RchiOBHm_Chr2g0136251

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0021235
Physical Location & Seq
Reverse (-)
53626991 .. 53627511
521 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50707

Sequence Viewer

Length: 366 bp
ATGGAAAGTGAAGGCTTAACATCTATTTACACCAATGTAGTTACCAAATCTAGCCTACTTAGTGGGGAGGATTCCAAAAGCATGCATGGAAACACGAAAGCGGCATTGGATTACCATGTGTCTATTAACAGTGATTCATATATAGCAACGTATTTTGGAAATATGGATAAGATGGTTGCAGCAATGCGAGCTTTTAATGGCTTGTACAAGACTCTCTTCTTAAGCAGAAGAGCTTTTGCTGAACTCACTTCTCAAGGTTTGAGATGTAAGGAATTGGAGCATGCACTGGGGAAGGCTCACAAAGATGATTTTGTCATGGGAAGAGGATCTGCATTGCCTGATTGCTTTTGTGAGTTCAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

121

Amino Acids

13.39

Weight (kDa)

6.89

Isoelectric Point (pI)

26.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 4 - 65 6.4e-09 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021235)

Species Orthologous Gene IDs
prunus_persica Prupe.4G278800_v2.0.a1 Prupe.4G278800_v2.0.a1 Prupe.4G278800_v2.0.a1 Prupe.4G278800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0136251

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 101
AclWI GGATC 1 cut(s) 334
AfaI GTAC 1 cut(s) 206
AflII CTTAAG 1 cut(s) 220
AgsI TTSAA 1 cut(s) 358
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AluBI AGCT 2 cut(s) 191, 233
AluI AGCT 2 cut(s) 191, 233
AlwI GGATC 1 cut(s) 334
ApeKI GCWGC 1 cut(s) 179
BbvI GCAGC 1 cut(s) 191
BccI CCATC 1 cut(s) 166
BfaI CTAG 1 cut(s) 51
BfrI CTTAAG 1 cut(s) 220
BisI GCNGC 2 cut(s) 102, 180
BlsI GCNGC 2 cut(s) 103, 181
BmrI ACTGGG 1 cut(s) 296
BmuI ACTGGG 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 237
Bse1I ACTGG 1 cut(s) 291
Bse3DI GCAATG 2 cut(s) 189, 332
BseMI GCAATG 2 cut(s) 189, 332
BseNI ACTGG 1 cut(s) 291
BseXI GCAGC 1 cut(s) 191
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 1 cut(s) 326
BspACI CCGC 1 cut(s) 101
BspPI GGATC 1 cut(s) 334
BspQI GCTCTTC 1 cut(s) 223
BspTI CTTAAG 1 cut(s) 220
BsrDI GCAATG 2 cut(s) 189, 332
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 1 cut(s) 291
BssMI GATC 1 cut(s) 326
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 3 cut(s) 221, 223, 316
BstAFI CTTAAG 1 cut(s) 220
BstAUI TGTACA 1 cut(s) 204
BstC8I GCNNGC 3 cut(s) 83, 189, 282
BstDEI CTNAG 1 cut(s) 59
BstKTI GATC 1 cut(s) 329
BstMBI GATC 1 cut(s) 326
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstNSI RCATGY 2 cut(s) 85, 284
BstV1I GCAGC 1 cut(s) 191
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
BtsIMutI CAGTG 2 cut(s) 136, 284
Cac8I GCNNGC 3 cut(s) 83, 189, 282
Csp6I GTAC 1 cut(s) 205
CviAII CATG 5 cut(s) 82, 86, 116, 281, 316
CviJI RGCY 6 cut(s) 15, 54, 191, 201, 233, 296
CviKI_1 RGCY 6 cut(s) 15, 54, 191, 201, 233, 296
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 1 cut(s) 59
DpnI GATC 1 cut(s) 328
DpnII GATC 1 cut(s) 326
Eam1104I CTCTTC 3 cut(s) 221, 223, 316
EarI CTCTTC 3 cut(s) 221, 223, 316
EcoT22I ATGCAT 1 cut(s) 87
FaeI CATG 5 cut(s) 85, 89, 119, 284, 319
FaiI YATR 9 cut(s) 83, 87, 117, 139, 141, 143, 164, 282, 317
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FatI CATG 5 cut(s) 81, 85, 115, 280, 315
Fnu4HI GCNGC 2 cut(s) 102, 180
Fsp4HI GCNGC 2 cut(s) 102, 180
FspBI CTAG 1 cut(s) 51
GluI GCNGC 2 cut(s) 102, 180
Hin1II CATG 5 cut(s) 85, 89, 119, 284, 319
HinfI GANTC 3 cut(s) 71, 134, 211
HpyAV CCTTC 2 cut(s) 5, 286
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 1 cut(s) 149
HpyCH4V TGCA 4 cut(s) 85, 179, 284, 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 149
Hsp92II CATG 5 cut(s) 85, 89, 119, 284, 319
Kzo9I GATC 1 cut(s) 326
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 1 cut(s) 277
LpnPI CCDG 2 cut(s) 272, 351
Lsp1109I GCAGC 1 cut(s) 191
MaeI CTAG 1 cut(s) 51
MaeII ACGT 1 cut(s) 149
MaeIII GTNAC 1 cut(s) 40
MalI GATC 1 cut(s) 328
MboI GATC 1 cut(s) 326
MboII GAAGA 3 cut(s) 208, 240, 333
MflI RGATCY 1 cut(s) 326
MluCI AATT 2 cut(s) 272, 359
MlyI GAGTC 1 cut(s) 205
MnlI CCTC 2 cut(s) 61, 317
Mph1103I ATGCAT 1 cut(s) 87
MseI TTAA 4 cut(s) 17, 126, 195, 221
MslI CAYNNNNRTG 1 cut(s) 303
MspCI CTTAAG 1 cut(s) 220
MwoI GCNNNNNNNGC 1 cut(s) 188
NdeII GATC 1 cut(s) 326
NlaIII CATG 5 cut(s) 85, 89, 119, 284, 319
NsiI ATGCAT 1 cut(s) 87
NspI RCATGY 2 cut(s) 85, 284
PaeI GCATGC 2 cut(s) 85, 284
PciSI GCTCTTC 1 cut(s) 223
PfeI GAWTC 2 cut(s) 71, 134
PkrI GCNGC 2 cut(s) 103, 181
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PsuI RGATCY 1 cut(s) 326
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 303
SapI GCTCTTC 1 cut(s) 223
SaqAI TTAA 4 cut(s) 17, 126, 195, 221
SatI GCNGC 2 cut(s) 102, 180
Sau3AI GATC 1 cut(s) 326
SchI GAGTC 1 cut(s) 205
SetI ASST 4 cut(s) 152, 193, 235, 259
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 2 cut(s) 220, 252
SmoI CTYRAG 2 cut(s) 220, 252
SphI GCATGC 2 cut(s) 85, 284
Sse9I AATT 2 cut(s) 272, 359
SsiI CCGC 1 cut(s) 101
SspMI CTAG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 1 cut(s) 152
TasI AATT 2 cut(s) 272, 359
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 2 cut(s) 71, 134
Tru1I TTAA 4 cut(s) 17, 126, 195, 221
Tru9I TTAA 4 cut(s) 17, 126, 195, 221
TscAI CASTG 2 cut(s) 136, 291
TseI GCWGC 1 cut(s) 179
TspDTI ATGAA 1 cut(s) 126
TspRI CASTG 2 cut(s) 136, 291
Vha464I CTTAAG 1 cut(s) 220
XceI RCATGY 2 cut(s) 85, 284
XspI CTAG 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.