RchiOBHm_Chr1g0378301

PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0021316
Physical Location & Seq
Forward (+)
64891348 .. 64891767
420 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 420 bp
ATGAGCGCGTTAGGGCGTCCCCAAGATATGTTTTCAGATACCGCTATACAATTACAGCCCGTCTTTGATCAATGGATACAAAACACCCACGCTTTAGCACCCGGTGCAACGGCCCCTGGTGCAACAACAAGCACCAGTTTGACTTGGGGAGGTGGTGATTTAGTGGCAGTGGGCGGCAAAGTTGCTTTGTTACCTATTCCATTAGGAACCGCAGATTTTTTGGTACATCACATTCATGCATTTACAATTCATGTGACGGTATTGATACTCCCGAAAGGCGTTCTATTTGCTCGTAGTTCGCGTTTGATACCAGATAAAGCAAATCTTGGTTTTCGTTTTCCTTGTGATGGACCCGGAGGGGGGGACATGCCAAGTATCCGCTTGGGATCATGCCTTCTTAGGGCTATTCTGGATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PsaA_PsaB

Protein Analysis

139

Amino Acids

14.56

Weight (kDa)

6.95

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaA_PsaB PF00223 1 - 122 5.8e-44 Photosystem I psaA/psaB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021316)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0378301 RchiOBHm_MTg0498551
rosa_laevigata RLG00000000027 RLG00000000460 RLG00000000499

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 8, 301
AciI CCGC 4 cut(s) 42, 174, 210, 379
AclWI GGATC 1 cut(s) 394
AcyI GRCGYC 1 cut(s) 16
AdeI CACNNNGTG 1 cut(s) 104
AfaI GTAC 1 cut(s) 225
AfiI CCNNNNNNNGG 4 cut(s) 347, 359, 360, 400
AjnI CCWGG 1 cut(s) 115
AlwI GGATC 1 cut(s) 394
AoxI GGCC 1 cut(s) 111
AspLEI GCGC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 112, 350
AsuC2I CCSGG 2 cut(s) 102, 354
AsuHPI GGTGA 1 cut(s) 167
AvaII GGWCC 1 cut(s) 350
BccI CCATC 1 cut(s) 341
BceAI ACGGC 1 cut(s) 126
BciT130I CCWGG 1 cut(s) 117
BciVI GTATCC 2 cut(s) 69, 386
BclI TGATCA 1 cut(s) 67
BcnI CCSGG 2 cut(s) 102, 354
BfuI GTATCC 2 cut(s) 69, 386
BisI GCNGC 1 cut(s) 175
BlsI GCNGC 1 cut(s) 176
Bme1390I CCNGG 3 cut(s) 102, 117, 354
Bme18I GGWCC 1 cut(s) 350
BmgT120I GGNCC 2 cut(s) 112, 350
BmiI GGNNCC 3 cut(s) 114, 208, 352
BmrFI CCNGG 3 cut(s) 102, 117, 354
BpuMI CCSGG 2 cut(s) 102, 354
BsaHI GRCGYC 1 cut(s) 16
BsaJI CCNNGG 1 cut(s) 115
Bsc4I CCNNNNNNNGG 4 cut(s) 347, 359, 360, 400
Bse1I ACTGG 1 cut(s) 135
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 115
BseGI GGATG 1 cut(s) 418
BseLI CCNNNNNNNGG 4 cut(s) 347, 359, 360, 400
BseNI ACTGG 1 cut(s) 135
Bsh1236I CGCG 2 cut(s) 8, 301
BshFI GGCC 1 cut(s) 113
BsiSI CCGG 2 cut(s) 102, 354
BslFI GGGAC 2 cut(s) 3, 377
BslI CCNNNNNNNGG 4 cut(s) 347, 359, 360, 400
BsmFI GGGAC 2 cut(s) 3, 377
BsnI GGCC 1 cut(s) 113
Bsp143I GATC 2 cut(s) 67, 386
BspACI CCGC 4 cut(s) 42, 174, 210, 379
BspANI GGCC 1 cut(s) 113
BspFNI CGCG 2 cut(s) 8, 301
BspLI GGNNCC 3 cut(s) 114, 208, 352
BspPI GGATC 1 cut(s) 394
BsrI ACTGG 1 cut(s) 135
BssECI CCNNGG 1 cut(s) 115
BssMI GATC 2 cut(s) 67, 386
BssNI GRCGYC 1 cut(s) 16
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 259
BstACI GRCGYC 1 cut(s) 16
BstAPI GCANNNNNTGC 1 cut(s) 104
BstDEI CTNAG 1 cut(s) 398
BstF5I GGATG 1 cut(s) 418
BstFNI CGCG 2 cut(s) 8, 301
BstHHI GCGC 1 cut(s) 8
BstKTI GATC 2 cut(s) 70, 389
BstMBI GATC 2 cut(s) 67, 386
BstMWI GCNNNNNNNGC 2 cut(s) 104, 119
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 370
BstSCI CCNGG 3 cut(s) 100, 115, 352
BstUI CGCG 2 cut(s) 8, 301
BsuI GTATCC 2 cut(s) 69, 386
BsuRI GGCC 1 cut(s) 113
BtsCI GGATG 1 cut(s) 418
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 1 cut(s) 174
CfoI GCGC 1 cut(s) 8
Cfr13I GGNCC 2 cut(s) 112, 350
CseI GACGC 1 cut(s) 5
Csp6I GTAC 1 cut(s) 224
CviAII CATG 4 cut(s) 236, 251, 367, 390
CviJI RGCY 3 cut(s) 58, 113, 404
CviKI_1 RGCY 3 cut(s) 58, 113, 404
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 1 cut(s) 398
DpnI GATC 2 cut(s) 69, 388
DpnII GATC 2 cut(s) 67, 386
DraIII CACNNNGTG 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 115
EcoT22I ATGCAT 1 cut(s) 241
FaeI CATG 4 cut(s) 239, 254, 370, 393
FaiI YATR 7 cut(s) 29, 47, 237, 252, 368, 391, 418
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 2 cut(s) 3, 377
FatI CATG 4 cut(s) 235, 250, 366, 389
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 1 cut(s) 175
Fsp4HI GCNGC 1 cut(s) 175
GlaI GCGC 1 cut(s) 7
GluI GCNGC 1 cut(s) 175
HaeIII GGCC 1 cut(s) 113
HapII CCGG 2 cut(s) 102, 354
HgaI GACGC 1 cut(s) 5
HhaI GCGC 1 cut(s) 8
Hin1I GRCGYC 1 cut(s) 16
Hin1II CATG 4 cut(s) 239, 254, 370, 393
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
HpaII CCGG 2 cut(s) 102, 354
HphI GGTGA 1 cut(s) 167
Hpy188I TCNGA 1 cut(s) 37
Hpy188III TCNNGA 2 cut(s) 271, 410
HpyAV CCTTC 1 cut(s) 404
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4V TGCA 3 cut(s) 107, 122, 239
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 119
HpyF3I CTNAG 1 cut(s) 398
Hsp92I GRCGYC 1 cut(s) 16
Hsp92II CATG 4 cut(s) 239, 254, 370, 393
HspAI GCGC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 2 cut(s) 67, 386
LpnPI CCDG 7 cut(s) 102, 115, 129, 148, 324, 367, 395
MaeIII GTNAC 2 cut(s) 189, 253
MalI GATC 2 cut(s) 69, 388
MboI GATC 2 cut(s) 67, 386
MluCI AATT 2 cut(s) 50, 246
MnlI CCTC 2 cut(s) 143, 350
Mph1103I ATGCAT 1 cut(s) 241
MslI CAYNNNNRTG 1 cut(s) 234
MspI CCGG 2 cut(s) 102, 354
MspR9I CCNGG 3 cut(s) 102, 117, 354
MvaI CCWGG 1 cut(s) 117
MvnI CGCG 2 cut(s) 8, 301
MwoI GCNNNNNNNGC 2 cut(s) 104, 119
NciI CCSGG 2 cut(s) 102, 354
NdeII GATC 2 cut(s) 67, 386
NlaIII CATG 4 cut(s) 239, 254, 370, 393
NlaIV GGNNCC 3 cut(s) 114, 208, 352
NmuCI GTSAC 1 cut(s) 253
NsiI ATGCAT 1 cut(s) 241
NspI RCATGY 1 cut(s) 370
PcsI WCGNNNNNNNCGW 1 cut(s) 298
PkrI GCNGC 1 cut(s) 176
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 3 cut(s) 114, 208, 352
PspPI GGNCC 2 cut(s) 112, 350
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
RseI CAYNNNNRTG 1 cut(s) 234
SatI GCNGC 1 cut(s) 175
Sau3AI GATC 2 cut(s) 67, 386
Sau96I GGNCC 2 cut(s) 112, 350
ScrFI CCNGG 3 cut(s) 102, 117, 354
SetI ASST 2 cut(s) 154, 196
SinI GGWCC 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 234
Sse9I AATT 2 cut(s) 50, 246
SsiI CCGC 4 cut(s) 42, 174, 210, 379
StyD4I CCNGG 3 cut(s) 100, 115, 352
TaaI ACNGT 1 cut(s) 259
TasI AATT 2 cut(s) 50, 246
TauI GCSGC 1 cut(s) 177
TscAI CASTG 1 cut(s) 174
TseFI GTSAC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 2 cut(s) 224, 239
TspRI CASTG 1 cut(s) 174
VpaK11BI GGWCC 1 cut(s) 350
XceI RCATGY 1 cut(s) 370
Zsp2I ATGCAT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.