NDH shuttles electrons from NAD(P)H plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | ATCG01090 |
| pyrus_communis | pycom16g17790 |
| rosa_chinensis | RchiOBHm_Chr5g0017191 RchiOBHm_CPg0502751 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 411 |
| AccII | CGCG | 2 cut(s) | 149, 162 |
| AciI | CCGC | 4 cut(s) | 147, 149, 160, 162 |
| AcsI | RAATTY | 2 cut(s) | 168, 179 |
| AcuI | CTGAAG | 1 cut(s) | 387 |
| AfaI | GTAC | 1 cut(s) | 62 |
| AgsI | TTSAA | 2 cut(s) | 122, 179 |
| ApeKI | GCWGC | 1 cut(s) | 53 |
| ApoI | RAATTY | 2 cut(s) | 168, 179 |
| AseI | ATTAAT | 1 cut(s) | 140 |
| AsuII | TTCGAA | 1 cut(s) | 268 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 52, 85 |
| BbvI | GCAGC | 1 cut(s) | 40 |
| BfmI | CTRYAG | 1 cut(s) | 225 |
| BglII | AGATCT | 1 cut(s) | 229 |
| BisI | GCNGC | 2 cut(s) | 54, 147 |
| BlsI | GCNGC | 2 cut(s) | 55, 148 |
| BmrI | ACTGGG | 1 cut(s) | 27 |
| BmuI | ACTGGG | 1 cut(s) | 27 |
| Bpu14I | TTCGAA | 1 cut(s) | 268 |
| BsaJI | CCNNGG | 2 cut(s) | 147, 160 |
| Bse1I | ACTGG | 1 cut(s) | 22 |
| BseDI | CCNNGG | 2 cut(s) | 147, 160 |
| BseNI | ACTGG | 1 cut(s) | 22 |
| BseXI | GCAGC | 1 cut(s) | 40 |
| Bsh1236I | CGCG | 2 cut(s) | 149, 162 |
| Bsh1285I | CGRYCG | 1 cut(s) | 166 |
| BsiEI | CGRYCG | 1 cut(s) | 166 |
| Bsp119I | TTCGAA | 1 cut(s) | 268 |
| Bsp143I | GATC | 2 cut(s) | 229, 391 |
| BspACI | CCGC | 4 cut(s) | 147, 149, 160, 162 |
| BspFNI | CGCG | 2 cut(s) | 149, 162 |
| BspHI | TCATGA | 2 cut(s) | 24, 78 |
| BspT104I | TTCGAA | 1 cut(s) | 268 |
| BsrI | ACTGG | 1 cut(s) | 22 |
| BssECI | CCNNGG | 2 cut(s) | 147, 160 |
| BssMI | GATC | 2 cut(s) | 229, 391 |
| Bst4CI | ACNGT | 1 cut(s) | 293 |
| BstBI | TTCGAA | 1 cut(s) | 268 |
| BstDSI | CCRYGG | 2 cut(s) | 147, 160 |
| BstFNI | CGCG | 2 cut(s) | 149, 162 |
| BstKTI | GATC | 2 cut(s) | 232, 394 |
| BstMBI | GATC | 2 cut(s) | 229, 391 |
| BstMCI | CGRYCG | 1 cut(s) | 166 |
| BstSFI | CTRYAG | 1 cut(s) | 225 |
| BstUI | CGCG | 2 cut(s) | 149, 162 |
| BstV1I | GCAGC | 1 cut(s) | 40 |
| BstX2I | RGATCY | 1 cut(s) | 229 |
| BstYI | RGATCY | 1 cut(s) | 229 |
| BtgI | CCRYGG | 2 cut(s) | 147, 160 |
| CciI | TCATGA | 2 cut(s) | 24, 78 |
| Cfr42I | CCGCGG | 2 cut(s) | 150, 163 |
| Csp6I | GTAC | 1 cut(s) | 61 |
| CviAII | CATG | 2 cut(s) | 25, 79 |
| CviJI | RGCY | 1 cut(s) | 53 |
| CviKI_1 | RGCY | 1 cut(s) | 53 |
| CviQI | GTAC | 1 cut(s) | 61 |
| DpnI | GATC | 2 cut(s) | 231, 393 |
| DpnII | GATC | 2 cut(s) | 229, 391 |
| Eco57I | CTGAAG | 1 cut(s) | 387 |
| EcoRI | GAATTC | 1 cut(s) | 168 |
| FaeI | CATG | 2 cut(s) | 28, 82 |
| FatI | CATG | 2 cut(s) | 24, 78 |
| Fnu4HI | GCNGC | 2 cut(s) | 54, 147 |
| Fsp4HI | GCNGC | 2 cut(s) | 54, 147 |
| GluI | GCNGC | 2 cut(s) | 54, 147 |
| Hin1II | CATG | 2 cut(s) | 28, 82 |
| HincII | GTYRAC | 1 cut(s) | 38 |
| HindII | GTYRAC | 1 cut(s) | 38 |
| HinfI | GANTC | 1 cut(s) | 306 |
| Hpy166II | GTNNAC | 3 cut(s) | 38, 107, 434 |
| Hpy188I | TCNGA | 1 cut(s) | 305 |
| Hpy188III | TCNNGA | 3 cut(s) | 25, 79, 398 |
| Hpy8I | GTNNAC | 3 cut(s) | 38, 107, 434 |
| HpyCH4III | ACNGT | 1 cut(s) | 293 |
| HpyCH4V | TGCA | 1 cut(s) | 56 |
| Hsp92II | CATG | 2 cut(s) | 28, 82 |
| KspI | CCGCGG | 2 cut(s) | 150, 163 |
| Kzo9I | GATC | 2 cut(s) | 229, 391 |
| LmnI | GCTCC | 1 cut(s) | 151 |
| LpnPI | CCDG | 1 cut(s) | 3 |
| Lsp1109I | GCAGC | 1 cut(s) | 40 |
| MaeIII | GTNAC | 3 cut(s) | 13, 112, 395 |
| MalI | GATC | 2 cut(s) | 231, 393 |
| MboI | GATC | 2 cut(s) | 229, 391 |
| MboII | GAAGA | 1 cut(s) | 380 |
| MflI | RGATCY | 1 cut(s) | 229 |
| MmeI | TCCRAC | 1 cut(s) | 368 |
| MnlI | CCTC | 1 cut(s) | 495 |
| MseI | TTAA | 4 cut(s) | 140, 285, 404, 477 |
| MspA1I | CMGCKG | 2 cut(s) | 149, 162 |
| MvnI | CGCG | 2 cut(s) | 149, 162 |
| NdeII | GATC | 2 cut(s) | 229, 391 |
| NlaIII | CATG | 2 cut(s) | 28, 82 |
| NmuCI | GTSAC | 1 cut(s) | 395 |
| NspV | TTCGAA | 1 cut(s) | 268 |
| PagI | TCATGA | 2 cut(s) | 24, 78 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 274 |
| PfeI | GAWTC | 1 cut(s) | 306 |
| PkrI | GCNGC | 2 cut(s) | 55, 148 |
| PshBI | ATTAAT | 1 cut(s) | 140 |
| PsiI | TTATAA | 1 cut(s) | 411 |
| PsuI | RGATCY | 1 cut(s) | 229 |
| RsaI | GTAC | 1 cut(s) | 62 |
| RsaNI | GTAC | 1 cut(s) | 61 |
| SacII | CCGCGG | 2 cut(s) | 150, 163 |
| SaqAI | TTAA | 4 cut(s) | 140, 285, 404, 477 |
| SatI | GCNGC | 2 cut(s) | 54, 147 |
| Sau3AI | GATC | 2 cut(s) | 229, 391 |
| SetI | ASST | 1 cut(s) | 62 |
| SfcI | CTRYAG | 1 cut(s) | 225 |
| Sfr303I | CCGCGG | 2 cut(s) | 150, 163 |
| SfuI | TTCGAA | 1 cut(s) | 268 |
| SgrBI | CCGCGG | 2 cut(s) | 150, 163 |
| SsiI | CCGC | 4 cut(s) | 147, 149, 160, 162 |
| TaaI | ACNGT | 1 cut(s) | 293 |
| TaqI | TCGA | 2 cut(s) | 166, 268 |
| TauI | GCSGC | 1 cut(s) | 149 |
| TfiI | GAWTC | 1 cut(s) | 306 |
| Tru1I | TTAA | 4 cut(s) | 140, 285, 404, 477 |
| Tru9I | TTAA | 4 cut(s) | 140, 285, 404, 477 |
| TseFI | GTSAC | 1 cut(s) | 395 |
| TseI | GCWGC | 1 cut(s) | 53 |
| Tsp45I | GTSAC | 1 cut(s) | 395 |
| TspDTI | ATGAA | 6 cut(s) | 13, 41, 67, 147, 161, 390 |
| TspGWI | ACGGA | 1 cut(s) | 275 |
| VspI | ATTAAT | 1 cut(s) | 140 |
| XapI | RAATTY | 2 cut(s) | 168, 179 |