PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin
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| Species | Orthologous Gene IDs |
|---|---|
| rosa_chinensis | RchiOBHm_Chr1g0378301 RchiOBHm_MTg0498551 |
| rosa_laevigata | RLG00000000027 RLG00000000460 RLG00000000499 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 456 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 588, 702 |
| AccII | CGCG | 3 cut(s) | 8, 301, 489 |
| AciI | CCGC | 4 cut(s) | 42, 174, 210, 487 |
| AcyI | GRCGYC | 1 cut(s) | 16 |
| AdeI | CACNNNGTG | 1 cut(s) | 104 |
| AfaI | GTAC | 1 cut(s) | 225 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 347, 359, 360, 588, 702 |
| AgsI | TTSAA | 1 cut(s) | 578 |
| AjiI | CACGTC | 2 cut(s) | 554, 656 |
| AjnI | CCWGG | 1 cut(s) | 115 |
| AoxI | GGCC | 1 cut(s) | 111 |
| AseI | ATTAAT | 1 cut(s) | 474 |
| AspLEI | GCGC | 2 cut(s) | 8, 457 |
| AspS9I | GGNCC | 2 cut(s) | 112, 350 |
| AsuC2I | CCSGG | 2 cut(s) | 102, 354 |
| AsuHPI | GGTGA | 1 cut(s) | 167 |
| AvaII | GGWCC | 1 cut(s) | 350 |
| BaeGI | GKGCMC | 1 cut(s) | 505 |
| BanII | GRGCYC | 1 cut(s) | 595 |
| BccI | CCATC | 1 cut(s) | 341 |
| BceAI | ACGGC | 1 cut(s) | 126 |
| BciT130I | CCWGG | 1 cut(s) | 117 |
| BciVI | GTATCC | 1 cut(s) | 69 |
| BclI | TGATCA | 2 cut(s) | 67, 415 |
| BcnI | CCSGG | 2 cut(s) | 102, 354 |
| BfaI | CTAG | 1 cut(s) | 630 |
| BfmI | CTRYAG | 1 cut(s) | 660 |
| BfuI | GTATCC | 1 cut(s) | 69 |
| BisI | GCNGC | 1 cut(s) | 175 |
| BlsI | GCNGC | 1 cut(s) | 176 |
| Bme1390I | CCNGG | 3 cut(s) | 102, 117, 354 |
| Bme18I | GGWCC | 1 cut(s) | 350 |
| BmgBI | CACGTC | 2 cut(s) | 554, 656 |
| BmgT120I | GGNCC | 2 cut(s) | 112, 350 |
| BmiI | GGNNCC | 4 cut(s) | 114, 208, 352, 485 |
| BmrFI | CCNGG | 3 cut(s) | 102, 117, 354 |
| BmsI | GCATC | 1 cut(s) | 518 |
| BpuEI | CTTGAG | 1 cut(s) | 658 |
| BpuMI | CCSGG | 2 cut(s) | 102, 354 |
| BsaHI | GRCGYC | 1 cut(s) | 16 |
| BsaJI | CCNNGG | 2 cut(s) | 115, 649 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 347, 359, 360, 588, 702 |
| Bse1I | ACTGG | 1 cut(s) | 135 |
| BseBI | CCWGG | 1 cut(s) | 117 |
| BseDI | CCNNGG | 2 cut(s) | 115, 649 |
| BseGI | GGATG | 1 cut(s) | 509 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 347, 359, 360, 588, 702 |
| BseMII | CTCAG | 1 cut(s) | 638 |
| BseNI | ACTGG | 1 cut(s) | 135 |
| BseSI | GKGCMC | 1 cut(s) | 505 |
| Bsh1236I | CGCG | 3 cut(s) | 8, 301, 489 |
| BshFI | GGCC | 1 cut(s) | 113 |
| BsiSI | CCGG | 3 cut(s) | 102, 354, 549 |
| BslFI | GGGAC | 2 cut(s) | 3, 381 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 347, 359, 360, 588, 702 |
| BsmFI | GGGAC | 2 cut(s) | 3, 381 |
| BsnI | GGCC | 1 cut(s) | 113 |
| Bsp1286I | GDGCHC | 2 cut(s) | 505, 595 |
| Bsp143I | GATC | 2 cut(s) | 67, 415 |
| BspACI | CCGC | 4 cut(s) | 42, 174, 210, 487 |
| BspANI | GGCC | 1 cut(s) | 113 |
| BspCNI | CTCAG | 1 cut(s) | 637 |
| BspFNI | CGCG | 3 cut(s) | 8, 301, 489 |
| BspLI | GGNNCC | 4 cut(s) | 114, 208, 352, 485 |
| BsrI | ACTGG | 1 cut(s) | 135 |
| BssECI | CCNNGG | 2 cut(s) | 115, 649 |
| BssMI | GATC | 2 cut(s) | 67, 415 |
| BssNI | GRCGYC | 1 cut(s) | 16 |
| BssT1I | CCWWGG | 1 cut(s) | 649 |
| Bst2UI | CCWGG | 1 cut(s) | 117 |
| Bst4CI | ACNGT | 2 cut(s) | 259, 533 |
| BstACI | GRCGYC | 1 cut(s) | 16 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 104 |
| BstDEI | CTNAG | 2 cut(s) | 624, 715 |
| BstF5I | GGATG | 1 cut(s) | 509 |
| BstFNI | CGCG | 3 cut(s) | 8, 301, 489 |
| BstHHI | GCGC | 2 cut(s) | 8, 457 |
| BstKTI | GATC | 2 cut(s) | 70, 418 |
| BstMBI | GATC | 2 cut(s) | 67, 415 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 104, 119 |
| BstNI | CCWGG | 1 cut(s) | 117 |
| BstNSI | RCATGY | 1 cut(s) | 374 |
| BstSCI | CCNGG | 3 cut(s) | 100, 115, 352 |
| BstSFI | CTRYAG | 1 cut(s) | 660 |
| BstSLI | GKGCMC | 1 cut(s) | 505 |
| BstUI | CGCG | 3 cut(s) | 8, 301, 489 |
| BsuI | GTATCC | 1 cut(s) | 69 |
| BsuRI | GGCC | 1 cut(s) | 113 |
| BtrI | CACGTC | 2 cut(s) | 554, 656 |
| BtsCI | GGATG | 1 cut(s) | 509 |
| BtsI | GCAGTG | 1 cut(s) | 174 |
| BtsIMutI | CAGTG | 1 cut(s) | 174 |
| CfoI | GCGC | 2 cut(s) | 8, 457 |
| Cfr13I | GGNCC | 2 cut(s) | 112, 350 |
| CseI | GACGC | 1 cut(s) | 5 |
| Csp6I | GTAC | 1 cut(s) | 224 |
| CviAII | CATG | 4 cut(s) | 236, 251, 371, 702 |
| CviJI | RGCY | 6 cut(s) | 58, 113, 484, 593, 628, 635 |
| CviKI_1 | RGCY | 6 cut(s) | 58, 113, 484, 593, 628, 635 |
| CviQI | GTAC | 1 cut(s) | 224 |
| DdeI | CTNAG | 2 cut(s) | 624, 715 |
| DpnI | GATC | 2 cut(s) | 69, 417 |
| DpnII | GATC | 2 cut(s) | 67, 415 |
| DraIII | CACNNNGTG | 1 cut(s) | 104 |
| Eco130I | CCWWGG | 1 cut(s) | 649 |
| Eco24I | GRGCYC | 1 cut(s) | 595 |
| Eco47I | GGWCC | 1 cut(s) | 350 |
| EcoRII | CCWGG | 1 cut(s) | 115 |
| EcoT14I | CCWWGG | 1 cut(s) | 649 |
| EcoT22I | ATGCAT | 1 cut(s) | 241 |
| EcoT38I | GRGCYC | 1 cut(s) | 595 |
| ErhI | CCWWGG | 1 cut(s) | 649 |
| FaeI | CATG | 4 cut(s) | 239, 254, 374, 705 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 309, 341 |
| FaqI | GGGAC | 2 cut(s) | 3, 381 |
| FatI | CATG | 4 cut(s) | 235, 250, 370, 701 |
| FbaI | TGATCA | 2 cut(s) | 67, 415 |
| Fnu4HI | GCNGC | 1 cut(s) | 175 |
| FokI | GGATG | 1 cut(s) | 496 |
| FriOI | GRGCYC | 1 cut(s) | 595 |
| Fsp4HI | GCNGC | 1 cut(s) | 175 |
| FspBI | CTAG | 1 cut(s) | 630 |
| FspI | TGCGCA | 1 cut(s) | 456 |
| GlaI | GCGC | 2 cut(s) | 7, 456 |
| GluI | GCNGC | 1 cut(s) | 175 |
| HaeIII | GGCC | 1 cut(s) | 113 |
| HapII | CCGG | 3 cut(s) | 102, 354, 549 |
| HgaI | GACGC | 1 cut(s) | 5 |
| HhaI | GCGC | 2 cut(s) | 8, 457 |
| Hin1I | GRCGYC | 1 cut(s) | 16 |
| Hin1II | CATG | 4 cut(s) | 239, 254, 374, 705 |
| Hin6I | GCGC | 2 cut(s) | 6, 455 |
| HinP1I | GCGC | 2 cut(s) | 6, 455 |
| HinfI | GANTC | 1 cut(s) | 578 |
| HpaII | CCGG | 3 cut(s) | 102, 354, 549 |
| HphI | GGTGA | 1 cut(s) | 167 |
| Hpy188I | TCNGA | 2 cut(s) | 37, 394 |
| Hpy188III | TCNNGA | 1 cut(s) | 271 |
| HpyAV | CCTTC | 1 cut(s) | 689 |
| HpyCH4III | ACNGT | 2 cut(s) | 259, 533 |
| HpyCH4IV | ACGT | 2 cut(s) | 553, 655 |
| HpyCH4V | TGCA | 5 cut(s) | 107, 122, 239, 388, 731 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 104, 119 |
| HpyF3I | CTNAG | 2 cut(s) | 624, 715 |
| HpySE526I | ACGT | 2 cut(s) | 553, 655 |
| Hsp92I | GRCGYC | 1 cut(s) | 16 |
| Hsp92II | CATG | 4 cut(s) | 239, 254, 374, 705 |
| HspAI | GCGC | 2 cut(s) | 6, 455 |
| Ksp22I | TGATCA | 2 cut(s) | 67, 415 |
| Kzo9I | GATC | 2 cut(s) | 67, 415 |
| LmnI | GCTCC | 2 cut(s) | 489, 619 |
| LweI | GCATC | 1 cut(s) | 518 |
| MaeI | CTAG | 1 cut(s) | 630 |
| MaeII | ACGT | 2 cut(s) | 553, 655 |
| MaeIII | GTNAC | 4 cut(s) | 189, 253, 427, 667 |
| MalI | GATC | 2 cut(s) | 69, 417 |
| MboI | GATC | 2 cut(s) | 67, 415 |
| MboII | GAAGA | 2 cut(s) | 528, 703 |
| MfeI | CAATTG | 1 cut(s) | 376 |
| MhlI | GDGCHC | 2 cut(s) | 505, 595 |
| MluCI | AATT | 7 cut(s) | 50, 246, 376, 519, 602, 690, 723 |
| MnlI | CCTC | 3 cut(s) | 143, 350, 437 |
| Mph1103I | ATGCAT | 1 cut(s) | 241 |
| MseI | TTAA | 2 cut(s) | 474, 605 |
| MslI | CAYNNNNRTG | 1 cut(s) | 234 |
| MspI | CCGG | 3 cut(s) | 102, 354, 549 |
| MspR9I | CCNGG | 3 cut(s) | 102, 117, 354 |
| MunI | CAATTG | 1 cut(s) | 376 |
| MvaI | CCWGG | 1 cut(s) | 117 |
| MvnI | CGCG | 3 cut(s) | 8, 301, 489 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 104, 119 |
| NciI | CCSGG | 2 cut(s) | 102, 354 |
| NdeII | GATC | 2 cut(s) | 67, 415 |
| NlaIII | CATG | 4 cut(s) | 239, 254, 374, 705 |
| NlaIV | GGNNCC | 4 cut(s) | 114, 208, 352, 485 |
| NmuCI | GTSAC | 1 cut(s) | 253 |
| NsbI | TGCGCA | 1 cut(s) | 456 |
| NsiI | ATGCAT | 1 cut(s) | 241 |
| NspI | RCATGY | 1 cut(s) | 374 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 298 |
| PfeI | GAWTC | 1 cut(s) | 578 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 588, 702 |
| PkrI | GCNGC | 1 cut(s) | 176 |
| PshBI | ATTAAT | 1 cut(s) | 474 |
| Psp6I | CCWGG | 1 cut(s) | 115 |
| PspGI | CCWGG | 1 cut(s) | 115 |
| PspN4I | GGNNCC | 4 cut(s) | 114, 208, 352, 485 |
| PspPI | GGNCC | 2 cut(s) | 112, 350 |
| RsaI | GTAC | 1 cut(s) | 225 |
| RsaNI | GTAC | 1 cut(s) | 224 |
| RseI | CAYNNNNRTG | 1 cut(s) | 234 |
| SaqAI | TTAA | 2 cut(s) | 474, 605 |
| SatI | GCNGC | 1 cut(s) | 175 |
| Sau3AI | GATC | 2 cut(s) | 67, 415 |
| Sau96I | GGNCC | 2 cut(s) | 112, 350 |
| ScrFI | CCNGG | 3 cut(s) | 102, 117, 354 |
| SduI | GDGCHC | 2 cut(s) | 505, 595 |
| SetI | ASST | 5 cut(s) | 154, 196, 556, 658, 681 |
| SfaNI | GCATC | 1 cut(s) | 518 |
| SfcI | CTRYAG | 1 cut(s) | 660 |
| SinI | GGWCC | 1 cut(s) | 350 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 234 |
| SmlI | CTYRAG | 1 cut(s) | 637 |
| SmoI | CTYRAG | 1 cut(s) | 637 |
| Sse9I | AATT | 7 cut(s) | 50, 246, 376, 519, 602, 690, 723 |
| SsiI | CCGC | 4 cut(s) | 42, 174, 210, 487 |
| SspMI | CTAG | 1 cut(s) | 630 |
| StyD4I | CCNGG | 3 cut(s) | 100, 115, 352 |
| StyI | CCWWGG | 1 cut(s) | 649 |
| TaaI | ACNGT | 2 cut(s) | 259, 533 |
| TaiI | ACGT | 2 cut(s) | 556, 658 |
| TasI | AATT | 7 cut(s) | 50, 246, 376, 519, 602, 690, 723 |
| TauI | GCSGC | 1 cut(s) | 177 |
| TfiI | GAWTC | 1 cut(s) | 578 |
| Tru1I | TTAA | 2 cut(s) | 474, 605 |
| Tru9I | TTAA | 2 cut(s) | 474, 605 |
| TscAI | CASTG | 1 cut(s) | 174 |
| TseFI | GTSAC | 1 cut(s) | 253 |
| Tsp45I | GTSAC | 1 cut(s) | 253 |
| TspDTI | ATGAA | 3 cut(s) | 224, 239, 528 |
| TspRI | CASTG | 1 cut(s) | 174 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 588, 702 |
| VpaK11BI | GGWCC | 1 cut(s) | 350 |
| VspI | ATTAAT | 1 cut(s) | 474 |
| XceI | RCATGY | 1 cut(s) | 374 |
| XspI | CTAG | 1 cut(s) | 630 |
| Zsp2I | ATGCAT | 1 cut(s) | 241 |