Rroxscaffold_7G00165830

Histone H3.3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0026659
Physical Location & Seq
Forward (+)
7461200 .. 7461778
579 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00165830.1

Sequence Viewer

Length: 405 bp
ATGCAATTCACGCGGATCAAGACCATTTCCCTTTCCGAATCGGAATTGAAGCAACTCGCCACCAAGGCTGCTCGGAAGTCAGCTCCGACCACCGGCGGAGTCAAGAAGCCCCATCGCTACCGCCCCGGAACCGTCGCCCTCCGTGAAATCCGAAAGTACCAGAAGAGCACTGAGCTTTTGATCCGCAAGCTACCATTCCAGCGCCTCGTTCGTGAAATCGCCCAGGATTTCAAGACGGACCTCAGGTTTCAGAGCCATGCTGTTCTTGCTCTTCAGGAAGCTGCTGAGGCTTACTTGGTGGGTCTGTTTGAGGACACCAATCTGTGCGCGATTCATGCCAAGAGGGTCACCATTATGCCCAAGGACATTCAACTAGCTCGGAGGATTCGGGGCGAACGTGCTTAA

Protein Analysis

134

Amino Acids

15.34

Weight (kDa)

10.52

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 42 - 129 9.8e-39 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0026659)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0492541
rosa_roxburghii Rroxscaffold_7G00165830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 13, 329
AciI CCGC 4 cut(s) 13, 96, 121, 184
AclWI GGATC 2 cut(s) 23, 175
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 1 cut(s) 92
AgsI TTSAA 3 cut(s) 49, 232, 371
AjnI CCWGG 1 cut(s) 222
AluBI AGCT 5 cut(s) 83, 175, 190, 281, 377
AluI AGCT 5 cut(s) 83, 175, 190, 281, 377
Alw21I GWGCWC 1 cut(s) 170
AlwI GGATC 2 cut(s) 23, 175
ApeKI GCWGC 2 cut(s) 68, 281
AspLEI GCGC 2 cut(s) 204, 329
AspS9I GGNCC 1 cut(s) 238
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 1 cut(s) 340
AvaII GGWCC 1 cut(s) 238
AxyI CCTNAGG 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 170
BbvCI CCTCAGC 1 cut(s) 285
BbvI GCAGC 2 cut(s) 55, 268
BccI CCATC 1 cut(s) 120
BciT130I CCWGG 1 cut(s) 224
BcnI CCSGG 1 cut(s) 126
BfaI CTAG 1 cut(s) 374
BfoI RGCGCY 1 cut(s) 205
BglI GCCNNNNNGGC 1 cut(s) 65
BisI GCNGC 2 cut(s) 69, 282
BlsI GCNGC 2 cut(s) 70, 283
Bme1390I CCNGG 2 cut(s) 126, 224
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 238
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 2 cut(s) 126, 224
Bpu10I CCTNAGC 1 cut(s) 285
BpuMI CCSGG 1 cut(s) 126
BsaJI CCNNGG 4 cut(s) 63, 124, 222, 360
Bsc4I CCNNNNNNNGG 1 cut(s) 92
Bse118I RCCGGY 1 cut(s) 92
Bse21I CCTNAGG 1 cut(s) 242
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 4 cut(s) 63, 124, 222, 360
BseLI CCNNNNNNNGG 1 cut(s) 92
BseMII CTCAG 3 cut(s) 162, 256, 276
BseXI GCAGC 2 cut(s) 55, 268
Bsh1236I CGCG 2 cut(s) 13, 329
BsiHKAI GWGCWC 1 cut(s) 170
BsiSI CCGG 2 cut(s) 93, 126
BslI CCNNNNNNNGG 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 2 cut(s) 15, 180
BspACI CCGC 4 cut(s) 13, 96, 121, 184
BspCNI CTCAG 3 cut(s) 163, 255, 277
BspFNI CGCG 2 cut(s) 13, 329
BspLI GGNNCC 1 cut(s) 130
BspPI GGATC 2 cut(s) 23, 175
BspQI GCTCTTC 2 cut(s) 158, 276
BsrFI RCCGGY 1 cut(s) 92
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 4 cut(s) 63, 124, 222, 360
BssMI GATC 2 cut(s) 15, 180
BssT1I CCWWGG 2 cut(s) 63, 360
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 2 cut(s) 158, 276
BstC8I GCNNGC 1 cut(s) 188
BstDEI CTNAG 3 cut(s) 171, 242, 285
BstEII GGTNACC 1 cut(s) 346
BstFNI CGCG 2 cut(s) 13, 329
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 2 cut(s) 204, 329
BstKTI GATC 2 cut(s) 18, 183
BstMBI GATC 2 cut(s) 15, 180
BstMWI GCNNNNNNNGC 5 cut(s) 10, 65, 266, 287, 335
BstNI CCWGG 1 cut(s) 224
BstPI GGTNACC 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 124, 222
BstUI CGCG 2 cut(s) 13, 329
BstV1I GCAGC 2 cut(s) 55, 268
Bsu36I CCTNAGG 1 cut(s) 242
BtgZI GCGATG 1 cut(s) 98
BtsIMutI CAGTG 1 cut(s) 168
Cac8I GCNNGC 1 cut(s) 188
CfoI GCGC 2 cut(s) 204, 329
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 238
Csp6I GTAC 1 cut(s) 157
CviAII CATG 2 cut(s) 257, 335
CviJI RGCY 9 cut(s) 68, 83, 109, 175, 190, 255, 281, 290, 377
CviKI_1 RGCY 9 cut(s) 68, 83, 109, 175, 190, 255, 281, 290, 377
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 3 cut(s) 171, 242, 285
DpnI GATC 2 cut(s) 17, 182
DpnII GATC 2 cut(s) 15, 180
Eam1104I CTCTTC 2 cut(s) 158, 276
EarI CTCTTC 2 cut(s) 158, 276
EciI GGCGGA 1 cut(s) 111
Eco130I CCWWGG 2 cut(s) 63, 360
Eco47I GGWCC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 257
Eco81I CCTNAGG 1 cut(s) 242
Eco91I GGTNACC 1 cut(s) 346
EcoO65I GGTNACC 1 cut(s) 346
EcoRII CCWGG 1 cut(s) 222
EcoT14I CCWWGG 2 cut(s) 63, 360
ErhI CCWWGG 2 cut(s) 63, 360
FaeI CATG 2 cut(s) 260, 338
FaiI YATR 3 cut(s) 258, 336, 356
FatI CATG 2 cut(s) 256, 334
Fnu4HI GCNGC 2 cut(s) 69, 282
Fsp4HI GCNGC 2 cut(s) 69, 282
FspBI CTAG 1 cut(s) 374
GlaI GCGC 2 cut(s) 203, 328
GluI GCNGC 2 cut(s) 69, 282
HaeII RGCGCY 1 cut(s) 205
HapII CCGG 2 cut(s) 93, 126
HhaI GCGC 2 cut(s) 204, 329
Hin1II CATG 2 cut(s) 260, 338
Hin6I GCGC 2 cut(s) 202, 327
HinP1I GCGC 2 cut(s) 202, 327
HinfI GANTC 4 cut(s) 38, 99, 331, 385
HpaII CCGG 2 cut(s) 93, 126
HphI GGTGA 1 cut(s) 340
Hpy188I TCNGA 7 cut(s) 37, 43, 75, 87, 152, 252, 381
Hpy188III TCNNGA 5 cut(s) 19, 103, 212, 232, 275
Hpy99I CGWCG 1 cut(s) 137
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 397
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 65, 266, 287, 335
HpyF3I CTNAG 3 cut(s) 171, 242, 285
HpySE526I ACGT 1 cut(s) 397
Hsp92II CATG 2 cut(s) 260, 338
HspAI GCGC 2 cut(s) 202, 327
Kzo9I GATC 2 cut(s) 15, 180
LguI GCTCTTC 2 cut(s) 158, 276
LmnI GCTCC 1 cut(s) 88
LpnPI CCDG 8 cut(s) 106, 139, 173, 209, 212, 229, 236, 260
Lsp1109I GCAGC 2 cut(s) 55, 268
MaeI CTAG 1 cut(s) 374
MaeII ACGT 1 cut(s) 397
MaeIII GTNAC 1 cut(s) 346
MalI GATC 2 cut(s) 17, 182
MboI GATC 2 cut(s) 15, 180
MboII GAAGA 2 cut(s) 175, 263
MhlI GDGCHC 1 cut(s) 170
MluCI AATT 2 cut(s) 5, 44
MlyI GAGTC 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 110
MnlI CCTC 7 cut(s) 149, 215, 251, 280, 304, 336, 375
MseI TTAA 1 cut(s) 403
MslI CAYNNNNRTG 1 cut(s) 353
MspI CCGG 2 cut(s) 93, 126
MspR9I CCNGG 2 cut(s) 126, 224
MvaI CCWGG 1 cut(s) 224
MvnI CGCG 2 cut(s) 13, 329
MwoI GCNNNNNNNGC 5 cut(s) 10, 65, 266, 287, 335
NciI CCSGG 1 cut(s) 126
NdeII GATC 2 cut(s) 15, 180
NlaIII CATG 2 cut(s) 260, 338
NlaIV GGNNCC 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 346
PciSI GCTCTTC 2 cut(s) 158, 276
PcsI WCGNNNNNNNCGW 1 cut(s) 394
PfeI GAWTC 3 cut(s) 38, 331, 385
PkrI GCNGC 2 cut(s) 70, 283
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 222
PspEI GGTNACC 1 cut(s) 346
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 1 cut(s) 238
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
RseI CAYNNNNRTG 1 cut(s) 353
SapI GCTCTTC 2 cut(s) 158, 276
SaqAI TTAA 1 cut(s) 403
SatI GCNGC 2 cut(s) 69, 282
Sau3AI GATC 2 cut(s) 15, 180
Sau96I GGNCC 1 cut(s) 238
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 2 cut(s) 126, 224
SduI GDGCHC 1 cut(s) 170
SetI ASST 8 cut(s) 85, 177, 192, 243, 248, 283, 379, 400
SgrAI CRCCGGYG 1 cut(s) 92
SinI GGWCC 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 353
Sse9I AATT 2 cut(s) 5, 44
SsiI CCGC 4 cut(s) 13, 96, 121, 184
SspMI CTAG 1 cut(s) 374
StyD4I CCNGG 2 cut(s) 124, 222
StyI CCWWGG 2 cut(s) 63, 360
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 1 cut(s) 400
TasI AATT 2 cut(s) 5, 44
TfiI GAWTC 3 cut(s) 38, 331, 385
Tru1I TTAA 1 cut(s) 403
Tru9I TTAA 1 cut(s) 403
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 2 cut(s) 68, 281
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 1 cut(s) 323
TspGWI ACGGA 2 cut(s) 131, 251
TspRI CASTG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 238
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.