BspPI GGATC 2 cut(s) 99, 112
BspQI GCTCTTC 1 cut(s) 239
BspT107I GGYRCC 1 cut(s) 198
BsrFI RCCGGY 2 cut(s) 35, 110
BsrI ACTGG 2 cut(s) 66, 141
BssAI RCCGGY 2 cut(s) 35, 110
BssECI CCNNGG 4 cut(s) 50, 125, 205, 441
BssMI GATC 2 cut(s) 104, 261
BssT1I CCWWGG 1 cut(s) 441
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 214
Bst6I CTCTTC 1 cut(s) 239
BstBAI YACGTR 1 cut(s) 224
BstDEI CTNAG 1 cut(s) 303
BstFNI CGCG 1 cut(s) 341
BstH2I RGCGCY 1 cut(s) 353
BstHHI GCGC 2 cut(s) 352, 410
BstKTI GATC 2 cut(s) 107, 264
BstMBI GATC 2 cut(s) 104, 261
BstMCI CGRYCG 2 cut(s) 36, 174
BstMWI GCNNNNNNNGC 5 cut(s) 71, 146, 347, 368, 416
BstNI CCWGG 1 cut(s) 207
BstSCI CCNGG 3 cut(s) 77, 152, 205
BstSFI CTRYAG 1 cut(s) 353
BstUI CGCG 1 cut(s) 341
BstV1I GCAGC 4 cut(s) 61, 136, 243, 339
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
BsuI GTATCC 1 cut(s) 388
BsuRI GGCC 4 cut(s) 5, 89, 164, 371
BveI ACCTGC 1 cut(s) 330
CfoI GCGC 2 cut(s) 352, 410
Cfr10I RCCGGY 2 cut(s) 35, 110
Cfr13I GGNCC 4 cut(s) 4, 88, 163, 319
Csp6I GTAC 2 cut(s) 9, 199
CviAII CATG 1 cut(s) 416
CviQI GTAC 2 cut(s) 9, 199
DdeI CTNAG 1 cut(s) 303
DpnI GATC 2 cut(s) 106, 263
DpnII GATC 2 cut(s) 104, 261
DraIII CACNNNGTG 1 cut(s) 343
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
EciI GGCGGA 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 441
Eco147I AGGCCT 1 cut(s) 371
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 441
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 441
FaeI CATG 1 cut(s) 419
FaiI YATR 2 cut(s) 417, 437
FatI CATG 1 cut(s) 415
FbaI TGATCA 1 cut(s) 261
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 8 cut(s) 40, 75, 115, 150, 191, 257, 353, 363
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 8 cut(s) 40, 75, 115, 150, 191, 257, 353, 363
FspBI CTAG 1 cut(s) 459
GlaI GCGC 2 cut(s) 351, 409
GluI GCNGC 8 cut(s) 40, 75, 115, 150, 191, 257, 353, 363
HaeII RGCGCY 1 cut(s) 353
HaeIII GGCC 4 cut(s) 5, 89, 164, 371
HapII CCGG 6 cut(s) 36, 79, 99, 111, 154, 174
HhaI GCGC 2 cut(s) 352, 410
Hin1II CATG 1 cut(s) 419
Hin6I GCGC 2 cut(s) 350, 408
HinP1I GCGC 2 cut(s) 350, 408
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 2 cut(s) 426, 465
HpaII CCGG 6 cut(s) 36, 79, 99, 111, 154, 174
HphI GGTGA 1 cut(s) 485
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 4 cut(s) 51, 126, 233, 243
Hpy188III TCNNGA 6 cut(s) 265, 280, 293, 305, 313, 331
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 3 cut(s) 70, 145, 172
HpyAV CCTTC 2 cut(s) 64, 139
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 1 cut(s) 223
HpyCH4V TGCA 1 cut(s) 355
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 146, 347, 368, 416
HpyF3I CTNAG 1 cut(s) 303
HpySE526I ACGT 1 cut(s) 223
Hsp92II CATG 1 cut(s) 419
HspAI GCGC 2 cut(s) 350, 408
KpnI GGTACC 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 261
Kzo9I GATC 2 cut(s) 104, 261
LguI GCTCTTC 1 cut(s) 239
LmnI GCTCC 3 cut(s) 52, 127, 253
Lsp1109I GCAGC 4 cut(s) 61, 136, 243, 339
MaeI CTAG 1 cut(s) 459
MaeII ACGT 1 cut(s) 223
MaeIII GTNAC 1 cut(s) 427
MalI GATC 2 cut(s) 106, 263
MboI GATC 2 cut(s) 104, 261
MboII GAAGA 2 cut(s) 196, 256
MflI RGATCY 1 cut(s) 104
MhlI GDGCHC 2 cut(s) 251, 387
MluCI AATT 1 cut(s) 432
MlyI GAGTC 1 cut(s) 435
MnlI CCTC 9 cut(s) 45, 95, 120, 170, 276, 352, 361, 385, 471
MseI TTAA 1 cut(s) 484
MslI CAYNNNNRTG 1 cut(s) 434
MspI CCGG 6 cut(s) 36, 79, 99, 111, 154, 174
MspR9I CCNGG 3 cut(s) 79, 154, 207
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 341
MwoI GCNNNNNNNGC 5 cut(s) 71, 146, 347, 368, 416
NciI CCSGG 2 cut(s) 79, 154
NdeII GATC 2 cut(s) 104, 261
NlaIII CATG 1 cut(s) 419
NlaIV GGNNCC 6 cut(s) 48, 90, 106, 123, 165, 200
NmuCI GTSAC 1 cut(s) 427
PceI AGGCCT 1 cut(s) 371
PciSI GCTCTTC 1 cut(s) 239
PcsI WCGNNNNNNNCGW 2 cut(s) 167, 229
PfeI GAWTC 1 cut(s) 465
Pfl23II CGTACG 1 cut(s) 8
PkrI GCNGC 8 cut(s) 41, 76, 116, 151, 192, 258, 354, 364
PleI GAGTC 1 cut(s) 434
PpsI GAGTC 1 cut(s) 434
Ppu21I YACGTR 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspLI CGTACG 1 cut(s) 8
PspN4I GGNNCC 6 cut(s) 48, 90, 106, 123, 165, 200
PspPI GGNCC 4 cut(s) 4, 88, 163, 319
PstI CTGCAG 1 cut(s) 357
PsuI RGATCY 1 cut(s) 104
RsaI GTAC 2 cut(s) 10, 200
RsaNI GTAC 2 cut(s) 9, 199
RseI CAYNNNNRTG 1 cut(s) 434
SalI GTCGAC 1 cut(s) 31
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 1 cut(s) 484
SatI GCNGC 8 cut(s) 40, 75, 115, 150, 191, 257, 353, 363
Sau3AI GATC 2 cut(s) 104, 261
Sau96I GGNCC 4 cut(s) 4, 88, 163, 319
SchI GAGTC 1 cut(s) 435
ScrFI CCNGG 3 cut(s) 79, 154, 207
SduI GDGCHC 2 cut(s) 251, 387
SetI ASST 6 cut(s) 200, 226, 258, 273, 324, 378
SfcI CTRYAG 1 cut(s) 353
SgrAI CRCCGGYG 1 cut(s) 110
SinI GGWCC 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 434
Sse9I AATT 1 cut(s) 432
SseBI AGGCCT 1 cut(s) 371
SsiI CCGC 7 cut(s) 39, 114, 191, 202, 341, 362, 365
SspMI CTAG 1 cut(s) 459
StuI AGGCCT 1 cut(s) 371
StyD4I CCNGG 3 cut(s) 77, 152, 205
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 1 cut(s) 226
TaqI TCGA 2 cut(s) 25, 32
TasI AATT 1 cut(s) 432
TauI GCSGC 4 cut(s) 42, 117, 193, 365
TfiI GAWTC 1 cut(s) 465
Tru1I TTAA 1 cut(s) 484
Tru9I TTAA 1 cut(s) 484
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 4 cut(s) 74, 149, 256, 352
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 3 cut(s) 264, 266, 332
VpaK11BI GGWCC 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 32
XspI CTAG 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.