RchiOBHm_Chr1g0313131

RING-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0024785
Physical Location & Seq
Forward (+)
246255 .. 246733
479 bp
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UTR
Exon/CDS
Intron
PRQ54395

Sequence Viewer

Length: 270 bp
ATGGCGACATTGGATTCCGACGTGCCCATGGTTCCGGCAGGCGAGGGATCGAACGATGCTGGCCCTTCTTCAGTTAAGAAGTCCAAGCGTTTCGAGATCAAGAAGTGGAATGTTGTGGCTCTCTGGGCTTGGGATATTGTTATGGACAACTGTGCTATATGCAGAAACCATATCATGGATCTTTGCATTGAGTGCCAAGCAAACCAGGCAAGCGCAACAAGCAAGAAGTGTACAGTTGCTTGGGGTATGTCTTTTGCTTGCAAACACTAA

Protein Analysis

89

Amino Acids

9.75

Weight (kDa)

7.59

Isoelectric Point (pI)

55.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 49 - 89 3.7e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024785)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0313131
rosa_samantha Rh1CG005600 Rh1DG001000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 175
AclWI GGATC 2 cut(s) 55, 186
AcuI CTGAAG 1 cut(s) 54
AfaI GTAC 1 cut(s) 232
AfiI CCNNNNNNNGG 1 cut(s) 175
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 1 cut(s) 204
AlwI GGATC 2 cut(s) 55, 186
AoxI GGCC 1 cut(s) 61
AspLEI GCGC 1 cut(s) 215
AspS9I GGNCC 1 cut(s) 62
BaeGI GKGCMC 1 cut(s) 27
BciT130I CCWGG 1 cut(s) 206
Bme1390I CCNGG 1 cut(s) 206
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 1 cut(s) 206
BmsI GCATC 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 27
BseLI CCNNNNNNNGG 1 cut(s) 175
BseSI GKGCMC 1 cut(s) 27
BshFI GGCC 1 cut(s) 63
BsiSI CCGG 1 cut(s) 35
BslI CCNNNNNNNGG 1 cut(s) 175
BsnI GGCC 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 27
Bsp1407I TGTACA 1 cut(s) 230
Bsp143I GATC 3 cut(s) 47, 96, 178
Bsp19I CCATGG 1 cut(s) 27
BspANI GGCC 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 33
BspPI GGATC 2 cut(s) 55, 186
BsrGI TGTACA 1 cut(s) 230
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 3 cut(s) 47, 96, 178
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 206
Bst4CI ACNGT 2 cut(s) 152, 235
BstAPI GCANNNNNTGC 1 cut(s) 192
BstAUI TGTACA 1 cut(s) 230
BstC8I GCNNGC 4 cut(s) 40, 61, 211, 259
BstDSI CCRYGG 1 cut(s) 27
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 3 cut(s) 50, 99, 181
BstMBI GATC 3 cut(s) 47, 96, 178
BstMWI GCNNNNNNNGC 4 cut(s) 125, 192, 206, 219
BstNI CCWGG 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 204
BstSLI GKGCMC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BsuRI GGCC 1 cut(s) 63
BtgI CCRYGG 1 cut(s) 27
BtrI CACGTC 1 cut(s) 22
Cac8I GCNNGC 4 cut(s) 40, 61, 211, 259
CfoI GCGC 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 62
Csp6I GTAC 1 cut(s) 231
CviAII CATG 2 cut(s) 28, 175
CviJI RGCY 3 cut(s) 63, 119, 128
CviKI_1 RGCY 3 cut(s) 63, 119, 128
CviQI GTAC 1 cut(s) 231
DpnI GATC 3 cut(s) 49, 98, 180
DpnII GATC 3 cut(s) 47, 96, 178
Eco130I CCWWGG 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 54
EcoRII CCWGG 1 cut(s) 204
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 2 cut(s) 31, 178
FaiI YATR 7 cut(s) 29, 143, 158, 160, 171, 176, 248
FatI CATG 2 cut(s) 27, 174
GlaI GCGC 1 cut(s) 214
HaeIII GGCC 1 cut(s) 63
HapII CCGG 1 cut(s) 35
HhaI GCGC 1 cut(s) 215
Hin1II CATG 2 cut(s) 31, 178
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HinfI GANTC 1 cut(s) 14
HpaII CCGG 1 cut(s) 35
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 2 cut(s) 94, 100
Hpy8I GTNNAC 1 cut(s) 231
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 2 cut(s) 152, 235
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 3 cut(s) 162, 186, 261
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 192, 206, 219
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 2 cut(s) 31, 178
HspAI GCGC 1 cut(s) 213
Kzo9I GATC 3 cut(s) 47, 96, 178
LpnPI CCDG 6 cut(s) 24, 45, 48, 109, 191, 218
LweI GCATC 1 cut(s) 46
MaeII ACGT 1 cut(s) 21
MalI GATC 3 cut(s) 49, 98, 180
MboI GATC 3 cut(s) 47, 96, 178
MboII GAAGA 1 cut(s) 60
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 27
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 1 cut(s) 37
MseI TTAA 1 cut(s) 75
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 206
MvaI CCWGG 1 cut(s) 206
MwoI GCNNNNNNNGC 4 cut(s) 125, 192, 206, 219
NcoI CCATGG 1 cut(s) 27
NdeII GATC 3 cut(s) 47, 96, 178
NlaIII CATG 2 cut(s) 31, 178
NlaIV GGNNCC 1 cut(s) 33
PfeI GAWTC 1 cut(s) 14
PflMI CCANNNNNTGG 1 cut(s) 175
Psp6I CCWGG 1 cut(s) 204
PspGI CCWGG 1 cut(s) 204
PspN4I GGNNCC 1 cut(s) 33
PspPI GGNCC 1 cut(s) 62
PsuI RGATCY 1 cut(s) 178
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 75
Sau3AI GATC 3 cut(s) 47, 96, 178
Sau96I GGNCC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 206
SduI GDGCHC 1 cut(s) 27
SetI ASST 1 cut(s) 24
SfaNI GCATC 1 cut(s) 46
StyD4I CCNGG 1 cut(s) 204
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 2 cut(s) 152, 235
TaiI ACGT 1 cut(s) 24
TaqI TCGA 2 cut(s) 50, 93
TatI WGTACW 1 cut(s) 230
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
Van91I CCANNNNNTGG 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.