Rmu_co8410735.1_g000001

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8410735.1
OG0029863
Physical Location & Seq
Forward (+)
298 .. 681
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8410735.1_g000001.1.cds

Sequence Viewer

Length: 384 bp
atggctacagagtttcaagagaaccaaagcagtaacggactcgaccatcaaaaaggcgatcatgatatcgacggcgatgatcagtccaggtacctgatcgtacggccggagaagggaggcctccgggacctggcgaagtacacagtgttgggagacacaaggagtggggttcggtttctggagggttccgacgagcaagtgttgggcgacgttgcggaggcgcagagatgggtgatagtggtttccatcattgctcggaagatcctagctctttttgggaagcctatggagtggactggctacgtggtcgacttcatcctcaacctcctctctctcaatggaaacctctctggcttactctcgcatgttgttcatggtaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

127

Amino Acids

14.05

Weight (kDa)

5.25

Isoelectric Point (pI)

22.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029863)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0148171
rosa_multiflora Rmu_co8410735.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 90
AccB1I GGYRCC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 309
AciI CCGC 1 cut(s) 215
AclWI GGATC 1 cut(s) 256
AcoI YGGCCR 1 cut(s) 104
AfaI GTAC 3 cut(s) 92, 102, 140
AfiI CCNNNNNNNGG 2 cut(s) 113, 130
AgsI TTSAA 1 cut(s) 17
AjnI CCWGG 2 cut(s) 86, 129
AluBI AGCT 1 cut(s) 269
AluI AGCT 1 cut(s) 269
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 1 cut(s) 256
AoxI GGCC 2 cut(s) 104, 118
Asp718I GGTACC 1 cut(s) 90
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 1 cut(s) 127
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 244
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 1 cut(s) 90
BccI CCATC 3 cut(s) 54, 222, 254
BceAI ACGGC 2 cut(s) 88, 119
BciT130I CCWGG 2 cut(s) 88, 131
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 1 cut(s) 6
Bme1390I CCNGG 3 cut(s) 88, 125, 131
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 3 cut(s) 92, 128, 187
BmrFI CCNGG 3 cut(s) 88, 125, 131
BpmI CTGGAG 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 125
BsaAI YACGTR 1 cut(s) 304
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 130
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 249
BseBI CCWGG 2 cut(s) 88, 131
BseGI GGATG 1 cut(s) 315
BseLI CCNNNNNNNGG 2 cut(s) 113, 130
BseMI GCAATG 1 cut(s) 249
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 317
BseX3I CGGCCG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 2 cut(s) 106, 120
BshNI GGYRCC 1 cut(s) 90
BsiEI CGRYCG 1 cut(s) 107
BsiSI CCGG 2 cut(s) 107, 124
BsiWI CGTACG 1 cut(s) 100
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 2 cut(s) 113, 130
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 2 cut(s) 106, 120
Bsp143I GATC 4 cut(s) 58, 79, 96, 261
BspACI CCGC 1 cut(s) 215
BspANI GGCC 2 cut(s) 106, 120
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 3 cut(s) 92, 128, 187
BspPI GGATC 1 cut(s) 256
BspT107I GGYRCC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 249
BsrI ACTGG 1 cut(s) 301
BssMI GATC 4 cut(s) 58, 79, 96, 261
Bst2UI CCWGG 2 cut(s) 88, 131
Bst4CI ACNGT 1 cut(s) 145
BstBAI YACGTR 1 cut(s) 304
BstF5I GGATG 1 cut(s) 315
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 4 cut(s) 61, 82, 99, 264
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 4 cut(s) 58, 79, 96, 261
BstMCI CGRYCG 1 cut(s) 107
BstNI CCWGG 2 cut(s) 88, 131
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 3 cut(s) 86, 123, 129
BstSFI CTRYAG 1 cut(s) 6
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BstZI CGGCCG 1 cut(s) 104
BsuRI GGCC 2 cut(s) 106, 120
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 150
CciI TCATGA 1 cut(s) 61
CfoI GCGC 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 127
Csp6I GTAC 3 cut(s) 91, 101, 139
CviAII CATG 3 cut(s) 62, 365, 374
CviJI RGCY 7 cut(s) 5, 106, 120, 269, 283, 300, 354
CviKI_1 RGCY 7 cut(s) 5, 106, 120, 269, 283, 300, 354
CviQI GTAC 3 cut(s) 91, 101, 139
DpnI GATC 4 cut(s) 60, 81, 98, 263
DpnII GATC 4 cut(s) 58, 79, 96, 261
EaeI YGGCCR 1 cut(s) 104
EagI CGGCCG 1 cut(s) 104
EclXI CGGCCG 1 cut(s) 104
Eco147I AGGCCT 1 cut(s) 120
Eco32I GATATC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 127
Eco52I CGGCCG 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 127
EcoRII CCWGG 2 cut(s) 86, 129
EcoRV GATATC 1 cut(s) 67
FaeI CATG 3 cut(s) 65, 368, 377
FaiI YATR 4 cut(s) 63, 287, 366, 375
FaqI GGGAC 1 cut(s) 140
FatI CATG 3 cut(s) 61, 364, 373
FbaI TGATCA 1 cut(s) 79
FblI GTMKAC 1 cut(s) 309
FokI GGATG 1 cut(s) 302
FspBI CTAG 1 cut(s) 266
GlaI GCGC 1 cut(s) 222
GsuI CTGGAG 1 cut(s) 200
HaeIII GGCC 2 cut(s) 106, 120
HapII CCGG 2 cut(s) 107, 124
HhaI GCGC 1 cut(s) 223
Hin1II CATG 3 cut(s) 65, 368, 377
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 1 cut(s) 39
HpaII CCGG 2 cut(s) 107, 124
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 3 cut(s) 141, 294, 310
Hpy188I TCNGA 2 cut(s) 190, 258
Hpy188III TCNNGA 3 cut(s) 17, 62, 179
Hpy8I GTNNAC 3 cut(s) 141, 294, 310
Hpy99I CGWCG 3 cut(s) 74, 194, 212
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 2 cut(s) 210, 303
HpySE526I ACGT 2 cut(s) 210, 303
Hsp92II CATG 3 cut(s) 65, 368, 377
HspAI GCGC 1 cut(s) 221
KpnI GGTACC 1 cut(s) 94
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 4 cut(s) 58, 79, 96, 261
MaeI CTAG 1 cut(s) 266
MaeII ACGT 2 cut(s) 210, 303
MaeIII GTNAC 1 cut(s) 32
MalI GATC 4 cut(s) 60, 81, 98, 263
MboI GATC 4 cut(s) 58, 79, 96, 261
MboII GAAGA 1 cut(s) 271
MflI RGATCY 1 cut(s) 261
MlyI GAGTC 1 cut(s) 33
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 8 cut(s) 110, 131, 175, 211, 329, 335, 338, 356
MspI CCGG 2 cut(s) 107, 124
MspR9I CCNGG 3 cut(s) 88, 125, 131
MvaI CCWGG 2 cut(s) 88, 131
NciI CCSGG 1 cut(s) 125
NdeII GATC 4 cut(s) 58, 79, 96, 261
NlaIII CATG 3 cut(s) 65, 368, 377
NlaIV GGNNCC 3 cut(s) 92, 128, 187
NspI RCATGY 1 cut(s) 368
PagI TCATGA 1 cut(s) 61
PceI AGGCCT 1 cut(s) 120
Pfl23II CGTACG 1 cut(s) 100
PfoI TCCNGGA 1 cut(s) 123
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
Ppu21I YACGTR 1 cut(s) 304
PpuMI RGGWCCY 1 cut(s) 127
Psp5II RGGWCCY 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 86, 129
PspGI CCWGG 2 cut(s) 86, 129
PspLI CGTACG 1 cut(s) 100
PspN4I GGNNCC 3 cut(s) 92, 128, 187
PspPI GGNCC 1 cut(s) 127
PspPPI RGGWCCY 1 cut(s) 127
PsuI RGATCY 1 cut(s) 261
RsaI GTAC 3 cut(s) 92, 102, 140
RsaNI GTAC 3 cut(s) 91, 101, 139
SalI GTCGAC 1 cut(s) 308
Sau3AI GATC 4 cut(s) 58, 79, 96, 261
Sau96I GGNCC 1 cut(s) 127
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 3 cut(s) 88, 125, 131
SetI ASST 8 cut(s) 92, 96, 132, 213, 271, 306, 327, 348
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 127
SseBI AGGCCT 1 cut(s) 120
SsiI CCGC 1 cut(s) 215
SspMI CTAG 1 cut(s) 266
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 3 cut(s) 86, 123, 129
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 2 cut(s) 213, 306
TaqI TCGA 3 cut(s) 42, 69, 309
TatI WGTACW 1 cut(s) 138
TscAI CASTG 1 cut(s) 150
TspDTI ATGAA 2 cut(s) 304, 362
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 1 cut(s) 150
VpaK11BI GGWCC 1 cut(s) 127
XceI RCATGY 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.