RchiOBHm_Chr2g0148171

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0029863
Physical Location & Seq
Forward (+)
65820204 .. 65821180
977 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 456 bp
ATGGCTACAGAGTTTCAAGAGAACCCAAGCAGTAACGGACTCGACCATCAAAAAGGCGATCATGATATCGACGGCGATGATCAGTTCAGGTACCTGATCGTACGGCCGGAGAAGGGAGGCCTCCGGGACCTGGCGAAGTACACAGTGTTGGGAGACACAAGGAGTGGGGTTCGGTTTCTGGAGGGTTCCGACGAGCAAGTGTTGGGCGACGTTGCGGAGGCGCAGAGATGGGTGATAGTGGTTTCCATCATTGCTCGGAAGATCCTAGCTCTTTTTGGGAAGCCTATGGAGTGGACTGGCTACGTGGTCGACTTCATCCTCAACCTCCTCTCTCTCAATGGAAACCTCTCTGGCTTACTCTCGCATGTTGTTCATGGTGCCGCAGAGGGGGACGGAGACTTTCATAAGTACAATTGGTCATTTAGATGGGCGGATAAATCTGTACAAGAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

151

Amino Acids

16.83

Weight (kDa)

4.99

Isoelectric Point (pI)

17.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029863)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0148171
rosa_multiflora Rmu_co8410735.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 90
AccB1I GGYRCC 2 cut(s) 90, 377
AccI GTMKAC 1 cut(s) 309
AciI CCGC 3 cut(s) 215, 381, 431
AclWI GGATC 1 cut(s) 256
AcoI YGGCCR 1 cut(s) 104
AfaI GTAC 5 cut(s) 92, 102, 140, 410, 444
AfiI CCNNNNNNNGG 3 cut(s) 113, 130, 387
AgsI TTSAA 1 cut(s) 17
AjnI CCWGG 1 cut(s) 129
AluBI AGCT 1 cut(s) 269
AluI AGCT 1 cut(s) 269
Alw26I GTCTC 2 cut(s) 147, 390
AlwI GGATC 1 cut(s) 256
AoxI GGCC 2 cut(s) 104, 118
Asp718I GGTACC 1 cut(s) 90
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 1 cut(s) 127
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 244
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 2 cut(s) 90, 377
BccI CCATC 4 cut(s) 54, 222, 254, 420
BceAI ACGGC 2 cut(s) 88, 119
BciT130I CCWGG 1 cut(s) 131
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 2 cut(s) 147, 390
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 1 cut(s) 381
BlsI GCNGC 1 cut(s) 382
Bme1390I CCNGG 2 cut(s) 125, 131
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 4 cut(s) 92, 128, 187, 379
BmrFI CCNGG 2 cut(s) 125, 131
BpmI CTGGAG 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 125
BsaAI YACGTR 1 cut(s) 304
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 130, 387
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 249
BseBI CCWGG 1 cut(s) 131
BseGI GGATG 1 cut(s) 315
BseLI CCNNNNNNNGG 3 cut(s) 113, 130, 387
BseMI GCAATG 1 cut(s) 249
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 317
BseX3I CGGCCG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 2 cut(s) 106, 120
BshNI GGYRCC 2 cut(s) 90, 377
BsiEI CGRYCG 1 cut(s) 107
BsiSI CCGG 2 cut(s) 107, 124
BsiWI CGTACG 1 cut(s) 100
BslFI GGGAC 2 cut(s) 140, 404
BslI CCNNNNNNNGG 3 cut(s) 113, 130, 387
BsmAI GTCTC 2 cut(s) 147, 390
BsmFI GGGAC 2 cut(s) 140, 404
BsnI GGCC 2 cut(s) 106, 120
Bsp1407I TGTACA 1 cut(s) 442
Bsp143I GATC 4 cut(s) 58, 79, 96, 261
BspACI CCGC 3 cut(s) 215, 381, 431
BspANI GGCC 2 cut(s) 106, 120
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 4 cut(s) 92, 128, 187, 379
BspPI GGATC 1 cut(s) 256
BspT107I GGYRCC 2 cut(s) 90, 377
BsrDI GCAATG 1 cut(s) 249
BsrGI TGTACA 1 cut(s) 442
BsrI ACTGG 1 cut(s) 301
BssMI GATC 4 cut(s) 58, 79, 96, 261
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 1 cut(s) 145
BstAUI TGTACA 1 cut(s) 442
BstBAI YACGTR 1 cut(s) 304
BstF5I GGATG 1 cut(s) 315
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 4 cut(s) 61, 82, 99, 264
BstMAI GTCTC 2 cut(s) 147, 390
BstMBI GATC 4 cut(s) 58, 79, 96, 261
BstMCI CGRYCG 1 cut(s) 107
BstNI CCWGG 1 cut(s) 131
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 123, 129
BstSFI CTRYAG 1 cut(s) 6
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BstZI CGGCCG 1 cut(s) 104
BsuRI GGCC 2 cut(s) 106, 120
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 150
CciI TCATGA 1 cut(s) 61
CfoI GCGC 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 127
Csp6I GTAC 5 cut(s) 91, 101, 139, 409, 443
CviAII CATG 3 cut(s) 62, 365, 374
CviJI RGCY 7 cut(s) 5, 106, 120, 269, 283, 300, 354
CviKI_1 RGCY 7 cut(s) 5, 106, 120, 269, 283, 300, 354
CviQI GTAC 5 cut(s) 91, 101, 139, 409, 443
DpnI GATC 4 cut(s) 60, 81, 98, 263
DpnII GATC 4 cut(s) 58, 79, 96, 261
EaeI YGGCCR 1 cut(s) 104
EagI CGGCCG 1 cut(s) 104
EciI GGCGGA 1 cut(s) 446
EclXI CGGCCG 1 cut(s) 104
Eco147I AGGCCT 1 cut(s) 120
Eco32I GATATC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 127
Eco52I CGGCCG 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 129
EcoRV GATATC 1 cut(s) 67
FaeI CATG 3 cut(s) 65, 368, 377
FaiI YATR 5 cut(s) 63, 287, 366, 375, 405
FaqI GGGAC 2 cut(s) 140, 404
FatI CATG 3 cut(s) 61, 364, 373
FbaI TGATCA 1 cut(s) 79
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 1 cut(s) 381
FokI GGATG 1 cut(s) 302
Fsp4HI GCNGC 1 cut(s) 381
FspBI CTAG 1 cut(s) 266
GlaI GCGC 1 cut(s) 222
GluI GCNGC 1 cut(s) 381
GsuI CTGGAG 1 cut(s) 200
HaeIII GGCC 2 cut(s) 106, 120
HapII CCGG 2 cut(s) 107, 124
HhaI GCGC 1 cut(s) 223
Hin1II CATG 3 cut(s) 65, 368, 377
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 1 cut(s) 39
HpaII CCGG 2 cut(s) 107, 124
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 3 cut(s) 141, 294, 310
Hpy188I TCNGA 2 cut(s) 190, 258
Hpy188III TCNNGA 3 cut(s) 17, 62, 179
Hpy8I GTNNAC 3 cut(s) 141, 294, 310
Hpy99I CGWCG 3 cut(s) 74, 194, 212
HpyAV CCTTC 2 cut(s) 106, 443
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 2 cut(s) 210, 303
HpySE526I ACGT 2 cut(s) 210, 303
Hsp92II CATG 3 cut(s) 65, 368, 377
HspAI GCGC 1 cut(s) 221
KpnI GGTACC 1 cut(s) 94
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 4 cut(s) 58, 79, 96, 261
LpnPI CCDG 9 cut(s) 73, 107, 116, 120, 137, 143, 164, 282, 336
MaeI CTAG 1 cut(s) 266
MaeII ACGT 2 cut(s) 210, 303
MaeIII GTNAC 1 cut(s) 32
MalI GATC 4 cut(s) 60, 81, 98, 263
MboI GATC 4 cut(s) 58, 79, 96, 261
MboII GAAGA 1 cut(s) 271
MfeI CAATTG 1 cut(s) 412
MflI RGATCY 1 cut(s) 261
MluCI AATT 1 cut(s) 412
MlyI GAGTC 1 cut(s) 33
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 9 cut(s) 110, 131, 175, 211, 329, 335, 338, 356, 379
MslI CAYNNNNRTG 1 cut(s) 424
MspI CCGG 2 cut(s) 107, 124
MspR9I CCNGG 2 cut(s) 125, 131
MunI CAATTG 1 cut(s) 412
MvaI CCWGG 1 cut(s) 131
NciI CCSGG 1 cut(s) 125
NdeII GATC 4 cut(s) 58, 79, 96, 261
NlaIII CATG 3 cut(s) 65, 368, 377
NlaIV GGNNCC 4 cut(s) 92, 128, 187, 379
NspI RCATGY 1 cut(s) 368
PagI TCATGA 1 cut(s) 61
PceI AGGCCT 1 cut(s) 120
Pfl23II CGTACG 1 cut(s) 100
PfoI TCCNGGA 1 cut(s) 123
PkrI GCNGC 1 cut(s) 382
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
Ppu21I YACGTR 1 cut(s) 304
PpuMI RGGWCCY 1 cut(s) 127
Psp5II RGGWCCY 1 cut(s) 127
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspLI CGTACG 1 cut(s) 100
PspN4I GGNNCC 4 cut(s) 92, 128, 187, 379
PspPI GGNCC 1 cut(s) 127
PspPPI RGGWCCY 1 cut(s) 127
PsuI RGATCY 1 cut(s) 261
RsaI GTAC 5 cut(s) 92, 102, 140, 410, 444
RsaNI GTAC 5 cut(s) 91, 101, 139, 409, 443
RseI CAYNNNNRTG 1 cut(s) 424
SalI GTCGAC 1 cut(s) 308
SatI GCNGC 1 cut(s) 381
Sau3AI GATC 4 cut(s) 58, 79, 96, 261
Sau96I GGNCC 1 cut(s) 127
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 2 cut(s) 125, 131
SetI ASST 8 cut(s) 92, 96, 132, 213, 271, 306, 327, 348
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 424
Sse9I AATT 1 cut(s) 412
SseBI AGGCCT 1 cut(s) 120
SsiI CCGC 3 cut(s) 215, 381, 431
SspMI CTAG 1 cut(s) 266
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 2 cut(s) 123, 129
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 2 cut(s) 213, 306
TaqI TCGA 3 cut(s) 42, 69, 309
TasI AATT 1 cut(s) 412
TatI WGTACW 3 cut(s) 138, 408, 442
TauI GCSGC 1 cut(s) 383
TscAI CASTG 1 cut(s) 150
TspDTI ATGAA 3 cut(s) 304, 362, 392
TspGWI ACGGA 2 cut(s) 51, 408
TspRI CASTG 1 cut(s) 150
VpaK11BI GGWCC 1 cut(s) 127
XceI RCATGY 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.