RchiOBHm_Chr2g0107091

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0029714
Physical Location & Seq
Forward (+)
18469322 .. 18470472
1151 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48113

Sequence Viewer

Length: 858 bp
ATGGCTCAGTACATTCCAACACTGGATTACTACAGCAATAGCCTACCCAAGGCTTGCACAATGTACGCTTCTTCTGAATGCTATTTTGGCCTGAACCTTAAGCCGATGTGCGACCCTTCTGAAGTGTCATACACCATTATGCCGATGATGGGCTATTTCGAGTTCATTCCACACGATCCATCTGCTCCTCCTCTGTCCAAAAAGTCACCTCCAAGGCTCTTAGAGCTTGCTGACCTGGAAGTCGGAAAAGAATACGAGTTGGTCATTTCCACATACGCTGGACTGTGCCGTTACAGAGTCGGAGACATTCTCCAAGTCACCGGCTTCTACAACGCCGCGCCACAGTTCCGGTTTGTGAGGAGGAAGAACGTTTTGCTGAGCATTGACGCCGACAAAACCGACGAGGCGGAGCTCCAGAAAGGCATTGACAACGCCACCGAACTCCTCCGTGAATTCAACACTTCTGTGGTGGAGTACACAAGCTACGCCGACACCAAAACATTCCCAGGACATTACGTCATTTACTGGGAGTTGATGGTGAAGGACCCGTCTATCAGTGCCCCAAGCCACGAGGTTCTGAACCGGTGCTGCCTTGTCATGGAGGAGTCCCTAAACTCTGTGTACCGGCAATACCGAGTGTCTGACAAAACAATCGGGCCTCTCGAAATTAGGGTTGTGAAGAGTGGCACATTCGAGGAGCTCATGGACTACGCCATTTCCCGCGGCGCATCGATTAATCAGTACAAGGCGCCGAGGTGCGTGAACTTCTCACCCATCATGGAGTTGCTGGACTCTAGGGTTGAATCGGTGCACTTCAGCCCGGCGCTGCCACATTGGACCCCGGAGCGACGCCATTGA

Protein Analysis

285

Amino Acids

32.56

Weight (kDa)

5.47

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH3_M PF23571 44 - 121 1.4e-28 GH3 family central domain
GH3_C PF23572 137 - 256 2.8e-38 GH3 family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029714)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0107091
rosa_multiflora Rmu_co8488245.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AccB1I GGYRCC 1 cut(s) 748
AccII CGCG 2 cut(s) 338, 723
AciI CCGC 4 cut(s) 336, 407, 721, 723
AclI AACGTT 1 cut(s) 369
AclWI GGATC 1 cut(s) 170
AcsI RAATTY 1 cut(s) 452
AcuI CTGAAG 2 cut(s) 141, 799
AcyI GRCGYC 3 cut(s) 387, 749, 850
AfaI GTAC 5 cut(s) 11, 65, 476, 623, 743
AfiI CCNNNNNNNGG 3 cut(s) 49, 149, 598
AflII CTTAAG 1 cut(s) 98
AgeI ACCGGT 1 cut(s) 582
AgsI TTSAA 2 cut(s) 457, 803
AhdI GACNNNNNGTC 1 cut(s) 515
AjnI CCWGG 2 cut(s) 234, 505
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AleI CACNNNNGTG 1 cut(s) 464
AluBI AGCT 4 cut(s) 226, 412, 483, 700
AluI AGCT 4 cut(s) 226, 412, 483, 700
Alw21I GWGCWC 3 cut(s) 414, 702, 813
Alw26I GTCTC 1 cut(s) 297
Alw44I GTGCAC 1 cut(s) 809
AlwI GGATC 1 cut(s) 170
AoxI GGCC 2 cut(s) 88, 656
ApaLI GTGCAC 1 cut(s) 809
ApeKI GCWGC 2 cut(s) 588, 826
ApoI RAATTY 1 cut(s) 452
AseI ATTAAT 1 cut(s) 735
AsiGI ACCGGT 1 cut(s) 582
AspLEI GCGC 4 cut(s) 340, 728, 751, 826
AspS9I GGNCC 3 cut(s) 544, 656, 837
AsuC2I CCSGG 2 cut(s) 821, 842
AsuHPI GGTGA 4 cut(s) 198, 310, 550, 762
AvaII GGWCC 2 cut(s) 544, 837
BaeGI GKGCMC 2 cut(s) 562, 813
BanI GGYRCC 1 cut(s) 748
BanII GRGCYC 2 cut(s) 414, 702
BauI CACGAG 1 cut(s) 569
Bbv12I GWGCWC 3 cut(s) 414, 702, 813
BbvI GCAGC 2 cut(s) 575, 813
BccI CCATC 4 cut(s) 142, 187, 529, 782
BceAI ACGGC 1 cut(s) 273
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BciT130I CCWGG 2 cut(s) 236, 507
BcnI CCSGG 2 cut(s) 821, 842
BcoDI GTCTC 1 cut(s) 297
BfaI CTAG 1 cut(s) 795
BfmI CTRYAG 1 cut(s) 31
BfoI RGCGCY 2 cut(s) 752, 827
BfrI CTTAAG 1 cut(s) 98
BisI GCNGC 4 cut(s) 336, 589, 724, 827
BlpI GCTNAGC 1 cut(s) 377
BlsI GCNGC 4 cut(s) 337, 590, 725, 828
Bme1390I CCNGG 4 cut(s) 236, 507, 821, 842
Bme18I GGWCC 2 cut(s) 544, 837
BmeRI GACNNNNNGTC 1 cut(s) 515
BmgT120I GGNCC 3 cut(s) 544, 656, 837
BmiI GGNNCC 3 cut(s) 546, 750, 839
BmrFI CCNGG 4 cut(s) 236, 507, 821, 842
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 1 cut(s) 737
BmuI ACTGGG 1 cut(s) 535
BplI GAGNNNNNCTC 2 cut(s) 294, 326
BpmI CTGGAG 1 cut(s) 398
Bpu1102I GCTNAGC 1 cut(s) 377
BpuMI CCSGG 2 cut(s) 821, 842
Bsa29I ATCGAT 1 cut(s) 731
BsaHI GRCGYC 3 cut(s) 387, 749, 850
BsaJI CCNNGG 6 cut(s) 48, 212, 505, 721, 752, 840
BsaWI WCCGGW 2 cut(s) 348, 582
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 149, 598
Bse118I RCCGGY 3 cut(s) 320, 582, 624
Bse1I ACTGG 2 cut(s) 27, 530
BseBI CCWGG 2 cut(s) 236, 507
BseCI ATCGAT 1 cut(s) 731
BseDI CCNNGG 6 cut(s) 48, 212, 505, 721, 752, 840
BseLI CCNNNNNNNGG 3 cut(s) 49, 149, 598
BseMII CTCAG 2 cut(s) 20, 368
BseNI ACTGG 2 cut(s) 27, 530
BseRI GAGGAG 6 cut(s) 177, 180, 373, 434, 617, 710
BseSI GKGCMC 2 cut(s) 562, 813
BseXI GCAGC 2 cut(s) 575, 813
Bsh1236I CGCG 2 cut(s) 338, 723
BshFI GGCC 2 cut(s) 90, 658
BshNI GGYRCC 1 cut(s) 748
BshTI ACCGGT 1 cut(s) 582
BshVI ATCGAT 1 cut(s) 731
BsiHKAI GWGCWC 3 cut(s) 414, 702, 813
BsiSI CCGG 6 cut(s) 321, 349, 583, 625, 821, 842
BslFI GGGAC 1 cut(s) 592
BslI CCNNNNNNNGG 3 cut(s) 49, 149, 598
BsmAI GTCTC 1 cut(s) 297
BsmFI GGGAC 1 cut(s) 592
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 2 cut(s) 90, 658
Bsp1286I GDGCHC 4 cut(s) 414, 562, 702, 813
Bsp143I GATC 1 cut(s) 175
Bsp1720I GCTNAGC 1 cut(s) 377
BspACI CCGC 4 cut(s) 336, 407, 721, 723
BspANI GGCC 2 cut(s) 90, 658
BspCNI CTCAG 2 cut(s) 19, 369
BspDI ATCGAT 1 cut(s) 731
BspFNI CGCG 2 cut(s) 338, 723
BspLI GGNNCC 3 cut(s) 546, 750, 839
BspPI GGATC 1 cut(s) 170
BspT107I GGYRCC 1 cut(s) 748
BspTI CTTAAG 1 cut(s) 98
BsrFI RCCGGY 3 cut(s) 320, 582, 624
BsrI ACTGG 2 cut(s) 27, 530
BssAI RCCGGY 3 cut(s) 320, 582, 624
BssECI CCNNGG 6 cut(s) 48, 212, 505, 721, 752, 840
BssMI GATC 1 cut(s) 175
BssNI GRCGYC 3 cut(s) 387, 749, 850
BssSI CACGAG 1 cut(s) 569
BssT1I CCWWGG 2 cut(s) 48, 212
Bst2BI CACGAG 1 cut(s) 569
Bst2UI CCWGG 2 cut(s) 236, 507
Bst4CI ACNGT 2 cut(s) 285, 345
Bst6I CTCTTC 1 cut(s) 674
BstACI GRCGYC 3 cut(s) 387, 749, 850
BstAFI CTTAAG 1 cut(s) 98
BstC8I GCNNGC 2 cut(s) 55, 228
BstDEI CTNAG 3 cut(s) 6, 220, 377
BstDSI CCRYGG 1 cut(s) 721
BstENI CCTNNNNNAGG 1 cut(s) 47
BstFNI CGCG 2 cut(s) 338, 723
BstH2I RGCGCY 2 cut(s) 752, 827
BstHHI GCGC 4 cut(s) 340, 728, 751, 826
BstKTI GATC 1 cut(s) 178
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 1 cut(s) 175
BstMWI GCNNNNNNNGC 2 cut(s) 87, 223
BstNI CCWGG 2 cut(s) 236, 507
BstSCI CCNGG 4 cut(s) 234, 505, 819, 840
BstSFI CTRYAG 1 cut(s) 31
BstSLI GKGCMC 2 cut(s) 562, 813
BstUI CGCG 2 cut(s) 338, 723
BstV1I GCAGC 2 cut(s) 575, 813
Bsu15I ATCGAT 1 cut(s) 731
BsuRI GGCC 2 cut(s) 90, 658
BsuTUI ATCGAT 1 cut(s) 731
BtgI CCRYGG 1 cut(s) 721
BtsIMutI CAGTG 2 cut(s) 20, 562
Cac8I GCNNGC 2 cut(s) 55, 228
CfoI GCGC 4 cut(s) 340, 728, 751, 826
Cfr10I RCCGGY 3 cut(s) 320, 582, 624
Cfr13I GGNCC 3 cut(s) 544, 656, 837
Cfr42I CCGCGG 1 cut(s) 724
ClaI ATCGAT 1 cut(s) 731
CseI GACGC 1 cut(s) 395
Csp6I GTAC 5 cut(s) 10, 64, 475, 622, 742
CspAI ACCGGT 1 cut(s) 582
CviAII CATG 3 cut(s) 598, 703, 778
CviQI GTAC 5 cut(s) 10, 64, 475, 622, 742
DdeI CTNAG 3 cut(s) 6, 220, 377
DinI GGCGCC 1 cut(s) 750
DpnI GATC 1 cut(s) 177
DpnII GATC 1 cut(s) 175
DrdI GACNNNNNNGTC 1 cut(s) 239
DriI GACNNNNNGTC 1 cut(s) 515
DseDI GACNNNNNNGTC 1 cut(s) 239
Eam1104I CTCTTC 1 cut(s) 674
Eam1105I GACNNNNNGTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 674
EciI GGCGGA 1 cut(s) 422
Ecl136II GAGCTC 2 cut(s) 412, 700
Eco130I CCWWGG 2 cut(s) 48, 212
Eco24I GRGCYC 2 cut(s) 414, 702
Eco47I GGWCC 2 cut(s) 544, 837
Eco53kI GAGCTC 2 cut(s) 412, 700
Eco57I CTGAAG 2 cut(s) 141, 799
EcoICRI GAGCTC 2 cut(s) 412, 700
EcoNI CCTNNNNNAGG 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 544
EcoRI GAATTC 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 234, 505
EcoT14I CCWWGG 2 cut(s) 48, 212
EcoT38I GRGCYC 2 cut(s) 414, 702
EgeI GGCGCC 1 cut(s) 750
EheI GGCGCC 1 cut(s) 750
ErhI CCWWGG 2 cut(s) 48, 212
FaeI CATG 3 cut(s) 601, 706, 781
FaiI YATR 6 cut(s) 130, 140, 274, 599, 704, 779
FaqI GGGAC 1 cut(s) 592
FatI CATG 3 cut(s) 597, 702, 777
FauI CCCGC 1 cut(s) 728
Fnu4HI GCNGC 4 cut(s) 336, 589, 724, 827
FriOI GRGCYC 2 cut(s) 414, 702
Fsp4HI GCNGC 4 cut(s) 336, 589, 724, 827
FspBI CTAG 1 cut(s) 795
GlaI GCGC 4 cut(s) 339, 727, 750, 825
GluI GCNGC 4 cut(s) 336, 589, 724, 827
GsuI CTGGAG 1 cut(s) 398
HaeII RGCGCY 2 cut(s) 752, 827
HaeIII GGCC 2 cut(s) 90, 658
HapII CCGG 6 cut(s) 321, 349, 583, 625, 821, 842
HgaI GACGC 1 cut(s) 395
HhaI GCGC 4 cut(s) 340, 728, 751, 826
Hin1I GRCGYC 3 cut(s) 387, 749, 850
Hin1II CATG 3 cut(s) 601, 706, 781
Hin6I GCGC 4 cut(s) 338, 726, 749, 824
HinP1I GCGC 4 cut(s) 338, 726, 749, 824
HinfI GANTC 4 cut(s) 297, 605, 791, 803
HpaII CCGG 6 cut(s) 321, 349, 583, 625, 821, 842
HphI GGTGA 4 cut(s) 198, 310, 550, 762
Hpy166II GTNNAC 4 cut(s) 477, 622, 763, 811
Hpy188I TCNGA 6 cut(s) 76, 121, 245, 302, 579, 643
Hpy188III TCNNGA 2 cut(s) 415, 662
Hpy8I GTNNAC 4 cut(s) 477, 622, 763, 811
Hpy99I CGWCG 2 cut(s) 404, 852
HpyAV CCTTC 2 cut(s) 126, 535
HpyCH4III ACNGT 2 cut(s) 285, 345
HpyCH4IV ACGT 2 cut(s) 369, 516
HpyCH4V TGCA 2 cut(s) 57, 811
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 223
HpyF3I CTNAG 3 cut(s) 6, 220, 377
HpySE526I ACGT 2 cut(s) 369, 516
Hsp92I GRCGYC 3 cut(s) 387, 749, 850
Hsp92II CATG 3 cut(s) 601, 706, 781
HspAI GCGC 4 cut(s) 338, 726, 749, 824
KasI GGCGCC 1 cut(s) 748
KspI CCGCGG 1 cut(s) 724
Kzo9I GATC 1 cut(s) 175
LmnI GCTCC 5 cut(s) 190, 409, 417, 697, 844
Lsp1109I GCAGC 2 cut(s) 575, 813
LweI GCATC 1 cut(s) 737
MaeI CTAG 1 cut(s) 795
MaeII ACGT 2 cut(s) 369, 516
MaeIII GTNAC 3 cut(s) 204, 290, 316
MalI GATC 1 cut(s) 177
MboI GATC 1 cut(s) 175
MboII GAAGA 3 cut(s) 63, 376, 691
MhlI GDGCHC 4 cut(s) 414, 562, 702, 813
MluCI AATT 2 cut(s) 452, 666
Mly113I GGCGCC 1 cut(s) 749
MlyI GAGTC 3 cut(s) 306, 614, 785
MmeI TCCRAC 3 cut(s) 41, 223, 280
MseI TTAA 2 cut(s) 99, 735
MslI CAYNNNNRTG 2 cut(s) 137, 464
MspA1I CMGCKG 1 cut(s) 723
MspCI CTTAAG 1 cut(s) 98
MspI CCGG 6 cut(s) 321, 349, 583, 625, 821, 842
MspR9I CCNGG 4 cut(s) 236, 507, 821, 842
Mva1269I GAATGC 1 cut(s) 83
MvaI CCWGG 2 cut(s) 236, 507
MvnI CGCG 2 cut(s) 338, 723
MwoI GCNNNNNNNGC 2 cut(s) 87, 223
NarI GGCGCC 1 cut(s) 749
NciI CCSGG 2 cut(s) 821, 842
NdeII GATC 1 cut(s) 175
NlaIII CATG 3 cut(s) 601, 706, 781
NlaIV GGNNCC 3 cut(s) 546, 750, 839
NmeAIII GCCGAG 1 cut(s) 777
NmuCI GTSAC 2 cut(s) 204, 316
OliI CACNNNNGTG 1 cut(s) 464
PcsI WCGNNNNNNNCGW 1 cut(s) 660
PctI GAATGC 1 cut(s) 83
PfeI GAWTC 1 cut(s) 803
PinAI ACCGGT 1 cut(s) 582
PkrI GCNGC 4 cut(s) 337, 590, 725, 828
PleI GAGTC 3 cut(s) 305, 613, 785
PluTI GGCGCC 1 cut(s) 752
PpsI GAGTC 3 cut(s) 305, 613, 785
PpuMI RGGWCCY 1 cut(s) 544
PshBI ATTAAT 1 cut(s) 735
Psp124BI GAGCTC 2 cut(s) 414, 702
Psp1406I AACGTT 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 544
Psp6I CCWGG 2 cut(s) 234, 505
PspGI CCWGG 2 cut(s) 234, 505
PspN4I GGNNCC 3 cut(s) 546, 750, 839
PspPI GGNCC 3 cut(s) 544, 656, 837
PspPPI RGGWCCY 1 cut(s) 544
RsaI GTAC 5 cut(s) 11, 65, 476, 623, 743
RsaNI GTAC 5 cut(s) 10, 64, 475, 622, 742
RseI CAYNNNNRTG 2 cut(s) 137, 464
SacI GAGCTC 2 cut(s) 414, 702
SacII CCGCGG 1 cut(s) 724
SaqAI TTAA 2 cut(s) 99, 735
SatI GCNGC 4 cut(s) 336, 589, 724, 827
Sau3AI GATC 1 cut(s) 175
Sau96I GGNCC 3 cut(s) 544, 656, 837
SchI GAGTC 3 cut(s) 306, 614, 785
ScrFI CCNGG 4 cut(s) 236, 507, 821, 842
SduI GDGCHC 4 cut(s) 414, 562, 702, 813
SfaNI GCATC 1 cut(s) 737
SfcI CTRYAG 1 cut(s) 31
SfoI GGCGCC 1 cut(s) 750
Sfr303I CCGCGG 1 cut(s) 724
SgrBI CCGCGG 1 cut(s) 724
SinI GGWCC 2 cut(s) 544, 837
SmiMI CAYNNNNRTG 2 cut(s) 137, 464
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 2 cut(s) 452, 666
SsiI CCGC 4 cut(s) 336, 407, 721, 723
SspDI GGCGCC 1 cut(s) 748
SspMI CTAG 1 cut(s) 795
SstI GAGCTC 2 cut(s) 414, 702
StyD4I CCNGG 4 cut(s) 234, 505, 819, 840
StyI CCWWGG 2 cut(s) 48, 212
TaaI ACNGT 2 cut(s) 285, 345
TaiI ACGT 2 cut(s) 372, 519
TaqI TCGA 4 cut(s) 159, 663, 693, 731
TasI AATT 2 cut(s) 452, 666
TatI WGTACW 3 cut(s) 9, 474, 741
TauI GCSGC 2 cut(s) 338, 726
TfiI GAWTC 1 cut(s) 803
Tru1I TTAA 2 cut(s) 99, 735
Tru9I TTAA 2 cut(s) 99, 735
TscAI CASTG 2 cut(s) 27, 562
TseFI GTSAC 2 cut(s) 204, 316
TseI GCWGC 2 cut(s) 588, 826
Tsp45I GTSAC 2 cut(s) 204, 316
TspDTI ATGAA 1 cut(s) 154
TspGWI ACGGA 1 cut(s) 437
TspRI CASTG 2 cut(s) 27, 562
Vha464I CTTAAG 1 cut(s) 98
VneI GTGCAC 1 cut(s) 809
VpaK11BI GGWCC 2 cut(s) 544, 837
VspI ATTAAT 1 cut(s) 735
XagI CCTNNNNNAGG 1 cut(s) 47
XapI RAATTY 1 cut(s) 452
XspI CTAG 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.