RchiOBHm_Chr1g0362631

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0029689
Physical Location & Seq
Reverse (-)
54259981 .. 54261242
1262 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 369 bp
ATGATCAGAGACCCCTTCGATTTGACACCGGCGCCAGAGGACCCAGTTGTAGTATGTGCCATATACATGGCAGTGGCACGTGCTGTCACGCTCACATCTACCCCGGCGGCGGTGCCACCTCCACCGTTGAGGTTGGGGTTTGGTACCGTTGGTGAACGGGTCCAGGTTTTCGCCAATCACTTCCGCGTGGAGGTTGAGGAAGGTCATCTATATCACTATGATGTTTCAATTACACCCGCATGCTCGTCAAAGAAGATAAACAAAGCGGTGATCGACGAGCTGGTTTACATGTACCGTTTGAGACATGTATTTCCAGTATACGATGGCTTGAATAGTCTCTACACCACTCTGCCATATCCCTTCTCTTAG

Protein Analysis

122

Amino Acids

13.64

Weight (kDa)

6.03

Isoelectric Point (pI)

62.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 53 - 121 3.7e-15 N-terminal domain of argonaute
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0029689)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0362631
rosa_samantha Rh1CG289800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 143
AccB1I GGYRCC 3 cut(s) 31, 112, 143
AccI GTMKAC 1 cut(s) 318
AccII CGCG 1 cut(s) 186
AciI CCGC 5 cut(s) 107, 110, 184, 237, 266
AcvI CACGTG 1 cut(s) 80
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 2 cut(s) 145, 293
AfiI CCNNNNNNNGG 2 cut(s) 109, 190
AflIII ACRYGT 2 cut(s) 288, 304
AgsI TTSAA 2 cut(s) 228, 331
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 1 cut(s) 280
AluI AGCT 1 cut(s) 280
Alw26I GTCTC 3 cut(s) 3, 295, 341
Asp718I GGTACC 1 cut(s) 143
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 2 cut(s) 40, 160
AsuC2I CCSGG 1 cut(s) 104
AsuHPI GGTGA 2 cut(s) 164, 280
AvaII GGWCC 2 cut(s) 40, 160
BanI GGYRCC 3 cut(s) 31, 112, 143
BbrPI CACGTG 1 cut(s) 80
BccI CCATC 1 cut(s) 317
BciT130I CCWGG 1 cut(s) 164
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 3 cut(s) 3, 295, 341
BfoI RGCGCY 1 cut(s) 35
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 2 cut(s) 104, 164
Bme18I GGWCC 2 cut(s) 40, 160
BmgT120I GGNCC 2 cut(s) 40, 160
BmiI GGNNCC 5 cut(s) 33, 42, 114, 145, 161
BmrFI CCNGG 2 cut(s) 104, 164
BmrI ACTGGG 1 cut(s) 38
BmuI ACTGGG 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 104
BsaAI YACGTR 1 cut(s) 80
BsaHI GRCGYC 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 3
BsaJI CCNNGG 1 cut(s) 102
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 190
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 2 cut(s) 44, 314
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 1 cut(s) 102
BseLI CCNNNNNNNGG 2 cut(s) 109, 190
BseNI ACTGG 2 cut(s) 44, 314
Bsh1236I CGCG 1 cut(s) 186
BshNI GGYRCC 3 cut(s) 31, 112, 143
BsiSI CCGG 2 cut(s) 29, 104
BslI CCNNNNNNNGG 2 cut(s) 109, 190
BsmAI GTCTC 3 cut(s) 3, 295, 341
Bso31I GGTCTC 1 cut(s) 3
Bsp143I GATC 2 cut(s) 3, 270
BspACI CCGC 5 cut(s) 107, 110, 184, 237, 266
BspFNI CGCG 1 cut(s) 186
BspLI GGNNCC 5 cut(s) 33, 42, 114, 145, 161
BspT107I GGYRCC 3 cut(s) 31, 112, 143
BspTNI GGTCTC 1 cut(s) 3
BsrFI RCCGGY 1 cut(s) 28
BsrI ACTGG 2 cut(s) 44, 314
BssAI RCCGGY 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 2 cut(s) 3, 270
BssNAI GTATAC 1 cut(s) 319
BssNI GRCGYC 1 cut(s) 32
Bst1107I GTATAC 1 cut(s) 319
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 3 cut(s) 126, 148, 296
BstACI GRCGYC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 1 cut(s) 366
BstFNI CGCG 1 cut(s) 186
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 2 cut(s) 6, 273
BstMAI GTCTC 3 cut(s) 3, 295, 341
BstMBI GATC 2 cut(s) 3, 270
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 3 cut(s) 243, 292, 308
BstSCI CCNGG 2 cut(s) 102, 162
BstUI CGCG 1 cut(s) 186
BstXI CCANNNNNNTGG 1 cut(s) 67
BstZ17I GTATAC 1 cut(s) 319
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 1 cut(s) 78
Cac8I GCNNGC 1 cut(s) 241
CfoI GCGC 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 28
Cfr13I GGNCC 2 cut(s) 40, 160
Csp6I GTAC 2 cut(s) 144, 292
CviAII CATG 4 cut(s) 67, 240, 289, 305
CviJI RGCY 2 cut(s) 280, 327
CviKI_1 RGCY 2 cut(s) 280, 327
CviQI GTAC 2 cut(s) 144, 292
DdeI CTNAG 1 cut(s) 366
DinI GGCGCC 1 cut(s) 33
DpnI GATC 2 cut(s) 5, 272
DpnII GATC 2 cut(s) 3, 270
Eco31I GGTCTC 1 cut(s) 3
Eco47I GGWCC 2 cut(s) 40, 160
Eco72I CACGTG 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 162
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
FaeI CATG 4 cut(s) 70, 243, 292, 308
FatI CATG 4 cut(s) 66, 239, 288, 304
FauI CCCGC 1 cut(s) 244
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 1 cut(s) 108
Fsp4HI GCNGC 1 cut(s) 108
GlaI GCGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 108
HaeII RGCGCY 1 cut(s) 35
HapII CCGG 2 cut(s) 29, 104
HhaI GCGC 1 cut(s) 34
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 4 cut(s) 70, 243, 292, 308
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HpaII CCGG 2 cut(s) 29, 104
HphI GGTGA 2 cut(s) 164, 280
Hpy166II GTNNAC 3 cut(s) 155, 286, 319
Hpy188I TCNGA 1 cut(s) 8
Hpy8I GTNNAC 3 cut(s) 155, 286, 319
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 2 cut(s) 25, 194
HpyCH4III ACNGT 3 cut(s) 126, 148, 296
HpyCH4IV ACGT 1 cut(s) 79
HpyF3I CTNAG 1 cut(s) 366
HpySE526I ACGT 1 cut(s) 79
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 4 cut(s) 70, 243, 292, 308
HspAI GCGC 1 cut(s) 32
KasI GGCGCC 1 cut(s) 31
KpnI GGTACC 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 270
LpnPI CCDG 8 cut(s) 42, 48, 57, 117, 149, 176, 266, 327
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 1 cut(s) 85
MalI GATC 2 cut(s) 5, 272
MboI GATC 2 cut(s) 3, 270
MboII GAAGA 1 cut(s) 265
MluCI AATT 1 cut(s) 228
Mly113I GGCGCC 1 cut(s) 32
MnlI CCTC 5 cut(s) 31, 123, 129, 184, 190
MslI CAYNNNNRTG 4 cut(s) 65, 71, 219, 238
MspI CCGG 2 cut(s) 29, 104
MspR9I CCNGG 2 cut(s) 104, 164
MvaI CCWGG 1 cut(s) 164
MvnI CGCG 1 cut(s) 186
NarI GGCGCC 1 cut(s) 32
NciI CCSGG 1 cut(s) 104
NdeII GATC 2 cut(s) 3, 270
NlaIII CATG 4 cut(s) 70, 243, 292, 308
NlaIV GGNNCC 5 cut(s) 33, 42, 114, 145, 161
NmuCI GTSAC 1 cut(s) 85
NspI RCATGY 3 cut(s) 243, 292, 308
PaeI GCATGC 1 cut(s) 243
PciI ACATGT 2 cut(s) 288, 304
PkrI GCNGC 1 cut(s) 109
PluTI GGCGCC 1 cut(s) 35
PmaCI CACGTG 1 cut(s) 80
PmlI CACGTG 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 80
PpuMI RGGWCCY 1 cut(s) 40
PscI ACATGT 2 cut(s) 288, 304
Psp5II RGGWCCY 1 cut(s) 40
Psp6I CCWGG 1 cut(s) 162
PspCI CACGTG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 5 cut(s) 33, 42, 114, 145, 161
PspPI GGNCC 2 cut(s) 40, 160
PspPPI RGGWCCY 1 cut(s) 40
RsaI GTAC 2 cut(s) 145, 293
RsaNI GTAC 2 cut(s) 144, 292
RseI CAYNNNNRTG 4 cut(s) 65, 71, 219, 238
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 2 cut(s) 3, 270
Sau96I GGNCC 2 cut(s) 40, 160
ScrFI CCNGG 2 cut(s) 104, 164
SetI ASST 7 cut(s) 82, 121, 134, 168, 195, 205, 282
SfoI GGCGCC 1 cut(s) 33
SgrAI CRCCGGYG 1 cut(s) 28
SinI GGWCC 2 cut(s) 40, 160
SmiMI CAYNNNNRTG 4 cut(s) 65, 71, 219, 238
SphI GCATGC 1 cut(s) 243
Sse9I AATT 1 cut(s) 228
SsiI CCGC 5 cut(s) 107, 110, 184, 237, 266
SspDI GGCGCC 1 cut(s) 31
StyD4I CCNGG 2 cut(s) 102, 162
TaaI ACNGT 3 cut(s) 126, 148, 296
TaiI ACGT 1 cut(s) 82
TaqI TCGA 2 cut(s) 18, 273
TasI AATT 1 cut(s) 228
TauI GCSGC 1 cut(s) 110
TscAI CASTG 1 cut(s) 78
TseFI GTSAC 1 cut(s) 85
Tsp45I GTSAC 1 cut(s) 85
TspRI CASTG 1 cut(s) 78
VpaK11BI GGWCC 2 cut(s) 40, 160
XceI RCATGY 3 cut(s) 243, 292, 308
XmiI GTMKAC 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.