RchiOBHm_Chr3g0493421

Pentatricopeptide repeat-containing protein At1g77360, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0028792
Physical Location & Seq
Reverse (-)
40592257 .. 40593422
1166 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGTATAATTTGATGATTTCTTTGCATTGCCATGGAAACAATATTAATGTAGCAAAAAGGATGCTGGATGAGATGGTCTACAATGGGACATTTCCAAATTCGCAAACATATAACATGTTGTTTAAGATGCTCATTAAGAATAGGAAGTTACAGGAGGCTGCGGTTTTGTTTACTGAGATGATTAAAAATGAGTGTGTTCTGGACCAGGATAATTGTATTGCTGCAGTCAGGATATACTTGGATTTGGGGGACCCTTATTTTGCTATAAAAGTCTGGAAGTTTATGATAGAAAATTACCGTTCTGGCTTGGAGGATACTGGGAATTTGTTGGTTCTAGGACTTCGCAATCTGAACAGAGTCCCAGAAGCAGTCAAGTATGCAGAGGATATGATGCTAAGAGGAATCAAGTTGAGTTCTTCTTCAATGTCAAAACTCAAAGACAGCCTTGTCCAAGCAAAGAAGCAATACATGTATGATGAACTTTTGAGAAAGTCGAAAGCTCAGCAGCTAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

171

Amino Acids

19.78

Weight (kDa)

9.05

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 2 - 46 1.6e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028792)

Species Orthologous Gene IDs
malus_domestica MD17G1257200.v1.1
rosa_chinensis RchiOBHm_Chr3g0493421

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 446
AccI GTMKAC 1 cut(s) 78
AciI CCGC 1 cut(s) 161
AcsI RAATTY 2 cut(s) 97, 322
AflIII ACRYGT 2 cut(s) 114, 468
AgsI TTSAA 1 cut(s) 423
AjnI CCWGG 1 cut(s) 204
AluBI AGCT 2 cut(s) 500, 508
AluI AGCT 2 cut(s) 500, 508
ApeKI GCWGC 3 cut(s) 158, 221, 505
ApoI RAATTY 2 cut(s) 97, 322
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AseI ATTAAT 1 cut(s) 45
AspS9I GGNCC 2 cut(s) 202, 250
AvaII GGWCC 2 cut(s) 202, 250
BbvI GCAGC 2 cut(s) 145, 208
BccI CCATC 1 cut(s) 67
BciT130I CCWGG 1 cut(s) 206
BciVI GTATCC 1 cut(s) 307
BfaI CTAG 2 cut(s) 335, 509
BfmI CTRYAG 1 cut(s) 222
BfuI GTATCC 1 cut(s) 307
BisI GCNGC 3 cut(s) 159, 222, 506
BlpI GCTNAGC 1 cut(s) 501
BlsI GCNGC 3 cut(s) 160, 223, 507
Bme1390I CCNGG 1 cut(s) 206
Bme18I GGWCC 2 cut(s) 202, 250
BmgT120I GGNCC 2 cut(s) 202, 250
BmiI GGNNCC 2 cut(s) 251, 252
BmrFI CCNGG 1 cut(s) 206
BmrI ACTGGG 1 cut(s) 327
BmsI GCATC 3 cut(s) 51, 117, 381
BmuI ACTGGG 1 cut(s) 327
Bpu1102I GCTNAGC 1 cut(s) 501
BsaJI CCNNGG 1 cut(s) 31
Bse1I ACTGG 1 cut(s) 322
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 31
BseGI GGATG 2 cut(s) 66, 73
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 2 cut(s) 165, 515
BseNI ACTGG 1 cut(s) 322
BseXI GCAGC 2 cut(s) 145, 208
BslFI GGGAC 3 cut(s) 100, 263, 344
BsmFI GGGAC 3 cut(s) 100, 263, 344
Bsp1720I GCTNAGC 1 cut(s) 501
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 1 cut(s) 161
BspCNI CTCAG 2 cut(s) 166, 514
BspLI GGNNCC 2 cut(s) 251, 252
BspMAI CTGCAG 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 25
BsrI ACTGG 1 cut(s) 322
BssECI CCNNGG 1 cut(s) 31
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 1 cut(s) 206
Bst4CI ACNGT 1 cut(s) 299
BstDEI CTNAG 3 cut(s) 174, 395, 501
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 2 cut(s) 66, 73
BstNI CCWGG 1 cut(s) 206
BstNSI RCATGY 2 cut(s) 118, 472
BstSCI CCNGG 1 cut(s) 204
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 2 cut(s) 145, 208
BsuI GTATCC 1 cut(s) 307
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 2 cut(s) 66, 73
Cfr13I GGNCC 2 cut(s) 202, 250
CviAII CATG 3 cut(s) 32, 115, 469
CviJI RGCY 5 cut(s) 158, 306, 444, 500, 508
CviKI_1 RGCY 5 cut(s) 158, 306, 444, 500, 508
DdeI CTNAG 3 cut(s) 174, 395, 501
DrdI GACNNNNNNGTC 1 cut(s) 446
DseDI GACNNNNNNGTC 1 cut(s) 446
Eco130I CCWWGG 1 cut(s) 31
Eco47I GGWCC 2 cut(s) 202, 250
EcoO109I RGGNCCY 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 204
EcoT14I CCWWGG 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 3 cut(s) 35, 118, 472
FalI AAGNNNNNCTT 2 cut(s) 429, 461
FaqI GGGAC 3 cut(s) 100, 263, 344
FatI CATG 3 cut(s) 31, 114, 468
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 3 cut(s) 159, 222, 506
FokI GGATG 2 cut(s) 73, 80
Fsp4HI GCNGC 3 cut(s) 159, 222, 506
FspBI CTAG 2 cut(s) 335, 509
GluI GCNGC 3 cut(s) 159, 222, 506
Hin1II CATG 3 cut(s) 35, 118, 472
HinfI GANTC 2 cut(s) 357, 402
Hpy166II GTNNAC 2 cut(s) 79, 171
Hpy188I TCNGA 1 cut(s) 351
Hpy188III TCNNGA 3 cut(s) 200, 229, 274
Hpy8I GTNNAC 2 cut(s) 79, 171
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4V TGCA 3 cut(s) 25, 224, 380
HpyF3I CTNAG 3 cut(s) 174, 395, 501
Hsp92II CATG 3 cut(s) 35, 118, 472
KflI GGGWCCC 1 cut(s) 250
Lsp1109I GCAGC 2 cut(s) 145, 208
LweI GCATC 3 cut(s) 51, 117, 381
MaeI CTAG 2 cut(s) 335, 509
MaeIII GTNAC 1 cut(s) 147
MboII GAAGA 2 cut(s) 408, 411
MluCI AATT 5 cut(s) 7, 97, 211, 292, 322
MlyI GAGTC 1 cut(s) 366
MnlI CCTC 4 cut(s) 148, 304, 376, 392
MseI TTAA 5 cut(s) 45, 123, 135, 183, 514
MslI CAYNNNNRTG 1 cut(s) 30
MspR9I CCNGG 1 cut(s) 206
MvaI CCWGG 1 cut(s) 206
NcoI CCATGG 1 cut(s) 31
NlaIII CATG 3 cut(s) 35, 118, 472
NlaIV GGNNCC 2 cut(s) 251, 252
NspI RCATGY 2 cut(s) 118, 472
PciI ACATGT 2 cut(s) 114, 468
PfeI GAWTC 1 cut(s) 402
PkrI GCNGC 3 cut(s) 160, 223, 507
PleI GAGTC 1 cut(s) 365
PpsI GAGTC 1 cut(s) 365
PpuMI RGGWCCY 1 cut(s) 250
PscI ACATGT 2 cut(s) 114, 468
PshBI ATTAAT 1 cut(s) 45
Psp5II RGGWCCY 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 204
PspGI CCWGG 1 cut(s) 204
PspN4I GGNNCC 2 cut(s) 251, 252
PspPI GGNCC 2 cut(s) 202, 250
PspPPI RGGWCCY 1 cut(s) 250
PstI CTGCAG 1 cut(s) 226
RseI CAYNNNNRTG 1 cut(s) 30
SaqAI TTAA 5 cut(s) 45, 123, 135, 183, 514
SatI GCNGC 3 cut(s) 159, 222, 506
Sau96I GGNCC 2 cut(s) 202, 250
SchI GAGTC 1 cut(s) 366
ScrFI CCNGG 1 cut(s) 206
SetI ASST 3 cut(s) 502, 510, 514
SfaNI GCATC 3 cut(s) 51, 117, 381
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 2 cut(s) 202, 250
SmiMI CAYNNNNRTG 1 cut(s) 30
Sse9I AATT 5 cut(s) 7, 97, 211, 292, 322
SsiI CCGC 1 cut(s) 161
SspI AATATT 1 cut(s) 43
SspMI CTAG 2 cut(s) 335, 509
StyD4I CCNGG 1 cut(s) 204
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 1 cut(s) 299
TaqI TCGA 1 cut(s) 494
TasI AATT 5 cut(s) 7, 97, 211, 292, 322
TfiI GAWTC 1 cut(s) 402
Tru1I TTAA 5 cut(s) 45, 123, 135, 183, 514
Tru9I TTAA 5 cut(s) 45, 123, 135, 183, 514
TseI GCWGC 3 cut(s) 158, 221, 505
TspDTI ATGAA 1 cut(s) 492
VpaK11BI GGWCC 2 cut(s) 202, 250
VspI ATTAAT 1 cut(s) 45
XapI RAATTY 2 cut(s) 97, 322
XceI RCATGY 2 cut(s) 118, 472
XmiI GTMKAC 1 cut(s) 78
XspI CTAG 2 cut(s) 335, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.