RchiOBHm_Chr2g0109511

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0029735
Physical Location & Seq
Forward (+)
20860455 .. 20861151
697 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 336 bp
ATGGCTGATGTTTTCCGTCACCCCCAGGGTTTCTACTATTTTGGTAAACTTAATGATTCACTGTCCTACGCCCTTGGAGCTGGATCCTTATTTGATGCAGCAGAATCAAATGCTGAAGCAGCAAATGTGGATCCTAGATTGGAGGCAATTGTGGAGAGAATGCTGAATAAGTGTATCATAGATGGCAAATACCAACAAGCTATGGAAAAGGCAATTGAATGTCAAAGACTGGATAAAATTGAGGAAGTAATCATAACTAGTGATTGTGTTCGAGGAACTATTGTATCAATGTCTCTCATCGGTATGTTAATCTCAGAGAATACAGATATGAGGTAA

Protein Analysis

111

Amino Acids

12.36

Weight (kDa)

4.66

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPN2_N PF21505 36 - 95 1.8e-11 26S proteasome subunit RPN2, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0029735)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0109511
rosa_samantha Rh2AG212800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 78, 91, 125, 138
AcuI CTGAAG 1 cut(s) 135
AgsI TTSAA 1 cut(s) 218
AhlI ACTAGT 1 cut(s) 257
AjnI CCWGG 1 cut(s) 24
AluBI AGCT 2 cut(s) 80, 200
AluI AGCT 2 cut(s) 80, 200
Alw26I GTCTC 1 cut(s) 297
AlwI GGATC 4 cut(s) 78, 91, 125, 138
ApeKI GCWGC 2 cut(s) 98, 119
AsuHPI GGTGA 1 cut(s) 11
BaeI ACNNNNGTAYC 2 cut(s) 267, 300
BamHI GGATCC 2 cut(s) 83, 130
BbvI GCAGC 2 cut(s) 110, 131
BccI CCATC 1 cut(s) 176
BciT130I CCWGG 1 cut(s) 26
BcoDI GTCTC 1 cut(s) 297
BcuI ACTAGT 1 cut(s) 257
BfaI CTAG 2 cut(s) 135, 258
BisI GCNGC 2 cut(s) 99, 120
BlsI GCNGC 2 cut(s) 100, 121
Bme1390I CCNGG 1 cut(s) 26
BmiI GGNNCC 2 cut(s) 85, 132
BmrFI CCNGG 1 cut(s) 26
BmsI GCATC 1 cut(s) 85
BsaJI CCNNGG 3 cut(s) 24, 25, 73
Bse1I ACTGG 1 cut(s) 234
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 3 cut(s) 24, 25, 73
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 234
BseXI GCAGC 2 cut(s) 110, 131
BsmAI GTCTC 1 cut(s) 297
BsmI GAATGC 1 cut(s) 165
Bsp143I GATC 2 cut(s) 83, 130
BspCNI CTCAG 1 cut(s) 326
BspLI GGNNCC 2 cut(s) 85, 132
BspPI GGATC 4 cut(s) 78, 91, 125, 138
BsrI ACTGG 1 cut(s) 234
BssECI CCNNGG 3 cut(s) 24, 25, 73
BssMI GATC 2 cut(s) 83, 130
BssT1I CCWWGG 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 1 cut(s) 63
BstDEI CTNAG 1 cut(s) 313
BstKTI GATC 2 cut(s) 86, 133
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 2 cut(s) 83, 130
BstMWI GCNNNNNNNGC 2 cut(s) 77, 119
BstNI CCWGG 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 24
BstV1I GCAGC 2 cut(s) 110, 131
BstX2I RGATCY 2 cut(s) 83, 130
BstYI RGATCY 2 cut(s) 83, 130
BtsIMutI CAGTG 1 cut(s) 59
CviJI RGCY 3 cut(s) 5, 80, 200
CviKI_1 RGCY 3 cut(s) 5, 80, 200
DdeI CTNAG 1 cut(s) 313
DpnI GATC 2 cut(s) 85, 132
DpnII GATC 2 cut(s) 83, 130
Eco130I CCWWGG 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 73
FaiI YATR 5 cut(s) 179, 203, 254, 305, 329
Fnu4HI GCNGC 2 cut(s) 99, 120
Fsp4HI GCNGC 2 cut(s) 99, 120
FspBI CTAG 2 cut(s) 135, 258
GluI GCNGC 2 cut(s) 99, 120
HinfI GANTC 2 cut(s) 56, 104
HphI GGTGA 1 cut(s) 11
Hpy166II GTNNAC 1 cut(s) 47
Hpy188I TCNGA 1 cut(s) 316
Hpy8I GTNNAC 1 cut(s) 47
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4V TGCA 1 cut(s) 98
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 119
HpyF3I CTNAG 1 cut(s) 313
Kzo9I GATC 2 cut(s) 83, 130
LmnI GCTCC 1 cut(s) 77
LpnPI CCDG 4 cut(s) 11, 38, 66, 215
Lsp1109I GCAGC 2 cut(s) 110, 131
LweI GCATC 1 cut(s) 85
MaeI CTAG 2 cut(s) 135, 258
MaeIII GTNAC 1 cut(s) 17
MalI GATC 2 cut(s) 85, 132
MboI GATC 2 cut(s) 83, 130
MfeI CAATTG 2 cut(s) 147, 213
MflI RGATCY 2 cut(s) 83, 130
MluCI AATT 3 cut(s) 147, 213, 237
MnlI CCTC 4 cut(s) 136, 235, 266, 324
MseI TTAA 2 cut(s) 51, 308
MslI CAYNNNNRTG 1 cut(s) 302
MspR9I CCNGG 1 cut(s) 26
MunI CAATTG 2 cut(s) 147, 213
Mva1269I GAATGC 1 cut(s) 165
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 77, 119
NdeII GATC 2 cut(s) 83, 130
NlaIV GGNNCC 2 cut(s) 85, 132
NmuCI GTSAC 1 cut(s) 17
PasI CCCWGGG 1 cut(s) 25
PctI GAATGC 1 cut(s) 165
PfeI GAWTC 2 cut(s) 56, 104
PkrI GCNGC 2 cut(s) 100, 121
Psp6I CCWGG 1 cut(s) 24
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 2 cut(s) 85, 132
PsuI RGATCY 2 cut(s) 83, 130
RseI CAYNNNNRTG 1 cut(s) 302
SaqAI TTAA 2 cut(s) 51, 308
SatI GCNGC 2 cut(s) 99, 120
Sau3AI GATC 2 cut(s) 83, 130
ScrFI CCNGG 1 cut(s) 26
SetI ASST 3 cut(s) 82, 202, 335
SfaNI GCATC 1 cut(s) 85
SgeI CNNG 9 cut(s) 37, 38, 86, 93, 147, 209, 242, 270, 284
SmiMI CAYNNNNRTG 1 cut(s) 302
SpeI ACTAGT 1 cut(s) 257
Sse9I AATT 3 cut(s) 147, 213, 237
SspMI CTAG 2 cut(s) 135, 258
StyD4I CCNGG 1 cut(s) 24
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 1 cut(s) 63
TaqI TCGA 1 cut(s) 271
TasI AATT 3 cut(s) 147, 213, 237
TfiI GAWTC 2 cut(s) 56, 104
Tru1I TTAA 2 cut(s) 51, 308
Tru9I TTAA 2 cut(s) 51, 308
TscAI CASTG 1 cut(s) 66
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 2 cut(s) 98, 119
Tsp45I GTSAC 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 5
TspRI CASTG 1 cut(s) 66
XspI CTAG 2 cut(s) 135, 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.