RchiOBHm_Chr2g0155521

Cinnamyl alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0024879
Physical Location & Seq
Reverse (-)
72402338 .. 72404824
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52439

Sequence Viewer

Length: 855 bp
ATGAGCTCCGAAAATAGTACTACAAATGGAAGTGCGAACTGCTTGGGATGGGCTACAAGGGACTCTTTCGGAGTCCTATCGCCTTACAAGTTTAACCGAAGGGATGTCCAAGACGATGACGTTTCCATAAAGATCACACACTGTGGAGTTTGCTATGCTGAGGTTGTTTGGCCAAGGAACAAACATGGAGATGCAACCTATCCTATTGTTCTTGGACATGAGATTGCTGGCATTGTCCAAGAGGTTGGTCCAAATGTTCGTCGATTTAAAGTTGGCGACCATGTTGGAGTTGGAACTTATACCAACTCATGTACAGATCGGTGTACTGCTTCAAAATATCAGAAGAGTATCCATTGGCTTCAGCAGCACCACTGCTTTGTGCTGGCATTACTGTTTACTCTCCCATGCATGATGGACCATAAGATGAACAAACCTGGTAAATCCCTAGGGGTCATTGGCCTAGGCAGTCTTGGTCACATGGCTGTGAAATTCGGCAAGGCCTTCGGACTGAAGGTAACCGTTTTTAGCACAGGTATGTCGAAGAAAGATGAAGCTCTGACTCTGCTTGGGGCAGACAATTTCGTAGCATTGGCTAAGTCACTTGACTTCATTATTGACACAGCATCTGGGAATCACCCGATTGAATTGTATATGTCACTACTGAAGACTGCTGGTGTTCTAGTCCTTGTGGGTGTCCCAAGTGAAATTAAATTGAAACCTTTCAACCTTATTACAGGTAAGAGATCAATTACAGGTAGCGTAGCAGGTGGTACAAAACAAATTCAAGAAATGGTACATTTTTGTGCTGCTCACAAGATATATCCAAACATAGAAGTCGTTCCAATTCAGATGTGA

Protein Analysis

284

Amino Acids

30.9

Weight (kDa)

8.95

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 38 - 113 2.9e-16 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 157 - 269 1.6e-13 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024879)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0155521
rosa_samantha Rh2BG524900 Rh2DG533900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 755
Acc36I ACCTGC 1 cut(s) 755
AcoI YGGCCR 1 cut(s) 170
AcsI RAATTY 2 cut(s) 488, 780
AcuI CTGAAG 3 cut(s) 344, 530, 683
AdeI CACNNNGTG 1 cut(s) 143
AfaI GTAC 5 cut(s) 19, 313, 325, 772, 795
AgsI TTSAA 5 cut(s) 333, 644, 715, 724, 785
AjnI CCWGG 1 cut(s) 433
AluBI AGCT 2 cut(s) 6, 554
AluI AGCT 2 cut(s) 6, 554
Alw21I GWGCWC 1 cut(s) 8
AlwNI CAGNNNCTG 1 cut(s) 626
AoxI GGCC 3 cut(s) 170, 457, 498
ApeKI GCWGC 2 cut(s) 364, 806
ApoI RAATTY 2 cut(s) 488, 780
Asp700I GAANNNNTTC 2 cut(s) 719, 837
AspA2I CCTAGG 2 cut(s) 445, 460
AspS9I GGNCC 2 cut(s) 248, 415
AsuHPI GGTGA 1 cut(s) 626
AvaII GGWCC 2 cut(s) 248, 415
AvrII CCTAGG 2 cut(s) 445, 460
BalI TGGCCA 1 cut(s) 172
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 671
Bbv12I GWGCWC 1 cut(s) 8
BbvCI CCTCAGC 1 cut(s) 159
BbvI GCAGC 2 cut(s) 376, 793
BccI CCATC 2 cut(s) 42, 406
BcgI CGANNNNNNTGC 2 cut(s) 484, 518
BciT130I CCWGG 1 cut(s) 435
BciVI GTATCC 1 cut(s) 359
BfaI CTAG 3 cut(s) 446, 461, 680
BfuAI ACCTGC 1 cut(s) 755
BfuI GTATCC 1 cut(s) 359
BisI GCNGC 2 cut(s) 365, 807
BlnI CCTAGG 2 cut(s) 445, 460
BlsI GCNGC 2 cut(s) 366, 808
BmcAI AGTACT 1 cut(s) 19
Bme1390I CCNGG 1 cut(s) 435
Bme18I GGWCC 2 cut(s) 248, 415
BmgT120I GGNCC 2 cut(s) 248, 415
BmrFI CCNGG 1 cut(s) 435
BmsI GCATC 2 cut(s) 181, 632
BpiI GAAGAC 1 cut(s) 671
Bpu10I CCTNAGC 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 173, 445, 460
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 3 cut(s) 173, 445, 460
BseGI GGATG 2 cut(s) 53, 109
BseMII CTCAG 1 cut(s) 150
BseXI GCAGC 2 cut(s) 376, 793
BshFI GGCC 3 cut(s) 172, 459, 500
BsiHKAI GWGCWC 1 cut(s) 8
BslFI GGGAC 2 cut(s) 74, 680
BsmFI GGGAC 2 cut(s) 74, 680
BsnI GGCC 3 cut(s) 172, 459, 500
Bsp1286I GDGCHC 1 cut(s) 8
Bsp1407I TGTACA 1 cut(s) 311
Bsp143I GATC 3 cut(s) 132, 316, 743
BspANI GGCC 3 cut(s) 172, 459, 500
BspCNI CTCAG 1 cut(s) 151
BspMI ACCTGC 1 cut(s) 755
BsrGI TGTACA 1 cut(s) 311
BssECI CCNNGG 3 cut(s) 173, 445, 460
BssMI GATC 3 cut(s) 132, 316, 743
BssT1I CCWWGG 3 cut(s) 173, 445, 460
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 3 cut(s) 143, 393, 520
Bst6I CTCTTC 1 cut(s) 338
BstAUI TGTACA 1 cut(s) 311
BstC8I GCNNGC 2 cut(s) 229, 384
BstDEI CTNAG 2 cut(s) 159, 594
BstEII GGTNACC 1 cut(s) 514
BstF5I GGATG 2 cut(s) 53, 109
BstKTI GATC 3 cut(s) 135, 319, 746
BstMBI GATC 3 cut(s) 132, 316, 743
BstMWI GCNNNNNNNGC 1 cut(s) 364
BstNI CCWGG 1 cut(s) 435
BstPI GGTNACC 1 cut(s) 514
BstSCI CCNGG 1 cut(s) 433
BstV1I GCAGC 2 cut(s) 376, 793
BstV2I GAAGAC 1 cut(s) 671
BstXI CCANNNNNNTGG 1 cut(s) 245
BsuI GTATCC 1 cut(s) 359
BsuRI GGCC 3 cut(s) 172, 459, 500
BtsCI GGATG 2 cut(s) 53, 109
BtsI GCAGTG 1 cut(s) 370
BtsIMutI CAGTG 2 cut(s) 139, 370
BveI ACCTGC 1 cut(s) 755
Cac8I GCNNGC 2 cut(s) 229, 384
CaiI CAGNNNCTG 1 cut(s) 626
Cfr13I GGNCC 2 cut(s) 248, 415
CsiI ACCWGGT 1 cut(s) 433
Csp6I GTAC 5 cut(s) 18, 312, 324, 771, 794
CviAII CATG 7 cut(s) 185, 218, 281, 309, 405, 409, 478
CviJI RGCY 9 cut(s) 6, 53, 172, 358, 459, 482, 500, 554, 593
CviKI_1 RGCY 9 cut(s) 6, 53, 172, 358, 459, 482, 500, 554, 593
CviQI GTAC 5 cut(s) 18, 312, 324, 771, 794
DdeI CTNAG 2 cut(s) 159, 594
DpnI GATC 3 cut(s) 134, 318, 745
DpnII GATC 3 cut(s) 132, 316, 743
DraI TTTAAA 1 cut(s) 268
DraIII CACNNNGTG 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 170
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 3 cut(s) 173, 445, 460
Eco147I AGGCCT 1 cut(s) 500
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 2 cut(s) 248, 415
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 3 cut(s) 344, 530, 683
Eco91I GGTNACC 1 cut(s) 514
EcoICRI GAGCTC 1 cut(s) 6
EcoO65I GGTNACC 1 cut(s) 514
EcoRII CCWGG 1 cut(s) 433
EcoT14I CCWWGG 3 cut(s) 173, 445, 460
EcoT22I ATGCAT 1 cut(s) 410
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 3 cut(s) 173, 445, 460
FaeI CATG 7 cut(s) 188, 221, 284, 312, 408, 412, 481
FalI AAGNNNNNCTT 2 cut(s) 49, 81
FaqI GGGAC 2 cut(s) 74, 680
FatI CATG 7 cut(s) 184, 217, 280, 308, 404, 408, 477
Fnu4HI GCNGC 2 cut(s) 365, 807
FokI GGATG 2 cut(s) 60, 116
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 2 cut(s) 365, 807
FspBI CTAG 3 cut(s) 446, 461, 680
GluI GCNGC 2 cut(s) 365, 807
HaeIII GGCC 3 cut(s) 172, 459, 500
Hin1II CATG 7 cut(s) 188, 221, 284, 312, 408, 412, 481
HinfI GANTC 4 cut(s) 62, 72, 559, 631
HphI GGTGA 1 cut(s) 626
Hpy166II GTNNAC 2 cut(s) 324, 396
Hpy188I TCNGA 6 cut(s) 10, 71, 342, 506, 558, 849
Hpy188III TCNNGA 1 cut(s) 785
Hpy8I GTNNAC 2 cut(s) 324, 396
Hpy99I CGWCG 1 cut(s) 264
HpyAV CCTTC 3 cut(s) 93, 505, 511
HpyCH4III ACNGT 3 cut(s) 143, 393, 520
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 2 cut(s) 194, 408
HpyF10VI GCNNNNNNNGC 1 cut(s) 364
HpyF3I CTNAG 2 cut(s) 159, 594
HpySE526I ACGT 1 cut(s) 120
Hsp92II CATG 7 cut(s) 188, 221, 284, 312, 408, 412, 481
Kzo9I GATC 3 cut(s) 132, 316, 743
LmnI GCTCC 1 cut(s) 11
Lsp1109I GCAGC 2 cut(s) 376, 793
LweI GCATC 2 cut(s) 181, 632
MabI ACCWGGT 1 cut(s) 433
MaeI CTAG 3 cut(s) 446, 461, 680
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 4 cut(s) 473, 514, 597, 654
MalI GATC 3 cut(s) 134, 318, 745
MboI GATC 3 cut(s) 132, 316, 743
MboII GAAGA 3 cut(s) 355, 553, 676
MhlI GDGCHC 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 172
MluCI AATT 8 cut(s) 488, 577, 644, 705, 710, 747, 780, 843
MluNI TGGCCA 1 cut(s) 172
MlyI GAGTC 3 cut(s) 56, 81, 553
MmeI TCCRAC 2 cut(s) 265, 271
MnlI CCTC 2 cut(s) 154, 235
Mox20I TGGCCA 1 cut(s) 172
Mph1103I ATGCAT 1 cut(s) 410
MroXI GAANNNNTTC 2 cut(s) 719, 837
MscI TGGCCA 1 cut(s) 172
MseI TTAA 3 cut(s) 93, 267, 708
MslI CAYNNNNRTG 4 cut(s) 189, 482, 533, 801
Msp20I TGGCCA 1 cut(s) 172
MspR9I CCNGG 1 cut(s) 435
MvaI CCWGG 1 cut(s) 435
MwoI GCNNNNNNNGC 1 cut(s) 364
NdeII GATC 3 cut(s) 132, 316, 743
NlaIII CATG 7 cut(s) 188, 221, 284, 312, 408, 412, 481
NmuCI GTSAC 3 cut(s) 473, 597, 654
NsiI ATGCAT 1 cut(s) 410
PaqCI CACCTGC 1 cut(s) 755
PceI AGGCCT 1 cut(s) 500
PdmI GAANNNNTTC 2 cut(s) 719, 837
PfeI GAWTC 1 cut(s) 631
PkrI GCNGC 2 cut(s) 366, 808
PleI GAGTC 3 cut(s) 56, 80, 553
PpsI GAGTC 3 cut(s) 56, 80, 553
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 433
PspEI GGTNACC 1 cut(s) 514
PspGI CCWGG 1 cut(s) 433
PspPI GGNCC 2 cut(s) 248, 415
PstNI CAGNNNCTG 1 cut(s) 626
RsaI GTAC 5 cut(s) 19, 313, 325, 772, 795
RsaNI GTAC 5 cut(s) 18, 312, 324, 771, 794
RseI CAYNNNNRTG 4 cut(s) 189, 482, 533, 801
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 3 cut(s) 93, 267, 708
SatI GCNGC 2 cut(s) 365, 807
Sau3AI GATC 3 cut(s) 132, 316, 743
Sau96I GGNCC 2 cut(s) 248, 415
ScaI AGTACT 1 cut(s) 19
SchI GAGTC 3 cut(s) 56, 81, 553
ScrFI CCNGG 1 cut(s) 435
SduI GDGCHC 1 cut(s) 8
SexAI ACCWGGT 1 cut(s) 433
SfaNI GCATC 2 cut(s) 181, 632
SinI GGWCC 2 cut(s) 248, 415
SmiMI CAYNNNNRTG 4 cut(s) 189, 482, 533, 801
Sse9I AATT 8 cut(s) 488, 577, 644, 705, 710, 747, 780, 843
SseBI AGGCCT 1 cut(s) 500
SspMI CTAG 3 cut(s) 446, 461, 680
SstI GAGCTC 1 cut(s) 8
StuI AGGCCT 1 cut(s) 500
StyD4I CCNGG 1 cut(s) 433
StyI CCWWGG 3 cut(s) 173, 445, 460
TaaI ACNGT 3 cut(s) 143, 393, 520
TaiI ACGT 1 cut(s) 123
TaqI TCGA 2 cut(s) 262, 539
TasI AATT 8 cut(s) 488, 577, 644, 705, 710, 747, 780, 843
TatI WGTACW 3 cut(s) 17, 311, 323
TfiI GAWTC 1 cut(s) 631
Tru1I TTAA 3 cut(s) 93, 267, 708
Tru9I TTAA 3 cut(s) 93, 267, 708
TscAI CASTG 2 cut(s) 146, 377
TseFI GTSAC 3 cut(s) 473, 597, 654
TseI GCWGC 2 cut(s) 364, 806
Tsp45I GTSAC 3 cut(s) 473, 597, 654
TspDTI ATGAA 3 cut(s) 440, 564, 598
TspRI CASTG 2 cut(s) 146, 377
VpaK11BI GGWCC 2 cut(s) 248, 415
XapI RAATTY 2 cut(s) 488, 780
XcmI CCANNNNNNNNNTGG 1 cut(s) 287
XmaJI CCTAGG 2 cut(s) 445, 460
XmnI GAANNNNTTC 2 cut(s) 719, 837
XspI CTAG 3 cut(s) 446, 461, 680
ZrmI AGTACT 1 cut(s) 19
Zsp2I ATGCAT 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.